Linhua Pang

Contact

• workRoom B26 Clinical Sciences Building
  Nottingham City Hospital
  Hucknall Road
  Nottingham
  NG5 1PB
  UK
• work0115 82 31716
• fax0115 82 31946
• linhua.pang@nottingham.ac.uk

Expertise Summary

Synthetic functions of airway structural cells (airway smooth muscle cells, fibroblasts, epithelial cells), particularly the expression and production of inflammatory cytokines, chemokines, growth factors, angiogenic factors, inflammatory enzymes and lipid mediators, and their role in the pathophysiology of asthma, idiopathic pulmonary fibrosis, COPD and cyctic fibrosis.

• Molecular mechanisms of transcriptional regulation of key genes in human airway structural cells, particularly epigenetic mechanisms, including transcription factor activation and promoter binding, histone modifications, promoter DNA methylation, epigenetic modifying enzyme activation and recruitment, and interactions between different epigenetic pathways.
• Molecular mechanism of action of drugs that target key gene expression and their therapeutic potential in in vitro cell model of respiratory diseases, providing the basis for further translational studies.

Teaching Summary

Airway inflammation and remodelling in chronic respiratory diseases.

Anti-inflammatory drug effect and molecular mechanisms.

Gene expression and regulation in respiratory structural cells.

Research Summary

I am a non-clinical Associate Professor and Reader in Respiratory Medicine at the University of Nottingham based at Nottingham City Hospital. My main research interests are the role of airway… read more

Recent Publications

• TATLER AL, JOHN AE, JOLLY L, HABGOOD A, PORTE J, BRIGHTLING C, KNOX AJ, PANG L, SHEPPARD D, HUANG X and JENKINS G, 2011. Integrin Αvβ5-Mediated Tgf-Β Activation By Airway Smooth Muscle Cells In Asthma. Journal Of Immunology (Baltimore, Md. : 1950). 187(11), 6094-107
• COWARD WR, WATTS K, FEGHALI-BOSTWICK CA, JENKINS G and PANG L, 2010. Repression Of Ip-10 By Interactions Between Histone Deacetylation And Hypermethylation In Idiopathic Pulmonary Fibrosis. Molecular And Cellular Biology. 30(12), 2874-86
• CLARKE DL, CLIFFORD RL, JINDARAT S, PROUD D, PANG L, BELVISI M and KNOX AJ, 2010. Tnfα And Ifnγ Synergistically Enhance Transcriptional Activation Of Cxcl10 In Human Airway Smooth Muscle Cells Via Stat-1, Nf-Κb, And The Transcriptional Coactivator Creb-Binding Protein. The Journal Of Biological Chemistry. 285(38), 29101-10
• JOHN, ALISON E, ZHU, YONG M, BRIGHTLING, CHRISTOPHER E, PANG, LINHUA and KNOX, ALAN J, 2009. Human airway smooth muscle cells from asthmatic individuals have CXCL8 hypersecretion due to increased NF-kappa B p65, C/EBP beta, and RNA polymerase II binding to the CXCL8 promoter. Journal of immunology (Baltimore, Md. : 1950). 183(7), 4682-92

• View all publications

Current Research

I am a non-clinical Associate Professor and Reader in Respiratory Medicine at the University of Nottingham based at Nottingham City Hospital. My main research interests are the role of airway structural cells in airway inflammation, repair, and structural remodelling in the context of chronic airway diseases such as asthma, idiopathic pulmonary fibrosis, COPD and cyctic fibrosis. I am particularly interested in the expression and molecular mechanisms of regulation of genes that are important in the pathophysiology of the lung diseases. I am also interested in the effects, mechanisms of action and therapeutic potential of drugs that target these genes. I have published over 35 articles in scientific journals and am currently on the editorial board of the American Journal of Respiratory Cell and Molecular Biology. Our work is supported by research grants from a number of sources including Medical Research Council, the Wellcome Trust, Btitish Lung Foundation and Asthma UK.

Future Research

Airway structural cell responses to quorum sensing molecules in airway inflammation and infection in cystic fibrosis and COPD.

• TATLER AL, JOHN AE, JOLLY L, HABGOOD A, PORTE J, BRIGHTLING C, KNOX AJ, PANG L, SHEPPARD D, HUANG X and JENKINS G, 2011. Integrin Αvβ5-Mediated Tgf-Β Activation By Airway Smooth Muscle Cells In Asthma. Journal Of Immunology (Baltimore, Md. : 1950). 187(11), 6094-107
• COWARD WR, WATTS K, FEGHALI-BOSTWICK CA, JENKINS G and PANG L, 2010. Repression Of Ip-10 By Interactions Between Histone Deacetylation And Hypermethylation In Idiopathic Pulmonary Fibrosis. Molecular And Cellular Biology. 30(12), 2874-86
• CLARKE DL, CLIFFORD RL, JINDARAT S, PROUD D, PANG L, BELVISI M and KNOX AJ, 2010. Tnfα And Ifnγ Synergistically Enhance Transcriptional Activation Of Cxcl10 In Human Airway Smooth Muscle Cells Via Stat-1, Nf-Κb, And The Transcriptional Coactivator Creb-Binding Protein. The Journal Of Biological Chemistry. 285(38), 29101-10
• JOHN, ALISON E, ZHU, YONG M, BRIGHTLING, CHRISTOPHER E, PANG, LINHUA and KNOX, ALAN J, 2009. Human airway smooth muscle cells from asthmatic individuals have CXCL8 hypersecretion due to increased NF-kappa B p65, C/EBP beta, and RNA polymerase II binding to the CXCL8 promoter. Journal of immunology (Baltimore, Md. : 1950). 183(7), 4682-92
• COWARD,, WATTS,, FEGHALI-BOSTWICK,, KNOX, and PANG,, 2009. Defective Histone Acetylation Is Responsible for the Diminished Expression of Cyclooxygenase-2 in Idiopathic Pulmonary Fibrosis. Molecular and cellular biology.
• MULLAN, CHARLOTTE S, RILEY, MICHAEL, CLARKE, DEBORAH, TATLER, AMANDA, SUTCLIFFE, AMY, KNOX, ALAN J and PANG, LINHUA, 2008. Beta-tryptase regulates IL-8 expression in airway smooth muscle cells by a PAR-2-independent mechanism. American journal of respiratory cell and molecular biology. 38(5), 600-8
• TATLER, AMANDA L, PORTE, JOANNE, KNOX, ALAN, JENKINS, GISLI and PANG, LINHUA, 2008. Tryptase activates TGFbeta in human airway smooth muscle cells via direct proteolysis. Biochemical and biophysical research communications. 370(2), 239-42
• PANG, L., NIE, M., CORBETT, L., SUTCLIFFE, A. and KNOX, A.J., 2006. Mast cell β-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities The Journal of Immunology. 176(6), 3788-3795
• CLAYTON, A., HOLLAND, E., PANG, L. and KNOX, A., 2005. Interleukin-1β differentially regulates β₂ adrenoreceptor and prostaglandin E₂-mediated cAMP accumulation and chloride efflux from Calu-3 bronchial epithelial cells: Role of receptor changes, adenylyl cyclase, cyclo-oxygenase 2, and protein kinase A Journal of Biological Chemistry. 280(25), 23451-23463
• NIE, M., CORBETT, L., KNOX, A.J. and PANG, L., 2005. Differential regulation of chemokine expression by peroxisome proliferator-activated receptor γ agonists: interactions with glucocorticoids and β₂-agonists Journal of Biological Chemistry. 280(4), 2550-2561
• NIE, M., KNOX, A.J. and PANG, L., 2005. β₂-Adrenoceptor agonists, like glucocorticoids, repress eotaxin gene transcription by selective inhibition of histone H4 acetylation The Journal of Immunology. 175(1), 478-486
• STOCKS, JOANNE, BRADBURY, DAWN, CORBETT, LISA, PANG, LINHUA and KNOX, ALAN J, 2005. Cytokines upregulate vascular endothelial growth factor secretion by human airway smooth muscle cells: Role of endogenous prostanoids. FEBS Letters. 579(12), 2551-6
• PETKOVA, D K, CLELLAND, C, RONAN, J, PANG, L, COULSON, J M, LEWIS, S and KNOX, A J, 2004. Overexpression of cyclooxygenase-2 in non-small cell lung cancer. Respiratory Medicine. 98(2), 164-72
• ZHU, Y.M., BRADBURY, D.A., PANG, L. and KNOX, A.J., 2003. Transcriptional regulation of interleukin (IL)-8 by bradykinin in human airway smooth muscle cells involves prostanoid-dependent activation of AP-1 and nuclear factor (NF)-IL-6 and prostanoid-independent activation of NF-κB Journal of Biological Chemistry. 278(31), 29366-29375
• NIE, M., PANG, L., INOUE, H. and KNOX, A.J., 2003. Transcriptional regulation of cyclooxygenase 2 by bradykinin and interleukin-1β in human airway smooth muscle cells: involvement of different promoter elements, transcription factors, and histone H4 acetylation Molecular and Cellular Biology. 23(24), 9233-9244
• PANG, LINHUA, NIE, MEI, CORBETT, LISA and KNOX, ALAN J, 2003. Cyclooxygenase-2 expression by nonsteroidal anti-inflammatory drugs in human airway smooth muscle cells: role of peroxisome proliferator-activated receptors. The Journal of Immunology. 170(2), 1043-51
• SáNCHEZ-ALCáZAR, JOSé A, BRADBURY, DAWN A, PANG, LINHUA and KNOX, ALAN J, 2003. Cyclooxygenase (COX) inhibitors induce apoptosis in non-small cell lung cancer through cyclooxygenase independent pathways. Lung Cancer. 40(1), 33-44
• PANG, L., NIE, M., CORBETT, L., DONNELLY, R., GRAY, S. and KNOX, A.J., 2002. Protein kinase C-ε mediates bradykinin-induced cyclooxygenase-2 expression in human airway smooth muscle cells FASEB Journal. 16(11), 1435-1437
• BRADBURY, D A, NEWTON, R, ZHU, Y-M, STOCKS, J, CORBETT, L, HOLLAND, E D, PANG, L H and KNOX, A J, 2002. Effect of bradykinin, TGF-beta1, IL-1beta, and hypoxia on COX-2 expression in pulmonary artery smooth muscle cells. American Journal of Physiology: Lung Cellular and Molecular Physiology. 283(4), L717-25
• RODGERS, HELEN C, PANG, LINHUA, HOLLAND, ELAINE, CORBETT, LISA, RANGE, SIMON and KNOX, ALAN J, 2002. Bradykinin increases IL-8 generation in airway epithelial cells via COX-2-derived prostanoids. American Journal of Physiology: Lung Cellular and Molecular Physiology. 283(3), L612-8
• KNOX, ALAN J and PANG, LINHUA, 2002. Glucocorticoid and beta-adrenoceptor agonist interactions in asthma. Lancet. 360(9342), 1265-6
• KNOX, A.J., CORBETT, L., STOCKS, J., HOLLAND, E., ZHU, Y.M. and PANG, L., 2001. Human airway smooth muscle cells secrete vascular endothelial growth factor: up-regulation by bradykinin via a protein kinase C and prostanoid-dependent mechanism The FASEB Journal: official publication of the Federation of American Societies for Experimental Biology. 15(13), 2480-2488
• PANG, L. and KNOX, A.J., 2001. Regulation of TNF-α-induced eotaxin release from cultured human airway smooth muscle cells by β₂-agonists and corticosteroids FASEB Journal. 15(1), 261-269
• KNOX, A J, ZHU, Y M and PANG, L, 2001. Do long-acting beta2-adrenoceptor agonists enhance the anti-inflammatory effect of glucocorticoids in asthma? European Respiratory Journal. 17(6), 1059-61
• KNOX, A.J., PANG, L.H. and ZHU, Y.M., 2001. Targetting remodelling in respiratory inflammation Current Opinion in Investigational Drugs. 1, 428-434
• PANG, L, 2001. COX-2 expression in asthmatic airways: the story so far. Thorax. 56(5), 335-6
• PANG, L and KNOX, A J, 2000. Synergistic inhibition by beta(2)-agonists and corticosteroids on tumor necrosis factor-alpha-induced interleukin-8 release from cultured human airway smooth-muscle cells. American Journal of Respiratory Cell and Molecular Biology. 23(1), 79-85
• PANG, L, HOLLAND, E and KNOX, A J, 1998. Role of cyclo-oxygenase-2 induction in interleukin-1beta induced attenuation of cultured human airway smooth muscle cell cyclic AMP generation in response to isoprenaline. British Journal of Pharmacology. 125(6), 1320-8
• PANG, L and KNOX, A J, 1998. Bradykinin stimulates IL-8 production in cultured human airway smooth muscle cells: role of cyclooxygenase products. The Journal of Immunology. 161(5), 2509-15
• PANG, L and KNOX, A J, 1997. Effect of interleukin-1 beta, tumour necrosis factor-alpha and interferon-gamma on the induction of cyclo-oxygenase-2 in cultured human airway smooth muscle cells. British Journal of Pharmacology. 121(3), 579-87