Histotechnology Technical methods

Laboratory Method

Methods Index

Pathology Homepage


  1. Dissolve 5g of gelatine in 1 litre of distilled water. Do not heat over 60C.

  2. When the gelatine has dissolved add 0.5g of chrome alum (chromic potassium sulphate).

  3. Cool and dip racks of slides or coverslips into the solution.

  4. Drain slides and coverslips onto paper tissue.

  5. Dry overnight in a 37C incubator.


The solution ON USE has to be warm but not hot. It can be kept in a refrigerator overnight and re-warmed on a hot plate so that the method can be repeated.

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk