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Stain for calcium

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1. Sections to water.

2. Place sections in 1% silver nitrate solution under ultra- violet light for 45 minutes.

3. Wash in distilled water.

4. Treat with 3% sodium thiosulphate for 5 minutes.

5. Wash in water.

6. Counterstain with van Gieson for 5 minutes.

7. Wash in alcohol.

8. Clear . Mount sections in DPX


Mineralised bone black Osteoid red


VAN GIESON --1% acid fuchsin 7.5 mls

--Saturated picric acid 92.5 mls

--Distilled water 100 mls

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk