Histotechnology Technical methods

Stain for myelin

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NB Paraffin sections need to be cut at 10-15µ for this technique.

1. Bring sections to distilled water.

2. Place sections in stain for 1 hour at room temperature (coplin jar).

3. Wash well in tap water.

4. Differentiate in 4% iron alum just long enough to distinguish the grey matter or the degenerated areas.

5. Wash well in several changes of distilled water.

6. Complete differentiation (clear up background) with Weigert's borax ferricyanide solution.

7. Wash well in several changes of distilled water, followed by tap water.

8. Dehydrate, clear . Mount sections in DPX


--Myelin - black

--Background - clear or yellow


Staining Solution

--4% iron alum 300ml

--1% haematoxylin 300ml Weigert's borax ferricyanide differentiator

--Borax 2g

--Potassium ferricyanide 2.5g

--Distilled water 200ml

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk