Scientist
Research interests
Jeroen Stoof started his study at the D epartment of Laboratory Science and Chemical Engineering, the Utrecht University of Professional Education (HLO), Utrecht, The Netherlands, with as specialisation Medical Microbiology. After an internship studying molecular typing of hospital-acquired infections at the Department of Medical Microbiology and Infection Prevention at the Vrije Universiteit Amsterdam, he started as technician at the same department in 1997, working in the group of Dr. Savelkoul. In 2001, he moved to the Department of Gastroenterology and Hepatology of the Erasmus MC - University Medical Center Rotterdam to start a job as r esearch technician on the metal-responsive gene regulation in Helicobacter pylori under supervision of Dr. Arnoud van Vliet and Prof. Ernst Kuipers . From 2005 onwards he has combined a position of PhD student and research technician at the same department, with a research project on acid resistance and metal-responsive gene regulation in pathogenic Helicobacter species. In 2010 he has started a position as postdoctoral research associate at MBIG group, focusing on host pathogen interaction of Campylobacter jejuni.
Publications
Stoof, J., E. J. Kuipers, G. Klaver, and A. H. M. van Vliet. 2010. TonB2 and an ABC transporter contribute to Helicobacter mustelae nickel and cobalt acquisition. Infect Immun 2010, Epub ahead of print, 19 July 2010 (DOI: 10.1128/IAI.00365-10).
Stoof, J., E. J. Kuipers, and A. H. M. van Vliet. 2010. Characterization of NikR-responsive promoters of urease and metal transport genes of Helicobacter mustelae. Biometals 23:145-59.
Belzer, C., J. Stoof, S. Breijer , J. G. Kusters, E. J. Kuipers, and A. H. M. van Vliet. 2009. The Helicobacter hepaticus hefA gene is involved in resistance to amoxicillin. Helicobacter 14:72-9.
Moons, L. M., J. G. Kusters, J. H. van Delft, E. J. Kuipers, R. Gottschalk, H. Geldof, W. A. Bode, J. Stoof, A. H. M. van Vliet, H. B. Ketelslegers, J. C. Kleinjans, and P. D. Siersema. 2008. A pro-inflammatory genotype predisposes to Barrett's esophagus. Carcinogenesis 29:926-31.
Stoof, J., C. Belzer, and A. H. M. van Vliet. 2008. Metal metabolism and transport in Helicobacter pylori . In Y. Yamaoka (ed.), Helicobacter pylori, Molecular Genetics and Cellular Biology . Caister Academic Press, Norfolk, UK, 165-177.
Stoof, J., S. Breijer , R. G. J. Pot, D. van der Neut, E. J. Kuipers, J. G. Kusters, and A. H. M. van Vliet. 2008. Inverse nickel-responsive regulation of two urease enzymes in the gastric pathogen Helicobacter mustelae. Environ Microbiol 10:2586-97.
Belzer, C., J. Stoof, and A. H. M. van Vliet. 2007. Metal-responsive gene regulation and metal transport in Helicobacter species. Biometals 20:417-29.
Pot, R. G. J., J. Stoof, P. J. Nuijten, L. A. de Haan, P. Loeffen, E. J. Kuipers, A. H. M. van Vliet, and J. G. Kusters. 2007. UreA2B2: a second urease system in the gastric pathogen Helicobacter felis. FEMS Immunol Med Microbiol 50:273-9.
Ernst, F. D., J. Stoof, W. M. Horrevoets, E. J. Kuipers, J. G. Kusters, and A. H. van Vliet. 2006. NikR mediates nickel-responsive transcriptional repression of the Helicobacter pylori outer membrane proteins FecA3 (HP1400) and FrpB4 (HP1512). Infect Immun 74:6821-8.
Gerrits, M. M., A. P. Godoy, E. J. Kuipers, M. L. Ribeiro, J. Stoof, S. Mendonca, A. H. M. van Vliet, J. Pedrazzoli, Jr., and J. G. Kusters. 2006. Multiple mutations in or adjacent to the conserved penicillin-binding protein motifs of the penicillin-binding protein 1A confer amoxicillin resistance to Helicobacter pylori. Helicobacter 11:181-7.
Belzer, C., J. Stoof, C. S. Beckwith, E. J. Kuipers, J. G. Kusters, and A. H. M. van Vliet. 2005. Differential regulation of urease activity in Helicobacter hepaticus and Helicobacter pylori. Microbiology 151:3989-95.
Ernst, F. D., S. Bereswill, B. Waidner, J. Stoof, U. Mader, J. G. Kusters, E. J. Kuipers, M. Kist, A. H. M. van Vliet, and G. Homuth. 2005. Transcriptional profiling of Helicobacter pylori Fur- and iron-regulated gene expression. Microbiology 151:533-46.
Ernst, F. D., G. Homuth, J. Stoof, U. Mader, B. Waidner, E. J. Kuipers, M. Kist, J. G. Kusters, S. Bereswill, and A. H. M. van Vliet. 2005. Iron-responsive regulation of the Helicobacter pylori iron-cofactored superoxide dismutase SodB is mediated by Fur. J Bacteriol 187:3687-92.
Ernst, F. D., E. J. Kuipers, A. Heijens, R. Sarwari, J. Stoof, C. W. Penn, J. G. Kusters, and A. H. M van Vliet. 2005. The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori. Infect Immun 73:7252-8.
de Jonge, R., E. J. Kuipers, S. C. Langeveld, R. J. Loffeld, J. Stoof, A. H. M. van Vliet, and J. G. Kusters. 2004. The Helicobacter pylori plasticity region locus jhp0947-jhp0949 is associated with duodenal ulcer disease and interleukin-12 production in monocyte cells. FEMS Immunol Med Microbiol 41:161-7.
van Vliet, A. H. M., E. J. Kuipers, J. Stoof, S. W. Poppelaars, and J. G. Kusters. 2004. Acid-responsive gene induction of ammonia-producing enzymes in Helicobacter pylori is mediated via a metal-responsive repressor cascade. Infect Immun 72:766-73.
de Vries, N., E. M. van Ark, J. Stoof, E. J. Kuipers, A. H. M. van Vliet, and J. G. Kusters. 2003. The stress-induced hsp12 gene shows genetic variation among Helicobacter pylori strains. FEMS Immunol Med Microbiol 38:45-51.
van Vliet, A. H. M. , J. Stoof, S. W. Poppelaars, S. Bereswill, G. Homuth, M. Kist, E. J. Kuipers, and J. G. Kusters. 2003. Differential regulation of amidase- and formamidase-mediated ammonia production by the Helicobacter pylori fur repressor. J Biol Chem 278:9052-7.
van Vliet, A. H. M., S. W. Poppelaars, B. J. Davies, J. Stoof, S. Bereswill, M. Kist, C. W. Penn, E. J. Kuipers, and J. G. Kusters. 2002. NikR mediates nickel-responsive transcriptional induction of urease expression in Helicobacter pylori. Infect Immun 70:2846-52.
van Vliet, A. H. M., J. Stoof, R. Vlasblom, S. A. Wainwright, N. J. Hughes, D. J. Kelly, S. Bereswill, J. J. Bijlsma, T. Hoogenboezem, C. M. Vandenbroucke-Grauls, M. Kist, E. J. Kuipers, and J. G. Kusters. 2002. The role of the Ferric Uptake Regulator (Fur) in regulation of Helicobacter pylori iron uptake. Helicobacter 7:237-44.
Koeleman, J. G., J. Stoof, M. W. Van Der Bijl, C. M. Vandenbroucke-Grauls, and P. H. Savelkoul. 2001. Identification of epidemic strains of Acinetobacter baumannii by integrase gene PCR. J Clin Microbiol 39:8-13.
Koeleman, J. G., M. W. van der Bijl, J. Stoof, C. M. Vandenbroucke-Grauls, and P. H. Savelkoul. 2001. Antibiotic resistance is a major risk factor for epidemic behavior of Acinetobacter baumannii. Infect Control Hosp Epidemiol 22:284-8.
Spaargaren, J., J. Stoof, H. Fennema, R. Coutinho, and P. Savelkoul. 2001. Amplified fragment length polymorphism fingerprinting for identification of a core group of Neisseria gonorrhoeae transmitters in the population attending a clinic for treatment of sexually transmitted diseases in Amsterdam, The Netherlands. J Clin Microbiol 39:2335-7.
Morre, S. A., J. M. Ossewaarde, P. H. Savelkoul, J. Stoof, C. J. Meijer, and A. J. van den Brule. 2000. Analysis of genetic heterogeneity in Chlamydia trachomatis clinical isolates of serovars D, E, and F by amplified fragment length polymorphism. J Clin Microbiol 38:3463-6.
Simoons-Smit, A. M., P. H. Savelkoul, J. Stoof, T. M. Starink, and C. M. Vandenbroucke-Grauls. 2000. Transmission of Staphylococcus aureus between humans and domestic animals in a household. Eur J Clin Microbiol Infect Dis 19:150-2.
Van Der Zwet, W. C., G. A. Parlevliet, P. H. Savelkoul, J. Stoof, A. M. Kaiser, A. M. Van Furth, and C. M. Vandenbroucke-Grauls. 2000. Outbreak of Bacillus cereus infections in a neonatal intensive care unit traced to balloons used in manual ventilation. J Clin Microbiol 38:4131-6.
van der Zwet, W. C., G. A. Parlevliet, P. H. Savelkoul, J.Stoof, A. M. Kaiser, J. G. Koeleman, and C. M. Vandenbroucke-Grauls. 1999. Nosocomial outbreak of gentamicin-resistant Klebsiella pneumoniae in a neonatal intensive care unit controlled by a change in antibiotic policy. J Hosp Infect 42:295-302.
van Doorn, N. E., F. Namavar, M. Sparrius, J. Stoof, E. P. van Rees, L. J. van Doorn, and C. M. Vandenbroucke-Grauls. 1999. Helicobacter pylori-associated gastritis in mice is host and strain specific. Infect Immun 67:3040-6.
Koeleman, J. G., J. Stoof, D. J. Biesmans, P. H. Savelkoul, and C. M. Vandenbroucke-Grauls. 1998. Comparison of amplified ribosomal DNA restriction analysis, random amplified polymorphic DNA analysis, and amplified fragment length polymorphism fingerprinting for identification of Acinetobacter genomic species and typing of Acinetobacter baumannii. J Clin Microbiol 36:2522-9.
Contact details:
Molecular Bacteriology and Immunology Group
Institute of Infection, Immunity
and Inflammation
Centre for Biomolecular Sciences
University of Nottingham
University Park
Nottingham NG7 2RD
e-mail: Jeroen.Stoof@nottingham.ac.uk
Tel: +44 (0)115 8230743