RC4: Advanced Materials for Medical Devices
In this section of the project we are engaging in the fight against antibiotic resistance by identifying next generation biomaterials for medical devices that prevent medical device-associated infections. We will also address the problem of device failure mediated by inflammation arising from undesirable host immune responses of the body to foreign materials.
To test the host immune response to biomaterials, human monocyte derived macrophages are used to provide a marker for inflammatory or regenerative immune responses. Methods for the isolation of human monocytes, their maturation to macrophages and subsequent polarization to the M1 (pro-inflammatory) and M2 (anti- inflammatory) phenotypes have been established. Potential markers for the identification of M1/M2 phenotypes have been tested and suitable ones identified.
Bacterial strains to be used throughout this research theme have been constructed. Methods for testing bacterial attachment and biofilm formation on different biomaterial surfaces and topographies have been successfully established.
Researchers of RC4 in collaboration with the Advanced Microscopy Unit (AMU) at the University of Nottingham are implementing an automated method for imaging micro patterned surfaces in TopoChip format.
The aim is to identify surface topography hits with distinct macrophage polarisation (using the M1 and M2 markers already selected) profile and antibacterial attachment properties in an HT manner.
Markers for the identification of macrophages M1/M2 phenotypes and constructed bacterial strains will also be used for surface topographies and chemistries screening in 3D (micro particles).