1. Sections to distilled water.
2. Treat with silver solution in a coplin jar in a water bath at 45ª for 30
3 Wash in water.
4. Counterstain in 1% eosin for 30 seconds.
5. Dehydrate, clear . Mount sections in DPX
--Argentaffin,chromaffin and some lipofuscin black
To 20 mls of 10% silver nitrate add concentrated ammonia drop by drop until the
formed precipitate almost dissolves to the silver solution add 20 mls of distilled
water then filter
Waste ammoniacal silver solution is potentially explosive. Collect all
ammoniacal silver and put into the silver waste bottle - it contains saturated
sodium chloride to precipitate out the silver, this not only makes it safe but
allows the silver to be sold for reclamation. Do not pour waste solutions down
NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or
otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.
© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997
with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk