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Julie Turzanski

Research Fellow, Faculty of Medicine & Health Sciences

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Biography

My background is in leukaemia and lymphoma bench research, but I have recently moved into health research at Nottingham Clinical Trials Unit.

Expertise Summary

Qualitative Research

In the context of clinical trial methodology research: focus groups, Delphi surveys and consensus meetings for determining key trial site performance metrics.

Systematic Reviews

Laboratory skills

  • Cell culture: leukaemia and lymphoma cells in suspension; adherent tumour cells; preparation, culture, maintenance and screening of hybridoma cells for monoclonal antibody production.
  • Processing of blood samples to extract leucocytes for assays.
  • Preparing cells for storage in liquid nitrogen and their revival.
  • Multi-colour- flow cytometric analysis (using Becton Dickinson FACScan/FACSCalibur and Becton Coulter FC500 machines) for investigating signalling pathways: such as calcium spikes, cell death and cell surface marker expression.
  • Fluorescent and confocal microscopy, including the real-time tracking of the internalisation of fluorescently-labelled cell surface markers.
  • Labelling antibodies with fluorescent markers for detection by flow cytometry and microscopy.
  • Gel electrophoresis, Western blotting and casein zymography.
  • ELISAs.
  • PCR.
  • Immunohistochemistry for investigating the binding of potentially therapeutic monoclonal antibodies to tumour cells.
  • Protein and enzyme assays.
  • Working with radioisotopes.
  • Cell lipid extraction and preparing antigen containing liposomes for triggering immune responses.
  • Assays for investigating cell death and survival.
  • Thin layer chromatography.

Research Summary

Development of a standardised set of metrics for monitoring site performance in multicentre randomised trials: a Delphi study

Site performance is key to the successful delivery of large multicentre randomised trials. Measures of site performance should deliver meaningful, actionable information that can be used to monitor sites and initiate remedial action if necessary. A standardised set of clear and accessible summaries of site performance could facilitate the timely identification and resolution of potential problems, minimising their impact. The aim of this study was to identify and agree a core set of key performance metrics and create a simple reporting tool for managing multicentre trials. This involved a mixed methods approach (focus groups, systematic literature search, Delphi survey and consensus meeting), to identify potential trial site performance metrics and to achieve agreement about the final set. Consensus was reached on a set of eight key perfomance metrics. These have been used to create a simple tool in Microsoft Excel for effective visual reporting of the metrics. This is freely available from the Nottingham Clinical Trials website.

Recent Publications

Past Research

Bench research investigating mechanisms of cell death and survival in Acute Myeloid Leukaemia and non-Hodgkin Lymphoma.

School of Medicine

University of Nottingham
Medical School
Nottingham, NG7 2UH

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