RC3: Advanced Materials for 3D Stem Cell Differentiation and Regenerative Medicine
In this RC we are developing a new concept in studying mammalian cell-material interactions that vastly increases the number of combinations and range of properties that can be quantitatively assessed.
Culture will be explored initially in particulate 2.5D and then ChemoTopo Chip con gurations (from RC1) with a view to move towards 3D culture of stem cell populations and assessing the influence of surface topography, chemistry and elasticity on mesenchymal stem cell differentiation to bone and induced pluripotent stem cell derived cardioprogenitor cell maturation to functional cardiomyocytes.
The first year has seen the successful transfer of methodologies from the Needham laboratories to Nottingham to produce dimpled microparticles, providing an addition to our expertise in producing smooth microparticles. Further methodologies are now being explored in year two such as Pickering emulsion technologies and through the use of different surfactants in collaboration with Derek Irvine in RC1.
Chemistries from earlier work (Patel 2015) have been identified as starting chemistries of interest and surface functionalisation of the microparticles to enhance surface reactivity has been achieved.
A further focus for year two is the production of both smooth and textured microparticles modified with these surface chemistries and new chemistries as identified in other work.