Histotechnology Technical methods

Stain Solution

Methods Index

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  • Haematoxylin 10g
  • Absolute Alcohol 100ml
  • Potassium Alum 200g
  • Distilled Water 2000ml
  • Mercuric Oxide 5g

Dissolve the haematoxylin in absolute alcohol in a small beaker. Dissolve the potassium alum in warm distilled water in 5L flask and bring to the boil. Remove from the heat and add the mercuric oxide and cool rapidly in a sink full of iced water. Place into a dark glass winchester.

Before use, add 0.5ml acetic acid to 60ml haematoxylin solution, then filter into a staining dish. The remainder of the solution is stored in a cool place until needed.

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk