Histotechnology Technical methods

Stain for Fat

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1. Cut 2 frozen sections.

2. Fix in 10% formal calcium.

3. Wash in water.

4. Rinse in 60% T.E.P. (triethyl phosphate).

5. Stain in filtered oil red o for 10-15 minutes.

6. Rinse in 60% T.E.P.

7. Rinse in water.

8. Stain nuclei with Mayers haematoxylin for 1 minute. (N.B. do not differentiate)

9. Blue in tap water.

10. Mount in aqueous mountant. (glycerin jelly).


--Lipid red --Nuclei blue/black


--Not essential

--Fatty liver

--Negative control


Treat one section with a 50:50 acetone/xylene mixture for 5 minutes, wash in water then continue as from step 4.

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk