Associate Professor in Allergy and Infectious Diseases, Faculty of Science
Dr Franco Falcone received a Degree in Biochemistry (Dipl.-Biochem.) from the University of Tübingen in 1991, and a Degree in Biology (Dipl.-Biol.), also from the University of Tübingen in 1993.
He then moved further North to work with Dr Helmut Haas and received a PhD (Dr. rer. nat.) in Immunology from the University of Lübeck in 1996. The PhD thesis received two scientific awards (Best Doctoral Thesis of the Year 2006). After working as a Postdoctoral Research Fellow in the Research Centre Borstel, Germany, he obtained a Fellowship from the Deutsche Forschungsgemeinschaft (DFG) which took him to Edinburgh University (Prof. R. M. Maizels, Prof. A. G. Rossi and Prof. J. E. Allen). He worked as a Scientific Manager of the Association of Clinical Research Centers in Münster, Germany (2001-2003), before returning to a research career with a Wellcome Trust Research Career Development Fellowship which took him to Nottingham, where he has been working since.
Dr Franco H. Falcone is lead and convener of the 3rd year module B33VPI: Viral and Parasitic Infection which encompasses Virology (HIV, Hepatitis, Influenza), protozoan (malaria, giardiasis,… read more
Our laboratory has several lines of research interests. I have been involved in human basophil research for the past two decades. I have worked on optimizing protocols for the purification of this… read more
METCALFE DD, PAWANKAR R, ACKERMAN SJ, AKIN C, CLAYTON F, FALCONE FH, GLEICH GJ, IRANI AM, JOHANSSON MW, KLION AD, LEIFERMAN KM, LEVI-SCHAFFER F, NILSSON G, OKAYAMA Y, PRUSSIN C, SCHROEDER JT, SCHWARTZ LB, SIMON HU, WALLS AF and TRIGGIANI M, 2016. Biomarkers of the involvement of mast cells, basophils and eosinophils in asthma and allergic diseases. The World Allergy Organization journal. 9, 7
HAGE N, HOWARD T, PHILLIPS C, BRASSINGTON C, OVERMAN R, DEBRECZENI J, GELLERT P, STOLNIK S, WINKLER GS and FALCONE FH, 2015. Structural basis of Lewis(b) antigen binding by the Helicobacter pylori adhesin BabA. Science Advances. 1(7), e1500315
Dr Nora Francini (EPSRC Fellow, co-supervised with Dr K Robinson)
- Mohammed Alissa (Postgraduate Student)
- Fatou Gai (Postgraduate Student, funded by the Islamic Development Bank, co-supervised with Prof M Doenhoff)
- Abdulaziz Alouffi (Postgraduate Student, Saudi Government funded)
- Eman Ali (Postgraduate Student, Vice Chancellor's Scholarship for Research Excellence (International) funded)
- Waleed Alharbi (Postgraduate Student),
- Nafal Al-Barwary (Postgraduate Student, funded by the Government of Kurdistan)
- Aymen Salih Muhammad (Postgraduate Student)
- Fahad Almughem (Postgraduate Student)
- Zainab Fathi (Postgraduate Student)
- Vasiliki Paraskevopoulou (Postgraduate Student)
- Rachel Hampson
- Lauren Sheldon
ERASMUS Plus students
- Veronica Garcia (ErasmusPLUS student)
- Irene Romero (Erasmus PLUS student)
Shaun Morroll (2003-2006)
Driton Vllasaliu (2011-2012), Co-supervised with Snow Stolnik and Martin Garnett), now Senior Lecturer, University of Lincoln, UK
Fernanda Ludolf-Ribeiro, Rene Rachou Research Center/CPqRR -- Oswaldo Cruz Foundation in the State of Minas Gerais, Brazil (2011) lab visit funded by Boehringer Ingelheim Fonds and ESCMID/ISID)
- Naim Hage (PhD student, EPSRC AstraZeneca Doctoral Training Centre in Targeted Therapeutics funded, now Associate Consultant at Quintiles in London)
- Daniel Wan (BBSRC-funded PhD-student, now postdoc at the Allergy and Immunology Institute in La Jolla, California)
- Walla Al-Elwani (PhD student)
- Muhammad Zeeshan Danish
- Andrew J Hall (PhD student, now Development Scientist (Cell Bioprocess) at GE Healthcare)
- Ishwinder Kaur (PhD student, now Research Fellow at the School of Pharmacy, University of Nottingham)
- Gerard Byrne (PhD student, now Senior scientist at Merck Sharp & Dohme)
- Jennie Lord (PhD student, now National Centre Manager at the EPSRC Centre for Innovative Manufacturing in Food)
- Shaun Morroll (2003-2006; Wellcome Trust-funded Postdoctoral research Associate)
Aisha Sahi (2015), Lucy Clasper (2014), Nafsika Sismanoglou (2014), Eman Ali (2013), Victoria Stavrou (2013), Sam Sherratt (2013), Tom Chesterton (2013), Daniel Alanine (2012), Emanuele Mura (2012), Owen Stretton (2011), Mark Sven James-Bhasin (2011), Paul Cato(2010), Ian Garner (2011), Henrike Moeller (2011), Xianxian Zou (2009), Chris Todd (2007), Sharon Turner (2006), Kerstin Papenfuss (2006).
to be completed
Dr Franco H. Falcone is lead and convener of the 3rd year module B33VPI: Viral and Parasitic Infection which encompasses Virology (HIV, Hepatitis, Influenza), protozoan (malaria, giardiasis, trichomoniasis) as well as metazoan infections (helminthiasis) and exoparasites.
He has been previously involved in teaching Pharmaceutical Microbiology (B32CO2 and B32C08) as well as Sterile Production (B33F06, B33E11).
He also runs regular workshops on PCR, Western Blotting, general lab skills and research ethics for postgraduate students.
Our laboratory has several lines of research interests. I have been involved in human basophil research for the past two decades. I have worked on optimizing protocols for the purification of this very rare leukocyte, and studied mechanisms of alternative (non-classical) basophil activation, some of which are IgE-dependent, but do not dependent on IgE specificity. I am interested in understanding the role of basophils in innate immunity, immunity to parasites and allergy. More recently, I have been involved as a collaborator in the development of a diagnostic device which combines the analytical power of protein arrays with the biological specificity and sensitivity of live basophils. For this line of work we have generated our own humanised RBL reporter system (NFAT-DsRed) which is available for non-commercial reasons via an MTA.
Another line of research, which is more recent and goes back to 2003, involves the study of human chitinases and the sensing of chitin by the innate immune system. We are also interested in elucidating the roles of chitinases in humans and their interplay with pathogen-derived chitin.
More recently we have developed an interest in understanding and exploiting the adhesion mechanisms of Salmonella and Helicobacter to intestinal and gastric epithelium, respectively. Most recently we have published the three dimensional structure of BabA, the best studied adhesin of Helicobacter pylori.
Memberships of Committees and Professional Bodies
- Member of the British Society for Immunology (BSI)
- Member of the German Society for Parasitology (DGP)
- Member of the European Histamine Research Society (EHRS)
- Founding member of the European Mast Cell and Basophil Research Network (EMBRN)
- Member of the British Society for Allergy and Clinical Immunology (BSACI)
METCALFE DD, PAWANKAR R, ACKERMAN SJ, AKIN C, CLAYTON F, FALCONE FH, GLEICH GJ, IRANI AM, JOHANSSON MW, KLION AD, LEIFERMAN KM, LEVI-SCHAFFER F, NILSSON G, OKAYAMA Y, PRUSSIN C, SCHROEDER JT, SCHWARTZ LB, SIMON HU, WALLS AF and TRIGGIANI M, 2016. Biomarkers of the involvement of mast cells, basophils and eosinophils in asthma and allergic diseases. The World Allergy Organization journal. 9, 7 HAGE N, HOWARD T, PHILLIPS C, BRASSINGTON C, OVERMAN R, DEBRECZENI J, GELLERT P, STOLNIK S, WINKLER GS and FALCONE FH, 2015. Structural basis of Lewis(b) antigen binding by the Helicobacter pylori adhesin BabA. Science Advances. 1(7), e1500315 GILICZE AB, WIENER Z, TÓTH S, BUZÁS E, PÁLLINGER E, FALCONE FH and FALUS A, 2014. Myeloid-Derived microRNAs, miR-223, miR27a, and miR-652, Are Dominant Players in Myeloid Regulation. BioMed research international. 2014, 870267 SIEBENHAAR F, FALCONE FH, TILIGADA E, HAMMEL I, MAURER M, SAGI-EISENBERG R and LEVI-SCHAFFER F, 2014. The search for Mast Cell and Basophil models - Are we getting closer to pathophysiological relevance? Allergy. 70(1), 1-5 FALCONE FH and GIBBS BF, 2014. Purification of basophils from peripheral human blood. Methods in molecular biology (Clifton, N.J.). 1192, 35-47 SABATO V, BOITA M, SHUBBER S, BRIDTS CH, SHIBUYA A, DE CLERCK LS, FALCONE FH and EBO DG, 2014. Mechanism of phosphatidylserine inhibition of IgE/FcεRI-dependent anaphylactic human basophil degranulation via CD300a. The Journal of allergy and clinical immunology. 134(3), 734-737.e3 LUDOLF F, PATROCÍNIO PR, CORRÊA-OLIVEIRA R, GAZZINELLI A, FALCONE FH, TEIXEIRA-FERREIRA A, PERALES J, OLIVEIRA GC and SILVA-PEREIRA RA, 2014. Serological screening of the Schistosoma mansoni adult worm proteome. PLoS neglected tropical diseases. 8(3), e2745 WAN D, LUDOLF F, ALANINE DGW, STRETTON O, ALI ALI E, AL-BARWARY N, WANG X, DOENHOFF MJ, MARI A, FITZSIMMONS CM, DUNNE DW, NAKAMURA R, OLIVEIRA GC, ALCOCER MJC and FALCONE FH, 2014. Use of humanised rat basophilic leukaemia cell line RS-ATL8 for the assessment of allergenicity of Schistosoma mansoni proteins. PLoS neglected tropical diseases. 8(9), e3124
GIBBS BF and FALCONE FH, 2014. Preface. Basophils and mast cells. Methods in molecular biology (Clifton, N.J.). 1192, v-vi
MANNO N, SHERRATT S, BOARETTO F, COICO FM, CAMUS CE, CAMPOS CJ, MUSUMECI S, BATTISTI A, QUINNELL RJ, LEÓN JM, VAZZA G, MOSTACCIUOLO ML, PAOLETTI MG and FALCONE FH, 2014. High prevalence of chitotriosidase deficiency in Peruvian Amerindians exposed to chitin-bearing food and enteroparasites. Carbohydrate polymers. 113, 607-14 SABATO, VITO, BOITA, MONICA, SHUBBER, SAIF, BRIDTS, CHRIS H., SHIBUYA, AKIRA, DE CLERCK, LUC S., FALCONE, FRANCO H. and EBO, DIDIER G., 2014. Mechanism of phosphatidylserine inhibition of IgE/Fc epsilon RI-dependent anaphylactic human basophil degranulation via CD300a JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. 134(3), 734-737 NULLENS S, SABATO V, FABER M, LEYSEN J, BRIDTS CH, DE CLERCK LS, FALCONE FH, MAURER M and EBO DG, 2013. Basophilic histamine content and release during venom immunotherapy: Insights by flow cytometry. Cytometry. Part B, Clinical cytometry.
FOWLER R, VLLASALIU D, FALCONE FH, GARNETT M, SMITH B, HORSLEY H, ALEXANDER C and STOLNIK S, 2013. Uptake and transport of B12-conjugated nanoparticles in airway epithelium. Journal of controlled release : official journal of the Controlled Release Society. 172(1), 374-381 WULFERT, F., SANYASI, G., TONGEN, L., WATANABE, L.A., WANG, X., RENAULT, N.K., FALCONE, F.H., JACOB, C.M.A. and ALCOCER, M.J.C., 2012. Prediction of tolerance in children with IgE mediated cow's milk allergy by microarray profiling and chemometric approach Journal of Immunological Methods. 382(1-2), 48–57 FÖRSTER A, FALCONE FH, GIBBS BF, PREUSSNER LM, FIEBIG BS, ALTUNOK H, SEEGER JM, CERNY-REITERER S, RABENHORST A, PAPENFUSS K, VALENT P, KASHKAR H and HARTMANN K, 2012. Anti-fas/cd95 and tumor necrosis factor-related apoptosis-inducing ligand (trail) differentially regulate apoptosis in normal and neoplastic human basophils. Leukemia & lymphoma.
KAUR, I., SCHRAMM, G., EVERTS, B., SCHOLZEN, T., KINDLE, K.B., BEETZ, C., MONTIEL-DUARTE, C., BLINDOW, S., JONES, A.T., HAAS, H., STOLNIK, S., HEERY, D.M. and FALCONE, F.H., 2011. Interleukin-4 inducing principle from Schistosoma mansoni eggs contains a functional C-terminal nuclear localization signal necessary for nuclear translocation in mammalian cells but not for its uptake Infection and Immunity. 79(4), 1779-1788 RENAULT, N K, GADDIPATI, S R, WULFERT, F, FALCONE, F H, MIROTTI, L, TIGHE, P J, WRIGHT, V and ALCOCER, M J C, 2011. Multiple protein extract microarray for profiling human food-specific immunoglobulins A, M, G and E. Journal of Immunological Methods. 364(1-2), 21-32 FALCONE, F H, KNOL, E F and GIBBS, B F, 2011. The role of basophils in the pathogenesis of allergic disease. Clinical and Experimental Allergy. 41(7), 939-47 GENTINETTA, T., PECARIC-PETKOVIC, T., WAN, D., FALCONE, F.H., DAHINDEN, C.A., PICHLER, W.J. and HAUSMANN, O.V., 2011. Individual IL-3 priming is crucial for consistent in vitro activation of donor basophils in patients with chronic urticaria Journal of Allergy and Clinical Immunology. 128(6), 1227-1234.e5 FEARY, J.R., VENN, A.J., MORTIMER, K., BROWN, A.P., HOOI, D., FALCONE, F.H., PRITCHARD, D.I. and BRITTON, J.R., 2010. Experimental hookworm infection: a randomized placebo-controlled trial in asthma Clinical & Experimental Allergy. 40(2), 299-306 FEARY, J., VENN, A., BROWN, A., HOOI, D., FALCONE, F.H., MORTIMER, K., PRITCHARD, D.I. and BRITTON, J., 2009. Safety of hookworm infection in individuals with measurable airway responsiveness: a randomized placebo-controlled feasibility study Clinical & Experimental Allergy. 39(7), 1060-1068 FALCONE, F.H., TELFORD, G., HOOI, D., BROWN, A.P., SEABRA, R., FEARY, J., VENN, A., BRITTON, J. and PRITCHARD, D.I., 2009. Antigen-driven basophil activation is indicative of early Necator americanus infection in IgE-seronegative patients Journal of Allergy and Clinical Immunology. 124(6), 1343-1350.e7
FALCONE, F., LIN, J., RENAULT, N., HAAS, H., SCHRAMM, G., BERNARD, F. and ALCOCER, M.J.C., 2009. The Live Basophil Allergen Array (LBAA): A Pilot Study. In: FALUS, A., ed., Immunomics Reviews 2. Springer. 153-169
WANG, N., ADAMS, G., BUTTERY, L., FALCONE, F.H. and STOLNIK, S., 2009. Alginate encapsulation technology supports embryonic stem cells differentiation into insulin-producing cells Journal of Biotechnology. 144(4), 304-312 GIBBS BF, STREATFIELD C and FALCONE FH, 2009. Basophils As Critical Orchestrators Of Th2-Type Immune Responses Expert Rev Clin Immunol. 5(6), 725-734 HALL, ANDREW J, MORROLL, SHAUN, TIGHE, PATRICK, GÖTZ, FRIEDRICH and FALCONE, FRANCO H, 2008. Human chitotriosidase is expressed in the eye and lacrimal gland and has an antimicrobial spectrum different from lysozyme. Microbes and Infection. 10(1), 69-78 BYRNE, G.D., PITTER, M.C., ZHANG, J., FALCONE, F.H., STOLNIK, S. and SOMEKH, M.G., 2008. Total internal reflection microscopy for live imaging of cellular uptake of sub-micron non-fluorescent particles Journal of Microscopy. 231(1), 168-179 GIBBS, B F, PAPENFUSS, K and FALCONE, F H, 2008. A comparison of procedures for purifying human basophils to near homogeneity. Inflammation Research. 57 Suppl 1, S11-2 LIN, J., RENAULT, N., HAAS, H., SCHRAMM, G., VIETHS, S., VOGEL, L., FALCONE, F.H. and ALCOCER, M.J.C., 2007. A novel tool for the detection of allergic sensitization combining protein microarrays with human basophils. Clinical & Experimental Allergy Blackwell Synergy. Available at: <http://www.blackwell-synergy.com/loi/cea>
LIN, J, RENAULT, N, HAAS, H, SCHRAMM, G, VIETHS, S, VOGEL, L, FALCONE, F H and ALCOCER, M J C, 2007. A novel tool for the detection of allergic sensitization combining protein microarrays with human basophils. Clinical and experimental allergy: Journal of the British Society for Allergy and Clinical Immunology. 37(12), 1854-62
HALL, A.J., QUINNELL, R.J., RAIKO, A., LAGOG, M., SIBA, P., MORROLL, S. and FALCONE, F.H., 2007. Allelic frequency of the 24bp mutation in human chitotriosidase (CHIT1) in a population from a hookworm-endemic region of Papua New Guinea Infection Genetics & Evolution. 7, 743-747
HALL AJ, QUINNELL RJ, RAIKO A, LAGOG M, SIBA P, MORROLL S and FALCONE FH, 2007. Chitotriosidase deficiency is not associated with human hookworm infection in a Papua New Guinean population. Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases. 7(6), 743-7 FALCONE, F.H. and PRITCHARD, D.I., 2005. Parasite role reversal: Worms on trial Trends in Parasitology. 21(4), 157-160 FALCONE, F H, MORROLL, S and GIBBS, B F, 2005. Lack of protease activated receptor (PAR) expression in purified human basophils. Inflammation Research. 54 Suppl 1, S13-4 FALCONE, FRANCO H, LOUKAS, ALEX, QUINNELL, RUPERT J and PRITCHARD, DAVID I, 2004. The innate allergenicity of helminth parasites. Clinical Reviews in Allergy and Immunology. 26(1), 61-72 FALCONE,F.H., 2004. Basophils and immunity to parasites: an update Revue Francaise d'Allergologie et Immunologie Clinique. 44(1), 14-22 SCHRAMM, G., FALCONE, F.H., GRONOW, A., HAISCH, K., MAMAT, U., DOENHOFF, M.J., OLIVEIRA, G., GALLE, J., DAHINDEN, C.A. and HAAS, H., 2003. Molecular characterization of an interleukin-4-inducing factor from Schistosoma mansoni eggs. Journal of Biological Chemistry. 278(20), 18384-18392 FALCONE, F.H., ROSSI, A.G., SHARKEY, R., BROWN, A.P., PRITCHARD, D.I. and MAIZELS, R.M., 2001. Ascaris suum-derived products induce human neutrophil activation via a G protein-coupled receptor that interacts with the interleukin-8 receptor pathway Infection and Immunity. 69(6), 4007-4018 FALCONE, F H, PRITCHARD, D I and GIBBS, B F, 2001. Do basophils play a role in immunity against parasites? Trends in Parasitology. 17(3), 126-9 FALCONE, F.H., LOKE, P., ZANG, X., MACDONALD, A.S., MAIZELS, R.M. and ALLEN, J.E., 2001. A Brugia malayi homolog of macrophage migration inhibitory factor reveals an important link between macrophages and eosinophil recruitment during nematode infection The Journal of Immunology. 167(9), 5348-5354 HAISCH, K., SCHRAMM, G., FALCONE, F.H., ALEXANDER, C., SCHLAAK, M. and HAAS, H., 2001. A glycoprotein from Schistosoma mansoni eggs binds non-antigen-specific immunoglobulin E and releases interleukin-4 from human basophils Parasite Immunology. 23(8), 427-434
FALCONE, F H, HAAS, H and GIBBS, B F, 2000. The human basophil: a new appreciation of its role in immune responses. Blood. 96(13), 4028-38
FALCONE, F H, TETTEH, K K, HUNT, P, BLAXTER, M L, LOUKAS, A and MAIZELS, R M, 2000. The new subfamily of cathepsin-Z-like protease genes includes Tc-cpz-1, a cysteine protease gene expressed in Toxocara canis adults and infective stage larvae. Experimental Parasitology. 94(3), 201-7 MAIR, G, SHI, H, LI, H, DJIKENG, A, AVILES, H O, BISHOP, J R, FALCONE, F H, GAVRILESCU, C, MONTGOMERY, J L, SANTORI, M I, STERN, L S, WANG, Z, ULLU, E and TSCHUDI, C, 2000. A new twist in trypanosome RNA metabolism: cis-splicing of pre-mRNA. RNA. 6(2), 163-9 MAIZELS, R M, HOLLAND, M J, FALCONE, F H, ZANG, X X and YAZDANBAKHSH, M, 1999. Vaccination against helminth parasites--the ultimate challenge for vaccinologists? Immunological reviews. 171, 125-47 HAAS, H., FALCONE, F.H., SCHRAMM, G., HAISCH, K., GIBBS, B.F., KLAUCKE, J., POPPELMANN, M., BECKER, W.M., GABIUS, H.J. and SCHLAAK, M., 1999. Dietary lectins can induce in vitro release of IL-4 and IL-13 from human basophils Eur. J. Immunol. 29, 918-927
HAAS, H., FALCONE, F.H., HOLLAND, M.J., SCHRAMM, G., HAISCH, K., GIBBS, B.F., BUFE, A. and SCHLAAK, M., 1999. Early interleukin-4: Its role in the switch towards a Th2 response and lgE-mediated allergy. Int. Arch. Allergy Immunol.. 119, 86-94
HAAS H, FALCONE FH, HOLLAND MJ, SCHRAMM G, HAISCH K, GIBBS BF, BUFE A and SCHLAAK M, 1999. Early interleukin-4: its role in the switch towards a Th2 response and IgE-mediated allergy. International archives of allergy and immunology. 119(2), 86-94 GIBBS, B.F., NOLL, T., FALCONE, F.H., HAAS, H., VOLLMER, E., VOLLRATH, I., WOLFF, H.H. and AMON, U., 1997. A three-step procedure for the purification of human basophils from buffy coat blood. Inflammation Research. 46, 137-142
GIBBS, B F, HAAS, H, FALCONE, F H, ALBRECHT, C, VOLLRATH, I B, NOLL, T, WOLFF, H H and AMON, U, 1996. Purified human peripheral blood basophils release interleukin-13 and preformed interleukin-4 following immunological activation. European Journal of Immunology. 26(10), 2493-8 FALCONE, F H, DAHINDEN, C A, GIBBS, B F, NOLL, T, AMON, U, HEBESTREIT, H, ABRAHAMSEN, O, KLAUCKE, J, SCHLAAK, M and HAAS, H, 1996. Human basophils release interleukin-4 after stimulation with Schistosoma mansoni egg antigen. European Journal of Immunology. 26(5), 1147-55
FALCONE, F H, ZAHNER, H, SCHLAAK, M and HAAS, H, 1995. In vitro cultivation of third-stage larvae of Brugia malayi to the young adult stage. Tropical Medicine and Parasitology. 46(4), 230-4
FALCONE, F H, SCHLAAK, M and HAAS, H, 1995. In vitro cultivation of Brugia malayi, a parasitic nematode that causes human lymphatic filariasis ALTEX. 12(4), 179-187