Stephan Heeb

Contact

• workRoom C94 Centre for Biomolecular Sciences
  University Park
  Nottingham
  NG7 2RD
  UK
• work0115 95 13042
• fax0115 84 68004
• stephan.heeb@nottingham.ac.uk
• https://www.nottingham.ac.uk/cbs/people/stephan.heeb

Selected Publications

• MOSCOSO, J.A., MIKKELSEN, H., HEEB, S., WILLIAMS, P. and FILLOUX, A., 2011. The Pseudomonas aeruginosa sensor RetS switches Type III and Type VI secretion via c-di-GMP signalling Environmental Microbiology. 13(12), 3128-3138
• GONZÁLEZ, N., HEEB, S., VALVERDE, C., KAY, E., REIMMANN, C., JUNIER, T. and HAAS, D., 2008. Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species BMC Genomics. 9, 167
• RAMSAY, J.P., SULLIVAN, J.T., JAMBARI, N., ORTORI, C.A., HEEB, S., WILLIAMS, P., BARRETT, D.A., LAMONT, I.L. and RONSON, C.W., 2009. A LuxRI-family regulatory system controls excision and transfer of the Mesorhizobium loti strain R7A symbiosis island by activating expression of two conserved hypothetical genes Molecular Microbiology. 73(6), 1141-1155
• KLOCKGETHER J, MUNDER A, NEUGEBAUER J, DAVENPORT CF, STANKE F, LARBIG KD, HEEB S, SCHÖCK U, POHL TM, WIEHLMANN L and TÜMMLER B, 2010. Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains. Journal Of Bacteriology. 192(4), 1113-21

• View all publications

• SOUKARIEH, F., OTON, E.V., DUBERN, J.-F., GOMES, J., HALLIDAY, N., DE PILAR CRESPO, M., RAMÍREZ-PRADA, J., INSUASTY, B., ABONIA, R., QUIROGA, J., HEEB, S., WILLIAMS, P., STOCKS, M.J. and CÁMARA, M., 2018. In silico and in vitro-guided identification of inhibitors of alkylquinolone-dependent quorum sensing in pseudomonas aeruginosa: Molecules Molecules. 23,
• LÓPEZ-LARA IM, NOGALES J, PECH-CANUL Á, CALATRAVA-MORALES N, BERNABÉU-RODA LM, DURÁN P, CUÉLLAR V, OLIVARES J, ALVAREZ L, PALENZUELA-BRETONES D, ROMERO M, HEEB S, CÁMARA M, GEIGER O and SOTO MJ, 2018. 2-Tridecanone impacts surface-associated bacterial behaviours and hinders plant-bacteria interactions. Environmental microbiology. (In Press.)
• LIU X, YU X, YANG Y, HEEB S, GAO S, CHAN KG, CÁMARA M and GAO K, 2018. Functional identification of the prnABCD operon and its regulation in Serratia plymuthica. Applied microbiology and biotechnology. (In Press.)
• ROMERO M, SILISTRE H, LOVELOCK L, WRIGHT VJ, CHAN KG, HONG KW, WILLIAMS P, CÁMARA M and HEEB S, 2018. Genome-wide mapping of the RNA targets of the Pseudomonas aeruginosa riboregulatory protein RsmN. Nucleic acids research. (In Press.)
• HIGGINS, STEVEN, HEEB, STEPHAN, RAMPIONI, GIORDANO, FLETCHER, MATHEW P., WILLIAMS, PAUL and CAMARA, MIGUEL, 2018. Differential Regulation of the Phenazine Biosynthetic Operons by Quorum Sensing in Pseudomonas aeruginosa PAO1-N FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY. 8,
• SOUKARIEH, FADI, OTON, EDUARD VICO, DUBERN, JEAN-FREDERIC, GOMES, JANICE, HALLIDAY, NIGEL, DE PILAR CRESPO, MARIA, RAMIREZ-PRADA, JONATHAN, INSUASTY, BRAULIO, ABONIA, RODRIGO, QUIROGA, JAIRO, HEEB, STEPHAN, WILLIAMS, PAUL, STOCKS, MICHAEL J. and CAMARA, MIGUEL, 2018. In Silico and in Vitro-Guided Identification of Inhibitors of Alkylquinolone-Dependent Quorum Sensing in Pseudomonas aeruginosa MOLECULES. 23(2),
• NAYLOR, JONATHAN, FELLERMANN, HAROLD, DING, YUCHUN, MOHAMMED, WALEED K., JAKUBOVICS, NICHOLAS S., DAFHNIS-CALAS, FELIX, HEEB, STEPHAN, CAMARA, MIGUEL, MUKHERJEE, JOY, BIGGS, CATHERINE A., WRIGHT, PHILLIP C. and KRASNOGOR, NATALIO, 2018. Simbiotics: A Multiscale Integrative Platform for 3D Modeling of Bacterial Populations (vol 6, pg 1194, 2017) ACS SYNTHETIC BIOLOGY. 7(3), 952-952
• DOBERENZ S, ECKWEILER D, REICHERT O, JENSEN V, BUNK B, SPRÖER C, KORDES A, FRANGIPANI E, LUONG K, KORLACH J, HEEB S, OVERMANN J and KAEVER V, 2017. Identification of a Pseudomonas aeruginosa PAO1 DNA Methyltransferase, Its Targets, and Physiological Roles MBio. 8(1), e02312-16
• HUERTAS-ROSALES Ó, ROMERO M, HEEB S, ESPINOSA-URGEL M, CÁMARA M and RAMOS-GONZÁLEZ MI, 2017. The Pseudomonas putida CsrA/RsmA homologues negatively affect c-di-GMP pools and biofilm formation through the GGDEF/EAL response regulator CfcR. Environmental microbiology. 19(9), 3551-3566
• HARRISON F, MCNALLY A, DA SILVA AC, HEEB S and DIGGLE SP, 2017. Optimised chronic infection models demonstrate that siderophore 'cheating' in Pseudomonas aeruginosa is context specific. The ISME journal. (In Press.)
• RYAN, ROBERT P., FOUHY, YVONNE, LUCEY, JEAN F., CROSSMAN, LISA C., SPIRO, STEPHEN, HE, YA-WEN, ZHANG, LIAN-HUI, HEEB, STEPHAN, CAMARA, MIGUEL, WILLIAMS, PAUL and DOW, J. MAXWELL, 2017. Cell-cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic diGMP turnover (Retraction of Vol 103, Pg 6712, 2006) PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 114(33), E7031-E7031
• KRZYŻANOWSKA DM, OSSOWICKI A, RAJEWSKA M, MACIĄG T, JABŁOŃSKA M, OBUCHOWSKI M, HEEB S and JAFRA S, 2016. When Genome-Based Approach Meets the "Old but Good": Revealing Genes Involved in the Antibacterial Activity of Pseudomonas sp. P482 against Soft Rot Pathogens. Frontiers in microbiology. 7, 782
• MILLER CL, ROMERO M, KARNA SL, CHEN T, HEEB S and LEUNG KP, 2016. RsmW, Pseudomonas aeruginosa small non-coding RsmA-binding RNA upregulated in biofilm versus planktonic growth conditions. BMC microbiology. 16(1), 155
• RAMPIONI G, FALCONE M, HEEB S, FRANGIPANI E, FLETCHER MP, DUBERN JF, VISCA P, LEONI L, CÁMARA M and WILLIAMS P, 2016. Unravelling the Genome-Wide Contributions of Specific 2-Alkyl-4-Quinolones and PqsE to Quorum Sensing in Pseudomonas aeruginosa. PLoS pathogens. 12(11), e1006029
• SINGH S, BARR H, LIU Y, ROBINS A, HEEB S, WILLIAMS P, FOGARTY A, CÁMARA M and MARTÍNEZ-POMARES L, 2015. Granulocyte-Macrophage Colony Stimulatory Factor Enhances the Pro-Inflammatory Response of Interferon-γ-Treated Macrophages to Pseudomonas aeruginosa Infection. PloS one. 10(2), e0117447
• SOH EY, CHHABRA SR, HALLIDAY N, HEEB S, MÜLLER C, BIRMES FS, FETZNER S, CÁMARA M, CHAN K and WILLIAMS P, 2015. Biotic inactivation of the Pseudomonas aeruginosa quinolone signal molecule. Environmental microbiology. 17(11), 4352-4365
• FRESCHI L, JEUKENS J, KUKAVICA-IBRULJ I, BOYLE B, DUPONT MJ, LAROCHE J, LAROSE S, MAAROUFI H, FOTHERGILL JL, MOORE M, WINSOR GL, AARON SD, BARBEAU J, BELL SC, BURNS JL, CAMARA M, CANTIN A, CHARETTE SJ, DEWAR K, DÉZIEL É, GRIMWOOD K, HANCOCK RE, HARRISON JJ, HEEB S, JELSBAK L, JIA B, KENNA DT, KIDD TJ, KLOCKGETHER J, LAM JS, LAMONT IL, LEWENZA S, LOMAN N, MALOUIN F, MANOS J, MCARTHUR AG, MCKEOWN J, MILOT J, NAGHRA H, NGUYEN D, PEREIRA SK, PERRON GG, PIRNAY JP, RAINEY PB, ROUSSEAU S, SANTOS PM, STEPHENSON A, TAYLOR V, TURTON JF, WAGLECHNER N, WILLIAMS P, THRANE SW, WRIGHT GD, BRINKMAN FS, TUCKER NP, TÜMMLER B, WINSTANLEY C and LEVESQUE RC, 2015. Clinical utilization of genomics data produced by the international Pseudomonas aeruginosa consortium. Frontiers in microbiology. 6, 1036
• BEN YEHEZKEL T, RIVAL A, RAZ O, COHEN R, MARX Z, CAMARA M, DUBERN JF, KOCH B, HEEB S, KRASNOGOR N, DELATTRE C and SHAPIRO E, 2015. Synthesis and cell-free cloning of DNA libraries using programmable microfluidics. Nucleic acids research. 44(4), e35
• KULKARNI PR, JIA T, KUEHNE SA, KERKERING TM, MORRIS ER, SEARLE MS, HEEB S, RAO J and KULKARNI RV, 2014. A sequence-based approach for prediction of CsrA/RsmA targets in bacteria with experimental validation in Pseudomonas aeruginosa. Nucleic acids research. 42(11), 6811-25
• ILANGOVAN, A., FLETCHER, M., RAMPIONI, G., PUSTELNY, C., RUMBAUGH, K., HEEB, S., CÁMARA, M, TRUMAN, A., CHHABRA, S.R., EMSLEY, J. and WILLIAMS, P., 2013. Structural basis for native agonist and synthetic inhibitor recognition by the Pseudomonas aeruginosa quorum sensing regulator PqsR (MvfR) PLoS Pathogens. 9(7), e1003508
• MORRIS, E.R., HALL, G., LI, C., HEEB, S., KULKARNI, R.V., LOVELOCK, L., SILISTRE, H., MESSINA, M., CÁMARA, M., EMSLEY, J., WILLIAMS, P. and SEARLE, M.S., 2013. Structural rearrangement in an RsmA/CsrA ortholog of Pseudomonas aeruginosa creates a dimeric RNA-binding protein, RsmN Structure. 21(9), 1659-1671
• FRANGIPANI E, VISAGGIO D, HEEB S, KAEVER V, CÁMARA M, VISCA P and IMPERI F, 2013. The Gac/Rsm and cyclic-di-GMP signalling networks coordinately regulate iron uptake in Pseudomonas aeruginosa. Environmental microbiology. 16, 676–688
• LUCKETT, J.C.A., DARCH, O., WATTERS, C., ABUOUN, M., WRIGHT, V., PAREDES-OSSES, E., WARD, J., GOTO, H., HEEB, S., POMMIER, S., RUMBAUGH, K.P., CÁMARA, M. and HARDIE, K.R., 2012. A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity PLoS Pathogens. 8(8), e1002854
• KONG HS, ROBERTS DP, PATTERSON CD, KUEHNE SA, HEEB S, LAKSHMAN DK and LYDON J, 2012. Effect of overexpressing rsma from pseudomonas aeruginosa on virulence of select phytotoxin-producing strains of p. Syringae. Phytopathology. 102(6), 575-87
• FERNÁNDEZ-PIÑAR R, ESPINOSA-URGEL M, DUBERN J, HEEB S, RAMOS JL and CÁMARA M, 2012. Fatty acid-mediated signalling between two Pseudomonas species. Environmental microbiology reports. 4(4), 417-23
• HEEB S, FLETCHER MP, CHHABRA SR, DIGGLE SP, WILLIAMS P and CÁMARA M, 2011. Quinolones: from antibiotics to autoinducers. FEMS microbiology reviews. 35(2), 247-74
• FERNANDEZ-PINAR R, CAMARA M, SORIANO M. I, DUBERN J-F, HEEB S, RAMOS J. L and ESPINOSA-URGEL M., 2011. PpoR, an orphan LuxR-family protein of Pseudomonas putida KT2440, modulates competitive fitness and surface motility independently of N-acylhomoserine lactones Environmental Microbiology Reports. 3(1), 79-85
• MOSCOSO, J.A., MIKKELSEN, H., HEEB, S., WILLIAMS, P. and FILLOUX, A., 2011. The Pseudomonas aeruginosa sensor RetS switches Type III and Type VI secretion via c-di-GMP signalling Environmental Microbiology. 13(12), 3128-3138
• SONNLEITNER, E., GONZALEZ, N., SORGER-DOMENIGG, T., HEEB, S., RICHTER, A.S., BACKOFEN, R., WILLIAMS, P., HÜTTENHOFER, A., HAAS, D. and BLÄSI, U., 2011. The small RNA PhrS stimulates synthesis of the Pseudomonas aeruginosa quinolone signal Molecular Microbiology. 80(4), 868-885
• RAMPIONI, G., PUSTELNY, C., FLETCHER, M.P., WRIGHT, V.J., BRUCE, M., RUMBAUGH, K.P., HEEB, S., CÁMARA, M. and WILLIAMS, P., 2010. Transcriptomic analysis reveals a global alkyl-quinolone-independent regulatory role for PqsE in facilitating the environmental adaptation of Pseudomonas aeruginosa to plant and animal hosts Environmental Microbiology. 12(6), 1659-1673
• CAO H, ROMERO-CAMPERO FJ, HEEB S, CÁMARA M and KRASNOGOR N, 2010. Evolving cell models for systems and synthetic biology. Systems And Synthetic Biology. 4(1), 55-84
• KLOCKGETHER J, MUNDER A, NEUGEBAUER J, DAVENPORT CF, STANKE F, LARBIG KD, HEEB S, SCHÖCK U, POHL TM, WIEHLMANN L and TÜMMLER B, 2010. Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains. Journal Of Bacteriology. 192(4), 1113-21
• KOCH, G, JIMENEZ, PN, MUNTENDAM, R, CHEN, YX, PAPAIOANNOU, E, HEEB, S, CAMARA, M, WILLIAMS, P, COOL, RH and QUAX, WJ, 2010. The Acylase Pvdq Has A Conserved Function Among Fluorescent Pseudomonas Spp. Environmental Microbiology Reports. 2(3), 433-439
• RAMSAY, J.P., SULLIVAN, J.T., JAMBARI, N., ORTORI, C.A., HEEB, S., WILLIAMS, P., BARRETT, D.A., LAMONT, I.L. and RONSON, C.W., 2009. A LuxRI-family regulatory system controls excision and transfer of the Mesorhizobium loti strain R7A symbiosis island by activating expression of two conserved hypothetical genes Molecular Microbiology. 73(6), 1141-1155
• GONZÁLEZ, N., HEEB, S., VALVERDE, C., KAY, E., REIMMANN, C., JUNIER, T. and HAAS, D., 2008. Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species BMC Genomics. 9, 167
• ROMERO M, DIGGLE SP, HEEB S, CÁMARA M and OTERO A, 2008. Quorum quenching activity in Anabaena sp. PCC 7120: identification of AiiC, a novel AHL-acylase. FEMS microbiology letters. 280(1), 73-80
• FOX A, HAAS D, REIMMANN C, HEEB S, FILLOUX A and VOULHOUX R, 2008. Emergence of secretion-defective sublines of Pseudomonas aeruginosa PAO1 resulting from spontaneous mutations in the Vfr gobal regulatory gene. Applied And Environmental Microbiology. 74(6), 1902-8
• HEEB S, KUEHNE SA, BYCROFT M, CRIVII S, ALLEN MD, HAAS D, CÁMARA M and WILLIAMS P, 2006. Functional analysis of the post-transcriptional regulator RsmA reveals a novel RNA-binding site Journal of Molecular Biology. 355(5), 1026-1036
• RYAN RP, FOUHY Y, LUCEY JF, CROSSMAN LC, SPIRO S, HE Y, ZHANG L, HEEB S, CÁMARA M, WILLIAMS P and DOW JM, 2006. Cell-cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover. Proceedings Of The National Academy Of Sciences Of The United States Of America. 103(17), 6712-7
• SIDDIQUI IA, HAAS D and HEEB S, 2005. Extracellular protease of Pseudomonas fluorescens CHA0, a biocontrol factor with activity against the root-knot nematode Meloidogyne incognita. Applied And Environmental Microbiology. 71(9), 5646-9
• HEEB S, VALVERDE C, GIGOT-BONNEFOY C and HAAS D, 2005. Role of the stress sigma factor RpoS in GacA/RsmA-controlled secondary metabolism and resistance to oxidative stress in Pseudomonas fluorescens CHA0. FEMS Microbiology Letters. 243(1), 251-8
• HEEB S, HEURLIER K, VALVERDE C, CÁMARA M, HAAS D and WILLIAMS P, 2004. Post-transcriptional regulation in Pseudomonas spp. via the Gac/Rsm regulatory network. In: RAMOS, J.L., ed., Pseudomonas: Virulence and gene regulation Vol. 2. Kluwer Academic. 239-256
• HEURLIER K, WILLIAMS F, HEEB S, DORMOND C, PESSI G, SINGER D, CÁMARA M, WILLIAMS P and HAAS D, 2004. Positive control of swarming, rhamnolipid synthesis, and lipase production by the posttranscriptional RsmA/RsmZ system in Pseudomonas aeruginosa PAO1. Journal Of Bacteriology. 186(10), 2936-45
• VALVERDE C, HEEB S, KEEL C and HAAS D, 2003. RsmY, a small regulatory RNA, is required in concert with RsmZ for GacA-dependent expression of biocontrol traits in Pseudomonas fluorescens CHA0. Molecular Microbiology. 50(4), 1361-79
• ZUBER S, CARRUTHERS F, KEEL C, MATTART A, BLUMER C, PESSI G, GIGOT-BONNEFOY C, SCHNIDER-KEEL U, HEEB S, REIMMANN C and HAAS D, 2003. GacS sensor domains pertinent to the regulation of exoproduct formation and to the biocontrol potential of Pseudomonas fluorescens CHA0. Molecular Plant-Microbe Interactions. 16(7), 634-44
• HEEB S, BLUMER C and HAAS D, 2002. Regulatory RNA as mediator in GacA/RsmA-dependent global control of exoproduct formation in Pseudomonas fluorescens CHA0. Journal Of Bacteriology. 184(4), 1046-56
• HEEB S and HAAS D, 2001. Regulatory roles of the GacA/GacA two-component system in plant-associated and other Gram-negative bacteria. Molecular Plant-Microbe Interactions. 14(12), 1351-63
• HEEB S, ITOH Y, NISHIJYO T, SCHNIDER U, KEEL C, WADE J, WALSH U, O'GARA F and HAAS D, 2000. Small, stable shuttle vectors based on the minimal pVS1 replicon for use in Gram-negative, plant-associated bacteria. Molecular Plant-Microbe Interactions. 13(2), 232-7
• BLUMER C, HEEB S, PESSI G and HAAS D, 1999. Global GacA-steered control of cyanide and exoprotease production in Pseudomonas fluorescens involves specific ribosome binding sites. Proceedings Of The National Academy Of Sciences Of The United States Of America. 96(24), 14073-8
• MAURHOFER M, REIMMANN C, SCHMIDLI-SACHERER P, HEEB S, HAAS D and DEFAGO G, 1998. Salicylic acid biosynthetic genes expressed in Pseudomonas fluorescens strain P3 improve the induction of systemic resistance in tobacco against tobacco necrosis virus. Phytopathology. 88(7), 678-684