Virtual laboratories in Molecular and Cell Biology - SDS-PAGE
A protein analysis laboratory using SDS-PAGE, western-blotting and endoprotease digestion. The programme includes a section on the theory of the techniques, a video demonstration in a real laboratory, and a series of questions which guide the students through the structural analysis of model proteins (3 are included). Students go to the virtual laboratory and devise their own experiments in order to determine the molecular weight, subunit composition etc of the proteins. Results (gels and blots)
DNA: properties and applications in molecular biology
These slides provides an overview of some of the properties of nucleic acids (DNA and RNA) and its applications in molecular biology. It gives an overview of the topology of DNA and how it can be exploited in PCR, restriction mapping and other molecular techniques. The resource is used as a 50min lecture to first year undergradutes in the biosciences. It was created by Dr. Momna Hejmadi and the source file can be adapted for educational and non-commercial purposes only as licensed under the C
7.16 Experimental Molecular Biology: Biotechnology II (MIT)
The course applies molecular biology and reverse genetics approaches to the study of apoptosis, or programmed cell death (PCD), in Drosophila cells. RNA interference (RNAi), or double stranded RNA-mediated gene silencing, will be used to inhibit expression of candidate apoptosis-related genes in cultured Drosophila cells. Teams of 2 or 3 students will design and carry out experiments to address questions about the genes involved in the regulation and execution of PCD in this system. Some project
10.675J Computational Quantum Mechanics of Molecular and Extended Systems (MIT)
The theoretical frameworks of Hartree-Fock theory and density functional theory are presented in this course as approximate methods to solve the many-electron problem. A variety of ways to incorporate electron correlation are discussed. The application of these techniques to calculate the reactivity and spectroscopic properties of chemical systems, in addition to the thermodynamics and kinetics of chemical processes, is emphasized. This course also focuses on cutting edge methods to sample compl
Understanding the Molecular Networks of Hydrogen Production
Eric Schadt, Ph.D., Pacific Biosciences Environmental concerns over the use of fossil fuels and their role in climate change have sparked research on the development of alternative fuels. Hydrogen is a clean burning alternative fuel that can be produced in large amounts by some bacteria. We have embarked on a systems level approach to dissect metabolic and regulatory networks necessary for nitrogenase-catalyzed hydrogen production by the bacterium Rhodopseudomonas. Rhodopseudomonas is an ideal
Molecular reaction dynamics
This package, written in 1998, includes interactive questions and demonstrations on the dynamics of chemical reactions. The aim is to show the effect of the potential energy surface, on reaction rates. It is intended for third or fourth year undergraduates in Chemistry. To download, click on View Download and follow the instructions. To uninstall, use the standard Windows option of “Add or Remove Programs”.
Introduction to Molecular Spectroscopy
This guide provides an introduction to the different types of molecular spectroscopic analysis, describing the basic principles of each technique and practical considerations including sample preparation. It is illustrated with simple diagrams, photographs of equipment and information to aid interpretation of spectra.
Molecular Biology and Genetics in Modern Medicine Geneticists have discovered that "junk" genes that lie dead in our DNA can come back to life and cause muscular dystrophy. Scientists such as MIT's David Housman
Geneticists have discovered that "junk" genes that lie dead in our DNA can come back to life and cause muscular dystrophy. Scientists such as MIT's David Housman
12.458 Molecular Biogeochemistry (MIT)
This course evaluates and discusses the formation and diagnostic structural properties of organic compounds with particular emphasis on those molecules which form chemical fossils. The course is structured around the biosynthetic and phylogenetic origins of recalcitrant hydrocarbons.
HST.730 Molecular Biology for the Auditory System (MIT)
An introductory course in the molecular biology of the auditory system. First half focuses on human genetics and molecular biology, covering fundamentals of pedigree analysis, linkage analysis, molecular cloning, and gene analysis as well as ethical/legal issues, all in the context of an auditory disorder. Second half emphasizes molecular approaches to function and dysfunction of the cochlea, and is based on readings and discussion of research literature.
9.530 Cellular and Molecular Computation (MIT)
Life as an emergent property of networks of chemical reactions involving proteins and nucleic acids. Mathematical theories of metabolism, gene regulation, signal transduction, chemotaxis, excitability, motility, mitosis, development, and immunity. Applications to directed molecular evolution, DNA computing, and metabolic and genetic engineering.
18.417 Introduction to Computational Molecular Biology (MIT)
This course introduces the basic computational methods used to understand the cell on a molecular level. It covers subjects such as the sequence alignment algorithms: dynamic programming, hashing, suffix trees, and Gibbs sampling. Furthermore, it focuses on computational approaches to: genetic and physical mapping; genome sequencing, assembly, and annotation; RNA expression and secondary structure; protein structure and folding; and molecular interactions and dynamics.
HST.176 Cellular and Molecular Immunology (MIT)
This course covers cells and tissues of the immune system, lymphocyte development, the structure and function of antigen receptors, the cell biology of antigen processing and presentation, including molecular structure and assembly of MHC molecules, the biology of cytokines, leukocyte-endothelial interactions, and the pathogenesis of immunologically mediated diseases. The course is structured as a series of lectures and tutorials in which clinical cases are discussed with faculty tutors. Lecture
7.28 Molecular Biology (MIT)
This course covers a detailed analysis of the biochemical mechanisms that control the maintenance, expression, and evolution of prokaryotic and eukaryotic genomes. The topics covered in lectures and readings of relevant literature include gene regulation, DNA replication, genetic recombination, and mRNA translation. In particular, the logic of experimental design and data analysis is emphasized.
20.450 Molecular and Cellular Pathophysiology (BE.450) (MIT)
This course focuses on the fundamentals of tissue and organ response to injury from a molecular and cellular perspective. There is a special emphasis on disease states that bridge infection, inflammation, immunity, and cancer. The systems approach to pathophysiology includes lectures, critical evaluation of recent scientific papers, and student projects and presentations. This term, we focus on hepatocellular carcinoma (HCC), chronic-active hepatitis, and hepatitis virus infections. In addition
20.442 Molecular Structure of Biological Materials (BE.442) (MIT)
This course, intended for both graduate and upper level undergraduate students, will focus on understanding of the basic molecular structural principles of biological materials. It will address the molecular structures of various materials of biological origin, such as several types of collagen, silk, spider silk, wool, hair, bones, shells, protein adhesives, GFP, and self-assembling peptides. It will also address molecular design of new biological materials applying the molecular structural pri
20.410J Molecular, Cellular and Tissue Biomechanics (BE.410J) (MIT)
This course develops and applies scaling laws and the methods of continuum mechanics to biomechanical phenomena over a range of length scales. Topics include: structure of tissues and the molecular basis for macroscopic properties; chemical and electrical effects on mechanical behavior; cell mechanics, motility and adhesion; biomembranes; biomolecular mechanics and molecular motors. Experimental methods for probing structures at the tissue, cellular, and molecular levels will also be investigate
10.520 Molecular Aspects of Chemical Engineering (MIT)
This class covers molecular-level engineering and analysis of chemical processes. The use of chemical bonding, reactivity, and other key concepts in the design and tailoring of organic systems are discussed in this class. Specific class topics include application and development of structure-property relationships, and descriptions of the chemical forces and structural factors that govern supramolecular and interfacial phenomena for molecular and polymeric systems.
20.462J Molecular Principles of Biomaterials (MIT)
This course covers the analysis and design at a molecular scale of materials used in contact with biological systems, including biotechnology and biomedical engineering. Topics include molecular interactions between bio- and synthetic molecules and surfaces; design, synthesis, and processing approaches for materials that control cell functions; and application of state-of-the-art materials science to problems in tissue engineering, drug delivery, vaccines, and cell-guiding surfaces.
12.458 Molecular Biogeochemistry (MIT)
This course covers all aspects of molecular biosignatures from their pathways of lipid biosynthesis, the distribution patterns of lipid biosynthetic pathways with regard to phylogeny and physiology, isotopic contents, occurrence in modern organisms and environments, diagenetic pathways, analytical techniques and the occurrence of molecular fossils through the geological record.