One Virology

Applying next-generation phage display to identify viral epitopes

Next-generation phage display (NGPD) represents a step change in the phage display approach by incorporating highly advanced sequencing processes.

We’ve recently been applying our expertise in NGPD to a series of research projects, identifying short amino acid sequences of protein (epitopes) that are recognised by antibodies generated when animals are infected with specific viruses. The aim is to use these to determine whether the antibodies are a result of infection or vaccination (Differentiation of Infected from Vaccinated Animals) or to distinguish between antibodies resulting from infection with very closely-related viruses that traditional assays cannot tell apart.

Spotlight projects

Diagnostic tests to differentiate infected from vaccinated animals (DIVA)

In our first project (funded by Innovate UK and the BBSRC), the aim was to develop a field-based diagnostic test for detecting antibodies to SBV. This would give farmers a cost-effective tool for determining what proportion of their flock or herd already had antibodies that were likely to protect against the virus enabling them make an informed decision about whether to vaccinate.

Our second project, also funded by the BBSRC, explored how we could develop a diagnostic test that provides reassurance that antibodies detected in an animal are because they have been vaccinated and not because they have been infected.


One Virology

University of Nottingham