Dr Deepika started as a Research Fellow at School of Pharmacy, University of Nottingham, in September 2020. She is involved in Versus Arthritis project to understand the role of pro- anti-inflammatory lipid mediators in Osteoarthritis pain. She worked as a postdoctoral research assistant at MRC-PPU Dundee for two years, where she used quantitative proteomic approaches to study ubiquitin signalling. Dr. Deepika obtained her PhD in 2011, from Institute of Microbial Technology (IMTECH), Chandigarh, India.
In her doctoral and post-doctoral training Dr. Deepika handled a range of instruments like Orbitrap Fusion Lumos Tribrid, Orbitrap Fusion Tribrid, Thermo LTQ OrbiTrap VelosPro, QExactive HF-X, MALDI-TOF (AB SCIEX TOF/TOF™ 5800) and Agilent 6550 iFunnel Q-TOF LC/MS. She has expertise in MS sample preparation, enrichment, fractionation, data acquisition and data analysis. She has used different techniques like peptide mass fingerprinting, analysis of post-translational modifications (acetylation, ubiquitination), selected reaction monitoring, label-free quantitation, SILAC and TMT labelling for identifying interacting proteins, substrates of enzymes, determining enzyme kinetics and assay development.
Dr Deepika is currently working in a research project funded by Versus Arthritis grant. The project involves MS based targeted lipidomics of pre-clinical and clinical samples to identify pro- and… read more
Dr Deepika is currently working in a research project funded by Versus Arthritis grant. The project involves MS based targeted lipidomics of pre-clinical and clinical samples to identify pro- and anti-inflammtory mediators of pain in Osteoarthritis.
Dr Pathak's PhD was aimed at understanding the role of a relatively understudied post-translational modification, N-alpha acetylation in Mycobacterium tuberculosis. To identify the substrates of N-alpha acetyltransferase, she used, in-vitro MALDI based enzyme assays for identifying the peptides that are getting modified an also the site of modification. To determine the kinetic parameters of the enzyme, she developed a mass spec based method, Selected ion monitoring of peptides, to precisely calculate the Km and Vmax of the enzyme.
Her work as a postdoctoral researcher at MRC-PPU Dundee involved, supporting mass spectrometry based projects in the lab especially understanding the biological role of Deubiquitinases (DUBs) by identification of potential substrates and interacting partners, using quantitative proteomic approaches (LFQ, SILAC and TMT).