Histotechnology Technical methods

Stain for Hepatitis B and Copper Associated protein

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1. Sections to water.

2. Treat with acidified potassium permanganate for 3 minutes.

3. Wash in water.

4. Decolourize with 1% oxalic acid.

5. Wash well in water.

6. Stain with shikata orcein for 10-90 minutes.

7. Wash in water.

8. Dehydrate, clear . Mount sections in DPX


--Hepatitis B surface antigen,

--Copper associated protein,

--Elastin fibres purple/brown.



--70% ethanol 100 mls

--Conc. hydrochloric acid 1 ml

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk