Histotechnology Technical methods

Connective Tissue Stain

Methods Index

Pathology Homepage


1. Bring sections to water

2. Stain in Celestine blue, 3 mins

3. Wash in water

4. Stain in Carazzi Haemalum 3 minutes 5. Wash in water

6. Blue in Scotts tap water

7. Wash in water

8. Rinse in 95% alcohol

9. Stain in Picro-orange 2 mins

10. Wash in water until only red blood cells are yellow

11. Stain in acid fuchsin 5 mins

12. Wash in water

13. Stain in equal parts Picro-orange and 80% alcohol

14. Differentiate in phosphomolybdic acid for 8 mins

15. Wash in water

16. Stain in analine blue, 8 mins

17. Wash in water

18. Dehydrate, clear . Mount sections in DPX


--Fibrin - Bright red

--Muscle - Red

--Collagen - Blue

--Red blood cells - Yellow/Orange


1. 0.2% orange G in saturated alcoholic Picric Acid

2. 1% Acid fuchsin in 3% Trichloroacetic Acid

3. 1% Phosphomolybdic Acid

4. 2% Analine blue W5 in 2% Acetic Acid (soluble Blue)

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk