Date: Thu, 6 Nov 1997 12:02:29 -0600<BR>
To: ovary-science@nottingham.ac.uk<BR>
From: "Lori E. Anderson" <leanders@students.wisc.edu><BR>
Subject: Binding affinities--PGE2/PGF2a<BR>

According to radioreceptor binding studies analyzed by Scatchard analysis
in our lab (Milo Wiltbank's), in the bovine mid-cycle corpora lutea plasma
membrane:<BR>

PGF2a Kd = 7.6 - 9.2 nM<BR>
PGE1 Kd = 11 nM<BR>

From inhibition curves, the IC50s for 4 prostaglandins also in the bovine
mid-cycle corpus luteum are as follows:<BR>

PGD2--22 nM<BR>
PGE1--14 nM<BR>
PGE2 --8 nM<BR>
PGF2a--22 nM<BR>

Be aware that both PGD2 and PGE2 appear to cross-react with the PGF2a
receptor (FP), but PGE1 does not.  Using either PGD2 or PGE2 in a tissue that
contains high levels of FP receptor, such as the CL, will confound the results
since the FP receptor may be activated.  We have little evidence for a PGD2
receptor (DP).  Preliminary experiments show that a specific DP receptor
antagonist (BW245-C) does not inhibit radioactive PGD2 binding in the
bovine CL.<BR>

See BOR Volume 52, Suppl 1, Abstract#209 for more information.<BR>

Lori E. Anderson<BR>
Research Assistant<BR>

Endocrinology--Reproductive Physiology Program<BR>
UW--Madison<BR>
1675 Observatory Drive<BR>
Madison, WI  53706<BR>
(608) 263-8802<BR>
leanders@students.wisc.edu<BR>




-- End --