Bennett Lab Members - Lee Garratt

* European Commission Programme (EC FP5): NATURAL

Nutritional Genomics: Combining metabolic profiling and quantitative genetics to pinpoint key genes regulating vitamin accumulation in plants (Swarup et al., 2002; Garratt et al., 2003; Garratt et al., 2005).

• MAFF sponsored LINK Agro Food Quality Programme (AFQ): Antioxidant research

The creation and assessment of novel lettuce germplasm with enhanced oxyradical scavenging potential and higher antioxidant levels (Garratt et al., 2001; 2002; 2003)

• European Commission Programme (EC RTD): Improved quality and shelf-life in fruit and vegetables

The application of two novel molecular strategies for the quality improvement of produce; The creation the molecular and physiological assessment of novel lettuce germplasm containing an autoregulatory senescence-inhibition system (PSAG12-ipt) and a prokaryotic asparagines synthetase gene, asn-A (Garratt et al., 2000; McCabe et al., 2001; Davey et al., 2002; Garratt et al., 2002; Davey et al., 2003; Garratt et al., 2003).

PRESENTATIONS

Lab Meeting, Combining metabolite profiling and quantitative genetics to pinpoint key genes regulating vitamin accumulation in plants, Nottingham, 22/11/2004.

Metabolomics workshop presentation, Combining Metabolite Profiling and Quantitative Genetics to Pinpoint Key Genes Regulating Vitamin Accumulation in Plants, Nottingham, 2003.

NATURAL EU presentation, High Through-Put Mapping using SNP Analysis, Confirmation of QTL for Ascorbate Accumulation, and Folate Analysis, Versailles, 11/2002.

TARGET presentation, Research in Antioxidants, Nottingham, 2002

Lab meeting, Combining metabolite profiling and quantitative genetics to pinpoint key genes regulating vitamin accumulation in plants, Nottingham, 14/7/2003.

Lab meeting, Targeted metabolite profiling of folate accumulation and biosynthesis in plants, Nottingham, 17/11/2003.

Plant Science Seminar, Targeted metabolite profiling of folate accumulation and biosynthesis in plants, Nottingham, 18/2/2004.

POSTERS

EURO"2-FEDA conference poster, Antioxidants: Benefits and Risks, Cambridge, 9/2002.

SELECTED PUBLICATIONS

RESEARCH PAPERS

Garratt, L. C., Ortori, C. A., Tucker, G. A., Sablitzky, F., Bennett, M. J. and Barrett, D. A. (2005) Comprehensive metabolic profiling of mono- and polyglutamated folates and their precursors in plant and animal tissue using liquid chromatography-negative ion electrospray ionisation tandem mass spectrometry. Rapid Communications in Mass Spectrometry 19: 2390-2398

Garratt, L. C., Linforth, R., Taylot, A. J, Lowe K., Power J. B and Davey, M. R. (2005) Metabolite fingerprinting in transgenic lettuce. Plant Biotechnology Journal 3: 165-174.

Garratt, L. C., Anthony, P., Power, J. B., Davey, M. R. and Lowe, K. C. (2003) Growth and antioxidant status of plant cells cultured with bovine haemoglobin solution. Advances in Experimental Medicine and Biology 540: 163-169.

Garratt, L. C., Janagoudar, B. S., Anthony, P., Davey, M. R., Power, J. B. and Lowe, K. C. (2002) Salinity tolerance and status in cotton cultures. Free Radical Biology and Medicine 33, 502-511.

Garratt L. C, Davey M. R, McCabe M. S and Power J. B. (2002b), Enhancement of crop performance and quality in lettuce. Buletinul USAMV-CN, 55/2001

Bÿhm, H., Anthony, P., Garratt., L. C., Briarty, L. G., Lowe. K. C., Power, J. B., Benes, E. and Davey, M. R. (2002) Ultrasound-induced physiological changes in cultured cells of Petunia Hybrida. Artificial Cells, Blood Substitutes and Immobilization Biotechnology 30, 127-136.

