Jugal is a qualified and practicing pharmacist having completed an MPharm degree at the UCL School of Pharmacy, graduating with the Dean's List award for academic excellence in the faculty of Life Sciences.
Jugal's pre-registration year was split between hospital and industrial settings, with the former bolstering clinical knowledge and medicinal implementation amongst patients. An industry placement with Glaxosmithkline (Ware, UK) saw Jugal working in the respiratory supply chain on the ELLIPTA product portfolio, investigating bulk and flow properties of active powder blends. This was followed by a summer placement in Piramal Pharmaceuticals (Indore, India), one of India's largest CDMO generic manufacturers, working on the ophthalmic dose form supply chain. Collectively, these experiences increased familiarity with GMP implementation, marketing regulations and commercial KPIs of the pharmaceutical industry.
In September 2016, Jugal joined the CDT in Advanced Therapeutics and Nanomedicines, completing two training projects in year 1. Under the supervision of Dr. Gareth Williams, the first project looked to isolate and characterise exosomes from serum using differential centrifugation, followed by exosome formulation through spray drying for pulmonary inhalation. A second project was conducted as a visiting researcher with Pfizer (Sandwich, UK), looking to optimise the rate of primary and secondary drying during lyophilisation of parenteral formulations. Key techniques used included; thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), Karl Fischer analysis, lyophilisation, freeze drying microscopy (FDM), powder x-ray diffraction (XRPD), specific surface area (SSA) and mass spectrometry.
PhD research Summary
Project title: Bio-sensing exosomal proteins for medical diagnostics.
Supervisors: Dr. Gareth Williams, Professor Stefan Guldin, Professor Kirsten Harvey, Professor Mark Lowdell, Ms. Mei Wong,
Industrial sponsor: Pfizer
Project summary: Exosomes are endocytic, lipid membrane bound supra-molecular structures, sized between 30-150nm. Recent findings indicate exosomal involvement in disease propagation, and that their proteomic cargo could be a source of clinically relevant biomarkers. Consequently, there is a growing demand for translational analytical techniques to characterise exosomal molecular composition and absolute concentration in bodily fluids. This work looks to successfully isolate exosomes from complex biological matrices, discover novel protein biomarkers in Alzheimer's disease (AD) models, and develop a bio-sensing platform specific to the identified marker. Ultimately, the aim is to create a minimally invasive diagnostic assay for AD, enabling early therapeutic intervention.
Xu, Y., Suthar, J., Egbu, R., Weston, A. J., Fogg, A. M., & Williams, G. R. (2018). Reverse microemulsion synthesis of layered gadolinium hydroxide nanoparticles. Journal of Solid State Chemistry, 258, 320-327. https://doi.org/10.1016/j.jssc.2017.10.031