Duncan Hannant

Contact

• workRoom B25 Veterinary Academic Building
  Sutton Bonington Campus
  Sutton Bonington
  Leicestershire
  LE12 5RD
  UK
• work0115 951 6455
• fax0115 951 6440
• duncan.hannant@nottingham.ac.uk

Biography

My mainstream research and publications are in the area of immune responses to infection with and vaccination against respiratory viruses in horses, with extended interests in agricultural species and dogs.Understanding the horse's immune responses to natural infection has been critical to the development of new strategies for disease prevention. For example, my group described the locally recirculating mucosal immune system in horses and we exploited this to demonstrate for the first time that intransal vaccination with inactivated virus and mucosal adjuvants induced extremely effective immunity to re-infection with equine influenza virus. Detailed studies with my colleague Dr Julia Kydd (also at SVMS Nottingham) have uniquely identified cellular immune correlates of protection from alphaherpesviruses in horses. This new information has led to rational approaches to new vaccine design. Both of these developments have had significant benefits to the health and welfare of horses.

Other active research areas are allergic immune responses in domestic dogs to housedust mites (Canine Atopic Dermatitis).

In addition to these research areas, my current funding supports the development of immunological reagents specifically for the horse as part of a BBSRC initiative on Combatting Viral Diseases of Livestock (The Immunological Toolbox; http://www.immunologicaltoolbox.co.uk ) . New funding has been won from the EU, FP& Programme currently under contract negotiation. Novel technologies for Surveillance of Emerging and Re-emerging Infections of Wildlife (Acronym = WildTech). Total budget €8.1M, total requested from EU €5.9M, total award to University of Nottingham €1.4M. Co-applicants from Nottingham are Drs Paul Barrow, Lisa Yon, Richard Lea (SVMS) and Mrs S Boardman (EMZS, Twycross Zoo, Clinical Associate of the School). There are 11 international partners mainly in EU and one in Canada. Anticipated start date for the project is Jan/Feb 2009.

Expertise Summary

Research expertise is in a wide range of laboratory and field investigations of immune responses to infectious viruses in animals. Successful in winning many competitive research awards to fund research and support postgraduate (PhD and MSc) students. Director of studies for 10 successful PhD and 3 MSc studnets. Approx 20 years experience of managing research groups and active member of senior management/strategic groups. Regular reviewer of research applications on behalf of the major funding bodies and extenal PhD examiner for universities of Cambridge, London, Edinburgh.

Research Summary

PI on BBSRC grant The Immunological Toolbox (part of the Combating Viral Diseases of Livestock Initiative) 2004-2008. Specialist area Equine Immunology. Part of consortium (pigs, cattle, sheep,… read more

Recent Publications

• PAILLOT, R., DALY, J.M., LUCE, R., MONTESSO, F., DAVIS-POYNTER, N., HANNANT, D. and KYDD, J.H., 2007. Frequency and phenotype of EHV-1 specific, IFN-γ synthesising lymphocytes in ponies: the effects of age, pregnancy and infection Developmental and Comparative Immunology. 31(2), 202-214
• PAILLOT R, KYDD JH, MACRAE S, MINKE JM, HANNANT D, DALY JM, 2007. New assays to measure equine influenza virus-specific Type 1 immunity in horses. Vaccine. 25(42), 7385-98
• KYDD, J.H., DAVIS-POYNTER, N.J., BIRCH, J., HANNANT, D., MINKE, J., AUDONNET, J.-C., ANTCZAK, D.F. and ELLIS, S A., 2006. A molecular approach to the identification of cytotoxic T-lymphocyte epitopes within equine herpesvirus 1 Journal of General Virology. 87(9), 2507-2515
• PAILLOT, R., ELLIS, S.A., DALY, J.M., AUDONNET, J.C., MINKE, J. M., DAVIS-POYNTER, N., HANNANT, D. and KYDD, J.H., 2006. Characterisation of CTL and IFN-g synthesis in ponies following vaccination with a NYVAC-based construct coding for EHV-1 immediate early gene, followed by challenge infection Vaccine. VOL 24(NUMBER 10), 1490-1500

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Current Research

PI on BBSRC grant The Immunological Toolbox (part of the Combating Viral Diseases of Livestock Initiative) 2004-2008. Specialist area Equine Immunology. Part of consortium (pigs, cattle, sheep, chickens, horses) linked with BBSRC Institutes of Animal Health and Moredun Research Institute (SEERAD)

Coordinator of EU FP7 Grant currently under contract negotiation. Novel technologies for Surbveillance of Emerging and Re-emerging Infections of Wildlife (Acronym = WildTech). Total budget €8.1M, total requested from EU €5.9M, total award to University of Nottingham €1.4M. Co-applicants from Nottingham are Drs Paul Barrow, Lisa Yon, Richard Lea (SVMS) and Mrs S Boardman (EMZS, Twycross Zoo, Clinical Associate of the School). There are 11 international partners mainly in EU and one in Canada. Anticipated start date for the project is Jan/Feb 2009.

Past Research

Any area of veterinary immunology, in particular respiratory diseases, vaccinology and canine atopic dermatitis.

• PAILLOT, R., DALY, J.M., LUCE, R., MONTESSO, F., DAVIS-POYNTER, N., HANNANT, D. and KYDD, J.H., 2007. Frequency and phenotype of EHV-1 specific, IFN-γ synthesising lymphocytes in ponies: the effects of age, pregnancy and infection Developmental and Comparative Immunology. 31(2), 202-214
• PAILLOT R, KYDD JH, MACRAE S, MINKE JM, HANNANT D, DALY JM, 2007. New assays to measure equine influenza virus-specific Type 1 immunity in horses. Vaccine. 25(42), 7385-98
• KYDD, J.H., DAVIS-POYNTER, N.J., BIRCH, J., HANNANT, D., MINKE, J., AUDONNET, J.-C., ANTCZAK, D.F. and ELLIS, S A., 2006. A molecular approach to the identification of cytotoxic T-lymphocyte epitopes within equine herpesvirus 1 Journal of General Virology. 87(9), 2507-2515
• PAILLOT, R., ELLIS, S.A., DALY, J.M., AUDONNET, J.C., MINKE, J. M., DAVIS-POYNTER, N., HANNANT, D. and KYDD, J.H., 2006. Characterisation of CTL and IFN-g synthesis in ponies following vaccination with a NYVAC-based construct coding for EHV-1 immediate early gene, followed by challenge infection Vaccine. VOL 24(NUMBER 10), 1490-1500
• KYDD, J. H., TOWNSEND, H. G. and HANNANT, D., 2006. The equine immune response to equine herpesvirus-1: The virus and its vaccines Veterinary Immunology and Immunopathology. VOL 111(NUMBER 1-2), 15-30
• PAILLOT, R., KYDD, J. H., SINDLE, T., HANNANT, D., EDLUND TOULEMONDE, C., AUDONNET, J. C., MINKE, J. M. and DALY, J. M., 2006. Antibody and IFN-g responses induced by a recombinant canarypox vaccine and challenge infection with equine influenza virus Veterinary Immunology and Immunopathology. VOL 112(NUMBER 3-4), 225-233
• PAILLOT, R., HANNANT, D., KYDD, J. H. and DALY, J. M., 2006. Vaccination against equine influenza: Quid novi? Vaccine. VOL 24(NUMBER 19), 4047-4061
• MITTELHOLZER, C., STADEJEK, T., JOHANSSON, I., BAULE, C., CIABATTI, I., HANNANT, D., PATON, D., AUTORINO, G. L., NOWOTNY, N. and BELAK, S., 2006. Extended Phylogeny of Equine Arteritis Virus: Division into New Subgroups JOURNAL OF VETERINARY MEDICINE -BERLIN- SERIES B-. VOL 53(NUMBER 2), 55-58
• PAILLOT, R., DALY, J.M., JUILLARD, V., MINKE, J.M., HANNANT, D. and KYDD, J.H., 2005. Equine interferon gamma synthesis in lymphocytes after in vivo infection and in vitro stimulation with EHV-1 Vaccine. 23(36), 4541-4551
• CROUCH, C. F., DALY, J., HENLEY, W., HANNANT, D., WILKINS, J. and FRANCIS, M. J., 2005. The use of a systemic prime/mucosal boost strategy with an equine influenza ISCOM vaccine to induce protective immunity in horses Veterinary Immunology and Immunopathology. VOL 108(NUMBER 3-4), 345-355
• SHAW, S. C., WOOD, J. L. N., FREEMAN, J., LITTLEWOOD, J. D. and HANNANT, D., 2004. Estimation of heritability of atopic dermatitis in Labrador and Golden Retrievers American Journal of Veterinary Research. VOL 65(PART 7), 1014-1020
• CROUCH, C. F., DALY, J., HANNANT, D., WILKINS, J. and FRANCIS, M. J., 2004. Immune responses and protective efficacy in ponies immunised with an equine influenza ISCOM vaccine containing an `American lineage' H3N8 virus Vaccine. VOL 23(NUMBER 3), 418-425
• SHAW, S. C., WOOD, J. L. N., FREEMAN, J., LITTLEWOOD, J. D. and HANNANT, D., 2004. Estimation of heritability of atopic dermatitis in Labrador and Golden Retrievers JOURNAL- AMERICAN VETERINARY MEDICAL ASSOCIATION. VOL 225(PART 2), 237-237
• CASTILLO-OLIVARES, J., TEARLE, J.P., MONTESSO, F., WESTCOTT, D., KYDD, J.H., DAVIS-POYNTER, N.J. and HANNANT, D., 2003. Detection of equine arteritis virus (EAV)-specific cytotoxic CD8+ T-lymphocyte precursors from EAV-infected ponies Journal of General Virology. 84(10), 2745-2753
• KYDD, J.H., WATTRANG, E. and HANNANT, D., 2003. Pre-infection frequencies of equine herpesvirus-1 specific, cytotoxic T lymphocytes correlate with protection against abortion following experimental infection of pregnant mares Veterinary Immunology and Immunopathology. 96(3-4), 207-217
• WATTRANG, E., JESSETT, D., YATES, P., FUXLER, L. and HANNANT, D., 2003. Experimental Infection of Ponies with Equine Influenza A2 (H3N8) Virus Strains of Different Pathogenicity Elicits Varying Interferon and Interleukin-6 Responses VIRAL IMMUNOLOGY. VOL 16(NUMBER 1), 57-67
• WESTCOTT, D. G., KING, D. P., DREW, T. W., NOWOTNY, N., KINDERMANN, J., HANNANT, D., BELAK, S. and PATON, D. J., 2003. Use of an internal standard in a closed one-tube RT-PCR for the detection of equine arteritis virus RNA with fluorescent probes VETERINARY RESEARCH. VOL 34(PART 2), 165-176
• TEARLE, J. P., SMITH, K. C., PLATT, A. J., HANNANT, D., DAVIS-POYNTER, N. J. and MUMFORD, J. A., 2003. In vitro characterisation of high and low virulence isolates of equine herpesvirus-1 and -4 Research in Veterinary Science. VOL 75(NUMBER 1), 83-86
• HOROHOV, D. W., KYDD, J. H. and HANNANT, D., 2002. The effect of aging on T cell responses in the horse Developmental and Comparative Immunology. VOL 26(NUMBER 1), 121-128
• HANNANT, D., 2002. Mucosal immunology: overview and potential in the veterinary species VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY. VOL 87(NO 3-4), 265-268
• NORDENGRAHN, A., MERZA, M., ROS, C., LINDHOLM, A., PALFI, V., HANNANT, D. and BELAK, S., 2002. Prevalence of equine herpesvirus types 2 and 5 in horse populations by using type-specific PCR assays VETERINARY RESEARCH. VOL 33(PART 3), 251-260
• HELDENS, J. G., HANNANT, D., CULLINANE, A. A., PRENDERGAST, M. J., MUMFORD, J. A., NELLY, M., KYDD, J. H., WESTSTRATE, M. W. and VAN DEN HOVEN, R., 2001. Clinical and virological evaluation of the efficacy of an inactivated EHV1 and EHV4 whole virus vaccine (Duvaxyn EHV1,4). Vaccination/challenge experiments in foals and pregnant mares Vaccine. VOL 19(NUMBER 30), 4307 - 4317
• CASTILLO-OLIVARES, J., DE VRIES, A. A. F., RAAMSMAN, M. J. B., ROTTIER, P. J. M., LAKHANI, K., WESTCOTT, D., TEARLE, J. P., WOOD, J. L. N., MUMFORD, J. A. and HANNANT, D., 2001. Evaluation of a prototype sub-unit vaccine against equine arteritis virus comprising the entire ectodomain of the virus large envelope glycoprotein (G~L): induction of virus-neutralizing antibody and assessment of protection in ponies Journal of General Virology. VOL 82(PART 10), 2425-2435
• SLATER, J. and HANNANT, D., 2000. Equine Immunity to Viruses VETERINARY CLINICS OF NORTH AMERICA EQUINE PRACTICE. VOL 16(PART 1), 49-68
• SMITH, K. C., WHITWELL, K. E., MUMFORD, J. A., HANNANT, D., BLUNDEN, A. S. and TEARLE, J. P., 2000. Virulence of the V592 isolate of Equid Herpesvirus-1 in Ponies JOURNAL OF COMPARATIVE PATHOLOGY. VOL 122(PART 4), 288-297
• NEWTON, J. R., TOWNSEND, H. G. G., WOOD, J. L. N., SINCLAIR, R., HANNANT, D. and MUMFORD, J. A., 2000. Immunity to equine influenza: relationship of vaccine-induced antibody in young Thoroughbred racehorses to protection against field infection with influenza A/equine-2 viruses (H3N8) Equine Veterinary Journal. VOL 32(PART 1), 65-74
• HANNANT, D., O'NEILL, T., OSTLUND, E. N., KYDD, J. H., HOPKIN, P. J. and MUMFORD, J. A., 1999. Equid Herpesvirus Induced Immunosuppression is Associated with Lymphoid Cells and Not Soluble Circulating Factors VIRAL IMMUNOLOGY. VOL 12(PART 4), 313-322
