Wayne Carter

Contact

• workRoom Room 4109 Division of Medical Sciences & Graduate Entry Medicine
  Royal Derby Hospital
  Uttoxeter Road
  Derby
  DE22 3DT
  UK
• work01332 724738
• fax01332 724626
• wayne.carter@nottingham.ac.uk

Biography

Dr Wayne Grant Carter completed a BSc (Hons) degree in Biochemistry with Nutrition and then a PhD in Biochemistry at the University of Southampton. He subsequently undertook post-doctoral research posts at The Babraham Institute, Cambridge; Imperial College, London; University of California at Irvine, USA, and then at the University of Oxford. Additionally, Dr Carter has worked for a global reagent supplier, Sigma, a SME company, Mobious Genomics, and has been employed as a consultant for Syngenta. Dr Carter is currently an Associate Professor and Group Leader (Clinical Toxicology lab) within the School of Medicine, University of Nottingham. Dr Carter's research interests are varied and include detection and utilisation of novel biomarkers of toxicological exposure.

Expertise Summary

Understanding the molecular mechanisms that underpin disease provides the basis for targeted therapeutics. The research in my lab is concerned with understanding protein changes and modifications that can trigger pathology. In recent years we have focussed on hepato- and neuro-toxicological mechanisms. We employ cell and animal models and human postmortem tissue to understand pathological changes and utilize a broad range of biological and biochemical techniques to study disease. Current projects include the biological impact of environmental pesticides and heavy metals, liver and brain toxicity of alcohol, mechanisms that trigger protein aggregation in neurodegenerative diseases, and purification and assessment of the beneficial properties of phytochemicals.

Teaching Summary

University Teaching and Teaching-related Activities:

• Module Convenor and Assessments Lead: Clinical Toxicology Studies (Medicine, BMBS); Cancer Biology (BSc, Medical Physiology & Therapeutics (MPT) degree); Immunology (BSc, MPT degree); Clinical Toxicology (BSc, MPT degree). Cellular Disease (BSc, MPT).
• Module Contributor: Range of BMBS and BSc modules.
• Academic Unit 3, Co-Lead and Derby Homebase Lead: BMedSci program.
• Senior Tutor: BMBS Graduate Entry Medicine and Foundation Medicine and MPT degrees (2010-present).
• Admissions Lead & Widening Participation Lead: MPT degree (2011-present).
• School of Medicine Alumni Lead (2018-present).

Selected Publications

• VIGNESWARA, V., CASS, S., WAYNE, D., BOLT, E.L., RAY, D.E. and CARTER, W.G., 2013. Molecular ageing of alpha- and beta-synucleins: protein damage and repair mechanisms PLoS ONE. 8(4), e61442
• LEGGATE, M., CARTER, W.G., EVANS, M.J.C., VENNARD, R.A., SRIBALA-SUNDARAM, S. and NIMMO, M.A., 2012. Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high-intensity intermittent training in overweight and obese males Journal of Applied Physiology. 112(8), 1353-1360
• TARHONI, M.H., VIGNESWARA, V., SMITH, M., ANDERSON, S., WIGMORE, P., LEES, J.E., RAY, D.E. and CARTER, W.G., 2011. Detection, quantification, and microlocalisation of targets of pesticides using microchannel plate autoradiographic imagers Molecules. 16(10), 8535-8551
• CARTER, W.G., TARHONI, M.H. and RAY, D.E., 2010. Analytical approaches to investigate protein-pesticide adducts Journal of Chromatography B. 878(17-18), 1312-1319

• View all publications

• EL SHARAZLY BM, ABOUL ASAAD IA, YASSEN NA, EL MAGHRABY GM, CARTER WG, MOHAMED DA, AMER BS and ISMAIL HIH, 2023. Mefloquine loaded niosomes as a promising approach for the treatment of acute and chronic toxoplasmosis. Acta Tropica. 239, 106810
• ALNASSER MN, ALSAADI AM, WHITBY A, KIM D-H, MELLOR IR and CARTER WG, 2023. Acai Berry (Euterpe sp) extracts are neuroprotective against L-Glutamate-induced toxicity by limiting mitochondrial dysfunction and cellular redox stress. Life. 13(4), 1019
• MUDYANSELAGE AW, WIJAMUNIGE BC, KOCON A and CARTER WG, 2023. Differentiated neurons are more vulnerable to organophosphate and carbamate neurotoxicity than undifferentiated neurons due to the induction of redox stress and accumulate oxidatively-damaged proteins. Brain Sciences. 13, 728
• AMAT-UR-RASOOL H, AHMED M, HASNAIN S, AHMED A and CARTER WG, 2022. In Silico Design of Dual-Binding Site Anti-Cholinesterase Phytochemical Heterodimers as Treatment Options for Alzheimer’s Disease. Current Issues in Molecular Biology.. 44(1), 152-175
• AMAT-UR-RASOOL H, LATIF A, YASMEEN A, SHAHID N, AZAM S, BASHIR A, HUSNAIN T, RAO AQ, CARTER WG and SHAHID AA, 2022. Enhanced expression of plasma membrane intrinsic protein 2 improves cotton fiber length in Gossypium arboretum. Molecular Biology Reports.
• ALSAADI A and CARTER WG, 2022. Analysis of illicit drugs and wastewater-based epidemiology: current methodological techniques European Pharmaceutical Review. 27(2), 2-4
• AL-NASSER M, MELLOR I and CARTER WG, 2022. Is L-Glutamate Toxic to Neurons and Thereby Contributes to Neuronal Loss and Neurodegeneration? A Systematic Review Brain Sciences. 12(5), 577
• ALI UA, IFTIKHAR N, AMAT-UR-RASOOL H, AHMED M, HAFEEZ J and CARTER WG, 2022. The Psychological Impact of Coronavirus Disease 2019 on Patients Attending a Tertiary Healthcare Facility in Pakistan: A Cross-Sectional Study. Healthcare. 2022(10), 1049
• ELMORSY E, AL-GHAFARI A, AL DOGHAITHER H, SALAMA M and CARTER WG, 2022. An Investigation of the Neurotoxic Effects of Malathion, Chlorpyrifos, and Paraquat to Different Brain Regions Brain Sciences. 12(8), 975
• ALNASSER MN, MELLOR IR and CARTER WG, 2022. A Preliminary Assessment of the Nutraceutical Potential of Acai Berry (Euterpe sp.) as a Potential Natural Treatment for Alzheimer’s Disease Molecules. 27(15), 4891
• NASEEM A, RASOOL F, AHMED A and CARTER WG, 2022. The Potential of Stilbene Compounds to Inhibit Mpro Protease as a Natural Treatment Strategy for Coronavirus Disease-2019. Current Issues in Molecular Biology.. 45(1), 12-32
• AMAT-UR-RASOOL, H., AHMED, M., HASNAIN, S. and CARTER, W.G., 2021. Anti-Cholinesterase Combination Drug Therapy as a Potential Treatment for Alzheimer’s Disease Brain Sciences. 11, 184
• PRAKASH, S and CARTER WG, 2021. The neuroprotective effects of cannabis-derived phytocannabinoids and resveratrol in Parkinson’s Disease: A systematic literature review of pre-clinical studies. Brain Sciences. 11, 1573
• SCHAFFERT L.-N and CARTER WG, 2021. The importance of protein post-translational modifcations in the pathogenesis and progression of neurodegenerative diseases.. In: R. BAWA and G.F. ANDETTE, eds., Advances in Medical Biochemistry, Genomics, Physiology, and Pathology. Ist Edition. Jenny Stanford Publishing.
• SCHAFFERT, L-N and CARTER, W.G., 2020. Do post-translational modifications influence protein aggregation in neurodegenerative diseases: a systematic review. Brain Sciences. 10(4), 232
• ALELWANI W, ELMORSY E, KATTAN SW, BABTEEN NA, ALNAJEEBI AM, AL-GHAFARI A and CARTER WG, 2020. Carbamazepine induces a bioenergetics disruption to microvascular endothelial cells from the blood-brain barrier. Toxicology letters. 333, 184-191
• AMAT-UR-RASOOL H, SYMES F, TOOTH D, SCHAFFERT L-N, ELMORSY E, AHMED M, HASNAIN S and CARTER WG, 2020. Potential nutraceutical properties of leaves from several commonly cultivated plants Biomolecules. 10, 1556
• CARTER WG, 2020. Dead as a Dodo The Biochemist. 42, 50-50
• NWIDU LL, ALIKWE PCN, ELMORSY E and CARTER WG, 2019. An investigation of potential sources of nutraceuticals from the Niger delta areas, Nigeria for attenuating oxidative stress. Medicines. 6(15),
• AL-GHAFARI A, ELMORSY E, FIKRY E, ALROWAILI M and CARTER WG, 2019. The heavy metals lead and cadmium are cytotoxic to human bone osteoblasts via induction of redox stress. PLoS One. 14(11), e0225341
• DORLING JL, CLAYTON DJ, JONES J, CARTER WG, THACKRAY AE, KING JA, PUCCI A, BATTERHAM RL and STENSEL DJ, 2019. A randomized cross-over trial assessing the effects of acute exercise on appetite, circulating ghrelin concentrations, and butyrylcholinesterase activity in normal-weight males with variants of the obesity-linked FTO rs9939609 polymorphism Am J Clin Nutr. 110(5), 1055-1066
• NWIDU LL, OBOMA YI, ELMORSY E and CARTER WG, 2018. Hepatoprotective effect of hydromethanolic leaf extract of Musanga Cecropioides (Urticaceae) on carbon tetrachloride-induced liver injury and oxidative stress Journal of Taibah University Medical Sciences. 13(4), 344-354
• NWIDU LL, ELMORSY E, APRIOKU JS, SIMINIALAYI I and CARTER WG, 2018. In Vitro Anti-Cholinesterase and Antioxidant Activity of Extracts of Moringa oleifera Plants from Rivers State, Niger Delta, Nigeria Medicines (Basel). 5(3), pii; E71
• NWIDU LL, ELMORSY E and CARTER WG, 2018. In-vitro anti-cholinesterase and anti-oxidant activity of three standardised polyherbal products used for memory enhancing in ethnomedicine of South-East Nigeria Malays J Med Sci. 25(2), 27-39
• NWIDU LL, ELMORSY E, OBOMA YI and CARTER WG, 2018. Hepatoprotective and antioxidant activities of Spondias Mombin leaf and stem extracts against carbon tetrachloride-induced hepatotoxicity Journal of Taibah University Medical Sciences. 13(3), 262-271
• LABISSO WL, RAULIN AC, NWIDU LL, KOCON A, WAYNE D, ERDOZAIN AM, MORENTIN B, SCHWENDENER D, ALLEN G, ENTICOTT J, GERDES HK, JOHNSON L, GRZESKOWIAK J, DRIZOU F, TARBOX R, OSNA NA, KHABANDA KK, CALLADO LF and CARTER WG, 2018. The Loss of α- and β-Tubulin Proteins Are a Pathological Hallmark of Chronic Alcohol Consumption and Natural Brain Ageing Brain Sci. 8(9), pii E175
• NWIDU LL, OBOMA YI, ELMORSY E and CARTER WG, 2018. Alleviation of carbon tetrachloride-induced hepatocellular damage and oxidative stress with a leaf extract of Glyphae brevis (Tiliaceae). Journal of Basic and Clinical Physiology and Pharmacology. 29(6), 609-619
• ELMORSY E, ATTALLA SM, FIKRY E, KOCON A, TURNER R, CHRISTIE D, WARREN A, NWIDU LL and CARTER WG, 2017. Adverse effects of anti-tuberculosis drugs on HepG2 cell bioenergetics Human & Experimental Toxicology. 36, 616-625
• NWIDU LL, ELMORSY E, THORNTON J, WIJAMUNIGE B, WIJESEKARA A, TARBOX R, WARREN A and CARTER WG, 2017. Anti-acetylcholinesterase activity and antioxidant properties of extracts and fractions of Carpolobia lutea Pharmaceutical Biology. 55(1), 1875-1883
• ELMORSY E, AL-GHAFARI A, ALMUTAIRI FM, AGGOUR AM and CARTER WG, 2017. Antidepressants are cytotoxic to rat primary blood brain barrier endothelial cells at high therapeutic concentrations Toxicol In Vitro. 44, 154-163
• CARTER WG, 2017. An answer to Alzheimer’s? Health. 2, 88-89
• OSNA NA, CARTER WG, GANESAN M, KIRPICH IA, MCCLAIN CJ, PETERSEN DR, SHEARN CT and TOMASI ML, 2016. Aberrant post-translational protein modifications in the pathogenesis of alcohol-induced liver injury World J Gastroenterol. 22(27), 6192-6200
• CARTER WG, VIGNESWARA V, NEWLACZYL A, WAYNE D, AHMED B, SADDINGTON S, BREWER C, RAUT N, GERDES HK, ERDOZAIN AM, TOOTH D, BOLT EL, OSNA NA and TUMA DJ, 2015. Isoaspartate, Carbamoyl phosphate synthase-1, and Carbonic anhydrase-III as biomarkers of liver injury. Biochemical and Biophysical Research Communications. 498, 626-631
• ERDOZAIN AM, MORENTIN B, BEDFORD L, KING E, TOOTH D, BREWER C, WAYNE D, JOHNSON L, GERDES HK, WIGMORE P, CALLADO LF and CARTER WG, 2014. Alcohol-related brain damage in humans. PloS one. 9(4), e93586
• VIGNESWARA, V., CASS, S., WAYNE, D., BOLT, E.L., RAY, D.E. and CARTER, W.G., 2013. Molecular ageing of alpha- and beta-synucleins: protein damage and repair mechanisms PLoS ONE. 8(4), e61442
• ALEXANDER SPH, BENSON HE, FACCENDA E, PAWSON AJ, SHARMAN JL, MCGRATH JC, CATTERALL WA, SPEDDING M, PETERS JA, HARMAR AJ, CGTP COLLABORATORS, ABUL-HASN N, ANDERSON CM, ANDERSON CMH, ARAIKSINEN MS, ARITA M, ARTHOFER E, BARKER EL, BARRATT C, BARNES NM, BATHGATE R, BEART PM, BELELLI D, BENNETT AJ, BIRDSALL NJM, BOISON D, BONNER TI, BRAILSFORD L, BRÖER S, BROWN P, CALO G, CARTER WG, CATTERALL WA, CHAN SLF, CHAO MV, CHIANG N, CHRISTOPOULOS A, CHUN JJ, CIDLOWSKI J, CLAPHAM DE, COCKCROFT S, CONNOR MA, COX HM, CUTHBERT A, DAUTZENBERG FM, DAVENPORT AP, DAWSON PA, DENT G, DIJKSTERHUIS JP, DOLLERY CT, DOLPHIN AC, DONOWITZ M, DUBOCOVICH ML, EIDEN L, EIDNE K, EVANS BA, FABBRO D, FAHLKE C, FARNDALE R, FITZGERALD GA, FONG TM, FOWLER CJ, FRY JR, FUNK CD, FUTERMAN AH, GANAPATHY V, GAISNIER B, GERSHENGORN MA, GOLDIN A, GOLDMAN ID, GUNDLACH AL, HAGENBUCH B, HALES TG, HAMMOND JR, HAMON M, HANCOX JC, HAUGER RL, HAY DL, HOBBS AJ, HOLLENBERG MD, HOLLIDAY ND, HOYER D, HYNES NA, INUI K, ISHII S, JACOBSON KA, JARVIS GE, JARVIS MF, JENSEN R, JONES CE, JONES RL, KAIBUCHI K, KANAI Y, KENNEDY C, KERR ID, KHAN AA, KLIENZ MJ, KUKKONEN JP, LAPOINT JY, LEURS R, LINGUEGLIA E, LIPPIAT J, LOLAIT SJ, LUMMIS SCR, LYNCH JW, MACEWAN D, MAGUIRE JJ, MARSHALL IL, MAY JM, MCARDLE CA, MCGRATH JC, MICHEL MC, MILLAR NS, MILLER LJ, MITOLO V, MONK PN, MOORE PK, MOORHOUSE AJ, MOUILLAC B, MURPHY PM, NEUBIG RR, NEUMAIER J, NIESLER B, OBAIDAT A, OFFERMANNS S, OHLSTEIN E, PANARO MA, PARSONS S, PWRTWEE RG, PETERSEN J, PIN J, POYNER DR, PRIGENT S, PROSSNITZ ER, PYNE NJ, PYNE S, QUIGLEY JG, RAMACHANDRAN R, RICHELSON EL, ROBERTS RE, ROSKOSKI R, ROSS RA, ROTH M, RUDNICK G, RYAN RM, SAID SI, SCHILD L, SANGER GJ, SCHOLICH K, SCHOUSBOE A, SCHULTE G, SCHULZ S, SERHAN CN, SEXTON PM, SIBLEY DR, SIEGEL JM, SINGH G, SITSAPESAN R, SMART TG, SMITH DM, SOGA T, STAHL A, STEWART G, STODDART LA, SUMMERS RJ, THORENS B, THWAITES DT, TOLL L, TRAYNOR JR, USDIN TB, VANDENBERG RJ, VILLALON C, VORE M, WALDMAN SA, WARD DT, WILLARS GB, WONNACOTT SJ, WRIGHT E, YE RD, YONEZAWA A and ZIMMERMANN M, 2013. The Concise Guide to PHARMACOLOGY 2013/14: overview. British journal of pharmacology. 170(8), 1449-58
• LEGGATE, M., CARTER, W.G., EVANS, M.J.C., VENNARD, R.A., SRIBALA-SUNDARAM, S. and NIMMO, M.A., 2012. Determination of inflammatory and prominent proteomic changes in plasma and adipose tissue after high-intensity intermittent training in overweight and obese males Journal of Applied Physiology. 112(8), 1353-1360
• CARTER WG, 2012. Utilisation of chromatographic and electrophoretic separation techniques for the detection of protein post-translational modifications. Medicinal Chemistry. S11, 002
• CARTER WG, 2012. Detection and quantification of protein post-translational modifications using novel microchannel plate autoradiographic imagers. Medicinal Chemistry. S11, 001
• TARHONI, M.H., VIGNESWARA, V., SMITH, M., ANDERSON, S., WIGMORE, P., LEES, J.E., RAY, D.E. and CARTER, W.G., 2011. Detection, quantification, and microlocalisation of targets of pesticides using microchannel plate autoradiographic imagers Molecules. 16(10), 8535-8551
• CARTER, W.G., TARHONI, M.H. and RAY, D.E., 2010. Analytical approaches to investigate protein-pesticide adducts Journal of Chromatography B. 878(17-18), 1312-1319
• KHARBANDA, K.K., VIGNESWARA, V., MCVICKER, B.L., NEWLACZYL, A.U., BAILEY, K., TUMA, D., RAY, D.E. and CARTER, W.G., 2009. Proteomics reveal a concerted upregulation of methionine metabolic pathway enzymes, and downregulation of carbonic anhydrase-III, in betaine supplemented ethanol-fed rats Biochemical and Biophysical Research Communications. 381(4), 523-527
• TARHONI, MABRUKA H, LISTER, TIMOTHY, RAY, DAVID E and CARTER, WAYNE G, 2008. Albumin binding as a potential biomarker of exposure to moderately low levels of organophosphorus pesticides. Biomarkers. 13(4), 343-63
• CARTER, W.G. and ASWAD, D.W., 2008. Formation, localization, and repair of L-isoaspartyl sites in histones H2A and H2B in nucleosomes from rat liver and chicken erythrocytes Biochemistry. 47(40), 10757-10764
• JETHWA, P.H., WARNER, A., NILAWEERA, K.N., BRAMELD, J.M., KEYTE, J.W., CARTER, W.G., BOLTON, N., BRUGGRABER, M., MORGAN, P.J., BARRETT, P. and EBLING, F.J.P., 2007. VGF-derived peptide, TLQP-21, regulates food intake and body weight in Siberian hamsters Endocrinology. 148(8), 4044-4055
• CARTER, WAYNE G, TARHONI, MABRUKA, RATHBONE, ALEXANDRA J and RAY, DAVID E, 2007. Differential protein adduction by seven organophosphorus pesticides in both brain and thymus. Human and Experimental Toxicology. 26(4), 347-54
• SKAMARAUSKAS, J, CARTER, W, FOWLER, M, MADJD, A, LISTER, T, MAVROUDIS, G and RAY, D E, 2007. The selective neurotoxicity produced by 3-chloropropanediol in the rat is not a result of energy deprivation. Toxicology. 232(3), 268-76
• VIGNESWARA, V., LOWENSON, J.D., POWELL, C.D., THAKUR, M., BAILEY, K., CLARKE, S., RAY, D.E. and CARTER, W.G, 2006. Proteomic identification of novel substrates of a protein isoaspartyl methyltransferase repair enzyme. The Journal of biological chemistry. 281(43), 32619-32629
• YOUNG, A L, CARTER, W G, DOYLE, H A, MAMULA, M J and ASWAD, D W, 2001. Structural integrity of histone H2B in vivo requires the activity of protein L-isoaspartate O-methyltransferase, a putative protein repair enzyme. Journal of Biological Chemistry. 276(40), 37161-5
• FINCH, A, DAVIS, W, CARTER, W G and SAKLATVALA, J, 2001. Analysis of mitogen-activated protein kinase pathways used by interleukin 1 in tissues in vivo: activation of hepatic c-Jun N-terminal kinases 1 and 2, and mitogen-activated protein kinase kinases 4 and 7. Biochemical Journal. 353(Pt 2), 275-81
• CARTER, W G, SULLIVAN, A C, ASAMOAH, K A and SALE, G J, 1996. Purification and characterization of an insulin-stimulated insulin receptor serine kinase. Biochemistry. 35(45), 14340-51
• ASAMOAH, K A, ATKINSON, P G, CARTER, W G and SALE, G J, 1995. Studies on an insulin-stimulated insulin receptor serine kinase activity: separation of the kinase activity from the insulin receptor and its reconstitution back to the insulin receptor. Biochemical Journal. 308 ( Pt 3), 915-22
• CARTER, W G, ASAMOAH, K A and SALE, G J, 1995. Studies into the identity of the sites of insulin-stimulated insulin receptor serine phosphorylation. Characterization of synthetic peptide substrates for the insulin-stimulated insulin receptor serine kinase. Biochemistry. 34(29), 9488-99