Histotechnology Technical methods

Stain for Helicobacter pylori

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GIEMSA FOR Helicobacter pylori


1. Sections to water.

2. Dilute Giemsa 1:20 with distilled water and add 2 drops of concentrated acetic acid. stain for 30 minutes

3. Wash in water.

4. Dehydrate, clear . Mount sections in DPX


--Helocobacter blue/grey

--Background pink/pale blue

--Nuclei blue



--Known Helocobacter in gastric biopsy

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk