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1. Fix in methanol 15 minutes.

2. Stain in May-Grunwald 5 minutes.

3. Stain in Giemsa 10 minutes.

4. Rinse in buffer pH 6.8

5. Rinse in 50/50 buffer/acetone.

6. Dehydrate in acetone x2..

7. Clear in xylene x3.

8. Mount sections in DPX

May-Grunwald Working Solution

--190 mls Sorensons pH 6.8 buffer

--25 mls stock May-Grunwald solution

Giemsa Working solution

--190 mls Sorensons pH 6.8 buffer

--25 mls stock Giemsa solution


Staining times and concentrations of working solutions may be adjusted as necessary. The staining solutions should be made up fresh daily.

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk