1. Sections to water.
2. Wash in distilled water.
3. Stain in buffered toluidine blue solution for 20 minutes.
4. Wash well in water.
5. Dehydrate, clear . Mount sections in DPX
TOLUIDINE BLUE IN pH 6.8 PHOSPHATE BUFFER
--Phosphate buffer pH 6.8 50ml
--1% aqueous toluidine blue 1ml
Gastric biopsy known to contain H. Pylori
NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or
otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.
© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997
with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk