Associate Professor of Biochemistry and Pathology, Faculty of Medicine & Health Sciences
Kevin Gough graduated with a BSc (Hons) in Biochemistry from the University of Wales, Aberystwyth (1992). He then obtained a PhD in Biochemistry from Aberystwyth, graduating in 1996. His post-doctoral employment includes three years as a research associate at The University of Leicester before becoming a Research Scientist with ADAS. Within 8 years at ADAS, his roles progressed through Senior Research Scientist to Principal Research Scientist and Leader of the ADAS Biotechnology Group. Kevin joined the School of Veterinary Medicine and Science as a Lecturer in Molecular Biochemistry in 2007 and became Associate Professor of Biochemistry and Pathology in 2012.
Kevin Gough is Associate Professor of Biochemistry and Pathology within the SVMS.
He has acted as an independent scientific expert on appraisal panels for the Defra TSE Review 2007, for the Food Standards Agency TSE Research Programme Review 2007 and for the MRC Prion Research Reviews 2009 and 2014. He is also an Editorial Board Member for the journal Scientific Reports.
He has extensive experience of research in Molecular Biology and Biochemistry. Techniques include: prion diagnostics, recombinant antibody production, protein purification, monoclonal antibody production, diagnostic PCR and Q-PCR, enzyme kinetics and immunoassay development, next generation phage display, next generation sequencing, biomarker discovery. He also has extensive experience of project and staff management, contract management, bid preparation, results dissemination, business development and commercialisation.
My research is mainly focused on studying infections of livestock with a particular emphasis on developing novel diagnostics or therapeutics. The pathogens under investigation include prions (BSE and… read more
MADDISON, B.C., REES, H.C., BAKER, C.A., TAEMA, M., BELLWORTHY, S.J., THORNE, L., TERRY, L.A. GOUGH, K.C. and GOUGH, K.C., 2010. Prions are secreted into the oral cavity in sheep with preclinical scrapie Journal of Infectious Diseases. 201(11), 1672-1676 MADDISON, B.C., BAKER, C.A., REES, H.C., TERRY, L.A., THORNE, L., BELLWORTHY, S.J., WHITELAM G.C. and AND GOUGH, K.C., 2009. Prions are secreted in milk from clinically normal scrapie-exposed sheep Journal of Virology. 83(16), 58578 GOUGH, K.C., BAKER, C.A., REES, H.C., TERRY, L.A., SPIROPOULOS, J., THORNE, L. and MADDISON, B.C., 2012. The oral secretion of scrapie prions occurs in pre-clinical sheep with a range of PRNP genotypes Journal of Virology. 86(1), 566-571
MADDISON, B.C., BAKER, C.A., TERRY, L.A., BELLWORTHY, S.J., THORNE L, and REES, L., 2010. Environmental sources of scrapie prions Journal of Virology. 84(21), 11560-1562
Research Group Members:
Claire Baker (ADAS UK): Research areas: Development of a pen-side test for liver fluke infections of ruminants. Understanding the environmental dissemination of prions and routes of disease transmission; Assessing on-farm prion decontamination methods.
Dr Jon Owen (ADAS UK): Research areas: Next generation phage display applied to the development of a pen-side test for Schmallenberg virus infections of ruminants and the development of novel vaccines to liver fluke infection.
Keith Bishop (ADAS UK): Research areas: The application of environmental DNA detection to monitor for Great Crested Newt.
Anastasios Spiliotopoulos: Research areas: Next generation phage display; Isolation of novel ligands against human ubiquitin specific proteases and in immunoproteomics for the identification of biomarkers.
Ibrahim Naqid: Research areas: Next generation phage display; Mapping antibody-mediated immune responses to Salmonella enterica infections
Matthew O'Connor: Research areas: In vitro prion replication assays; The replacement of animal bioassays that measure prion infectivity.
Robert Workman: Research areas: The discovery of therapeutic and diagnostic ligands for ruminant prion diseases.
Ceri Staley: Research areas: Next generation phage display; Development of a pen-side test for Schmallenberg virus infections of ruminants.
Katarzyna Zolnierczyk: Research area: Development of a vaccine for liver fluke infections of ruminants.
Rose Reader: Research area: Next generation phage display; The isolation of immunoreagents for application within the animal feed industry.
Amanda Robinson: Research areas: The application of environmental DNA detection to monitor for Great Crested Newt.
Dr Ben Maddison (ADAS UK)
Dr Helen Rees (ADAS UK)
Dr Janet Daly (SVMS, University of Nottingham)
Dr Rachael Tarlinton (SVMS, University of Nottingham)
Dr Robin Flynn (SVMS, University of Nottingham)
Dr Richard Emes (SVMS, University of `Nottingham)
Dr Ingrid Dreveny (Pharmacy, University of Nottingham)
Professor Roberto La Ragione (University of Surrey)
My research is mainly focused on studying infections of livestock with a particular emphasis on developing novel diagnostics or therapeutics. The pathogens under investigation include prions (BSE and scrapie), Schmallenberg virus, Fasciola hepatica and Salmonella enterica. Research is also carried out into the application of molecular diagnostic methods to monitor endangered wildlife species.
Prion Diseases. Prion diseases or transmissible spongiform encephalopathies (TSEs) are fatal neurological disorders that are marked by long incubation periods. TSEs have been described in a number of mammalian species and include scrapie in sheep and goats, bovine spongiform encephalopathy (BSE) in cattle, chronic wasting disease in deer and elk and Creutzfeldt-Jakob disease (CJD) in humans. According to the protein only hypothesis, the infectious agent of these diseases is formed by the misfolding of a benign cellular prion protein (PrPC) into a protease-resistant conformation (PrPSc). These diseases are irreversible and invariably fatal; there is compelling evidence that the BSE agent has crossed the species barrier and is the causal agent of vCJD in humans; and the causal agent appears to be novel, an infectious misfolded version of a host protein which encodes transmissible strain characteristics in the absence of any detectable nucleic acid. Our research includes the elucidation of the routes of scrapie transmission and the environmental fate of the TSE agent, the strain typing of ruminant TSEs to study emerging strains, and the pre-mortem diagnosis of TSE diseases.
Schmallenberg virus (SBV) is a newly emerged virus affecting sheep, cattle and goats which is currently causing a large- scale outbreak of foetal deformities across Europe, and has become endemic within the UK ruminant population. Our research is leading to the development of pen-side tests for the detection of exposure to SBV and also to the development of assays to differentiate vaccinated from infected animals (so-called DIVA tests).
Fasciola hepatica is a major parasite of cattle and sheep which causes livestock mortality and reductions in productivity. Our research aims to produce a lateral flow device for simple, rapid diagnosis of fasciolosis at the pen-side and to provide evidence of the life-stage of F. hepatica. We are also developing novel multi-component vaccines against F. hepatica.
Salmonella infections place significant economic burdens on livestock industries and zoonotic serovars represent some of the most common foodborne pathogens affecting human health. There is an urgent requirement to further understand the pathobiology of these pathogens, as well as develop efficacious vaccines. Our research aims to map host antibody responses to Salmonella infections to identify Salmonella immunogens for diagnostic assay development and subunit vaccine design.
Ligand-display technology: Our design of novel diagnostics is centered around ligand display systems and particularly the display of recombinant antibody fragments and peptides on filamentous bacteriophage. Peptide phage-display libraries have proved useful for mapping epitopes of monoclonal antibodies, and the isolation of ligands that bind specifically to a range of target molecules. This technology also allows the isolation of peptides capable of disrupting specific protein-protein interactions. In the form of antibody phage-display libraries this technology is a powerful in vitro technique that allows the selection of antibodies with defined properties from vast libraries of binders. This allows the isolation of antibodies with exquisite selectivity between closely related molecules, and by removing the need for immunization it allows the production of antibodies against toxic or non-antigenic molecules. To date, we have applied phage-display methods to isolate ligands against a wide range of molecular targets including host cell surfaces, pathogen surface molecules, proteins, peptides and haptens. In recent years we have coupled the vast diversity of phage display libraries with the screening power of next generation sequencing, a process called next generation phage display (NGPD). This novel technique allows the highly efficient selection of a wide range of specific ligands. NGPD is being applied in our research to improve diagnostics and develop novel therapeutics against veterinary pathogens and zoonoses.
eDNA detection for surveillance of wildlife species: We are applying real time PCR and Next Generation Sequencing methods to detect environmental DNA (eDNA) of wildlife species in aquatic environments. The research is primarily aimed at the detection of the Great Crested Newt, a protected species.
SPILIOTOPOULOS, A, OWEN, JP, MADDISON, BC, DREVENY, I, REES, HC and GOUGH, KC, 2015. Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets J Immunol Meth. (In Press.)
DALY, JM, KING, B, TARLINTON, R, GOUGH, KC, MADDISON, BC and BLOWEY, R, 2015. Comparison of Schmallenberg virus antibody levels detected in milk and serum from individual cows BMC Vet Res. (In Press.)
BC MADDISON, J SPIROPOLOUS, CM VICKERY, R LOCKEY, JP OWEN, K BISHOP, CA BAKER and KC GOUGH, 2015. Incubation of ovine scrapie with environmental matrix results in biological and biochemical changes of PrPSc over time Vet Res. (In Press.)
KC GOUGH, CA BAKER, HA SIMMONS, SA HAWKINS and BC MADDISON, 2015. Circulation of prions within dust on a scrapie affected farm Vet Res. (In Press.)
SMITH, C.M, FRY, S.C., GOUGH, K.C., PATEL, A.J.F., GLENN, S., HAWES, W.H., GOLDRICH, M., ROBERTS, I.S., WHITELAM, G.C. and ANDREW. P.W., 2014. Recombinant Plants Provide a New Approach to the Production of Bacterial Polysaccharide for Vaccines PLoSONE. 9(2), e88144 REES, H.C., GOUGH, K.C., MIDDLEDITCH, D.J., PATMORE, J.R.M. and MADDISON, B.C., 2014. The Detection of Aquatic Animal Species using Environmental DNA - a review of eDNA as a survey tool in Ecology Journal of Applied Ecology. REES, H.C., BISHOP, K., MIDDLEDITCH, D.J., PATMORE, J.R.J., MADDISON, B.C. and GOUGH, K.C., 2014. The application of eDNA for monitoring of the Great Crested Newt in the UK Ecology and Evolution. NAQID, IA, OWEN, JP, MADDISON, BC, GARDNER, DS, FOSTER, N, TCHÓRZEWSKA, MA, LA RAGIONE, RM and GOUGH, KC, 2014. Prebiotic and probiotic agents enhance antibody-based immune responses to Salmonella Typhimurium infection in pigs Animal Feed Science and Technology. GOUGH, K.C., BAKER, C.A., REES, H.C., TERRY, L.A., SPIROPOULOS, J., THORNE, L. and MADDISON, B.C., 2012. The oral secretion of scrapie prions occurs in pre-clinical sheep with a range of PRNP genotypes Journal of Virology. 86(1), 566-571
MADDISON, B.C. AND GOUGH K.C., 2012. Scrapie dissemination and environmental persistence Goat Vet. Soc. J.. 28, 25-32
MADDISON, B.C., OWEN, J.P., TAEMA, M.M., SHAW, G. and GOUGH, K.C., 2012. Temperature influences the interaction of ruminant PrPTSE with soil Prion. 6(3), 302–308
THORNE, L, HOLDER, T, RAMSAY, A, EDWARD, J, TAEMA, M, MADDISON, B C, GOUGH, K C and TERRY, L A, 2012. In vitro amplification of ovine prions from scrapie-infected sheep from Great Britain reveals distinct patterns of propagation BMC Vet Res. In press, (In Press.)
TERRY L.A, HOWELLS, L, BISHOP K, BAKER C.A, EVEREST S, THORNE L and MADDISON B.C. AND GOUGH K.C., 2011. Detection of prions in the faeces of sheep naturally infected with scrapie Vet. Res.. 42, 65 GOUGH, K.C and JARVIS, S. AND MADDISON B.C., 2011. Development of competitive immunoassays to hydroxyl containing fungicide metabolites Env. Sci. Health, Part B.. 46, 581-589 TARLINTON, RE, YON, L, KLISCH, K, TOTEMEYER, S and GOUGH, KC, 2011. Confidence As A Barrier To The Use Of Problem-Based Learning In Veterinary Undergraduate Students Journal Of Veterinary Medical Education. 38(3), 305-310 TAEMA MM, MADDISON BC, THORNE L, BISHOP K, OWEN J, HUNTER N, BAKER CA, TERRY LA and GOUGH KC, 2011. Differentiating Ovine Bse From Ch1641 Scrapie By Serial Protein Misfolding Cyclic Amplification. Molecular Biotechnology. 51(3), 233-9 FEATHER, L, GOUGH, K and FLYNN, R.J. AND ELSHEIKHA, H., 2010. A retrospective investigation into risk factors of sarcoptic mange in dogs. Parasitology Research. 107, 279-283
MADDISON B.M, OWEN, J.P, BISHOP, K, SHAW, G and REES H.C. AND GOUGH K.C., 2010. The interaction of ruminant PrPSc with soils is influenced by prion source and soil-type Env. Sci. Technol.. 44, 8503-8508.
MADDISON, B.C., BAKER, C.A., TERRY, L.A., BELLWORTHY, S.J., THORNE L, and REES, L., 2010. Environmental sources of scrapie prions Journal of Virology. 84(21), 11560-1562 MADDISON, B.C., REES, H.C., BAKER, C.A., TAEMA, M., BELLWORTHY, S.J., THORNE, L., TERRY, L.A. GOUGH, K.C. and GOUGH, K.C., 2010. Prions are secreted into the oral cavity in sheep with preclinical scrapie Journal of Infectious Diseases. 201(11), 1672-1676 MADDISON, B.C., BAKER, C.A., REES, H.C., TERRY, L.A., THORNE, L., BELLWORTHY, S.J., WHITELAM G.C. and AND GOUGH, K.C., 2009. Prions are secreted in milk from clinically normal scrapie-exposed sheep Journal of Virology. 83(16), 58578 REES, H.C, MADDISON, B.C, OWEN, J.P and WHITELAM, G.C. AND GOUGH, K.C., 2009. Concentration of disease-associated prion protein with silicon dioxide. Molecular Biotechnology. 41, 254-262 OWEN, J.P., REES, H.C., MADDISON, B.C., TERRY, L.A., THORNE, L., JACKMAN, R., WHITELAM, G.C. and GOUGH, K.C., 2007. Molecular profiling of ovine prion diseases by using thermolysin-resistant PrPSc and endogenous C2 PrP fragments Journal of Virology. 81(19), 10532-10539 MADDISON, B.C., WHITELAM, G.C. and GOUGH, K.C., 2007. Cellular prion protein in ovine milk. Biochemical and Biophysical Research Communications. 353(1), 195-199 OWEN, JONATHAN P, MADDISON, BEN C, WHITELAM, GARRY C and GOUGH, KEVIN C, 2007. Use of thermolysin in the diagnosis of prion diseases. Molecular Biotechnology. 35(2), 161-70 LA RAGIONE, R.M., PATEL, S., MADDISON, B., WOODWARD, M.J., BEST, A., WHITELAM, G.C. and GOUGH, K.C., 2006. Recombinant anti-EspA antibodies block Escherichia coli O157:H7-induced attaching and effacing lesions in vitro. Microbes and Infection. 8(2), 426-433 MADDISON, B.C., PATEL,S., JAMES, R.F., CONLON, H.E., OIDTMANN, B., BAIER, M., WHITELAM, G.C and GOUGH, K.C., 2005. Generation and characterisation of monoclonal antibodies to Rainbow trout (Oncorhynchus mykiss) prion protein. Journal of Immunological Methods. 306, 202-210. KÜHNE, S.A., HAWES, W.S., LA RAGIONE, R.M., WOODWARD, M.J., WHITELAM, G.C. and GOUGH, K.C., 2004. Isolation of recombinant antibodies against EspA and intimin of Escherichia coli O157:H7. Journal of Clinical Microbiology. 42(7), 2966-2976
GOUGH, K.C. and WHITELAM, G.C., 2003. Production of Antibodies using Transgenic Plants. In: SINGH, R.P. and JAIWAL, P.K., eds., Plant genetic engineering: Applications and limitations 1. Sci Tech Publishing LLC, U.S.A.. 261-277
GOUGH, K.C., LI, Y. and WHITELAM, G.C., 2002. Phage-Display Libraries. In: GILMARTIN, P. and BOWLER, C., eds., Molecular Aspects of Plant Biology: Practical Approach Series IRL Press Ltd., Eynsham, Oxford. 221-236
BISHOP, K, GOUGH, K, MAHONEY, S, SMITH, A and ROGERS, L, 1999. Synechococcus mutants resistant to an enamine mechanism inhibitor of glutamate-1-semialdehyde aminotransferase. FEBS Letters. 450(1-2), 57-60 GOUGH, K C, COCKBURN, W and WHITELAM, G C, 1999. Selection of phage-display peptides that bind to cucumber mosaic virus coat protein. Journal of Virological Methods. 79(2), 169-80
GOUGH, K C, LI, Y, VAUGHAN, T J, WILLIAMS, A J, COCKBURN, W and WHITELAM, G C, 1999. Selection of phage antibodies to surface epitopes of Phytophthora infestans. Journal of Immunological Methods. 228(1-2), 97-108