Cell Fractionation in Tetrahymena
To illustrate cell fractionation, nuclei are isolated from the ciliated protozoan, Tetrahymena thermophila. A table top clinical centrifuge is used for the fractionation steps and the procedure is monitored microscopically using a differential stain. To determine the efficiency of the procedure, cell and nuclear counts are determined with a hemacytometer. To quantify DNA, the Diphenylamine Reaction is carried out and the amount of DNA per nucleus is calculated.
Benefits of Standardized Diabetes and Hypertension Screening Forms at Community Screening Events
The objectives of this project were to (a) assess hypertension and diabetes screening data collection practices and guidelines and (b) develop and test standardized screening forms for use at minority community- and faith-based screening events. Project Phase I involved resource assessment and the development of a set of screening forms and guidelines containing a core data set for both hypertension and diabetes. These were then tested during Phase II at predetermined communitybased screening ev
Cyanobacteria Health Page
This Centers for Disease Control and Prevention (CDC) Health Studies page focuses on cyanobacteria, single-celled organisms thought to be the origin of plants. Cyanobacteria live in fresh, brackish, or marine water and are of concern to the CDC and Environmental Protection Agency (EPA) because some can form harmful blooms that deplete the oxygen and block sunlight that other organisms need to live. They can also produce powerful toxins that affect the brain and liver of animals and humans. This
The Greenomes site is part of a laboratory- and Internet-based curriculum to bring college students up to the minute with modern plant research. Plant molecular genetic and genomic research still lags behind medically-oriented research on microbes and higher animals. As a result, there are relatively few lab experiences that expose college-level students to the growing insights into plants offered by genomic biology.
Detection of Genetically Modified Foods
Genetically modified foods are often in the news and widely grown in the United States. Three US government agencies (USDA, FDA, and EPA) work to regulate the introduction and production of genetically modified foods. These crops can provide agricultural, ecological and nutritional benefits, but there are also potential risks to the environment and consumers. As consumers and public interest groups around the world have become aware of these risks, there has been a call for more explicit product
Chemiosmotic Principles of Solute Transport in Barley Roots
This exercise will demonstrate to students the fluxes of certain inorganic ions in the roots of barley plants. It will familiarize them with the chemiosmotic principles that are involved in ATP synthesis and give them an understanding of solute transport.
Bacterial Quorum Sensing and Bioluminescence
The bacterium Vibrio fischeri produces light when it is growing symbiotically in the light organ of certain fish and squid species. The bacteria sense they are in a light organ because they are present at a high bacterial cell density, which they detect through "quorum sensing." We use several bacterial strains, each of which carry only part of the genes responsible for the quorum sensing and bioluminescence process. We use chemical and genetic complementation to determine which genes are lackin
Vegetation Images Show Drought in Western US (WMS)
Satellite data can gauge the health of plants, which is a good indicator of drought. The Normalized Difference Vegetation Index (NDVI) measures how dense and green plant leaves are. NDVI images are useful as a measure of drought when compared to "normal" plant health. Scientists calculate average NDVI values for an area to find out what is normal at a particular time of year. This animation uses satellite imagery to show changes in vegetation between 1999 and 2003. In 2002, drought had settled a
The Rise of Multicultural Managers
Many multinational companies have hidden, unrecognised multicultural gems within their ranks. To find these and get the most from their unique skills means taking the time and trouble to carefully develop and deploy multicultural managers in critical positions.
Advanced semiconductor devices are a new source of energy for the 21st century, delivering electricity directly from sunlight. Suitable semiconductor materials, device physics, and fabrication technologies for solar cells are presented in this course. The guidelines for design of a complete solar cell system for household application are explained. Cost aspects, market development, and the application areas of solar cells are presented.
Stem Cells: 5 Year Progress Report
Embryonic stem cells can transform themselves into every cell in your body. Does researching these stem cells violate human sanctity, or is it medicine's brightest frontier? What are the prospects for adult stem cells?
7.3 Disposal requirements
Health, safety and risk assessment are of paramount importance both in the laboratory and the field. This unit will help make you more aware of the hazards and risks involved in laboratory and field-based research work, as well as giving you an overview of the legal requirements attached to this work. The unit discusses issues involved in the handling chemical and biological agents, basic safety procedures and common field-work hazards.
Medicines by Design
Medicines By Design aims to explain how scientists unravel the many different ways medicines work in the body and how this information guides the hunt for drugs of the future. Pharmacology is a broad discipline encompassing every aspect of the study of drugs, including their discovery and development and the testing of their action in the body. Much of the most promising pharmacological research going on at universities across the country is sponsored by the National Institute of General Medical
Monitor and review effectiveness of treatment of risk
In this task we'll monitor and review the effectiveness of the risk controls we have implemented. It includes a learning pack which describes the need to constantly review any risk management program, to ensure that the chosen solution is working and that there are no new risks arising. You will also find out about the importance of using the results of current risk management techniques to constantly improve the overall risk management culture of the organisation.
Latin America and the Caribbean: Peace Corps
provides lessons around stories, letters, poems, and folk tales from experiences of Peace Corps volunteers. Topics include the geography and cultures of the Dominican Republic, hurricanes, hero worship, conducting interviews, Paraguay, the risks of a one-crop economy (coffee), how best to use one's time in different cultures, why service to others matters, and the common good.
Essential Science for Teachers: Physical Science: Session 2. The Particle Nature of Matter: Solids,
In-depth interviews with children that uncover their ideas about the topic at hand.,The segment provides examples of questions used to probe the student's ideas about particles, particularly, that substances such as air are made up of invisible tiny particles called atoms that are far too small to be seen through a regular microscope. When asked if he could draw air, the student responds that air cannot be drawn because it is just a bunch of invisible particles called atoms, but when probed furt
2.4 Critical magnetic field
The fascinating phenomenon of superconductivity and its potential applications have attracted the attention of scientists, engineers and businessmen. Intense research has taken place to discover new superconductors, to understand the physics that underlies the properties of superconductors, and to develop new applications for these materials. In this unit you will read about the history of superconductors, taking a brief look at their properties. You will also learn about modelling the propertie
SAGP Guide to Clinical Medical Practice
Manual originating from personal notes made over many years of semi-rural private and hospital practice in South Africa. A useful resource based on many years of semi-rural private and hospital practice in South Africa involving major trauma, surgery and obstetrics, in addition to the common problems of general practice. I have always felt it very useful to be able to confirm facts and figures at the bedside if needed and this has been the motivation that has taken me over 40 years. This meant
The News: HeartMate II
In this commercial, we open on a doctor reading a thank-you letter from a patient. Dr. Jennifer Peura tells us how in medical school, she dreamed of finding all the answers for her patients. She explains how heart patients for years were tethered to a giant device, until MUSC acquired a device the size of a cell phone to replace their failing hearts. She is summoned to a patient room, where we see that her heart patient is now a proud uncle, celebrating the birth of his niece.
Model of the monoclinic ZrO2 unit cell
Rotating model of the monoclinic ZrO2 unit cell.