• O'NEILL, T., KYDD, J.H., ALLEN, G.P., WATTRANG, E., MUMFORD, J.A. and HANNANT, D., 1999. Determination of equid herpesvirus 1-specific, CD8^+, cytotoxic T lymphocyte precursor frequencies in ponies Veterinary Immunology and Immunopathology. VOL 70(NUMBER 1-2), 43-54
• KYDD, J. H., ALLEN, G. P., O NEILL, T., WATTRANG, E. and HANNANT, D., 1998. EHV-1-specific, cytotoxic lymphocyte precursor frequencies following intranasal infection of ponies EQUINE INFECTIOUS DISEASES. 8TH, 412
• FLORE, P. H., MINKE, J. M., MUMFORD, J. A., HANNANT, D., JESSETT, D., VANDEHOEK, J. and WESTSTRATE, M. W., 1998. Studies on the efficacy of an inactivated, carbomer-adjuvanted equine herpesvirus vaccine in pregnant mares in the face of a challenge with an abortigenic strain of EHV-1 EQUINE INFECTIOUS DISEASES. 8TH, 422-423
• HANNANT, D. and WATTRANG, E., 1998. Development of reagents for equine interferons EQUINE INFECTIOUS DISEASES. 8TH, 486-488
• INIGUEZ, P., ZIENTARA, S., MARAULT, M., BIRCH MACHIN, I., HANNANT, D. and CRUCIERE, C., 1998. Screening of horse polyclonal antibodies with a random peptide library displayed on phage: identification of ligands used as antigens in an ELISA test to detect the presence of antibodies to equine arteritis virus Journal of Virological Methods. VOL 73(ISSUE 2), 175-183
• MUMFORD, J. A., JESSETT, D., HANNANT, D., DALY, J., UPPAL, P., YADAV, M. P. and WEBSTER, R. G., 1998. A model system for investigating immunity stimulated by live equine influenza vaccines EQUINE INFECTIOUS DISEASES. 8TH, 44-49
• BINNS, M., HANNANT, D., WOOD, J. L. N. and MUMFORD, J. A., 1998. Genetic influence on immune responses to equine vaccines EQUINE INFECTIOUS DISEASES. 8TH, 477-478
• HANNANT, D., 1998. Phagocytosis and Flow Cytometric Analyses: Another Step Towards an Urgent Goal The Veterinary Journal. VOL 156(NUMBER 2), 79-80
• HANNANT, D., EASEMAN, R. and MUMFORD, J. A., 1998. Equine mucosal immune system: intranasal vaccination with inactivated equine influenza virus protects from infection EQUINE INFECTIOUS DISEASES. 8TH, 50-56
• CASTILLO-OLIVARES, J., BIRCH-MACHIN, I., CHIRNSIDE, E. D., RYDER, S., BINNS, M. M., HANNANT, D. and MUMFORD, J. A., 1998. Application of phage display to epitope mapping of the large envelope glycoprotein (G~L) of equine arteritis virus using horse antiserum to a synthetic peptide EQUINE INFECTIOUS DISEASES. 8TH, 442-443
• HANNANT, D. and MUMFORD, J. A., 1997. Rational Approaches to Design and Evaluation of Equine Vaccines PROCEEDINGS OF THE DUBAI INTERNATIONAL EQUINE SYMPOSIUM. 321-334
• KYDD, J. H., HANNANT, D. and MUMFORD, J. A., 1996. Residence and recruitment of leucocytes to the equine lung after EHV-1 infection Veterinary Immunology and Immunopathology. VOL 52(NUMBER 1/2), 15-26
• HANNANT, D., EASEMAN, R. L., EVERS, E. and MUMFORD, J. A., 1996. Mucosal vaccination with inactivated equine influenza virus stimulates neutralising antibody in the nasopharynx of horses Veterinary Immunology and Immunopathology. VOL 54(NUMBER 1/4), 205-206
• DOLBY, C. A., HANNANT, D. and MUMFORD, J. A., 1995. Response of Ponies to Adjuvanted EHV-1 Whole Virus Vaccine and Challenge with Virus of the Homologous Strain BRITISH VETERINARY JOURNAL. VOL 151(NUMBER 1), 27
• BLUNDEN, A. S., HANNANT, D., LIVESAY, G. and MUMFORD, J. A., 1994. Susceptibility of ponies to infection with Streptococcus pneumoniae (capsular type 3) Equine Veterinary Journal. VOL 26(NUMBER 1), 22
• FORD, DAVID MALCOLM and UNIVERSITY OF NOTTINGHAM. THESES. COMPUTER SCIENCE, 1991. On-line recognition of connected handwriting