Garratt, L. C., Janagoudar, B. S., Anthony, P., Davey, M. R., Power, J. B. and Lowe, K. C. (2001) Hemoglobin-stimulated growth and antioxidant activities in cultured cotton cells. Free Radical Biology and Medicine 31, 1156-1162.

McCabe, M. S., Garratt, L. C., Schepers, F., Jordi, W. J. R. M., Stoopen, G. M., Davelaar, E., van Rhijn, J. H. A., Power, J. B. and Davey, M. R. (2001) Effect of PSAG12-IPT gene expression on development and senescence in transgenic lettuce. Plant Physiology 127, 505-516.

BOOK CHAPTERS

Davey M. R., Garratt L. C., Williams, F. M. K. and Power, J. B. (2003) In vitro regeneration and transformation of the leafy vegetables lettuce, chicory and spinach. In: Plant genetic engineering Vol 4: Improvement of vegetables and fruits (Eds. Jaiwal PK and Singh RP). Dordrecht, Kluwer Academic Publishers, pp 133-175.

Garratt, L. C., Power, J. B. and Davey M. R. (2002) Plant modification to improvement of the shelf-life of vegetables by genetic manipulation. In: Fruit and Vegetable Processing: Improving Quality (Ed, Jongen W). Cambridge, UK and CRC Press, Bocc Raton, New York, Washington DC., pp 267-287.

Garratt, L. C., McCabe, M. S., Power, J. B. and Davey, M. R. (2001) Detection of single-copy genes in DNA from transgenic plants. In: Methods in Molecular Biology. Vol 179, Gene Probes: Principles and Protocols (Eds. Aquino M and Rapley R). Humana Press, Totowa, NY: pp 211-221.

PROCEEDINGS

Garratt, L. C., Ortori, C. A., Tucker, G, A., Barrett, D. A. and Bennett, M. J. (2004) Using metabolic profiling to exploit natural variation for the enhancement of folates. Comparative Biochemistry and Physiology, Vol 137A (3) S151

Garratt, L. C., Swarup, K., Tucker, G. A. and Bennett, M. J. (2003) Using nutritional genomics to identify key loci that regulate ascorbate pool size. Comparative Biochemistry and Physiology, Vol 134A (3) S213

Garratt, L. C., Wiley, P., Power, J. B., Lowe, K. C. and Davey, M. R. (2003) Genetic manipulation of lettuce to delay senescence and improve crop quality and yield. Eucapia Leafy Vegetable Conference, pp 159.

Davey, M. R., Williams, F. M. K., Garratt, L. C., Anthony, P. and Power, J. B. (2003) Genetic modification of lettuce, chicory and spinach. Eucapia Leafy Vegetable Conference, pp 161.

Garratt, L. C., Linforth, R., Taylor, A. J., Lowe, K. C., Power, J. B. and Davey, M. R. (2003) Metabolite Profiling in Transgenic Lettuce, Comparative Biochemistry and Physiology, Vol 134A (3) S154

Swarup, K., Garratt, L. C., Tucker, G. A. and Bennett, M. J. (2002) Nutritional Genomics: Identification of key loci that regulate ascorbate pool size. Free Radical Research, 36, S1, 106.

Garratt, L. C., Creissen, G., McCabe, M. S., Mullineaux, P., Jimenez, A., Kular, B., Power, J. B. and Davey, M. R. (2001) Effects of altered glutathione biosynthesis and metabolism in transgenic lettuce (Lactuca sativa L). J Exp Bot, 52 (suppl), 11.

Janagoudar, B. S., Garratt, L. C., Lowe, K. C., Davey, M. R. and Power, J. B. (2001) Hemoglobin-stimulated growth and antioxidant activities in cultured cotton cells, J Exp Bot, 52 (suppl), 12.

Janagoudar, B. S., Garratt, L. C., Lowe, K. C., Davey, M. R. and Power, J. B. (2001) Salinity tolerance in cotton tissue cultures: antioxidant enzyme biosynthesis, J Exp Bot, 52 (suppl), 13.