Centre for Biomolecular Sciences

Image of Shakib Alhagh

Shakib Alhagh

PhD student, Faculty of Medicine & Health Sciences


  • workRoom C81 Department of Life sciences
    University Park
    NG7 2RD
  • work+44 (0) 115 846 8001
  • fax+44 (0) 115 846 8002


The human race has always been at war with microbes and infectious disease is one of the few genuine adventures left in the world. I have had a long-standing interest in microbial life cycles and their mechanism of action. I obtained my undergraduate degree in General Microbiology and completed a master of science program in Molecular and Clinical Microbiology from the University of Nottingham. My current work activities are molecular diagnosis and pathogenesis of infectious diseases. I practised a wide range of microbiological techniques in the workplace and academia such as Sample preparation, staining, plating, pouring and Pathogen identification. Microbial infections are the primary death cause in humans worldwide. Improvements in advanced diagnosis will be the main focus to prevent, diagnose and treat infectious diseases.My current research project is focused on the molecular pathogenesis and human genetic response to bacterial infections. science strives to harness the potential of nanotechnology and it has become apparent that natural selection has already provided many solutions. Advances in molecular biology and high-resolution imaging have revealed the incredible complexity and efficiency of macromolecular protein machines. Prokaryotes have developed nano-scale devices to answer a range of problems including motility, secretion and delivery of bioactive macromolecules into the environment and other cells. I am very interested in defining the role of bacterial virulence factors in host-pathogen interaction and identifying their host receptors for possible vaccine and novel antibiotics candidates.

Expertise Summary

  • monitor and identify microorganisms
  • track microorganisms in a range of environments
  • monitor and assess samples from a range of sources
  • follow regular sampling schedules within a specific environment
  • use a variety of identification methods, including molecular techniques, to test samples
  • develop new techniques, products and processes
  • develop and plan methods to prevent the spread of disease
  • develop and register new medicines, vaccines, diagnostic tests and pharmaceutical products
  • plan, implement and evaluate new products in clinical trials
  • Processes patient specimens and performs microbiological analyses
  • Gram staining specimen slides for microscopic view and read wet mounts
  • MIC test and confirmation of Microbial Resistance
  • Interprets laboratory findings and completes technical reports
  • Preparation of body fluids, tissues and organs for clinical testing purposes
  • Directs corrective actions to reject or replace unacceptable specimens
  • basic microbiology SOP's and methods
  • manage and oversee laboratory work
  • inoculating Agar Plates
  • inoculating Broths
  • Growth Media
  • serial Dilution
  • plate counts
  • Motility
  • Biochemical Tests

DNA Extraction- Real Time PCR- Multiplex PCR- Inverse PCR- DNA Sequencing - ELISA -Molecular Cloning - Flow cytometry - Bacterial Cell culture - Bacterial Transformation - Western Blotting - SDS-PAGE - Plasmid Isolation - Iron Utilization Assay - Plasmid Purification- Pulse Field Gel Electrophoresis- Gene Digestion and Ligation - Affinity chromatography- Restriction Fragment length Polymorphism (RFLP) - API Strips Rapid Tests - Catalase test- Oxidase Test- Urease test- TSI Agar Test - Fluorescent Antibodies - HIV Immunoblot Test- Plasmid fingerprinting- Bacteriophage Typing- Fluorescence and Confocal Microscopy-DNA Microarray- Ribotyping- Ligase chain reaction- Loop Mediated Isothermal Amplification- SPSS- End note- Activity Tecan Assay

Teaching Summary

Philosophy of Science and Scientific Ethics Mathematics in the lab Introduction to SPSS Regression analysis in STATA Post-genomics and bioinformatics Epidemiological Study… read more

Research Summary

Characterising the role of potential accessory factors that facilitate secretion of the EspC and AaaA autotransporters

Autotransporter proteins are the super-family of bacterial secreted proteins and act as virulence factors that help the bacteria to evade immune system.The autotransporter secreted by Enteropathogenic E. coli, EspC, is localised in a specific manner as it is secreted from EPEC. ThePseudomonas aeruginosa autotransporter virulence factor (AaaA) follows a similar route out of the bacterium. My current research PhD project would use fluorescently tagged versions of EspC and AaaA to follow their localisation as they interact with host cell targets.

  • Philosophy of Science and Scientific Ethics
  • Mathematics in the lab
  • Introduction to SPSS
  • Regression analysis in STATA
  • Post-genomics and bioinformatics
  • Epidemiological Study Design
  • Bacterial Pathogenesis and infections
  • Infection Control and Public Health
  • Viral Pathogenesis and Infections
  • Prevention and Treatment of Infection
  • Clinical Microbiology Research Methods
  • Diagnostic Microbiology and Laboratory Methods
  • Advanced Clinical Microbiology
  • Identification and confirmation of isolate obtained from culture
  • Rapid detection of Antibiotic Resistance
  • Clinical Specimen Preparation (CSP)
  • The history of antibiotics and emergence of antibiotic resistance
  • Antibiotic targets and mechanisms of resistance
  • Diagnostic laboratory identification of AMR
  • Break points and standardization
  • AMR surveillance methods and burden of drug-resistant infections
  • Pharmacokinetics, exposure and drug failure
  • One health and AMR
  • The use of genomics in AMR
  • Antibiotic usage and agriculture
  • AMR and the environment
  • AMR and the microbiome
  • Infection control
  • TB diagnosis
  • Antimicrobial stewardship
  • The role of diagnostics in reducing antibiotic usage
  • Role of water, sanitation and hygiene in AMR
  • Role of vaccines in reducing AMR
  • Novel alternatives to antimicrobials
  • Developing new therapies
  • Economics of AMR
  • Social science aspects of antibiotic use
  • Molecular Cell Biology & Infection
  • Vaccine Immunology
  • Pathogen Genomics
  • Molecular Biology & Recombinant DNA Techniques
  • Medical Terminology for healthcare professionals
  • Cellular and Molecular Biology
  • Systematic Bacteriology
  • Molecular Bacterial Diagnosis
  • Overview of Neglected Tropical Diseases (NTDs)
  • Controlling pandemics
  • Polio
  • Tuberculosis (TB)
  • Human Immunodeficiency Virus (HIV)
  • Malaria
  • Hepatitis
  • Overview of Non-Communicable Diseases
  • Blood pressure and obesity
  • Health, poverty and development
  • Burden of disease
  • Mental health
  • Salt industry
  • Climate change and global health
  • Lead: A global public health issue
  • Air pollution
  • Improving school children's health and nutrition
  • Global maternal health
  • Eye health and health system strengthening
  • Careers in global health
  • Global health in local clinics
  • Humanitarianism
  • Changing global health policy with smart advocacy
  • Disaster response
  • Ethics in mass drug administration

Past Research

Edinburgh Annual Microbiology society Conference 2017

Characterizing the role of a novel operon of N.meningitidis involved in Iron uptake and virulence

Birmingham Annual Microbiology society Conference 2018

Shakib Alhagh, Mahmoud Ashawesh,Stephanie Pomier,Louise Arnold, Christopher Penfold, Kim R Hardie (2018). Characterising the role of potential accessory factors that facilitate secretion of the EspC autotransporter, Nottingham University, UK.

Belfast Annual Microbiology society Conference 2019

Shakib Alhagh,James Brown, Daniella Spencer, Christopher Penfold,Kim R Hardie.Investigating the regulatory role of RpoN upon the Pseudomonas aeruginosa autotransporter AaaA. Nottingham University, UK.

Centre for Biomolecular Sciences

University of Nottingham
University Park
Nottingham, NG7 2RD

telephone: +44 (0) 115 846 8001
fax: +44 (0) 115 846 8002
email: CBS Enquiries