Graham Seymour

Contact

• workRoom C14 Plant Sciences
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• work0115 951 6323
• fax0115 951 6334
• graham.seymour@nottingham.ac.uk

Research Summary

Research Interests

1. Developmental regulation of ripening in fleshy fruits 2. Genetic and molecular basis of fruit quality traits

Research Programme

Fleshy fruits are economically highly valuable and provide a substantial part of the daily intake of vitamins and minerals whether they are consumed in a fresh or processed condition. There is evidence emerging that the genes that regulate ripening in fruits have been conserved during evolution. In my laboratory the aim is to isolate key members of this class of regulatory genes and investigate their role in ripening. There are several strands to the research programme:

Cloning the gene at the Colourless non-ripening (Cnr ) locus.

In tomato a small number of single gene mutations exist, such as rin, nor and Cnr which have pleiotropic effects resulting in the reduction or almost complete abolition of ripening. These probably represent lesions in ripening-regulatory genes. For instance Cnr results in a non-ripening phenotype with two distinct characteristics: (1) firm fruit with reduced cell-to-cell adhesion and (2) complete abolition of carotenoid biosynthesis in the pericarp (see Thompson et al. Plant Physiology 120: 383-389, 1999: Orfila et al. Plant Physiology, 126: 210-221, 2001). Ken Manning (at Warwick HRI) and I have used a genetic map-based approach to isolate the gene at the Cnr locus. Unexpectedly an epimutation governs the expression of the CNR gene. (Manning et al, 2006. Nature Genetics 38, 948 - 952). The CNR gene will provide new insights into the biological basis of juiciness and colour development in fleshy fruits.

Modulation of ripening in non-climacteric fruit using MADS-RIN

Together with Ken Manning at Warwick HRI, and Jim Giovannoni at Cornell I have been working on a strawberry orthologue of the tomato MADS-RIN gene that can modulate ripening in this non-climacteric fruit. Mutant alleles of these genes have previously been used in conventional breeding to enhance texture and shelf-life in commercial tomato.

Conservation of the ripening-regulatory network in fleshy and dry fruits.

We are utilizing information from an advanced genetic framework in the dry fruited Arabidopsis to unravel the control of cell separation and softening in fleshy fruits. One of the tomato genes, TDR4, is a likely orthologue of the Arabidopsis gene FRUITFULL.Related MADS-box genes are associated with ripening in bilberry and apple. In Arabidopsis this MADS-box gene is involved in the control of valve cell differentiation (the cells corresponding to the tomato pericarp). In ful mutants, valve cells adopt the fate of dehiscence zone cells, which are normally programmed to undergo cell separation when the fruit matures. (Ferrándiz, Liljegren and Yanofsky, 2000. Science 289, 436-438). In collaboration with Laura Jaakola from the University of Oulu in Finland we have been investigating the role of a TDR4-like gene in bilberry fruit ripening. The results of this work indicate a major role for bilberry TDR4 in the control of anthocyanin biosynthesis in this fruit (Jaakola et al, 2010. Plant physiology 153, 1619-1629. The Tomato Genome Sequencing Project.

Gerard Bishop and Sarah Butcher at Imperial College, myself at Nottingham, Glenn Bryan at Scottish Crop Research Institute and Jane Rogers at Sanger are spearheading the UK Solanaceae Research Community contribution to the International effort to sequence the tomato genome.

Other projects on-going in the lab include the identification and resolution of QTL for mechanical properties and health-based components in tomato fruits.

Recent Competitive Grants

• ERA-NET Plant Genomics. TomQML Lead PI with Paul Fraser, Alisdair Fernie, Ari Schaffer, Gerco Angenent, Christophe Rothan, James Giovannoni and Industry Partners. 2009-2012.
• BBSRC Systems Biology funding with Charlie Hodgman in CPIB and colleagues at London University and in France. 2008 - 2011.
• EU-SOL Harnessing Biodiversity in the Solanaceae EU PF6 1 2006 to 2010
• Identification of genes that underlie a major QTL for tomato fruit texture KBB/D00103X/1. 2006 to 2009.
• Sequencing the tomato genome (With Gerard Bishop and Sarah Butcher at Imperial, Jane Rogers at Sanger and Glenn Bryan at SCRI) BB/C509731/1. 2005 to 2008.
• A metabolic approach to identification of health based consumer traits in tomato (with Peter Bramley and Paul Fraser at RHUL) BB/D00716X/1. 2006 to 2009
• BBSRC Responsive Mode. A molecular and genetic framework for understanding the regulation of cell adhesion . D20300. 2004 to 2007

Selected Recent Publications

• Seymour,G.B., Ryder C.D., Cevik, V., Hammond, JP., Popovich A., King G.J., Vrebalov J., Giovannoni J.J. and Manning, K. (2011). A SEPALLATA gene is involved in the development and ripening of strawberry (Fragaria x ananassa Duch.) fruit. Journal of Experimental Botany. 62, 1179-1188.
• Jaakola, L., Poole, M., Jones, M.O., Kämäräinen-Karppinen, T., Koskimäki, J.J., Hohtola, A., Häggman, H., Fraser, P.D., Manning, K., King, G.J., Thomson, H. and Seymour, G.B. (2010) A SQUAMOSA MADS-box gene involved in the regulation of anthocyanin accumulation in bilberry fruits. Plant Physiology. 153, 1619-1629 (link)
• Fernandez, A.I., Viron, N., Alhagdow, M., Karimi, M., Jones, M., Amsellem, Z., Sicard, A., Czerednik, A., Angenent, G., Grierson, D., May, S., Seymour, G., Eshed, Y., Lemaire-Chamley, Rothan, C. and Hilson, P. (2009). Flexible tools for gene expression and silencing in tomato. Plant Physiology 151: 1729-1740.
• Vrebalov, J., Pan, I.L., Matas Arroyo., A.J., McQuinn, R., Chung, M-Y., Poole, M., Rose, J., Seymour, G., Grandillo, S., Giovannoni, J., Irish, V.F. (2009) Freshy fruit expansion and ripening are regulated by the tomato SHATTERPROOF gene TAGL1. Plant Cell. 21: 3041-3062 (link)
• Kovacs, K., Fray, R.G., Tikunov, Y., Graham, N., Bradley, G., Seymour, G.B., Bovy, A.G. and Grierson, D. (2009) Effect of tomato pleiotropic ripening mutations on flavour volatile biosynthesis. Phytochemistry 70:1003-1008. (link)
• Mueller, L.A. et al (2009) A snapshot of the emerging tomato genome sequence: the tomato genome sequencing consortium. Plant Genome 2:78-92 (link)
• Cevik, V., Ryder, C.D., Popovich, A., Manning, K., King, G.J. and Seymour, G.B. (2009) A FRUITFULL-like gene is associated with genetic variation for fruit flesh firmness in apple (Malus domestica Borkh.). Tree Genetics & Genomes. doi.10.1007/s11295-009-0247-4 (link)
• Fabi, J.P., Cordenunsi, B.R., Seymour, G.B., Lajola, F.M. and do Nascimento, J.R.O. (2009) Molecular cloning and characterization of a ripening-induced polygalacturonase related to papaya fruit softening. Plant Physiology and Biochemistry 47:1075-1081. (link)
• Seymour, G.B., Manning, K., Poole, M. and King, G.J. (2008). The genetics and epigenetics of fruit development and ripening. Current Opinion in Plant Biology 11:58-63. (link)
• Pilcher, R.L., Moxon, S., Pakseresht, N., Moulton, V., Manning, K., Seymour, G.B. and Dalmay, T. (2007). Identification of novel small RNAs in tomato (Solanum lycopericum). Planta 226:709-717. (link)
• Manning, K., Tor, M., Poole, M., Hong, Y., Thompson, A.J., King, G.J., Giovannoni, J.J. and Seymour, G.B. (2006). A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening. Nature Genetics 38:948-952. (link)
• Barry, C.S., McQuinn, R.P., Thompson, A.J., Seymour, G.B., Grierson, D. and Giovannoni, J.J. (2005). Ethylene insensitivity conferred by Green-ripe and Never-ripe 2 ripening mutants in tomato. Plant Physiology 138:267-275. (link)
• Eriksson, E.M., Bovy, A., Manning, K., Harrison, L., Andrews, J., De Silva, J., Tucker, G.A. and Seymour, G.B. (2004). Effect of the Colourless non-ripening (Cnr) mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening. Plant Physiology 136:4184-4197. (link)
• Marin-Rodriguez, M.C., Smith, D., Manning, K., Orchard, J. and Seymour, S.B. (2003). Pectate lyase gene expression and enzyme activity in ripening banana fruit. Plant Molecular Biology 51:851-857. (link)
• Tor, M., Manning, K., King, G.J., Thompson, A.J., Jones, G.H., Seymour, G.B. and Armstrong, S.J. (2002). Genetic analysis and FISH mapping of the Colourless non-ripening locus of tomato. Theoretical and Applied Genetics 104:165-170. (link)
• Fraser, P.D., Bramley, P. and Seymour, G.B. (2001). Effect of the Cnr mutation on carotenoid formation during tomato fruit ripening. Phytochemistry 58:75-79. (request)
• King, G.J., Lynn, J.R., Dover, C.J., Evans, K.M. and Seymour, G.B. (2001). Resolution of quantitative trait loci for mechanical measures accounting for gentic variation in fruit texture of apple (Malus pumila Mill). Theoretical and Applied Genetics 102:1227-1235. (link)
• Orfila, C., Seymour, G.B., Willats, W.G.T., Huxham, I.M., Jarvis, M.C., Dover, D.J., Thompson, A.J. and Knox, J.P. (2001). Altered middle lamella homogalacturonan and disrupted deposition of (1-5)-a-L-arabinan in the pericarp of Cnr, a ripening mutant of tomato. Plant Physiology 126:210-221. (link)
• Drury, R., Hortensteiner, S., Donnison, I., Bird, C.R. and Seymour, G.B. (1999). Gene expression and chlorophyll catabolism in the peel of ripening banana fruits. Physiologia Plantarum 107:32-38.
• Huxham, I.M., Jarvis, M.C., Shakespeare, L., Dover, C.J., Johnson, D., Knox, J.P. and Seymour, G.B. (1999). Electron energy loss spectroscopic imaging of calcium and nitrogen in the cell walls of apple fruits. Planta 208:438-443. (link)
• Thompson, A.J., Tor, M., Barry, C.S., Vrebalov, J., Orfila, C., Jarvis, M.C., Giovannoni, J.J., Grierson, D. and Seymour, G.B. (1999). Molecular and genetic characterisation of a novel pleiotropic tomato ripening mutant. Plant Physiology 120:383-389. (link)

Selected Publications

• MANNING, K., TOR, M., POOLE M., HONG, Y., THOMPSON, A.J., KING, G.J., GIOVANNONI, J.J. and SEYMOUR, G.B., 2006. A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening Nature Genetics. 38(8), 948-952
• ERIKSSON, E.M., BOVY, A., MANNING, K., HARRISON, L., ANDREWS, J., DE SILVA, J., TUCKER, G.A. and SEYMOUR, G.B., 2004. Effect of the <i>Colorless non-ripening</i> mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening Plant Physiology. 136(4), 4184-4307
• BARRY, C.S., MCQUINN, R.P., THOMPSON, A.J., SEYMOUR, G.B., GRIERSON, D. and GIOVANNONI, J.J., 2005. Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato Plant Physiology. VOL 138(NUMB 1), 267-275

• View all publications

• PAN Y, SEYMOUR GB, LU C, HU Z, CHEN X and CHEN G, 2012. An Ethylene Response Factor (Erf5) Promoting Adaptation To Drought And Salt Tolerance In Tomato. Plant Cell Reports. 31(2), 349-60
• GRAHAM B. SEYMOUR, CAROL D. RYDER, VOLKAN CEVIK, JOHN P. HAMMOND, ALEXANDRA POPOVICH, GRAHAM J. KING, JULIA VERBALOV, JAMES J. GIOVANNONI and KENNETH MANNING, 2011. A SEPALLATA gene is involved in the development and ripening of strawberry (Fragaria x ananassa Duch) fruit, a non-climacteric tissue. Journal of Experimental Botany. 62, 1179-1188
• VOLKAN CEVIK, CAROL D. RYDER, ALEXANDRA POPOVICH, KENNETH MANNING, GRAHAM J. KING and GRAHAM B. SEYMOUR, 2010. A FRUITFULL-like gene is associated with genetic variation for fruit flesh firmness in apple (Malus domestica Borkh). Tree Genetics and Genomes. 6, 271-279
• JAAKOLA L, POOLE M, JONES MO, KÄMÄRÄINEN-KARPPINEN T, KOSKIMÄKI JJ, HOHTOLA A, HÄGGMAN H, FRASER PD, MANNING K, KING GJ, THOMSON H and SEYMOUR GB, 2010. A Squamosa Mads Box Gene Involved In The Regulation Of Anthocyanin Accumulation In Bilberry Fruits. Plant Physiology. 153(4), 1619-29
• KATALIN KOVÁCS, RUPERT G. FRAY, YURY TIKUNOV, NEIL GRAHAM, GLYN BRADLEY, GRAHAM B. SEYMOUR, ARNAUD G. BOVY AND DONALD GRIERSON, 2009. Effect of tomato pleiotropic ripening mutations on flavour volatile biosynthesis Phytochemistry. 70, 1003-1008
• FERNANDEZ, A.I., VIRON, N., ALHAGDOW, M., KARIMI, M., JONES, M., AMSELLEM, Z., SICARD, A., CZEREDNIK, A., ANGENENT, G., GRIERSON, D., MAY, S, SEYMOUR, G, ESHED, Y and LEMAIRE-CHAMLEY, ROTHAN, C. AND H, 2009. Flexible tools for gene expression and silencing in tomato Plant Physiology. doi.10.1104/pp.109.147546
• KATALIN KOVÁCS, RUPERT G. FRAY, YURY TIKUNOV, NEIL GRAHAM, GLYN BRADLEY, GRAHAM B. SEYMOUR, ARNAUD G. BOVY AND DONALD GRIERSON, 2009. Effect of tomato pleiotropic ripening mutations on flavour volatile biosynthesis Phytochemistry. 70, 1003-1008
• CEVIK, V., RYDER, C.D., POPOVICH, A., MANNING, K., KING, G.J. and SEYMOUR, G.B., 2009. A FRUITFULL-like gene is associated with genetic variation for fruit flesh firmness in apple (Malus domestica Borkh.) Tree Genetics and Genomes. doi.10.1007/s11295-009-0247-4
• MUELLER, L.A. ET AL, 2009. A snapshot of the emerging tomato genome sequence: the tomato genome sequencing consortium Plant Genome. 2(1), 78-92
• FABI, J.P., CORDENUNSI, B.R., SEYMOUR, G.B., LAJOLA, F.M. and DO NASCIMENTO, J.R.O., 2009. Molecular cloning and characterization of a ripening-induced polygalacturonase related to papaya fruit softening Plant Physiology and Biochemistry. 47, 1075-1081
• VREBALOV, J., PAN, I.L., MATAS ARROYO, A.J., MCQUINN, R., CHUNG, M-Y., POOLE, M., ROSE, J., SEYMOUR, G., GRANDILLO, S., GIOVANNONI, J. and IRISH, V.F., 2009. Freshy fruit expansion and ripening are regulated by the tomato SHATTERPROOF gene TAGL1 Plant Cell. doi.10.1105/tpc.109.066936
• SEYMOUR G, POOLE M, MANNING K and KING GJ, 2008. Genetics And Epigenetics Of Fruit Development And Ripening. Current Opinion In Plant Biology. 11(1), 58-63
• PILCHER, R.L, MOXON, S., PAKSERESHT, N., MOULTON, V., MANNING, K. and SEYMOUR, G. AND DALMAY, T., 2007. Identification of novel small RNAs in tomato (Solanum lycopersicum) Planta. 226, 709-717
• MANNING, K., TOR, M., POOLE M., HONG, Y., THOMPSON, A.J., KING, G.J., GIOVANNONI, J.J. and SEYMOUR, G.B., 2006. A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening Nature Genetics. 38(8), 948-952
• BARRY, C.S., MCQUINN, R.P., THOMPSON, A.J., SEYMOUR, G.B., GRIERSON, D. and GIOVANNONI, J.J., 2005. Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato Plant Physiology. VOL 138(NUMB 1), 267-275
• MUELLER, L.A, TANKSLEY, S.D, GIOVANNONI J.J, VAN ECK J, STACK, S, CHOI, D, KIM, BD, CHEN, M.S, CHENG ZK, LI, C.Y, LING, H.Q, XUE, Y.B, SEYMOUR, G.B, BISHOP, G, BRYAN, SHARMA, R and KHURANA J., 2005. The tomato sequencing project, the first corner stone of the International Solanaceae Project (SOL). Comparative and Functional Genomics. 6, 153-158
• SEYMOUR, G.B. AND BISHOP, G., 2005. Breeding better varieties through the tomato genome sequencing programme. The Grower. 18-19
• ERIKSSON, E.M., BOVY, A., MANNING, K., HARRISON, L., ANDREWS, J., DE SILVA, J., TUCKER, G.A. and SEYMOUR, G.B., 2004. Effect of the <i>Colorless non-ripening</i> mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening Plant Physiology. 136(4), 4184-4307
• MARIN, C., SMITH, D., MANNING, K., ORCHARD, J. and SEYMOUR, G.B., 2003. Pectate lyase gene expression and enzyme activity in ripening banana fruit. Plant Molecular Biology. 51(6), 851-7
• SEYMOUR, G.B and MANNING, K. AND KING, G.J., 2003. A New Frontier in Fruit Ripening Research. The Grower. 14-15
• SEYMOUR, G.B and DODDS, C. AND TUCKER, G.A., 2003. Cell adhesion in plant, microbial and animal systems. Biotechnology & Genetic Engineering Reviews. 21, 123-130
• TUCKER, GREG and SEYMOUR, GRAHAM, 2002. Life on the vine. Nature Biotechnology. 20(6), 558-60
• SEYMOUR, G. B., MANNING, K., ERIKSSON, E. M., POPOVICH, A. H. and KING, G. J., 2002. Genetic identification and genomic organization of factors affecting fruit texture Journal of Experimental Botany. VOL 53(ISSU 377), 2065-2072
• MARIN-RODRIGUEZ, M. C., ORCHARD, J. and SEYMOUR, G. B., 2002. Pectate lyases, cell wall degradation and fruit softening Journal of Experimental Botany. VOL 53(ISSU 377), 2115-2120
• ORFILA, C., HUISMAN, M. M., WILLATS, W. G., VAN ALEBEEK, G.-J. W., SCHOLS, H. A., SEYMOUR, G. B. and KNOX, J. P., 2002. Altered cell wall disassembly during ripening of Cnr tomato fruit: implications for cell adhesion and fruit softening Planta. VOL 215(PART 3), 440-447
• KNOX, J. P. (J. PAUL) and SEYMOUR, G. B. (GRAHAM B.), 2002. Pectins and their manipulation: edited by Graham B. Seymour and J. Paul Knox Oxford : Blackwell, 2002.
• SEYMOUR, G.B., MANNING, K., ERIKSSON, E.M and POPOVICH A.H. AND KING, GJ, 2002. Genetic identification and genomic organisation of factors affecting fruit texture. Journal of Experimental Botany. 53, 2065-2071
• TOR, M, MANNING, K, KING, G.J, THOMPSON, A.J, JONES, G.H and SEYMOUR, G.B. AND ARMSTRONG, S. J., 2002. Genetic analysis and FISH mapping of the Colourless non-ripening locus of tomato. Theoretical and Applied Genetics. 40, 165-170
• SEYMOUR G.B. AND TUCKER, G.A., 2002. Modification and degradation of pectins.. In: G.B. SEYMOUR & J.P. KNOX, ed., Pectins and their Manipulation Blackwell Publishing. 250 (In Press.)
• SEYMOUR, G. B and NAPIER, R. M. & WHITE, P. J. (EDS), 2002. Fruit development and ripening. Journal of Experimental Botany. 53(Special Issue), 1995-2129
• CAREY, A.T., SMITH, D.L., HARRISON, E., BIRD, C.R., GROSS, K.C., SEYMOUR, G.B. and TUCKER, G.A., 2001. Down-regulation of a ripening-related β-galactosidase gene (TBG1) in transgenic tomato fruits Journal of Experimental Botany. 52(357), 663-668
• ORFILA, C., SEYMOUR, G.B., WILLATS, W.G.T., HUXHAM, I.M., JARVIS, M.C., DOVER, C.J., THOMPSON, A.J. and KNOX, J.P., 2001. Altered middle lamella homogalacturonan and disrupted deposition of (1→5)-<i>ά</i>-L-arabinan in the pericarp of <i>Cnr</i>, a ripening mutant of tomato Plant Physiology. 126(1), 210-221
• SEYMOUR, G. B. AND MANNING, K., 2001. In: M. KNEE, ed., Fruit quality and its biological basis Sheffield Academic Press. 277
• KING, G. J., LYNN, J. R., DOVER, C. J., EVANS, K. M. and SEYMOUR, G. B., 2001. Resolution of quantitative trait loci for mechanical measures accounting for genetic variation in fruit texture of apple (Malus pumila Mill.) Theoretical and Applied Genetics. VOL 102(PART 8), 1227-1235
• JOHNSON, D. S, SEYMOUR, G. B, DOVER, C. J and SAMUELSON, T. J. & SHAKESPEARE, L., 2001. Investigation of textural quality development and post-harvest softening in Cox's Orange Pippin apples In: Innovative Approaches to Improving Food Quality. 1
• SEYMOUR, G. B, TOR, M, MANNING, K, KING, G. J, THOMPSON, A. J and ARMSTRONG, S. J. & JONES, G. H., 2001. FISH mapping on meiotic pachytene chromosome of tomato localises the Cnr gene to the euchromatin of the long arm of chromosome 2 In: Plant & Animal Genome IX Conference.
• JOHNSON, D, DOVER, C, SAMUELSON,T, HUXHAM, I.M, JARVIS, M.C and SHAKESPEARE, L. AND SEYMOUR, G.B., 2001. Nitrogen, cell walls and texture of stored Cox's Orange Pippin Apples Acta Horticulture. 564, 105-112
• FRASER, P D, BRAMLEY, P and SEYMOUR, G B, 2001. Effect of the Cnr mutation on carotenoid formation during tomato fruit ripening. Phytochemistry. 58(1), 75-9
• JOHNSON, D. S, SEYMOUR, G. B, DOVER, C. J and SAMUELSON, T. J. & SHAKESPEARE, L., 2001. Investigation of textural quality development and post-harvest softening in Cox's Orange Pippin apples In: Innovative Approaches to Improving Food Quality. 1
• THOMPSON, A.J., BARRY, C.S., JARVIS, M.C., GIOVANNONI, J.J., GRIERSON, D and SEYMOUR, G.B., 1999. Molecular and genetic characterization of a novel pleiotropic tomato-ripening mutant Plant Physiology. 120(2), 383-90
