Histotechnology Technical methods

Stain for Melanin

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1. Sections to distilled water.

2. Treat with silver solution in a coplin jar in a water bath at 45ª for 30 minutes

3 Wash in water.

4. Counterstain in 1% eosin for 30 seconds.

5. Dehydrate, clear . Mount sections in DPX


--Melanin black

--Argentaffin,chromaffin and some lipofuscin black

--Background red



To 20 mls of 10% silver nitrate add concentrated ammonia drop by drop until the formed precipitate almost dissolves to the silver solution add 20 mls of distilled water then filter


Waste ammoniacal silver solution is potentially explosive. Collect all ammoniacal silver and put into the silver waste bottle - it contains saturated sodium chloride to precipitate out the silver, this not only makes it safe but allows the silver to be sold for reclamation. Do not pour waste solutions down the drain.

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk