Purification of Maltose-Binding Protein from E. coli Periplasm
By correlating the protein concentration of various fractions from E. coli with the appearance of samples on a gel, this exercise introduces beginning students to the quantitative "bookkeeping" involved in protein purification. A periplasmic fraction is isolated by osmotic shock, and affinity chromatography with amylose resin is used to purify maltose-binding protein. Visualization of fractions by SDS-polyacrylamide gel electrophoresis allows students to evaluate the qualitative success of the p
Beginner - Answering the door There are etiquettes that should be followed when knocking the door. Many of us are oblivious to most, particularly to the one on how to announce oneself. Today you will learn what you should say when knocking the door.
There are etiquettes that should be followed when knocking the door. Many of us are oblivious to most, particularly to the one on how to announce oneself. Today you will learn what you should say when knocking the door.
02.08.2011 – Langsam gesprochene Nachrichten
Trainieren Sie Ihr Hörverstehen mit authentischen Materialien. Nutzen Sie die Nachrichten der Deutschen Welle von Dienstag – als Text und als verständlich gesprochene Audio-Datei.Nach wochenlangem Nervenkrieg im US-Schuldenstreit hat der Kompromiss zwischen Demokraten und Republikanern die erste und wahrscheinlich entscheidende parlamentarische Hürde genommen. Nur einen Tag, bevor die weltgrößte Volkswirtschaft zahlungsunfähig geworden wäre, billigte das von Republi
Israel unveils missile video
Aug 2 - Israel's military releases video of a previously classified missile used on conflicts in Lebanon and Gaza. Rough Cut (no reporter narration).
Metro CEO Eckhard Cordes outlines plan to revive sales
Aug 2 - Eckhard Cordes, the chief executive of Metro tells Insider how he plans to revive weak sales at the German retailer after slashing its 2011 outlook.
Breakingviews: Barclays' targets still look a stretch
Aug 2 - Falling bad debts have helped Barclays to meet forecasts for H1. But to achieve its goal for 2013, it will need strong topline growth. A challenge, given euro zone woes and regulatory uncertainty.
NZ murder trial opens in China
Aug 2 - The trial of a Chinese man accused of murdering a taxi driver in New Zealand opens and adjourns in Shanghai. Paul Chapman reports.
Spain and Italy fight to halt runaway yields breaching 7 pct
Aug 2 - WestLB rates strategist Michael Leister analyses whether Spain and Italy can stop their debt yields breaching the psychological 7 percent level from which Greece, Ireland and Portugal never returned.
European shares hit 9-month low
Aug 2 - European shares fell for a third straight session in Tuesday to hit a nine-month low as bleak U.S. manufacturing data raised fresh concerns over the health of the global economy. Sonia Legg reports.
Hungry Somali refugees fast for Ramadan
Aug 2 - Newly arrived Somali refugees at camps in northeastern Kenya's Dadaab begin fasting for Islam's holy month of Ramadan, having fled famine in their homeland. Travis Brecher reports.
More UK QE should be back on agenda, Goodhart says
The Bank of England should now start to debate the need for more quantitative easing to stimulate the UK economy even if no immediate action is needed, former BoE rate setter Charles Goodhart says.
Inside the News: Growth concerns drive risk-off sentiment
An 11th-hour deal to raise the U.S. debt ceiling seems certain, but worries over global growth and euro zone debt contagion spread turbulence through financial markets. Wall Street to open lower.
U.S. default risk fades, downgrade threat looms over markets
IDEAglobal's Michael Gallagher and Foreign & Colonial Investment Trust's Jeremy Tigue talk to Insider about a possible a credit rating downgrade in the U.S. despite the risk not being priced in.
China's knock-off Ikea store
Aug 2 - A Chinese retailer has opened a copycat Ikea furniture store in the latest new wave of piracy sweeping through China. Hayley Platt reports.
Stolen car hits UK cop
Aug. 2 - A Surrey police officer chases after a suspect moments after being hit by the suspects car. Rough Cut (no reporter narration).
Chavez debuts new look
Aug. 2 - Venezuelan President Hugo Chavez debuts a bald head and makes controversial statements in support of Libya's Muammar Gaddafi during a cabinet meeting. Travis Brecher reports
Car bomb in northern Iraq wounds 23
Aug. 2 - A car bomb explodes outside a Christian church in the northern Iraqi city of Kirkuk, wounding at least 23 people. Travis Brecher reports.
Libyan rebels will fight while fasting
Aug. 2 - Libyan rebel fighters on the frontline outside the town of Zlitan say observing Ramadan will not affect their will to fight. Nick Rowlands reports.
Emiratisation: The way forward?
Unrest in the region has accelerated the UAE’s Emeritisation programme. But Emiratis may be struggling with motivation.
149: M&As in China: do politics still speak louder than money?
A couple of foreign M&A's of Chinese companies look likely to go through. Is it just a chip in the Great Wall of protectionism or a real shift in policy? Veteran Chinese executive and entrepreneur Warren Liu discusses the implications for MNCs in China.