Histotechnology Technical methods

Stain for fibrin

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1. Sections to water.

2. Treat with iron alum for 5 minutes.

3. Wash water.

4. Stain with mayers haematoxylin for 1 minute, differentiate if necessary.

5. Blue in tap water.

6. Rinse in alcohol.

7. Stain with 0.2% orange g in alcoholic picric acid for 5 minutes

8. blot dry.

9. Stain with brilliant crystal scarlet solution for 3 minutes.

10. Wash in water.

11. Treat with phosphotungstic acid until the connective tissue appears pink/colourless approx 20-30 seconds.

12. Wash in water.

13. Stain with 1% aniline blue in 2% acetic acid for 2 seconds

14. Dehydrate, clear . Mount sections in DPX


--Nuclei blue/black

--Muscle red

--Erythrocytes red

--Collagen blue

--Fibrin red

--Early fibrin may be yellow

--Very old fibrin may be blue

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk