Alistair Hume

Contact

• workRoom D14 Medical School, QMC
  Queen's Medical Centre
  Nottingham
  NG7 2UH
  UK
• work01158230102
• fax01158230142
• Alistair.Hume@nottingham.ac.uk
• http://www.nottingham.ac.uk/biomedsci/people/school-directory/Alistair.Hume

Biography

University of Dundee: BSc Biochemistry,

University of Sussex: DPhil Biochemistry.

Imperial College London: Post-doctoral researcher.

University of Nottingham: Appointed assistant professor in molecular biology 2010-present

Teaching Summary

Convenor of 3^rd year biochemistry research project modules LIFE3042 and LIFE3081

Co-convenor of 4^th year MSci research project module LIFE4108/9

Convenor of the 3^rd year Life History of Proteins module LIFE3093

Senior Tutor for biochemistry degrees

Chair of the biochemistry student-staff-forum

Research Summary

Cells are fundamental units of the body and their correct function is vital for our health and survival. My research focuses on understanding how cells organise the complex array of internal… read more

Selected Publications

• OSTROWSKI, M., CARMO, N.B., KRUMEICH, S., FANGET, I., RAPOSO, G., SAVINA, A., MOITA, C.F., SCHAUER, K., HUME, A.N., FREITAS, R.P., GOUD, B., BENAROCH, P., HACOHEN, N., FUKUDA, M. and DESNOS, C., 2010. Rab27a and Rab27b control different steps of the exosome secretion pathway Nature Cell Biology. 12(1), 19-30
• NIGHTINGALE TD, PATTNI K, HUME AN, SEABRA MC, CUTLER DF., 2009. Rab27a and Myrip regulate the amount and multimeric state of vWF released from endothelial cells. Blood. 113, 5010-8
• HUME, A.N., BUTTGEREIT, J., AL-AWADHI, A,M., AL-SUWAIDI, S.S., JOHN, A., BADER, M., SEABRA, M.C., AL-GAZALI, L. and ALI, B.R., 2009. Defective cellular trafficking of missense NPR-B mutants is the major mechanism underlying acromesomelic dysplasia-type Maroteaux Human Molecular Genetics. 18(2), 267-277
• HUME, A.N., WILSON, M.S., USHAKOV, D.S., FERENCZI, M.A. and SEABRA, M.C., 2011. Semi-automated analysis of organelle movement and membrane content: understanding rab-motor complex transport function Traffic. 12(12), 1686-1701

• View all publications

Current Research

Cells are fundamental units of the body and their correct function is vital for our health and survival. My research focuses on understanding how cells organise the complex array of internal structures (organelles) whose activities are vital for their function and how defects in these processes cause disease.

In particular, I am interested in the mechanism by which organelles are transported within the cell and the role played by motor proteins, that move organelles along intracellular protein tracks known as the cytoskeleton, and molecular switches (Rab proteins) that recruit motors to organelles.

Current projects include.

1. understanding the role of Rab GTPases in the pathogenesis of paediatric brain cancer.
2. investigation of the trafficking of protein aggregates in neurodegeneration.
3. understanding how organelles regulate cytoskeleton structure and self-regulate their transport.

I am happy to discuss PhD and MRes and other projects related to the above topics with student candidates interested in those and related areas of research.

• ROBINSON, CHRISTOPHER L., EVANS, RICHARD D., SIVARASA, KAJANA, RAMALHO, JOSE S., BRIGGS, DEBORAH A. and HUME, ALISTAIR N., 2019. The adaptor protein melanophilin regulates dynamic myosin-Va:cargo interaction and dendrite development in melanocytes MOLECULAR BIOLOGY OF THE CELL. 30(6), 742-752
• SANZA, PAOLO, EVANS, RICHARD D., BRIGGS, DEBORAH A., CANTERO, MARTA, MONTOLIU, LLUIS, PATEL, SHYAMAL, SVIDERSKAYA, ELENA V., ITZEN, AYMELT, FIGUEIREDO, ANA C., SEABRA, MIGUEL C. and HUME, ALISTAIR N., 2019. Nucleotide exchange factor Rab3GEP requires DENN and non- DENN elements for activation and targeting of Rab27a JOURNAL OF CELL SCIENCE. 132(9),
• ROBINSON CL, EVANS RD, BRIGGS DA, RAMALHO JS and HUME AN, 2017. Deficiency in kinesin-1 recruitment to melanosomes precludes it from facilitating their centrifugal transport. Journal of cell science. (In Press.)
• OBERHOFER A, SPIELER P, ROSENFELD Y, STEPP WL, CLEETUS A, HUME AN, MUELLER-PLANITZ F and ÖKTEN Z, 2017. Myosin Va's adaptor protein melanophilin enforces track selection on the microtubule and actin networks in vitro. Proceedings of the National Academy of Sciences of the United States of America. (In Press.)
• ROBINSON, CHRISTOPHER L., EVANS, RICHARD D., BRIGGS, DEBORAH A., RAMALHO, JOSE S. and HUME, ALISTAIR N., 2017. Inefficient recruitment of kinesin-1 to melanosomes precludes it from facilitating their transport JOURNAL OF CELL SCIENCE. 130(12), 2056-2065
• PYLYPENKO O, WELZ T, TITTEL J, KOLLMAR M, CHARDON F, MALHERBE G, WEISS S, MICHEL CI, SAMOL-WOLF A, GRASSKAMP AT, HUME A, GOUD B, BARON B, ENGLAND P, TITUS MA, SCHWILLE P, WEIDEMANN T, HOUDUSSE A and KERKHOFF E, 2016. Coordinated recruitment of Spir actin nucleators and myosin V motors to Rab11 vesicle membranes. eLife. 5,
• BOOTH AE, TARAFDER AK, HUME AN, RECCHI C and SEABRA MC, 2016. Correction: A Role for Na+,K+-ATPase α1 in Regulating Rab27a Localisation on Melanosomes. PloS one. 11(2), e0148719
• HUME AN, 2015. Myosin--a monarch of pigment transport? Pigment cell & melanoma research. 28(2), 126-7
• TARAFDER AK, BOLASCO G, CORREIA MS, PEREIRA FJC, IANNONE L, HUME AN, KIRKPATRICK N, PICARDO M, TORRISI MR, RODRIGUES IP, RAMALHO JS, FUTTER CE, BARRAL DC and SEABRA MC, 2014. Rab11b mediates melanin transfer between donor melanocytes and acceptor keratinocytes via coupled exo/endocytosis. The Journal of investigative dermatology. 134(4), 1056-66
• BOOTH AEG, TARAFDER AK, HUME AN, RECCHI C and SEABRA MC, 2014. A Role for Na+,K+-ATPase α1 in Regulating Rab27a Localisation on Melanosomes. PloS one. 9(7), e102851
• EVANS RD, ROBINSON C, BRIGGS DA, TOOTH DJ, RAMALHO JS, CANTERO M, MONTOLIU L, PATEL S, SVIDERSKAYA EV and HUME AN, 2014. Myosin-va and dynamic actin oppose microtubules to drive long-range organelle transport. Current biology : CB. 24(15), 1743-50
• HUME AN and SEABRA MC, 2014. Melanoma goes on a diet to get moving: toning down phagocytic Rab7 expression helps melanoma to metastasise. Pigment cell & melanoma research. (In Press.)
• HUME AN, 2014. Myosin-V - a monarch of pigment transport. Pigment cell & melanoma research. (In Press.)
• SINGH RK, FURZE RC, BIRRELL MA, RANKIN SM, HUME AN and SEABRA MC, 2014. A role for Rab27 in neutrophil chemotaxis and lung recruitment. BMC cell biology. 15, 39
• SINGH, R.K., LIAO, W., TRACEY-WHITE, D., RECCHI, C., TOLMACHOVA, T., RANKIN, S.M., HUME, A.N. and SEABRA, M.C., 2012. Rab27a-mediated protease release regulates neutrophil recruitment by allowing uropod detachment Journal of Cell Science. 125(7), 1652-1656
• HUME AN, JOHN A, AKAWI NA, AL-AWADHI AM, AL-SUWAIDI SS, AL-GAZALI L and ALI BR, 2012. Retention in the endoplasmic reticulum is the underlying mechanism of some hereditary haemorrhagic telangiectasia type 2 alk1 missense mutations. Molecular and cellular biochemistry. (In Press.)
• BOOTH AEG, SEABRA MC and HUME AN, 2012. Rab27a and melanosomes: a model to investigate the membrane targeting of Rabs. Biochemical Society transactions. 40(6), 1383-8
• SINGH RK, MIZUNO K, WASMEIER C, WAVRE-SHAPTON ST, RECCHI C, CATZ SD, FUTTER C, TOLMACHOVA T, HUME AN and SEABRA MC, 2012. Distinct and opposing roles for Rab27a/Mlph/MyoVa and Rab27b/Munc13-4 in mast cell secretion. The FEBS journal. (In Press.)
• TARAFDER, ABUL K, WASMEIER, CHRISTINA, FIGUEIREDO, ANA C, BOOTH, ANTONIA E G, ORIHARA, ASUMI, RAMALHO, JOSE S, HUME, ALISTAIR N and SEABRA, MIGUEL C, 2011. Rab27a targeting to melanosomes requires nucleotide exchange but not effector binding. Traffic (Copenhagen, Denmark). 12(8), 1056-66
• CHIANG, LILIAN, NGO, JULIE, SCHECHTER, JOEL E, KARVAR, SERHAN, TOLMACHOVA, TANYA, SEABRA, MIGUEL C, HUME, ALISTAIR N and HAMM-ALVAREZ, SARAH F, 2011. Rab27b regulates exocytosis of secretory vesicles in acinar epithelial cells from the lacrimal gland. American journal of physiology. Cell physiology. 301(2), C507-21
• HUME, ALISTAIR N and SEABRA, MIGUEL C, 2011. Melanosomes on the move: a model to understand organelle dynamics. Biochemical Society transactions. 39(5), 1191-6
• HUME, A.N., WILSON, M.S., USHAKOV, D.S., FERENCZI, M.A. and SEABRA, M.C., 2011. Semi-automated analysis of organelle movement and membrane content: understanding rab-motor complex transport function Traffic. 12(12), 1686-1701
• BOLASCO, GIULIA, TRACEY-WHITE, DHANI C, TOLMACHOVA, TANYA, THORLEY, ANDREW J, TETLEY, TERESA D, SEABRA, MIGUEL C and HUME, ALISTAIR N, 2011. Loss of Rab27 function results in abnormal lung epithelium structure in mice. American journal of physiology. Cell physiology. 300(3), C466-76
• HENDRIX A and HUME AN, 2011. Exosome Signaling In Mammary Gland Development And Cancer. The International Journal Of Developmental Biology. 55(7-9), 879-87
• HUME, AN and SEABRA, MC, 2011. Melanosomes On The Move: A Model To Understand Organelle Dynamics Biochemical Society Transactions. 39, 1191-1196
• ALI BR, NOUVEL I, LEUNG KF, HUME AN, SEABRA MC., 2010. A novel statin-mediated "prenylation block-and-release" assay provides insight into the membrane targeting mechanisms of small GTPases. Biochem Biophys Res Commun. 397, 34-41
• OSTROWSKI, M., CARMO, N.B., KRUMEICH, S., FANGET, I., RAPOSO, G., SAVINA, A., MOITA, C.F., SCHAUER, K., HUME, A.N., FREITAS, R.P., GOUD, B., BENAROCH, P., HACOHEN, N., FUKUDA, M. and DESNOS, C., 2010. Rab27a and Rab27b control different steps of the exosome secretion pathway Nature Cell Biology. 12(1), 19-30
• RAMALHO JS, LOPES VS, TARAFDER AK, SEABRA MC, HUME AN, 2009. Myosin and Rab linking protein, Myrip, activates MyoVa and MyoVIIa via distinct domains in melanosome transport. Pigment Cell Melanoma Res.. 22, 461-73
• HUME, A.N., BUTTGEREIT, J., AL-AWADHI, A,M., AL-SUWAIDI, S.S., JOHN, A., BADER, M., SEABRA, M.C., AL-GAZALI, L. and ALI, B.R., 2009. Defective cellular trafficking of missense NPR-B mutants is the major mechanism underlying acromesomelic dysplasia-type Maroteaux Human Molecular Genetics. 18(2), 267-277
• NIGHTINGALE TD, PATTNI K, HUME AN, SEABRA MC, CUTLER DF., 2009. Rab27a and Myrip regulate the amount and multimeric state of vWF released from endothelial cells. Blood. 113, 5010-8
• WASMEIER C, HUME AN, BOLASCO G, SEABRA MC., 2008. Melanosomes at a glance. J Cell Sci.. 121, 3995-9
• HUME, AN, USHAKOV, DS, TARAFDER, AK, FERENCZI, MA and SEABRA, MC, 2007. Rab27a And Myova Are The Primary Miph Interactors Regulating Melanosome Transport In Melanocytes Journal Of Cell Science. 120(17), 3111-3122
• HUME AN, TARAFDER AK, RAMALHO JS, SVIDERSKAYA EV, SEABRA MC., 2006. A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes. Mol Biol Cell.. 17, 4720-35
• ONFELT B, NEDVETZKI S, BENNINGER RK, PURBHOO MA, SOWINSKI S, HUME AN, SEABRA MC, NEIL MA, FRENCH PM, DAVIS DM, 2006. Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria. J Immunol.. 177, 8476-83
• HANNAH MJ, HUME AN, ARRIBAS M, WILLIAMS R, HEWLETT LJ, SEABRA MC, CUTLER DF., 2003. Weibel-Palade bodies recruit Rab27 by a content-driven, maturation-dependent mechanism that is independent of cell type. J Cell Sci.. 116, 3939-48
• GOMES AQ, ALI BR, RAMALHO JS, GODFREY RF, BARRAL DC, HUME AN, SEABRA MC., 2003. Membrane targeting of Rab GTPases is influenced by the prenylation motif. Mol Biol Cell.. 14, 1882-99
• LARIJANI B, HUME AN, TARAFDER AK, SEABRA MC., 2003. Multiple factors contribute to inefficient prenylation of Rab27a in Rab prenylation diseases. J Biol Chem.. 278, 46798-804
• STROM M, HUME AN, TARAFDER AK, BARKAGIANNI E, SEABRA MC., 2002. A family of Rab27-binding proteins. Melanophilin links Rab27a and myosin Va function in melanosome transport. J Biol Chem.. 277, 25423-30
• HUME AN, COLLINSON LM, HOPKINS CR, STROM M, BARRAL DC, BOSSI G, GRIFFITHS GM, SEABRA MC., 2002. The leaden gene product is required with Rab27a to recruit myosin Va to melanosomes in melanocytes Traffic. 3, 193-202
• BARRAL DC, RAMALHO JS, ANDERS R, HUME AN, KNAPTON HJ, TOLMACHOVA T, COLLINSON LM, GOULDING D, AUTHI KS, SEABRA MC., 2002. Functional redundancy of Rab27 proteins and the pathogenesis of Griscelli syndrome J Clin Invest.. 110, 247-57
• SEABRA MC, MULES EH, HUME AN., 2002. Rab GTPases, intracellular traffic and disease Trends Mol Med.. 8, 23-30
• PEREIRA-LEAL JB, HUME AN, SEABRA MC., 2001. Prenylation of Rab GTPases: molecular mechanisms and involvement in genetic disease. FEBS Lett.. 498, 197-200
• RAMALHO JS, TOLMACHOVA T, HUME AN, MCGUIGAN A, GREGORY-EVANS CY, HUXLEY C, SEABRA MC., 2001. Chromosomal mapping, gene structure and characterization of the human and murine RAB27B gene. BMC Genet.. 2, 2
• STINCHCOMBE JC, BARRAL DC, MULES EH, BOOTH S, HUME AN, MACHESKY LM, SEABRA MC, GRIFFITHS GM., 2001. Rab27a is required for regulated secretion in cytotoxic T lymphocytes. J Cell Biol.. 152, 825-34
• HUME AN, COLLINSON LM, RAPAK A, GOMES AQ, HOPKINS CR, SEABRA MC., 2001. Rab27a regulates the peripheral distribution of melanosomes in melanocytes. J Cell Biol.. 152, 795-808