Teaching Western Blots with T antigen and p53
Students perform Western blots with polyacrylamide gel electrophoresis of detergent extracts of SV40-transformed cell lines grown in monolayer culture. The gels are blotted onto nitrocellulose and treated with a monoclonal antibody against SV40 T antigen or against the tumor suppressor p53. Blots are then treated with a secondary antibody (anti-mouse IgG conjugated to horseradish peroxidase), and exposed to revealing chemicals and x-ray film. I give one application: induction of p53 by ultraviol
Using the Worm, Caenorhabditis elegans, in Undergraduate Genetics and Developmental Biology Laborato
C. elegans is easy to use, transparent, reproduces quickly, and has a cloned genome. All of these factors make it an ideal system for use in introductory, genetics or developmental biology labs. This lab familiarizes students with the life cycle, development, terminology, and common mutant phenotypes of C. elegans. It also introduces the use of GFP transgenic worms to visualize specific cell types in the worm. A detailed sample experiment is included that takes students through using genetic cro
In this hands-on activity, students build their own two-cell battery. They also determine which electrolyte solution is best suited for making a battery.
The Holloway Series in Poetry: Joshua Clover
Lively, stylish and "totally great" poetry from a poet of "fierce power." With graduate poet Colin Dingler. Clover's work has been praised by theorist Judith Butler for the way that it "brilliantly queries our historical moment in and as form" and it is with an intellectual clarity and linguistic sharpness that he undertakes these poetic investigations. Stylish, textured, intricate, irreverent-- Clover's work is studded with intensity, "social passion" and delight. This is what Wallace Stevens
Learn with Pictures and Video S4 #20 - Don’t Shop in Japanese Without These Words
You’re at the Mall of Japan, and you can’t get over how huge it is! It’s so big that you get a little overwhelmed and forget the Japanese words for what you came to buy. You look in a few stores and speak Japanese with a few people, and quickly you regain the confidence you [...]
Female desert bighorn sheep in the Sonora desert
Desert bighorn sheep use a lot of energy to regulate their internal temperature in the desert. During the summer the sheep eat plants and drink water every few days. In the winter, desert plants contain enough water for sheep survival.
The intestine and anus of the Daphnia
The intestine and anus are part of the digestive system. In animals, the complete digestive system is made up of the mouth, pharynx, esophagus, stomach, intestines, pancreas, and the anus. The digestive system functions to process food and eliminate wastes.
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Got Energy? Spinning a Food Web
Students learn about energy flow in food webs, including the roles of the sun, producers, consumers and decomposers in the energy cycle. They model a food web and create diagrams of food webs using their own drawings and/or images from nature or wildlife magazines. Students investigate the links between the sun, plants and animals, building their understanding of the web of nutrient dependency and energy transfer.
Prepare sample - Perform Instrumental Tests/Procedures
An introduction to the use of specific testing equipment in scientific and medical testing laboratories. Covers sample preparation procedures used in food and beverage testing.
Infosys leaders on leadership
When Infosys Technologies Ltd. founder and CEO S. Gopalakrishnan addressed INSEAD as part of the Global Leadership Series in March, the 55-year-old corporate titan said he believes in leading by example. Now, the world is about to see if he practices what he preaches.
MOLA: Seasonal Snow Variations on Mars: Slow Zoom out from the Martian North Pole: True Color
This is a true color visualization of the topography near the Martian north pole as measured with the MOLA instrument. This particular animation shows a slow zoom out from the surface of the pole and is a frame-accurate match to the end of animation id #2297.
Latitude and Longitude
This video shows the Latitude and Longitude lines on a globe with Washington DC. Latitude lines run east to west and its zero degree is the equator. Longitude lines run north to south and has a prime meridian in Greenwich, England. To make latitude and longitude lines more accurate you can split the degree into Minutes and Seconds. The exact measurements for Washington DC is 38 degree 53'22''
North and 77 degree 2'41'' West. This cartoon video explains how the lines were used and why they
Louer ou acheter?
Op het einde van deze les kun je de voor- en nadelen van het kopen van een eigendom opsommen.
Learn Hindi Daily Show – I like Hindi. What? Last of the Clever Crow story.
“I like Hindi.” is something that we all should be able to say. Once we know this phrase, we will be able to say that we like anything. Let’s take...
Great Zoom out of Tucson, AZ: The University of Arizona Football Stadium
Using data from different spacecraft and some powerful computer technology, visualizers at the Goddard Space Flight Center present you with a collection of American cities in a way you have never seen them before. Starting with our camera high above the Earth, we rush in towards the surface at what would be an impossible speed for any known vehicle. Passing though layers of atmosphere, the colors of our destinations shimmer with their own unique characteristics, and suddenly we find ourselves fl
Neuroscience in the Courtroom
Walter Sinnott-Armstrong, Leverhulme Visiting Professor. Please note there is some interference on this recording.
Where the Shape of the Egg Comes From?
The shape of the egg is postulated to be the consequence of opposing forces relative to the location of perspective of points that define the spaces of its' contents. For example, net velocities of motion of light emitted in two planes plus the velocity of motion of its' source would be expected to be decreased in the case when the direction of the light beam is opposed to the direction of motion to cause the blunt edge and increased when it aligns with the direction of motion of its' source t