Chungui Lu

Contact

• workRoom C23 The Gateway Building
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• work0115 951 6293
• fax0115 9516292
• chungui.lu@nottingham.ac.uk

Research Summary

Current research concentrates of the use of post-genomic technologies to monitor gene expression profiles/patterns and gene regulatory networks in the following related areas:

1. Development of commercial genetic markers in rice for enhanced nitrogen-use efficiency (ICUK, Joined with Prof. Ping Wu, at Zhejiang University). An integrative systems approach is used on rice varieties to map the genes, understand their effect on nitrogen metabolism and develop genetic markers to facilitate selection of N-efficient lines.

2. Gene regulatory network to investigate vernalization in wheat (BBSRC). We use microarray data to identify target genes which are associated with vernalisation/cold response and functional interactions between genes by systematic functional analysis. The inferred wheat networks (ANNs) showed several highly connected genes which are thought to integrate extracellular signals are regulate growth. These include SPK1, a GTP binding protein and CDF2 transcription factor.

3. Single-cell transcriptomic analysis. Developing new technologies and methods for analysing transcriptomic profiling at the single-cell level (cell sorting and microcapillary) to improve our understanding of the molecular and cellular basis in plant and medical science (e.g. metastasis of cancer and cancer stem cell).

2007-2011, BBSRC CASE Studentships: Gene regulatory network for wheat genotype-dependent effects of Cold Temperatures (Tom Giles)

2007-2011, UoN studentships: The characterisation of novel abscission-related genes in Arabidopsis thaliana (Yang Liu, jointly with Professor Jerry Roberts).

2008-2012, BBSRC CASE studentships and CCFRA Studentships: Manipulating Rab GTPase activity in wheat to affect gluten quality (Adam Tyler, jointly with Dr Grantley Lycett).

2010-2014, IDTC Integrative Biology: Modelling the development of root hairs in rice (Dongbin Liu, jointly with Prof Ping Wu and Dr. Nick Monk).

In collaboration with University of Nottingham, Centre for Plant Integrative Biology (CPIB, http://www.cpib.info/): Transcriptomic profiling at the single-cell level (cell sorting and microcapillary) in Arabidopsis.

Recent Publications

• PAN Y, SEYMOUR GB, LU C, HU Z, CHEN X and CHEN G, 2012. An Ethylene Response Factor (Erf5) Promoting Adaptation To Drought And Salt Tolerance In Tomato. Plant Cell Reports. 31(2), 349-60
• SHI J, LIU M, SHI J, ZHENG G, WANG Y, WANG J, CHEN Y, LU C and YIN W, 2012. Reference Gene Selection For Qpcr In Ammopiptanthus Mongolicus Under Abiotic Stresses And Expression Analysis Of Seven Ros-Scavenging Enzyme Genes. Plant Cell Reports. (In Press.)
• WANG X.F., HE F. F., MA X.X., MAO C.Z., HODGMAN C. and LU C.G. & WU P., 2011. OsCAND1 Is Required for Crown Root Emergence in Rice Molecular Plant.
• PAN Y, CHEN G, LU C and CHEN X. AND HU Z, 2011. Functional Analysis of Tomato LeEIL1 in an Arabidopsis ein2 mutant Acta Physiologiae Plantarum. 33(2), 489-496

• View all publications

• PAN Y, SEYMOUR GB, LU C, HU Z, CHEN X and CHEN G, 2012. An Ethylene Response Factor (Erf5) Promoting Adaptation To Drought And Salt Tolerance In Tomato. Plant Cell Reports. 31(2), 349-60
• SHI J, LIU M, SHI J, ZHENG G, WANG Y, WANG J, CHEN Y, LU C and YIN W, 2012. Reference Gene Selection For Qpcr In Ammopiptanthus Mongolicus Under Abiotic Stresses And Expression Analysis Of Seven Ros-Scavenging Enzyme Genes. Plant Cell Reports. (In Press.)
• WANG X.F., HE F. F., MA X.X., MAO C.Z., HODGMAN C. and LU C.G. & WU P., 2011. OsCAND1 Is Required for Crown Root Emergence in Rice Molecular Plant.
• PAN Y, CHEN G, LU C and CHEN X. AND HU Z, 2011. Functional Analysis of Tomato LeEIL1 in an Arabidopsis ein2 mutant Acta Physiologiae Plantarum. 33(2), 489-496
• TYLER AM,, POOLE M, BHANDARI DG, NAPIER JA, JONES HD, LU C and LYCETT GW, 2011. Manipulating Rab GTPase activity in wheat to improve gluten quality for breadmaking Aspects of Applied Biology.
• JOE A. GALLAGHER, CHUNGUI LU, CHRISTOPHER J. POLLOCK, A. DERI TOMOS, OLGA A. KOROLEVA & JOHN F. FARRAR,, 2011. Rubisco Small Subunit, Chlorophyll a/B-Binding Protein and Sucrose : Fructan-6-Fructosyl Transferase Gene Expression and Sugar Status in Single Barley Leaf Cells in Situ. Cell Type Specificity and Induction by Light. Available at: <http://fws.aber.ac.uk/rec/GALRSS>
• WINFIELD M.O, LU C, WILSON I. D and COGHILL J.A. & EDWARDS K. J., 2010. Plant responses to cold: transcriptome analysis of wheat The Plant Biotechnology Journal. 8, 749-771
• WINFIELD, MARK O, LU, CHUNGUI, WILSON, IAN D, COGHILL, JANE A and EDWARDS, KEITH J, 2009. Cold- and light-induced changes in the transcriptome of wheat leading to phase transition from vegetative to reproductive growth. BMC plant biology. 9, 55
• LINLEY AJ,MILES AK, AL-FAWAZM, LU C, MCARDLE SEB, REES RC, MATHIEU MG, 2009. Non-X-linked cancer/testis antigens: identification, expression profile and immunogenicity Curr Trends Immunol. 10, 49-59
• WINFIELD, MO, LU, CG, WILSON, ID, COGHILL, JA and EDWARDS, KJ, 2009. Cold- And Light-Induced Changes In The Transcriptome Of Wheat Leading To Phase Transition From Vegetative To Reproductive Growth Bmc Plant Biology. 9, -
• HAWKESFORD M. J, LU C, HOWARTH J. R and BARRACLOUGH P. B. AND EDWARDS K, 2007. Microarray analysis of wheat grain transcriptome under nitrogen and sulphur deficiency.: In: Plant nutrition for food security, human health and environmental protection In: Proceedings of the 15th International Plant Nutrition Congress). 80-81
• LU, C., HAWKESFORD, M.J., BARRACLOUGH, P.B., POULTON, P.R., WILSON, I.D., BARKER, G.L. and EDWARDS, K.J., 2005. Markedly different gene expression in wheat grown with organic or inorganic fertilizer Proceedings of the Royal Society B: Biological Sciences. 272(1575), 1901-1908
• WILSON I.D., BARKER G.L.A., LU, C., COGHILL, J.A., BESWICK, R.W., LENTON, J.R. and EDWARDS, K.J., 2005. Alteration of the embryo transcriptome of hexaploid winter wheat (<i>Triticum aestivum</i> cv. Mercia) during maturation and germination Functional & Integrative Genomics. 5(3), 144-154
• HAWKESFORD, M.J., BUCHNER, P., HOWARTH, J.R. and LU, C., 2005. Understanding the regulation of sulfur nutrition - from sulfate transporter genes to the field Phyton. 45, 57
• LU C, HOWARTH JR, BARRACLOUGH PB, EDWARDS KJ AND HAWKESFORD MJ LEIDEN ., 2005. transport and assimilation in plants in the post genomic era: Transcriptome analysis of wheat responses to sulfur nutrition In: Sulfur K Saito, LJ De Kok,. Backhuys Publishers.
• LU C, HAWKESFORD MJ, BARRACLOUGH PB, POULTON PR, WILSON ID, BARKER GL and EDWARDS KJ, 2005. Markedly Different Gene Expression In Wheat Grown With Organic Or Inorganic Fertilizer. Proceedings. Biological Sciences / The Royal Society. 272(1575), 1901-8
• WILSON ID, BARKER GLA, LU C, COGHILL JA, BESWICK RW, LENTON JR and EDWARDS KJ, 2005. Alteration Of The Embryo Transcriptome Of Hexaploid Winter Wheat (Triticum Aestivum Cv. Mercia) During Maturation And Germination. Functional & Integrative Genomics. 5(3), 144-54
• LU, CG, HAWKESFORD, MJ, BARRACLOUGH, PB, POULTON, PR, WILSON, ID, BARKER, GL and EDWARDS, KJ, 2005. Markedly Different Gene With Organic Or Expression In Wheat Grown Inorganic Fertilizer Proceedings Of The Royal Society B-Biological Sciences. 272(1575), 1901-1908
• LU, C., HAWKESFORD, M., WILSON, I., BARKER, G. and EDWARDS, K.J., 2004. Microarray analysis of wheat grain transcriptome-sulphur deficiency Comparative Biochemistry and Physiology. Part A. Comparative Physiology. 137, 7
• WILSON ID, BARKER GLA, BESWICK RW, SHEPHERD SK, LU C, COGHILL JA, EDWARDS D, OWEN P, LYONS R, PARKER JS, LENTON JR, HOLDSWORTH MJ, SHEWRY PR and EDWARDS KJ, 2004. A Transcriptomics Resource For Wheat Functional Genomics. Plant Biotechnology Journal. 2(6), 495-506
• POLLOCK C, FARRAR J, TOMOS D, GALLAGHER J, LU C and KOROLEVA O, 2003. Balancing Supply And Demand: The Spatial Regulation Of Carbon Metabolism In Grass And Cereal Leaves. Journal Of Experimental Botany. 54(382), 489-94
• LU, C., KOROLEVA, O.A., FARRAR, J.F., GALLAGHER, J., POLLOCK, C.J. and TOMOS, A.D., 2002. Rubisco small subunit, chlorophyll <i>a/b</i>-binding protein and sucrose: fructan-6-fructosyl transferase gene expression and sugar status in single barley leaf cells in situ. cell type specificity and induction by light Plant Physiology. 130(3), 1335-1348
• LU C, KOROLEVA OA, FARRAR JF, GALLAGHER J, POLLOCK CJ and TOMOS AD, 2002. Rubisco Small Subunit, Chlorophyll A/B-Binding Protein And Sucrose:Fructan-6-Fructosyl Transferase Gene Expression And Sugar Status In Single Barley Leaf Cells In Situ. Cell Type Specificity And Induction By Light. Plant Physiology. 130(3), 1335-48
• LAVAL V, KOROLEVA OA, MURPHY E, LU C, MILNER JJ, HOOKS MA and TOMOS AD, 2002. Distribution Of Actin Gene Isoforms In The Arabidopsis Leaf Measured In Microsamples From Intact Individual Cells. Planta. 215(2), 287-92
• LU C, ZAINAL Z, TUCKER GA and LYCETT GW, 2001. Developmental Abnormalities and Reduced Fruit Softening in Tomato Plants Expressing an Antisense Rab11 Gtpase Gene. The Plant Cell. 13(8), 1819-33
• LYCETT, G.W., LU, C., ZAINAL, Z. and TUCKER, G.A., 2000. Antisense transformation to investigate the function of a tomato Rab11 GTPase. 6th International Congress of Plant Molecular Biology, Quebec, June 2000 In: XXVIII 5me Journées Statistiques de I'ASU, Quebec, Canada.
• LU,C., LYCETT,G.W. and TUCKER,G.A., 2000. Role of LeRab11a GTP binding protein in plant development and fruit ripening in tomato Journal of Experimental Botany. 51 supp, 26
• LU,C., TUCKER,G.A. and LYCETT,G.W., 1999. The role of ripening induced Rab11-GTP binding protein in tomato Journal of Experimental Botany. 50, 15
• An Ethylene-response Factor (ERF5) Promoting Adaptation to Drought and Salt Tolerance in Tomato Plant Cell Report.