This will provide exposure to and training in a widely used molecular docking programme, AutoDock, and will introduce related biomolecular systems of interest. Well-developed tutorials in both AutoDock and Unix will be completed in the first week. The tasks to complete are as follows:
The objective is to give in a nutshell a flavour of the continuity in biological research from design and sample preparation through analysis to functional characterisation. The rotation will be delivered as follows:
Nisin, E234, is an efficient and safe antimicrobial peptide with target-selective action, used worldwide as a food preservative. Resistance and production of nisin in L. lactis is autoregulated via the NisK/R two-component system through a quorum sensing mechanism. NisK is a histidine sensor kinase that is localized in the cytoplasmic membrane and serves as a receptor for nisin. Extracellular nisin activates surface receptor NisK and initiates a signal transduction cascade, which begins with autophosphorylation of NisK. Phosphorylated NisK transfers a phosphoryl group to NisR, which recruits downstream response regulators to nisA and nisF operators, the nisin biosynthesis genes. We seek structural characterisation of the receptor and activated complex in wild type and constitutively phosphorylated engineered NisK alone and in activated complexes with nisin and with downstream signal transducer, NisR. This will be done using advanced computational techniques, NMR, crystallography and circular dichroism.
The project builds on our expertise in lantionine antimicrobials and molecular engineering. The project also aims to engineer nisin-informed constitutive activators of the receptor system for increased antimicrobial production and explore their role as decoys in adjuvant antimicrobial therapies.
The University of NottinghamUniversity Park
Nottingham, NG7 2RD
Tel: +44 (0) 115 8466946
Connect with the University of Nottingham through social media and our blogs.
Campus maps | More contact information | Jobs
Browser does not support script.