Tim Self

Contact

• workRoom C89A The University of Nottingham Medical School
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work0115 82 30090
• fax0115 82 30081
• tim.self@nottingham.ac.uk
• http://www.nottingham.ac.uk/ics/facilities_and_services/confocal_imaging/confocal_imaging.php

Expertise Summary

I have over 20years experience in electron microscopy (TEM and SEM), light microscopy and their preparation techniques. My major interests are in confocal microscopy with particular emphasis on live cell imaging.

Research Techniques/Expertise

• Confocal
• FRAP, FLIP,FRET
• Live cell confocal imaging
• High Content Screening
• TEM & SEM
• Immunocytochemistry

• Cell culture

Interests

My main interests are using confocal microscopy to study membrane receptors. Over recent years I have studied the trafficking and cycling of GFP tagged-receptors using both live and fixed cells. Future directions lie in developing existing confocal systems and the development of our high content screening platforms.

Publications

I have published in a number of journals, for example Development, Developmental Biology, Nature, Nature Neuroscience, Science, Current Biology, Journal of Pathology, British Journal of Pharmocology, Journal of Clinical Investigation and Molecular Therapy.

Equipment/ systems

• Zeiss 710 confocal microscope
• Zeiss LSM510uv META combi confocal microscope
• Zeiss NLO META Combi 2 Photon confocal microscope
• Zeiss LSm Exciter confocal with TIRF
• Zeiss Confocor FCS microscope
• MDC confocal and widefield imaging plate readers
• MDC Flexstation

Research Summary

Expertise in Confocal and live cell imaging, electron and light microscopy, cell culture.

Selected Publications

• SELF, T, MAHONY, M, FLEMING, J, WALSH, J, BROWN, S D and STEEL, K P, 1998. Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cells. Development (Cambridge). 125(4), 557-66
• SELF, T.J., OAKLEY, S.M. and HILL, S.J., 2005. Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells British Journal of Pharmacology. 146(4), 612-24
• KROS, C J, MARCOTTI, W, VAN NETTEN, S M, SELF, T J, LIBBY, R T, BROWN, S D M, RICHARDSON, G P and STEEL, K P, 2002. Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutations. Nature Neuroscience. 5(1), 41-7
• ORIHUELA, C, MAHDAVI, J, THORNTON, J, MANN, B, WOOLDRIDGE, K.G, ABOUSEADA, N, OLDFIELD, N.J, SELF, T, ALA'ALDEEN, D.A.A and TUOMANEN, E.I, 2009. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. Journal of Clinical Investigation. 119, 1638-1646

• View all publications

Future Research

I am in the process of developing a High Content Screening facility incorporating MDC confocal and widefield plate readers, a plate loading robot and image analysis and archiving system.

• HAIDER AJ, BRIGGS D, SELF TJ, CHILVERS HL, HOLLIDAY ND and KERR ID, 2011. Dimerization Of Abcg2 Analysed By Bimolecular Fluorescence Complementation. Plos One. 6(10), e25818
• JAMES, VICTORIA, ZHANG, YINING, FOXLER, DANIEL E, DE MOOR, CORNELIA H, KONG, YI WEN, WEBB, THOMAS M, SELF, TIM J, FENG, YUNGFENG, LAGOS, DIMITRIOS, CHU, CHIA-YING, RANA, TARIQ M, MORLEY, SIMON J, LONGMORE, GREGORY D, BUSHELL, MARTIN and SHARP, TYSON V, 2010. LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing. Proceedings of the National Academy of Sciences of the United States of America. 107(28), 12499-12504
• MAY, LT, SELF, TJ, BRIDDON, SJ and HILL, SJ, 2010. The Effect Of Allosteric Modulators On The Kinetics Of Agonist-G Protein-Coupled Receptor Interactions In Single Living Cells Molecular Pharmacology. 78(3), 511-523
• GHOSH TK, SONG FF, PACKHAM EA, BUXTON S, ROBINSON TE, RONKSLEY J, SELF T, BONSER AJ, BROOK JD., 2009. Physical interaction between TBX5 and MEF2C is required for early heart development. Molecular And Cellular Biology.. 29(8), 2205-2218
• ORIHUELA, C, MAHDAVI, J, THORNTON, J, MANN, B, WOOLDRIDGE, K.G, ABOUSEADA, N, OLDFIELD, N.J, SELF, T, ALA'ALDEEN, D.A.A and TUOMANEN, E.I, 2009. Laminin receptor initiates bacterial contact with the blood brain barrier in experimental meningitis models. Journal of Clinical Investigation. 119, 1638-1646
• ANDERSON, D., SELF, T., MELLOR, I.R., GOH, G., HILL, S.J. and DENNING, C., 2007. Transgenic enrichment of cardiomyocytes from human embryonic stem cells. Molecular Therapy. 15(11), 2027-2036
• DENNING, C., ALLEGRUCCI, C., PRIDDLE, H., BARBADILLO-MUÑOZ, M.D., ANDERSON, D., SELF, T., SMITH, N.M., PARKIN, C.T. and YOUNG, L.E., 2006. Common culture conditions for maintenance and cardiomyocyte differentiation of the human embryonic stem cell lines, BG01 and HUES-7. International Journal of Developmental Biology. 50(1), 27-37
• SELF, T.J., OAKLEY, S.M. and HILL, S.J., 2005. Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells British Journal of Pharmacology. 146(4), 612-24
• GOH, G., SELF, T., BARBADILLO MUÑOZ, M.D., HALL, I.P., YOUNG, L. and DENNING, C., 2005. Molecular and phenotypic analyses of human embryonic stem cell-derived cardiomyocytes: opportunities and challenges for clinical translation Thrombosis and Haemostasis. 94(4), 728-737
• QAZI, S.N.A., HARRISON, S.E., SELF, T., WILLIAMS, P. and HILL, P.J., 2004. Real-time monitoring of intracellular Staphylococcus aureus replication Journal of Bacteriology. 186(4), 1065-1077
• KROS, C J, MARCOTTI, W, VAN NETTEN, S M, SELF, T J, LIBBY, R T, BROWN, S D M, RICHARDSON, G P and STEEL, K P, 2002. Reduced climbing and increased slipping adaptation in cochlear hair cells of mice with Myo7a mutations. Nature Neuroscience. 5(1), 41-7
• MAKANYA, A N, SELF, T J, WARUI, C N and MWANGI, D K, 2001. Gut morphology and morphometry in the epauletted Wahlberg's fruit bat (Epomophorus wahlbergi, Sundevall, 1846). Acta Biologica Hungarica. 52(1), 75-89
• SELF, T, SOBE, T, COPELAND, N G, JENKINS, N A, AVRAHAM, K B and STEEL, K P, 1999. Role of myosin VI in the differentiation of cochlear hair cells. Developmental Biology. 214(2), 331-41
• SELF, T, MAHONY, M, FLEMING, J, WALSH, J, BROWN, S D and STEEL, K P, 1998. Shaker-1 mutations reveal roles for myosin VIIA in both development and function of cochlear hair cells. Development (Cambridge). 125(4), 557-66
• STEEL, K P, MBURU, P, GIBSON, F, WALSH, J, VARELA, A, BROWN, K, SELF, T, MAHONY, M, FLEMING, J, PEARCE, A, HARVEY, D, CABLE, J and BROWN, S D, 1997. Unravelling the genetics of deafness. Annals of Otology, Rhinology and Laryngology (Supplement). 168, 59-62
• MAYHEW, T M, MWAMENGELE, G L, SELF, T J and TRAVERS, J P, 1994. Stereological studies on red corpuscle size produce values different from those obtained using haematocrit- and model-based methods. British Journal of Haematology. 86(2), 355-60
• LASZLO, L, LOWE, J, SELF, T, KENWARD, N, LANDON, M, MCBRIDE, T, FARQUHAR, C, MCCONNELL, I, BROWN, J and HOPE, J, 1992. Lysosomes as key organelles in the pathogenesis of prion encephalopathies. Journal of Pathology. 166(4), 333-41
• LáSZLó, L, DOHERTY, F J, WATSON, A, SELF, T, LANDON, M, LOWE, J and MAYER, R J, 1991. Immunogold localisation of ubiquitin-protein conjugates in primary (azurophilic) granules of polymorphonuclear neutrophils. FEBS Letters. 279(2), 175-8
• LáSZLó, L, TUCKWELL, J, SELF, T, LOWE, J, LANDON, M, SMITH, S, HAWTHORNE, J N and MAYER, R J, 1991. The latent membrane protein-1 in Epstein-Barr virus-transformed lymphoblastoid cells is found with ubiquitin-protein conjugates and heat-shock protein 70 in lysosomes oriented around the microtubule organizing centre. Journal of Pathology. 164(3), 203-14
• WALLER, D A, THOMAS, N W and SELF, T J, 1988. Epithelial restitution in the large intestine of the rat following insult with bile salts. Virchows Archiv. A: Pathological anatomy and histopathology. 414(1), 77-81