• BIRD, C. R, FRAY, R. G, GRIERSON, D, SCHUCH, W and SEYMOUR, G. B. & TUCKER, G. A., 1999. Co-ordinated inhibition of plant gene expression US Patent No. US5942 01/01/1900 00:00:00
• DRURY, R., HOERTENSTEINER, S., DONNISON, I., BIRD, C. R. and SEYMOUR, G. B., 1999. Chlorophyll catabolism and gene expression in the peel of ripening banana fruits Physiologia Plantarum. VOL 107(NUMBER 1), 32-38
• JOHNSON, D, DOVER, C and SEYMOUR, G.B. AND SHAKESPEARE, L, 1999. Investigation of textural quality development and postharvest softening in Cox's orange pippin apples APRC News. 22, 6-8
• BIRD, C. R, HOLT, K. A, PICARD, S, SCHUCH, W, CAREY, A. T and SEYMOUR, G. B. & TUCKER, G. A., 1999. Modified fruit containing galactanase transgene. US Patent No. US5859 01/01/1900 00:00:00
• MEDINA-SUAREZ, R, MANNING, K, FLETCHER, J, AKED, J and BIRD, C. R. & SEYMOUR, G. B., 1999. Musa acuminata mRNA; 46 Sequences EMBL/GenBank/DDBJ databases.
• HUXHAM, I. M., JARVIS, M. C., SHAKESPEARE, L., DOVER, C. J., JOHNSON, D., KNOX, J. P. and SEYMOUR, G. B., 1999. Electron-energy-loss spectroscopic imaging of calcium and nitrogen in the cell walls of apple fruits Planta. VOL 208(NUMBER 3), 438-443
• DRURY, R and BIRD, C.R. AND SEYMOUR, G.B., 1998. In: K.E. COCKSHULL, D.GRAY, G.B.SEYMOUR AND B. THOMAS., ed., Genetic and Environmental Manipulation of Horticultural Crops. CAB International, Oxford. 41-49
• COCKSHULL, K.E, GRAY, D and SEYMOUR, G.B. AND THOMAS, eds., 1998. Genetic and Environmental Manipulation of Horticultural Crops. CAB International, Oxford.
• BIRD, C. R and MEDINA-SUAREZ, R. & SEYMOUR, G. B., 1998. Genetic control of fruit ripening World Patent Applica 01/01/1900 00:00:00
• SEYMOUR, G. B. & THOMPSON, A. J., 1997. Potential of molecular biology for manipulating tomato fruit ripening In: HDC/HRA/HRI Tomato Conference. 14
• MEDINA-SUAREZ, R, MANNING, K, FLETCHER, J, AKED, J and BIRD, C. R. & SEYMOUR, G. B., 1997. Musa acuminata mRNA; 46 Sequences EMBL/GenBank/DDBJ databases.
• MEDINA-SUAREZ, R., MANNING, K., FLETCHER, J., AKED, J., BIRD, C. R. and SEYMOUR, G. B., 1997. Gene Expression in the Pulp of Ripening Bananas: Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis of in Vitro Translation Products and cDNA Cloning of 25 Different Ripening-Related mRNAs Plant Physiology. VOL 115(NUMBER 2), 453-461
• JONES, L.J and SEYMOUR, G.B. AND KNOX, J.P., 1997. Characterisation of a monoclonal antibody probe to pectic beta-1,4-galactans and immunolocalisation in tomato cell walls. Plant Physiology. 113, 1405-1412
• SEYMOUR, G.B. AND GROSS, K.C., 1996. Cell wall disassembly and fruit softening. Postharvest News and Information. 7, 45N-52N
• MUDA, P., SEYMOUR, G. B., ERRINGTON, N. and TUCKER, G. A., 1995. Compositional changes in cell wall polymers during mango fruit ripening Carbohydrate Polymers. VOL 26(NUMBER 4), 255
• CAREY, A T, HOLT, K, PICARD, S, WILDE, R, TUCKER, G A, BIRD, C R, SCHUCH, W and SEYMOUR, G B, 1995. Tomato exo-(1-->4)-beta-D-galactanase. Isolation, changes during ripening in normal and mutant tomato fruit, and characterization of a related cDNA clone. Plant Physiology. 108(3), 1099-107
• CAREY, A. T, HOLT, K, PICARD, S, WILDE, R, TUCKER, G. A, BIRD, C. R and SCHUCH, W. AND SEYMOUR, G. B., 1995. Lycopersicon esculentum beta-alactosidase/galactanase protein (putative) EMBL/GenBank/DDBJ databases, Accession no. X83854.
