Histotechnology Technical methods

Stain for pituitary cell types

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1. Sections to water.

2. Treat with 1% periodic acid for 10 minutes.

3. Wash in water.

4. Stain in Schiffs reagent for 10 minutes.

5. Wash in tap water until sections appear pink.

6. Treat with 4% iron alum for 5 minutes.

7. Wash in distilled water.

8. Stain nuclei with Mayers haematoxylin for 5.minutes.

9. Differentiate in 1% acid alcohol.

10. Blue in tap water.

11. Stain with 0.2% orange g in alcoholic picric acid.

12. Dehydrate in absolute alcohol and examine microscopically.

13. Differentiate in 70% alcohol until the background appears colourless.

14. Dehydrate, clear . Mount sections in DPX


--Basophil cells magenta

--Acidophil cells yellow

--Red blood cells yellow

--Nuclei blue/black

--Chromophobes pale blue/grey


Schiffs reagent

--see "Periodic Acid, Schiff"

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk