Neil Graham

Contact

• workRoom C07 Plant Sciences
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• work0115 951 6373
• neil.graham@nottingham.ac.uk

Research Summary

My current research is in plant mineral nutrition. This aim of the research is to improve the nutritional quality of crops. This is focused on the minerals calcium and magnesium in Brassica crop… read more

Recent Publications

• RIOS JJ, LOCHLAINN SÓ, DEVONSHIRE J, GRAHAM NS, HAMMOND JP, KING GJ, WHITE PJ, KURUP S and BROADLEY MR, 2012. Distribution Of Calcium (Ca) And Magnesium (Mg) In The Leaves Of Brassica Rapa Under Varying Exogenous Ca And Mg Supply. Annals Of Botany. (In Press.)
• RAYSON S, ARCIGA-REYES L, WOOTTON L, DE TORRES ZABALA M, TRUMAN W, GRAHAM N, GRANT M and DAVIES B, 2012. A Role For Nonsense-Mediated Mrna Decay In Plants: Pathogen Responses Are Induced In Arabidopsis Thaliana Nmd Mutants. Plos One. 7(2), e31917
• BODI Z, ZHONG S, MEHRA S, SONG J, GRAHAM N, LI H, MAY S and FRAY RG, 2012. Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects Frontiers in Plant Science. 3, 48
• GRAHAM N.S, MAY S.T, DANIEL Z.C.T.R, EMMERSON Z.F, BRAMELD J.M and PARR T., 2011. Use of the Affymetrix Human GeneChip array and Genomic DNA hybridisation probe selection to study ovine transcriptomes Animal. 5(6), 861-866

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Current Research

My current research is in plant mineral nutrition. This aim of the research is to improve the nutritional quality of crops. This is focused on the minerals calcium and magnesium in Brassica crop plants. This is achieved using a combination of approaches including genetic, transcriptomics, functional genomics and comparative genomics.

Future Research

My previous position was is in the Nottingham Arabidopsis Stock Centre, as part of the Affymetrix GenChip service. This involves assisting users of the service with experimental design and data analysis, as well as developing novel analysis techniques. This has led to a number of collaborations with a wide range of groups, both nationally and internationally working on many different plants, animals, yeast and C elegans. Many of these collaborations have resulted from my work in the development of the Xspecies technique. This enables Affymetrix GeneChips to be used with species they were not designed for, using a mixed physical and bioinformatics approach. I have been involved in extending this technique to other systems, including many plant species and animals including sheep and horse studies.

• RIOS JJ, LOCHLAINN SÓ, DEVONSHIRE J, GRAHAM NS, HAMMOND JP, KING GJ, WHITE PJ, KURUP S and BROADLEY MR, 2012. Distribution Of Calcium (Ca) And Magnesium (Mg) In The Leaves Of Brassica Rapa Under Varying Exogenous Ca And Mg Supply. Annals Of Botany. (In Press.)
• RAYSON S, ARCIGA-REYES L, WOOTTON L, DE TORRES ZABALA M, TRUMAN W, GRAHAM N, GRANT M and DAVIES B, 2012. A Role For Nonsense-Mediated Mrna Decay In Plants: Pathogen Responses Are Induced In Arabidopsis Thaliana Nmd Mutants. Plos One. 7(2), e31917
• BODI Z, ZHONG S, MEHRA S, SONG J, GRAHAM N, LI H, MAY S and FRAY RG, 2012. Adenosine methylation in Arabidopsis mRNA is associated with the 3’ end and reduced levels cause developmental defects Frontiers in Plant Science. 3, 48
• GRAHAM N.S, MAY S.T, DANIEL Z.C.T.R, EMMERSON Z.F, BRAMELD J.M and PARR T., 2011. Use of the Affymetrix Human GeneChip array and Genomic DNA hybridisation probe selection to study ovine transcriptomes Animal. 5(6), 861-866
• GRAHAM N and MAY S., 2011. Bioinformatics Resources for Arabidopsis thaliana. In: SCHMIDT R and BANCROFT I., eds., Genetics and Genomics of Brassicaceae 1st ed. Springer. 585-596 (In Press.)
• CARELLI M, BIAZZI E, PANARA F, TAVA A, SCARAMELLI L, PORCEDDU A, GRAHAM N, ODOARDI M, PIANO E, ARCIONI S, MAY S, SCOTTI C, CALDERINI O, 2011. Medicago truncatula CYP716A12 Is a Multifunctional Oxidase Involved in the Biosynthesis of Hemolytic Saponins The Plant Cell. 23, 3070-3081
• ANTONIOU-KOUROUNIOTI M, DE POMERAI D, ANBALAGAN C, LAFAYETTE I, GRAHAM N, GUPTA RP, SINHA P, 2011. Investigation of the effects of dichlorvos on Caenorhabditis elegans gene expression by using reporter transgene strains and qPCR ALTA-Alternatives to laboratory animals. 39(1), 63
• SCOTTI C, CARELLI M, CALDERINI O, PANARA F, GAUDENZI P, BIAZZI E, MAY S, GRAHAM N, PAOLOCCI F, ARCIONI S., 2011. Agronomic and molecular analysis of heterosis in alfalfa Plant Genetic Resources. 9, 288-290
• HAMMOND J.P, MAYES S, BOWEN H.C, GRAHAM N.S, HAYDEN R.M, LOVE C.G, SPRACKLEN W.P, WANG J, WELHAM S.J, WHITE P.J, KING G.J and BROADLEY M.R., 2011. Regulatory hotspots are associated with plant gene expression under varying soil phosphorus (P) supply in Brassica rapa Plant Physiology. 156(3), 1230-1241 (In Press.)
• O LOCHLAINN, SEOSAMH, BOWEN, HELEN C, FRAY, RUPERT G, HAMMOND, JOHN P, KING, GRAHAM J, WHITE, PHILIP J, GRAHAM, NEIL S and BROADLEY, MARTIN R, 2011. Tandem Quadruplication of HMA4 in the Zinc (Zn) and Cadmium (Cd) Hyperaccumulator Noccaea caerulescens. PloS one. 6(3), e17814
• O LOCHLAINN S, AMOAH S, GRAHAM NS, ALAMER K, RIOS JJ, KURUP S, STOUTE A, HAMMOND JH, OSTERGAARD L, KING GJ, WHITE PJ and BROADLEY MR, 2011. High resolution melt (HRM) analysis is an efficient tool to genotype EMS mutants in complex genomes Plant Methods. 7, 43
• GRAHAM, N.S, CLUTTERBUCK A.L, JAMES, N, LEA, R.G, MOBASHERI, A, BROADLEY, M.R and MAY, S.T., 2010. Equine transcriptome quantification using human GeneChip arrays can be improved using genomic DNA hybridisation and probe selection The Veterinary Journal. 186(3), 323-327
• GIBSON B, GRAHAM N, BOULTON C, BOX W, LAWRENCE S, LINFORTH R, MAY S, SMART K., 2010. Differential yeast gene transcription during brewery propagation Journal of the American Society of Brewing Chemists. 68(1), 21-29
• LOVE C.G, GRAHAM N.S, O LOCHLAINN S, BOWN H.C, MAY S.T, WHITE P.J, BROADLEY M.R, HAMMOND J.P and KING G.J., 2010. A Brassica exon array for whole-transcript gene expression profiling PLoS one. (In Press.)
• JONES, BRIAN, GUNNERÅS, SARA ANDERSSON, PETERSSON, SARA V, TARKOWSKI, PETR, GRAHAM, NEIL, MAY, SEAN, DOLEZAL, KAREL, SANDBERG, GÖRAN and LJUNG, KARIN, 2010. Cytokinin Regulation of Auxin Synthesis in Arabidopsis Involves a Homeostatic Feedback Loop Regulated via Auxin and Cytokinin Signal Transduction. The Plant Cell. 22, 2956-2969
• DAVEY, M.A, GRAHAM N.S, VANHOLME, B, SWENNEN, R, MAY, S.T and KEULEMANS, J., 2009. Heterologous oligonucleotide microarrays for transcriptomics in a non-model species; a proof-of-concept study of drought stress in Musa BMC Genomics. 10, 436
• UPTON G, SANCHEZ-GRAILLET O, ROSWELL J, ARTEGA-SALAS J, GRAHAM N, STALTERI M, MEMON F, MAY S, HARRISON A, 2009. On the causes of outliers in Affymetrix GeneChip data Briefings in Functional Genomics and Proteomics. 8, 199-212
• GIBSON, B. R, BOULTON, C. A, BOX, W, GRAHAM, N. S, LAWRENCE, S. J and LINFORTH, R. S. T. & SMART, K. A., 2009. Amino acid uptake and yeast gene transcription during Industrial brewery fermentation Journal of the American Society of Brewing Chemists. 67(3), 157-165
• DAWE, A, BODHICHARLA, R, GRAHAM, N, MAY, S, READER, T, LOADER, B, GREGORY, A, SWICORD, M and BIT-BABIK, G. AND DE POMERAI, D., 2009. Low-intensity microwave irradiation does not substantially alter gene expression in late larval and adult Caenorhabditis elegans. Bioelectromagnetics. 30, 602-612 (In Press.)
• KOVACS, K, TIKUNOV, Y, GRAHAM, N, BRADLEY, G, DEYMOUR, G, BOVY, A and GROERSON, D., 2009. Effect of pleiotropic ripening mutations on flavour volatile biosynthesis Phytochemistry. 70(8), 1003-1008
• BROADLEY MR, WHITE PJ, HAMMOND JP, GRAHAM NS, BOWEN HC, EMMERSON ZF, FRAY RG, IANNETTA PPM, MCNICOL JW and MAY ST, 2008. Evidence of neutral transcriptome evolution in plants. New Phytologist. 180, 587-593
• ROGERS A, MILES A, GRABOWSKA A, GRAHAM N, WATSON S, BISHOP M, REES R., 2008. Functional investigation of T21, a novel cancer-testis antigen, using RNA interference, reveals a role in prostate cancer cell profileration European Urology Supplements. 7(3), 175
• SWARUP, R, BENKOVÁ, E, SWARUP, K, CASIMIRO, I, PÉRET, B, YANG, Y, PARRY, G, NIELSEN, E, DE SMET, I, VANNESTE, S, LEVESQUE, M, CARRIER, D, JAMES, N, CALVO, V, LJUNG, K, KRAMER, E, ROBERTS, R, GRAHAM, N, MARILLONNET, S, PATEL, K, JONES, J, TAYLOR, C, SCHACHTMAN, D, MAY, S, SANDBERG, G, BENFEY, P, FRIML, J, KERR, I, BEECKMAN, T, LAPLAZE, L and BENNETT, M., 2008. The auxin influx carrier LAX3 promotes lateral root emergence. Nature Cell Biology. 10(8), 946-954
• GIBSON, B., LAWRENCE, S., BOULTON, C., BOX, W., GRAHAM, N., LINFORTH, R. and SMART, K., 2008. The oxidative stress response of a lager brewing yeast strain during industrial propagation and fermentation. FEMS Yeast Research. 8(4), 574-585
• GIBSON, B.R., BOULTON, C.A., BOX, W.G., GRAHAM, N.S., LAWRENCE, S.J., LINFORTH, R.S.T. and SMART, K.A., 2008. Carbohydrate utilization and the lager yeast transcriptome during brewery fermentation Yeast. 25, 549-562
• LAPLAZE, L, BENKOVA, E, CASIMIRO, I, MAES, L, VANNESTE, S, SWARUP, R, WEIJERS, D, CALVO, V, PARIZOT, B, HERRERA-RODRIGUEZ, M, OFFRINGA, R, GRAHAM, N, DOUMAS, P, FRIML, J, BOGUSZ, D, BEECKMAN, T and BENNETT, M, 2007. Cytokinins Act Directly on Lateral Root Founder Cells to Inhibit Root Initiation. Plant Cell. 19, 3889-3900
• GRAHAM NS, BROADLEY MR, HAMMOND JP, WHITE PJ and MAY ST, 2007. Optimising the analysis of transcript data using high density oligonucleotide arrays and genomic DNA-based probe selection. BMC Genomics. 8, 344
• JAMES N, GRAHAM N, CLEMENTS D, SCHILDKNECHT B, MAY S, 2007. AtEnsembl - A post-genomic resources browser for Arabidopsis. In: EDWARDS D, ed., Plant Bioinformatics - Methods and Protocols Humana Press. (In Press.)
• CASIMIRO, I., BEECKMAN, T., GRAHAM, N., BHALERAO, R., ZHANG, H., CASERO, P., SANDBERG, G. and BENNETT, M. J., 2003. Dissecting Arabidopsis lateral root development Trends in Plant Science. VOL 8(NUMBER 4), 165-171
• SWARUP, R., PARRY, G., GRAHAM, N., ALLEN, T. and BENNETT, M., 2002. Auxin cross-talk: integration of signalling pathways to control plant development Plant Molecular Biology. VOL 49(NUMBER 3/4), 409-424
• CASIMIRO, I., MARCHANT, A., BHALERAO, R.P., BEECKMAN, T., DHOOGE, S., SWARUP, R., GRAHAM, N., INZÉ, D., SANDBERG, G., CASERO, P.J. and BENNETT, M., 2001. Auxin transport promotes Arabidopsis lateral root initiation Plant Cell. 13(4), 843-852
• PARRY, G., MARCHANT, A., MAY, S., SWARUP, R., SWARUP, K., JAMES, N., GRAHAM, N.S., ALLEN, T., MARTUCCI, T., YEMM, A., NAPIER, R., MANNING, K., KING, G. and BENNETT, M.J., 2001. Quick on the uptake: characterization of a family of plant auxin influx carriers Journal of Plant Growth Regulation. 20, 217
• MAATHUIS, F. J. M., MAY, S. T., GRAHAM, N. S., BOWEN, H. C., JELITTO, T. C., TRIMMER, P., BENNETT, M. J., SANDERS, D. and WHITE, P. J., 1998. Cell marking in Arabidopsis thaliana and its application to patch-clamp studies Plant Journal. VOL 15(NUMBER 6), 843-851