• FENWICK, K.M, JARVIS, M.C, APPERLEY, D.C and SEYMOUR, G.B. AND BIRD, C.R., 1995. Polymer mobility in cell walls of transgenic tomatoes with reduced polygalacturonase activity. Phytochemistry. 42, 301-307
• HALL, L.N., BIRD, C.R., PICTON, S., TUCKER, G.A., SEYMOUR, G.B. and GRIERSON, D., 1994. Molecular characterisation of cDNA clones representing pectin esterase isoenzymes from tomato Plant Molecular Biology. 25, 313-318
• SEYMOUR, G. B., FRAY, R. G., HILL, P. and TUCKER, G. A., 1993. Down-regulation of two non-homologous endogenous tomato genes with a single chimaeric sense gene construct Plant Molecular Biology. VOL 23(NUMBER 1), 1
• HALL, L.N, TUCKER, G.A, SMITH, C.J.S, WATSON, C.F, SEYMOUR, G.B, BUNDICK, Y, BONIWELL, J.M, FLETCHER, J.D, RAY, J.A, SCHUCH, W and BIRD, C.R. AND GRIERSON, D., 1993. Antisense inhibition of pectin esterase gene expression in transgenic tomatoes. The Plant Journal. 3, 121-129
• SEYMOUR, G. B and EDWARDS, R. & HOBSON, G. E. (1993)., 1993. The chelator EDTA overcomes a genetic block to tomato fruit ripening Journal of the Science of Food and Agriculture. 131-132
• SEYMOUR, G.B., 1993. In: G.B. SEYMOUR, J.E. TAYLOR AND G.A. TUCKER, ed., Biochemistry of Fruit Ripening. Chapman and Hall, London, New York. 83-106
• SEYMOUR, G.B. AND MCGLASSON, W.B., 1993. In: G.B. SEYMOUR, J. E. TAYLOR AND G.A. TUCKER, ed., Biochemistry of Fruit Ripening. Chapman and Hall, London, New York. 274-290
• SEYMOUR, G.B. AND TUCKER, G.A., 1993. Avocado. In: G.B. SEYMOUR, J.E. TAYLOR AND G.A. TUCKER, ed., Biochemistry of Fruit Ripening. Chapman and Hall. 53-81
• SEYMOUR, G. B. (GRAHAM B.), TAYLOR, J. E. (JANE E.) and TUCKER, G. A. (GREGORY A.), 1993. Biochemistry of fruit ripening: edited by G. B. Seymour, J. E. Taylor, and G. A. Tucker Chapman & Hall, 1993.
• TUCKER, G.A, SEYMOUR, G.B, LASSLETT, Y, ROBERTSON, D, HALL, L, SMITH, C.J.S, BIRD, C.R and SCHUCH, W. AND GRIERSON, D., 1992. Use of antisense RNA technology to study pectin degradation in tomato fruit. New Zealand Journal of Crop and Horticultural Science. 20, 119-124
• TUCKER, G. A. & SEYMOUR, G. B., 1992. Cell wall degradation during ripening of mango fruit Acta Horticulturae. 291, 454-460
• POGSON, B.J, SEYMOUR, G. B, BRADY, C.J and JONES, M. AND GOODCHILD, D., 1992. Immunolocalisation of pectinases in tomato fruit. New Zealand Journal of Crop and Horticultural Science. 20, 137-146
• SEYMOUR, G.B and WAINWRIGHT, H. AND TUCKER, G.A., 1991. Cell wall changes in ripening fruit Acta Horticulturae. 269, 249-255
• SMITH, N.J, SEYMOUR, G.B and TUCKER, G.A. AND JEGER, M., 1990. Cell wall changes in bananas and plantains Acta Horticulturae. 269, 283-289
• GRIERSON, D, SMITH, C.J.S, WATSON, C.F, MORRIS, P, GRAY, J.E, DAVIES, PICTON, S.J, TUCKER, G.A, SEYMOUR, G.B, SCHUCH, W and BIRD, C.R. AND RAY, J., 1990. In: G.W. LYCELT AND D. GRIERSON, ed., Genetic Engineering of Crop Plants Butterworths, London.
• SEYMOUR, G.B, COLQUHOUN, I.J, DUPONT, S and PARSLEY, K.R. AND SELVENDRAN, R., 1990. Composition and structural features of cell wall polysaccharides from tomato fruits. Phytochemistry. 29, 725-731
• SEYMOUR, G.B and N'DIAYE, M. AND WAINWRIGHT, H., 1990. Effects of cultivar and harvest maturity on ripening of mangoes during storage. Journal of Horticultural Science. 65, 479-483
• SMITH, N. J, SEYMOUR, G. B and TUCKER, G. A. & JEGER, M., 1990. Cell wall changes in bananas and plantains Acta horticulturae. 269, 283-289
• SCHUCH, W., BIRD, C.R., RAY, J., SMITH, C.J., WATSON, C.F., MORRIS, P.C., GRAY, J.E., ARNOLD, C., SEYMOUR, G.B., TUCKER, G.A. and GRIERSON, D., 1989. Control and manipulation of gene expression during tomato fruit ripening Plant Molecular Biology. 13, 303-311
• SMITH, C.J.S, WATSON, C.F, MORRIS P.C, BIRD, C.R, SEYMOUR, G.B, GRAY, J.E, HARDING, S.E, TUCKER, G.A and SCHUCH, W. AND GRIERSON, D., 1989. Inheritance and effect on ripening of antisense polygalacturonase genes in transgenic tomatoes. Plant Molecular Biology. 14, 369-379
• SEYMOUR, G.B and WAINWRIGHT, H. AND TUCKER, G.A., 1989. Cell wall changes in ripening mangoes. Aspects of Applied Biology. 20, 93-94
• SMITH, R and SEYMOUR, G.B. AND TUCKER, G.A., 1989. Inhibition of cell wall degradation by silver (I) ions during ripening of tomato fruit. Journal of Plant Physiology. 13, 514-516
• SY, O and WAINWRIGHT, H. AND SEYMOUR, G.B., 1989. Post-harvest handling of mangoes, a case study from Senegal Aspects of Applied Biology. 20, 95-96
• SEYMOUR, G.B and THOMPSON, A.K. AND JOHN, P., 1987. Inhibition of degreening in the peel of bananas ripened at tropical temperatures. I: Effect of high temperature on changes in the pulp and peel during ripening. Annals of Applied Biology. 110, 145-151
• SEYMOUR, G.B and LASSLETT, Y. AND TUCKER, G.A., 1987. Differential effects of pectolytic enzymes on tomato polyuronides in vivo and in vitro. Phytochemistry. 26, 3137-3139
• SEYMOUR, G.B, HARDING, S.E, TAYLOR, A.J and HOBSON, G.E. AND TUCKER, G.A., 1987. Polyuronide solubilisation during ripening of normal and mutant tomato fruit. Phytochemistry. 26, 1971-1975
• SEYMOUR, G.B and JOHN, P. AND THOMPSON, A.K., 1987. Inhibition of degreening in the peel of bananas ripened at tropical temperatures. II. Role of ethylene, oxygen and carbon dioxide. Annals of Applied Biology. 110, 153-161
• SEYMOUR, G.B. AND HARDING, S.E., 1987. Analysis of the molecular size of tomato fruit polyuronides by gel filtration and low sped sedimentation equilibrium. Biochemical Journal. 245, 463-466
• SMITH, N.J.S and SEYMOUR, G.B. AND THOMPSON, A.K., 1985. Effect of high temperature on the ripening response of bananas to acetylene. Annals of Applied Biology. 108, 667-672
• THOMPSON, A.K. AND SEYMOUR, G.B., 1982. Comparative effects of acetylene and ethylene gas on initiation of banana ripening. Annals of Applied Biology. 101, 407-410
• HUTCHINSON, C.S. AND SEYMOUR, G.B., 1982. Biological flora of the British Isles, Poa annua L. Journal of Ecology. 70, 887-901
• HUGHES, P.A, THOMPSON, A.K and PLUMBLEY, R.A. AND SEYMOUR, G.B., 1981. Storage of capsicums (Capsicum annuum L. (Sendt)) under controlled atmosphere, modified atmosphere and hypobaric conditions. Journal of Horticultural Science. 56(3), 261-265
• SEYMOUR, G.B, THOMPSON, A.K and HUGHES, P.A. AND PLUMBLEY, R.A., 1980. The influence of hydrocooling and plastic box liners on the market quality of capsicums (Capsicum annuum) Acta Horticulturae. 116, 191-196