Current Research
I oversee the Proteomics Facility in South lab at Sutton Bonington campus where I perform collaborative investigations with researchers from the University of Nottingham as well as external academic and industrial collaborators.
I perform protein identification by mass spectrometry, as well as additional protein analyses such as accurate intact mass measurement of proteins and covalent/non-covalent interactions.
In the Proteomics Facility, we provide training and guidance in proteomics and protein analysis. We use the core technologies of 2D gel separation and mapping of proteins, followed by identification via mass spectrometry and database searching.
The facility is fully equipped with gel imaging and software analysis tools, robotic assistance including protein spot excision from gels, processing and tryptic digestion to peptides, and autosampler delivery to nanoscale HPLC coupled on-line to the Q-ToF2 mass spectrometer.
Within the Sutton Bonington Proteomics Facility are:-
-Mass spectrometry laboratory
Waters Ltd Q-ToF2, Waters CapLC and LC Packings/Dionex Ultimate Dual Gradient HPLC System
−2D gel laboratory
IEF cell, Protean Xi, Criterion, MPIII, Dodeca MPIII, Gel Imagers [Imager FX (including gel and membrane detection of DiGE and other fluorescent labels), GS800 densitometer], 491 PREPcell (all BioRad) IPGPhorII, MultiPhor, DALT6, DALT2, Processor Plus (all GE Healthcare)
−1D gel and Westerns laboratory
an open-access laboratory. This lab is primarily for researchers within the division of Animal Sciences/South Lab
Researchers from other divisions within the University are welcome to visit and use specialised pieces of equipment not available in their own research labs and for training in 2D gel electrophoresis and other proteomics techniques (see http://www.genomics.nottingham.ac.uk/index.php?option=com_phpshop&Itemid=125).
The Proteomics Facility is part of the University of Nottingham Post-genomic Technology Cluster and the University of Nottingham Interdisciplinary Centre for Analytical Science (UNICAS)
Please contact me directly with any queries ranging from discussion of potential collaborations, training or advice in 2D gel electrophoresis or other proteomics technologies, or for mass spectrometric analysis
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If you plan to submit protein gel bands or spots for MS identification, please see the guidelines for sample preparation
Download the
PROTEIN MASS SPECTROMETRY ANALYSIS REQUEST FORM
Please print and fill this form out as much as possible and bring it to me or send it along with your samples
Here are some Notes on interpreting your de novo sequence data
Where the data we produce contributes towards your research,
please recognise this through appropriate acknowledgement
and / or co-authorship in publications and presentations
Please read the following
Guidelines for appropriate acknowledgement
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Currently working in the 2D gel/MS labs
Dr Ken Davies - research technician in charge of the gel labs. Ken joined the Division of Animal Sciences in 2008 and has trained extensively in Proteomics techniques and is familiar with most of the equipment in the labs. As well as looking after the labs and the people within them, he works on a number of projects, including the cow granulosa cell proteomics project (with Prof Bob Webb). Ken also provides general proteomics advice and runs a practical course for 2D gel electrophoresis.
Adam Williams - a CASE PhD student in the School of Veterinary Medicine and Science & the Division of Animal Sciences, School of Biosciences. Adam is continuing the analysis of secreted proteins from cartilage explant cultures with the aim of identifying biomarkers that are associated with early osteoarthritis. Supervisors: Dr Ali Mobasheri, Dr David Allaway, Waltham Centre for Pet Nutrition/Mars Inc., and Dr Susan Liddell.
Dan Kerruish - a post-doctoral research associate in the Division of Food Sciences, School of Biosciences. Dan is performing 2D gel based proteomic analyses of Sake yeast.
Choulia Mola - a Master of Research student in the Division of Animal Sciences, School of Biosciences. Choulia is using a variety of techniques to investigate protein factors involved in cellular reprogramming. Supervisors: Dr Ramiro Alberio and Dr Susan Liddell.
Irene Nwajei - a Master of Research student in the Divisions of Food Sciences and Animal Sciences, School of Biosciences. Irene is using 2D gel and MS analytical methods to investigate the protein profiles of Ssaccharomyces cerevisiae to inhibitors released from the deconstruction of plant cell walls. Supervisors: Dr Darren Greetham and Dr Susan Liddell.
Zhuolin Sun - a Master of Research student in the Division of Animal Sciences, School of Biosciences. Zhuolin is investigating the proteomics of peripheral and central nervous system tissue in osteoarthritis pain under the supervision of Dr Sara Kelly, Dr Reinhard Stoger, Dr Victoria Chapman, and Dr Susan Liddell.
Past members of the 2D gel/MS labs
Xiaofei Zhang - a student on the Advanced Genomic and Proteomic Sciences Masters course. Xiaofei is working on membrane proteins isolated from articular cartilage chondrocytes. Supervisors: Dr Ali Mobasheri and Dr Susan Liddell.
Abhishek Somani - a PhD student from the Food and Biofuel Innovation Centre. Supervisor: Professor Katherine Smart
Kirsty Hillier - an M.Res student in the School of Veterinary Medicine and Science & the Division of Animal Sciences, School. Kirsty is performing analysis of secreted proteins from cartilage explant cultures with the aim of identifying biomarkers that are associated with early osteoarthritis. Supervisors: Dr Ali Mobasheri, Dr David Allaway, Waltham Centre for Pet Nutrition/Mars Inc., and Dr Susan Liddell. Kirsty now has her M.Res qualification.
Dr Alex Rathbone - using 2D gels to investigate nuclear reprogramming. Under the supervision of Prof. Keith Campbell (Animal Sciences).
Abigail Clutterbuck & Dr Katie Asplin - a PhD student and post-doc from the School of Veterinary Medicine and Science. Performing 2D gel analysis of secreted proteins from cartilage explant cultures (supervisor Dr Ali Mobasheri) with the aim of identifying biomarkers that are associated with early osteoarthritis. Abi has now been examined and is due to graduate with her PhD this summer.
Andrea Rodriguez Martin - a visiting PhD student from The University of Extremadura, Spain - characterisation of secreted fungal proteins, principally by MS analysis. In collaboration with Prof Ian Connerton (Food Sciences). Andrea has recently defended her thesis and will graduate PhD sobresaliente cum laude.
Pete Jones - a PhD student from the Division of Plant and Crop Sciences under the supervision of Prof Mike Holdsworth. Running 2D gels to understand how the N-end rule pathway promotes seed germination and dormancy in Arabidopsis.
Dr Soojin Saa - a visiting researcher from South Korea investigating novel protein factors from granulosa cells associated with the final maturation of GV stage oocytes and during the terminal stages of antral follicle development - with Prof Bob Webb (Agricultural and Environmental Sciences). Soojin recently returned to South Korea to take up a position of Researcher in the National Institute of Animal Science, Chungnam.
Dr. Antoni Borysik - based in Food Sciences (laboratory of David Scott), investigating interactions of ligands to odour binding proteins. Toni occasionally uses the Q-ToF2.
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East Midlands Proteomics Workshop
I co-organise the annual Proteomics Workshop.
The most recent workshop was on
Wednesday 2nd November 2011 at Loughborough University
Further information and on-line registration is available at this www.empw.org.uk
The Workshop is jointly organised by colleagues from the University of Nottingham, Nottingham Trent University, University of Leicester and Loughborough University and acts as a focal point for researchers with a common interest in using proteomic technologies to address fundamental biological and medical questions.
The program for this one-day meeting consists oral presentation sessions along with a poster session and a trade exhibition and is usually attended by over 200 people.