1. Sections to distilled water.
2. Treat with 1% periodic acid for 10 minutes.
3 Wash in distilled water.
4. Place in hexamine silver solution pre-heated to 56ªc for 30-90 minutes controlling microscopically
5. Wash in distilled water.
6. Tone in 0.2% gold chloride
7. Wash in distilled water.
8. Counterstain in 1% light green or with a light haematoxylin and eosin.
9. Wash in water.
10. Dehydrate, clear . Mount sections in DPX
--Basement membrane and Mesangium black
--Background red or green
--Background colour is dependant on the counterstain used
--3% hexamine 20 mls
--5% silver nitrate 1 ml
--Mix until the precipitate disappears.
Then add to:
--5% borax (sodium tetraborate) 2 mls
--Distilled water 25 mls
THIS SOLUTION MUST BE FILTERED BEFORE USE.
Waste ammoniacal silver solution is potentially explosive. Collect all ammoniacal
silver and put into the silver waste bottle - it contains saturated sodium chloride to
precipitate out the silver, this not only makes it safe but allows the silver to be sold
for reclaimation.Do not pour waste solutions down the drain.
NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or
otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.
© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997
with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk