Name: Khaled Alhadyan.
Translational& Radiation Biology Research Group, Clinical Oncology, School of Medicine, City Hospital Campus, The University of Nottingham, Nottingham NG5 1PB, The United Kingdom
Papers published in peer-reviewed journals:
- Khaled Al-Hadyan, Sara Elewisy, Belal Moftah, Mohamed Shoukri, Awad Alzahrany, Ghazi Alsbeih. Establishing Cytogenetic Biodosimetry Laboratory in Saudi Arabia and Producing National Calibration Curve of Dicentric Chromosomes as Biomarker for Medical Dose Estimation in Response to Radiation Emergencies. 3Biotech Journal. (Published online, DOI 10.1007/s13205-014-0217-x).
- Ghazi Alsbeih, Medhat El-Sebaie, Nasser Al-Rajhi, Najla Al-Harbi, Khaled Al-Hadyan, Sara Al-Qahtani, Mohammad Alsubael, Mohammad Al-Shabanah,, Belal Moftah. Among 45 variants in 11 genes, HDM2 promoter polymorphisms emerge as new candidate biomarker associated with radiation toxicity. 3Biotech Journal, v.4(2); Apr 2014.
- K. Al-hadyan*, S. Binjamaan, F. Mahyoub, G. Alsbeih & B. Moftah. Development of radiation monitoring services for radiation workers in Saudi Arabia. Environmental Health Risk VII: WIT Transactions on Biomedicine and Health Book. Volume:16, 2013. Proceedings manuscript in the 7th International Conference on the Impact of Environmental Factors on Health. 23 - 25 April, 2013. Budapest, Hungary.
*Invited for oral presentation.
- Ghazi Alsbeih, Medhat El-Sebaie, Najla Al-Harbi, Khaled Al-Hadyan, Mohamed Shoukri and Nasser Al-Rajhi. SNPs in genes implicated in radiation response are associated with radiotoxicity and evoke roles as predictive and prognostic biomarkers. Radiation Oncology 8:125. May 2013.
- Al-Zahrany, Awad, Al-Hadyan, Khaled, Ahmad Nobah, Saad Aldelaijan, Venturina, L. Aubrey, Shoukri, Mohamed, Moftah, Belal and Alsbeih, Ghazi. Developing Biological Dosimetry Laboratory for the Assessment of Radiation Overexposure in Saudi Arabia. Proceedings manuscript in The 13th International Congress of the International Radiation Protection Association (IRPA).13-18 May 2012. Glasgow, United Kingdom.
- Khaled S. Al-Hadyan, Najla M. Al-harbi, Sara S. Al-qahtani, and Ghazi A. Alsbeih. Involvement of Single-Nucleotide Polymorphisms in Predisposition to Head and Neck Cancer in Saudi Arabia. Genetic Testing and Molecular Biomarkers. 16(2): 95-101, February 2012.
- G. Alsbeih, N. Al-Harbi, K. Al-Hadyan, M. El-Sebaie and N. Al-Rajhi. Association between Normal Tissue Complications after Radiotherapy and Polymorphic Variations in TGFB1 and XRCC1 Genes. Radiation Research. 173(4):505:511, 2010.
- Ghazi Alsbeih, Najla Al-Harbi, Medhat El-Sebaie, Nasser Alrajhi, Khaled Al-Hadyan and Khaled Abu-Amero. association between Mitochondrial DNA Sequence Variations and Normal Tissue Complications following Radiotherapy. Clinical Cancer Research, Dec 2009 15; 7352.
*The manuscript won the 2nd best scientific paper award of the annual report of the KFSHRC 2010.
- Ghazi Alsbeih, Medhat El-Sebaie, Najla Al-Harbi, Khaled Al-Hadyan, Muneera Al-Buhairi, Martha Torres, and Nasser Al-Rajhi. Genetic polymorphisms, protein expression and complications to radiotherapy in Saudi cancer patients. Journal of Medical Sciences, 2(2), 71-82, 2009.
*The manuscript won Hamdan Bin Rashid Al-Maktoum Award for Best Original Research Paper Published in "Journal of Medical Sciences" at its sixth term 2009-2010.
- Ghazi Atiyeh Alsbeih, Medhat Mohamed El-Sebaie, Nasser Mohamed Al-Rajhi, Najla Mohamed Al-Harbi, Khaled Saleh Al-Hadyan, Muneera Hamad Al-Buhairi, Belal Ali Moftah, Mohammad Othman Al-Shabanah and Khaled Kader Abu-Amero, Association between Xrcc1 G399A polymorphism and late complications to radiotherapy in Saudi Head & Neck cancer patients. Journal of the Egyptian National Cancer Institute,01/09/2008; 20(3):302-8.
- Alsbeih G, Al-Hadyan K. and Al-Harbi N. Assessment of the frequency of a novel MRE11 mutation responsible of the rare ataxia telangiectasia-like disorder in Saudi population. Genetic Testing. 12(3), 1-4, 2008.
- Alsbeih G, El-Sebaie M, Al-Harbi N, Al-Buhairi M, Al-Hadyan K, Al-Rajhi N. Radiosensitivity of human fibroblasts is associated with amino acid substitution variants in susceptible genes and correlates with the number of risk alleles. International Journal of Radiation Oncology, Biology and Physics. 68(1), 229-235, 2007.
- G. Alsbeih, N. Al-Harbi, M. Al-Buhairi, K. Al-Hadyan and M. Al-Hamed. Association between TP53 Codon72 Single Nucleotide Polymorphism and Radiation Sensitivity of Human Fibroblasts. Radiation Research. 167(5), 535-540, 2007. *Podcasted (see above: News in press and public media).
Molecular biology and genetics:
- DNA extraction from blood and cells in culture.
- Polymerase Chain Reaction (PCR).
- Analyze DNA sequencing results.
- Single nucleotide polymorphisms detection techniques.
- Real Time PCR Technology.
- Cytogenetic, Dicentric assay.
- Immunohistochemistry (IHC).
- Western Blotting.
- Prepare different media for cell culture.
- Initiate fibroblast cell culture from skin biopsy.
- Maintain cell lines in culture.
- Determine cellular radiosensitivity by the clonogenic survival assay.
- Sulforhodamine B (SRB) assay.
- MTT Cell Proliferation Assay.
- Estimation Reactive Oxygen Specious (RSO) level in cellular biology.
1. Conduct radiation level survey of patients and rooms.
2. Conduct contamination level survey of patients and rooms.
3. Conduct radiation level survey of radioactive packages.
Writing and communication abilities:
- Search and retrieve scientific information on the Internet.
- Capable of formulating hypothesis based on collected facts and data.
- Assist in manuscripts preparation and figures drawing.
- Skilled in graphing and statistical analysis in SigmaPlot analysis software.
- Communicate effectively with press and media addressed to general population.
PhD project title: Targeting Redox Proteins to Increase Radiotherapeutic Efficacy in Pancreatic Cancer
Summary of the project: Pancreatic cancer is the seventh leading cause of cancer death worldwide. In the United Kingdom, there were 8,773 new cases of pancreatic cancer registered in 2011 and 8,662 deaths in 2012 making it the sixth leading cause of cancer death. Between 2005 and 2009, the UK 5yr survival rate was only 3.7% with there being little change in survival rates over the last 30-40 years. Current treatment options, of chemotherapy, radiotherapy and/or surgery, have failed to improve survival - there is an urgent need for new therapies. The cellular antioxidant system regulates intracellular reactive oxygen species (ROS) in living cells to maintain redox homeostasis. Redox proteins are key members of cellular antioxidant systems, with the Thioredoxin system being an important component. This system consists of thioredoxin (Trx), thioredoxin reductase (TrxR) and thioredoxin-interacting protein (TxNIP). Studies, in breast cancer, have shown that targeting the thioredoxin system can alter the redox buffering system in cancer cells and lead to increased cancer cell sensitivity to ionising radiation. The current project will investigate the involvement of the thioredoxin system in pancreatic cancer radioresponse by using novel TrxR inhibitors (indolequinone based) developed at the University of Nottingham. Expression of Trx in pancreatic patient tumours has never previously been assessed and will be an initial focus. In vitro clonogenic experiments will be used to initially investigate drug-radiation interactions, with subsequent experiments examining the molecular basis for such interactions. Agents will subsequently be assessed for radiosenstising efficacy in breast cancer.
ALSBEIH, GHAZI, EL-SEBAIE, MEDHAT, AL-HARBI, NAJLA, AL-HADYAN, KHALED, SHOUKRI, MOHAMED and AL-RAJHI, NASSER, 2013. SNPs in genes implicated in radiation response are associated with radiotoxicity and evoke roles as predictive and prognostic biomarkers RADIATION ONCOLOGY. 8, AL-HADYAN, KHALED S., AL-HARBI, NAJLA M., AL-QAHTANI, SARA S. and ALSBEIH, GHAZI A., 2012. Involvement of Single-Nucleotide Polymorphisms in Predisposition to Head and Neck Cancer in Saudi Arabia GENETIC TESTING AND MOLECULAR BIOMARKERS. 16(2), 95-101 ALSBEIH, G., AL-HARBI, N., AL-HADYAN, K., EL-SEBAIE, M. and AL-RAJHI, N., 2010. Association between Normal Tissue Complications after Radiotherapy and Polymorphic Variations in TGFB1 and XRCC1 Genes RADIATION RESEARCH. 173(4), 505-511
ALSBEIH, GHAZI A., AL-HARBI, NAJLA M., EL-SEBAIE, MEDHAT M., AL-RAJHI, NASSER M., AL-HADYAN, KHALED S. and ABU-AMERO, KHALED K., 2009. Involvement of Mitochondrial DNA Sequence Variations and Respiratory Activity in Late Complications following Radiotherapy CLINICAL CANCER RESEARCH. 15(23), 7352-7360