Histotechnology Technical methods

Stain Solution

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  • Haematoxylin 6.0g
  • Aluminum Sulphate 42.0g
  • Ethylene Glycol 269ml
  • Citric Acid 1.4g
  • Sodium Iodate 0.6g
  • Distilled Water 680ml

Dissolve all the solids in the distilled water and then add the Ethylene Glycol.

NB: The haematoxylin can be dissolved in a small amount of alcohol prior to adding to the water.

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk