Histotechnology Technical methods

Connective tissue Stain

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1. Bring sections to water.

2. Celestine blue 3 mins.

3. Wash in water.

4. Alum haematoxylin 3 mins.

5. Wash in water, blue.

6. Differentiate in 1% acid alcohol.

7. Wash in water, blue.

8. Stain in Van Gieson's solution 5 mins.

9. Dehydrate, clear . Mount sections in DPX


Collagen - red

Nuclei - blue-black

Background - yellow


Van Gieson (Unna's variant)

--Acid fuchsin 0.25g

--Nitric acid 0.5ml

--Glycerin 10.0ml

--Picric acid to saturation

--Distilled water 90ml

NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.

© Copyright 1997 University of Nottingham Medical School Division of Histopathology. This page was last built on Mon, May 5, 1997 with Frontier. Thanks for looking in. Comments to James.Lowe@nottingham.ac.uk