Stephen Harding

Professor of Applied Biochemistry, Faculty of Science

Personal Details
Media
Research
Publications

Contact

workNCMH Building
Sutton Bonington Campus
Sutton Bonington
Leicestershire
LE12 5RD
UK
work0115 951 6148
fax0115 951 6142
steve.harding@nottingham.ac.uk
http://www.nottingham.ac.uk/-sczsteve/research.htm

Biography

Born: Wallasey, Wirral 2^nd August 1955. Education: Wallasey Grammar and Marple Hall Grammar Schools; Pembroke College, Oxford (MA, 1980). Employment: Lister PostDoc, University of Bristol (81-82); Oppenheimer Postdoc, University of Cambridge (83-84); Supervisor (Queens College, Cambridge, 84); University of Nottingham (84-present). Visiting Professor: Institute für Organische Chemie und Makromolekulare Chemie, Friedrich Schiller Universität, Jena, Federal Republic of Germany (2009-2013). Awards: Royal Society of Chemistry Junior Medallist (1991); Oxford University DSc (2002); Ridder av 1. klasse, den Kongelige Norske Fortjenstorden, - Knight of the 1st class, the Royal Norwegian Order of Merit (2012).

Video of Steve getting DSc - November 2002; Video clip from Steve's Inaugural Lecture - December 2002; Knight's Cross of den Kongelige Norske Fortjenstorden (photo, M. McCartney); BBC NW coverage of the Award Ceremony - March 2012; BBC's Phillip Bilson plays Bach's Fantasia in G-dur at Steve & Anne's wedding, Thurnby, 2.2.80; Wikipedia Norway list of people from the Wirral Peninsula

Research Summary

Selected Publications

GARCÍA DE LA TORRE, J. and HARDING, S.E., 2013. Hydrodynamic modelling of protein conformation in solution: ELLIPS and HYDRO Biophysical Reviews. 5, 195-206
HEINZE, T., NIKOLAJSKI, M., DAUS, S., BESONG, T.M.D., MICHAELIS, N., BERLIN, P., MORRIS, G.A., ROWE, A.J. and HARDING, S.E., 2011. Protein-like oligomerisation of carbohydrates Angewandte Chemie International Edition. 50(37), 8602-8604
HARDING, S.E., SCHUCK, P., ABDELHAMMED, A.S., ADAMS, G., KÖK, M.S. and MORRIS, G.A., 2011. Extended Fujita approach to the molecular weight distribution of polysaccharides and other polymer systems Methods. 54(1), 136-144
BOWDEN, G.R., BALARESQUE, P., KING, T.E., HANSEN, Z., LEE, A.C., PERGL-WILSON, G., HURLEY, E., ROBERTS, S.J., WAITE, P., JESCH, J., JONES, A.L., THOMAS, M.G., HARDING, S.E. and JOBLING, M.A., 2008. Excavating past population structures by surname-based sampling: the genetic legacy of the Vikings in Northwest England Molecular Biology and Evolution. 25(2), 301-309

View all publications

Radio/TV:

BBC NW (March 14th 2012)

BBC Radio Merseyside (May 29th 2011)

BBC Radio4 (May 20th 2008)

BBC Radio 4 (October 18th 2004)

Newspapers:

The Guardian (December 3rd 2007)

Morgunbladid (June 6th 2008)

For full list (59 Radio/TV broadcasts and 111 Newspaper articles) see: http://www.nottingham.ac.uk/-sczsteve/research.htm

Current Research

Professor Harding has been director of the NCMH (National Centre for Macromolecular Hydrodynamics) for the last 25 years, a period which has seen hydrodynamic methods (analytical ultracentrifugation, light scattering, viscometry etc.) come from almost oblivion to flourish as tools for biomolecular characterisation. He has published over 360 research papers and 10 books in the field of biomolecular hydrodynamics and applied biochemistry, assisted by grants in which he has been PI totalling over £5M. In recognition for his discoveries he received a junior medal from the Royal Society of Chemistry in 1991 and a DSc from the University of Oxford in 2002. In 2010 he gave the annual Pfizer lecture at the University of Connecticut and in 2012 he was made a Ridder 1. klasse den Kongelige Norske Fortjenstorden - Knight of the 1st class of the Royal Norwegian Order of Merit - by King Harald V of Norway. He is listed on Wikipedia Norway's list of 10 most notable people from the Wirral peninsula. Although he has contributed extensively towards our understanding of the hydrodynamic properties of proteins his main area has been on polysaccharides and glycoconjugates, and in developing methods for understanding their size distributions, conformations and interactions. His current focus is on biopolymer stability in response to bioprocessing, of particular interest to the Biopharma industry where he now draws much of his support.

Development of hydrodynamic methodologies: 1. Molecular weight distributions of polymers using ultracentrifugation. 2. the study of conformation in mixed, aggregated and bioprocessed systems. 3. The non-ideality problem in solution biophysics, and its accurate measurement and prediction using COVOL. Glycobiology research: 1. Polysaccharide conformation and stability in solution; 2. Stability of glycoconjugate vaccines; 3. Polysaccharide interactions in health and disease; 4. Antibody structure and stability in solution; 5. Mucus glycoprotein structure & mucoadhesion. DNA Research: Y-chromosome DNA (with Professor Mark Jobling, University of Leicester). This began with a prestigious Watson-Crick DNA anniversary award from the BBSRC.

Support: The Royal Society, BBSRC, EPSRC, Wellcome Trust, Industrial (GSK Rixensart, Medimmune, Inovo/Glycomix, Kelloggs); Research papers (363): http://www.nottingham.ac.uk/ncmh/harding_publish.html; Books (14): Stephen Hardings Amazon.co.uk page and Amazon.com page; Links: Steve's series of 10 lectures from the Friedrich-Schiller Universität, Jena.

GARCÍA DE LA TORRE, J. and HARDING, S.E., 2013. Hydrodynamic modelling of protein conformation in solution: ELLIPS and HYDRO Biophysical Reviews. 5, 195-206
HARDING, S.E., ed., 2013. Section "Molecular Shape and Hydrodynamics" (In: Encyclopedia of Biophysics, General Editor: Roberts, G.C.K). Springer Verlag, Berlin.
HARDING, S.E., 2013. ELLIPS and COVOL. In: ROBERTS, G.C.K, ed., Encyclopedia of Biophysics, Springer Verlag, Berlin. 654-662
MORRIS, G.A and HARDING, S.E., 2013. Hydrodynamic modelling of carbohydrate polymers. In: ROBERTS, G.C.K., ed., Encylopedia of Biophysics, Springer Verlag. 1006-1014
HARDING, S.E., 2013. Intrinsic viscosity. In: ROBERTS, G.C.K., ed., Encylopedia of Biophysics, Springer Verlag, Berlin. 1123-1129
HARDING, S.E., 2013. Polysaccharides: biophysical properties. In: ROBERTS, G.C.K., ed., Encyclopedia of Biophysics, Springer Verlag, Berlin. 1907-1912
GILLIS RB, ADAMS GG, WOLF B, BERRY M, BESONG TMD, CORFIELD A, KÖK SM, SIDEBOTTOM R, LAFOND D, ROWE AJ and HARDING SE, 2013. Molecular weight distribution analysis by ultracentrifugation: adaptation of a new approach for mucins. Carbohydrate polymers. 93(1), 178-83
FEIGENBUTZ M, JONES R, BESONG TMD, HARDING SE and MITCHELL P, 2013. Assembly of the Yeast Exoribonuclease Rrp6 with its Associated Cofactor Rrp47 Occurs in the Nucleus and is Critical for the Controlled Expression of Rrp47. Journal of Biological Chemistry. (In Press.)
KÖK, M.S., GILLIS, R., ANG, S., LAFOND, D., TATHAM, A.S., ADAMS, G. and HARDING, S., 2012. Can dietary fibre help provide safer food products for sufferers of gluten intolerance? A well-established biophysical probe may help towards providing an answer BMC Biophysics. 5, 10
HARDING SE, 2012. Meeting report: the stability and degradation of complex carbohydrate structures: mechanisms and measurement. Biotechnology & Genetic Engineering Reviews. 28, 177-182
ADAMS GG, KÖK MS, IMRAN S, HARDING SE, ILYAS M and TATHAM AS, 2012. The interaction of dietary fibres with disulphide bonds (S-S) and a potential strategy to reduce the toxicity of the gluten proteins in coeliac disease. Biotechnology & Genetic Engineering Reviews. 28, 115-130
IMRAN S, GILLIS RB, KOK MS, HARDING SE and ADAMS GG, 2012. Application and use of inulin as a tool for therapeutic drug delivery. Biotechnology & Genetic Engineering Reviews. 28, 33-45
BESONG TMD, HARDING SE and WINZOR DJ, 2012. The effective time of centrifugation for the analysis of boundary spreading in sedimentation velocity experiments. Analytical Biochemistry. 421, 755-758
HARDING SE, ALMUTAIRI F, ADAMS GG, LAWSON CJ, WOOD S and GAHLER RJ, 2012. Dilute solution viscometry studies on a therapeutic mixture of non-digestible carbohydrates International Journal of Biotechnology for Wellness Industries. 1, 107-114
ADAMS GG, IMRAN S, WANG S, MOHAMMAD A, KOK MS, GRAY DA, CHANNELL and HARDING SE, 2012. Extraction, isolation and characterisation of oil bodies from pumpkin seeds for therapeutic use. Food Chemistry. 134, 1919-1925
ABDELHAMEED AS, MORRIS GA, ADAMS GG, ROWE AJ, LALOUX O, CERNY L, BONNIER B, DUVIVIER P, CONRATH K, LENFANT C and HARDING SE, 2012. An asymmetric and slightly dimerized structure for the tetanus toxoid protein used in glycoconjugate vaccines. Carbohydrate Polymers. 90, 1831-1835
HARDING, SE, ABDELHAMEED, AS, MORRIS, GA, ADAMS, GG, LALOUX, O, CERNY, L, BONNIER, B, DUVIVIER, P, CONRATH, K and LENFANT, C, 2012. Solution properties of capsular polysaccharides from Streptococcus pneumoniae Carbohydrate Polymers. 90, 237-242
HARDING, S.E., ed., 2012. Stability of Complex Carbohydrate Structures: Biofuels, Foods, Vaccines and Shipwrecks Royal Society of Chemistry, Cambridge U.K.
HARDING, S.E., 2012. Preface. In: HARDING, S.E., ed., Stability of Complex Carbohydrate Structures: Biofuels, Foods, Vaccines and Shipwrecks Royal Society of Chemistry, Cambridge, U.K. P005-P007
MORRIS, G.A., ADAMS, G.G., HARDING, S.E., CASTILE, J.D. and SMITH, A., 2012. Stability of Pectin-Based Drug Delivery Systems. In: HARDING, S.E., ed., Stability of Complex Carbohydrate Structures: Biofuels, Foods, Vaccines and Shipwrecks Royal Society of Chemistry, Cambridge U.K. 99-109
HARDING, S.E., 2012. Viscometry, Analytical Ultracentrifugation and Light Scattering Probes for Carbohydrate Stability. In: HARDING, S.E., ed., Stability of Complex Carbohydrate Structures: Biofuels, Foods, Vaccines and Shipwrecks Royal Society of Chemistry, Cambridge U.K. 80-98
HARDING, S.E, SMITH, I.H, LAWSON, C.J and GAHLER, R.J. AND WOOD, S., 2011. Studies on macromolecular interactions in ternary mixtures of konjac glucomannan, xanthan gum and sodium alginate Carbohydrate Polymers. 83, 329-338
ADAMS, G.G., IMRAN, S., MOHAMMAD, A., KOK, S., GRAY. D.A., CHANNELL, G.A., MORRIS, G.A. and HARDING, S.E., 2011. The hypoglycaemic effect of pumpkins as anti-diabetic and functional medicines Food Research International. 44, 862-867
HEINZE, T., NIKOLAJSKI, M., DAUS, S., BESONG, T.M.D., MICHAELIS, N., BERLIN, P., MORRIS, G.A., ROWE, A.J. and HARDING, S.E., 2011. Proteinähnliche Oligomerisierung von Kohlenhydraten Angewandte Chemie. 87, 8761-8763
HEINZE, T., NIKOLAJSKI, M., DAUS, S., BESONG, T.M.D., MICHAELIS, N., BERLIN, P., MORRIS, G.A., ROWE, A.J. and HARDING, S.E., 2011. Protein-like oligomerisation of carbohydrates Angewandte Chemie International Edition. 50(37), 8602-8604
HARDING, S.E., ABDELHAMEED, A.S. and MORRIS, G.A., 2011. On the hydrodynamic analysis of conformation in mixed biopolymer systems Polymer International. 60, 2-8
MORRIS, G.A., CASTILE, J., ADAMS, G.G. and HARDING, S.E., 2011. The effect of prolonged storage at different temperatures on the particle size distribution of tripolyphosphate (TPP) - chitosan nanoparticles Carbohydrate Polymers. 84, 1430-1434
HARDING, S.E., SCHUCK, P., ABDELHAMMED, A.S., ADAMS, G., KÖK, M.S. and MORRIS, G.A., 2011. Extended Fujita approach to the molecular weight distribution of polysaccharides and other polymer systems Methods. 54(1), 136-144
HARDING, S.E., 2011. Paley Johnson, 1917-2011 The Biochemist. 33, 66-67
HARDING, S.E., 2011. Paley Johnson 12 February 1917 - 11 February 2011: Fellow of Trinity 1962-2011 Trinity College Cambridge Annual Record. 139-145
PATEL TR, BESONG TMD, PATEL N, MEIER M, HARDING SE, WINZOR DJ and STETEFELD J, 2011. Evidence for self-association of a miniaturized version of agrin from hydrodynamic and small-angle x-ray scattering measurements. Journal of Physical Chemistry, B. 115(38), 11286-93
ADAMS, G.G., BUTTERY, L., STOLNIK, S., HARDING, S.E. and WANG, N., 2011. Assessment of Biological Properties of Mouse Embryonic Stem Cells Characteristics Prior To Differentiation Journal of Biomaterials and Nanobiotechnology. 378-389
HARDING, S.E., ABDELHAMEED, A.S. and MORRIS, G.A., 2010. Molecular weight distribution evaluation of polysaccharides and glycoconjugates using analytical ultracentrifugation Macromolecular Bioscience. 10(7), 714-720
MORRIS, G.A., KÖK, M.S. and HARDING, S.E. AND ADAMS, G.G., 2010. Polysaccharide drug delivery systems based on pectin and chitosan Biotechnology and Genetic Engineering Reviews. 27, 257-284
HARDING, S.E. and ROWE, A.J., 2010. Insight into protein-protein interactions from analytical ultracentrifugation. Biochemical Society Transactions. 38, 901-907
HARDING, S.E. AND WINZOR, D.J., 2010. James Michael Creeth (1924-2010) The Biochemist. 32, 44-45
PATEL, T.R., MORRIS, G.A., ZWOLANEK, D., KEENE, D.R., LI, J., HARDING, S.E., KOCH, M. and STETEFELD, J., 2010. Nano-structure of the laminin γ-1 short arm reveals an extended and curved multidomain assembly Matrix Biology. 29, 565-572
CARDINALI, B, PROFUMO, A, APRILE, A, BYRON, O, MORRIS, G.A, HARDING, S.E and STAFFORD, W.F. AND ROCCO, M., 2010. Hydrodynamic and mass spectrometry analysis of nearly-intact human fibrinogen, chicken fibrinogen, and of a substantially monodisperse human fibrinogen fragment X Archives of Biochemistry and Biophysics. 493, 157-168
HARDING, S.E., 2010. Some observations on the effects of bioprocessing on biopolymer stability. Journal of Drug Targeting. 18, 732-740
HARDING, S.E. and WINZOR, D.J., 2010. James Michael Creeth, 1924-2010. Macromolecular Bioscience. 10, 696-699
ABDELHAMEED, A.S, ANG, S, MORRIS, G.A, SMITH, I, LAWSON, C, GAHLER, R and WOOD, S. AND HARDING, S.E., 2010. An analytical ultracentrifuge study on ternary mixtures of konjac glucomannan supplemented with sodium alginate and xanthan gum Carbohydrate Polymers. 81, 145-148
HARDING, S.E., 2010. Dr. Michael Creeth. Scientist who helped pave the way for Watson and Crick The Independent, 31st March. 39
HARDING, S.E., JOBLING, M. and KING, T.E., 2010. Viking DNA: The Wirral and West Lancashire Project Nottingham University Press.
ADAMS, G.G, BUTTERY, L, STOLNIK, S, MORRIS, G.A and HARDING, S.E. AND WANG, N., 2010. Stem cells: the therapeutic role in the treatment of diabetes mellitus Biotechnology and Genetic Engineering Reviews. 27, 285-304
MORRIS, G.A, RALET, R.C, BONNIN, E, THIBAULT, J.F and HARDING, S.E., 2010. Physical characterisation of the rhamnogalcturonan and homogalacturonan fractions of sugar beet (Beta vulgaris) pectin Carbohydrate Polymers. 82, 1161-1167
MORRIS, G.A., CASTILE, J., SMITH, A., ADAMS, G.G. and HARDING, S.E., 2010. The effect of different storage temperatures on the physical properties of pectin solutions and gels Polymer Degradation and Stability. 95(12), 2670-2673
ANG, S., KOGULANATHAN, J., MORRIS, G.A., KÖK, M.S., SHEWRY, P.R., TATHAM, A.S., ADAMS, G.G., ROWE, A.J. and HARDING, S.E., 2010. Structure and heterogeneity of gliadin: a hydrodynamic evaluation European Biophysics Journal. 39(2), 255–261
AMORÓS D, ORTEGA A, HARDING SE and GARCÍA DE LA TORRE J, 2010. Multi-scale calculation and Global-fit analysis of hydrodynamic Properties Of Biological Macromolecules: Determination of the overall conformation of antibody IgG molecules. European Biophysics Journal. 39, 361-370
MORRIS, G.A., CASTILE, J., SMITH, A. and ADAMS, G.G, AND HARDING, S.E., 2009. Macromolecular conformation of chitosan in dilute solution: a new global hydrodynamic approach Carbohydrate Polymers. 76, 616-621
YADAV, N, MORRIS, G, HARDING, S.E and ANG, S. AND ADAMS, G. G., 2009. Various non-injectable delivery systems for the treatment of Diabetes mellitus Endocrine, Metabolic and Immune Disorders-Drug Targets. 9, 1-13
MORRIS, G.A., CASTILE, H., SMITH, A. and ADAMS, G.G. AND HARDING, S.E., 2009. The kinetics of chitosan depolymerisation at different temperatures Polymer Degradation and Stability. 94,, 1334-1348.
KÖK, M. S, ABDELHAMEED, A. S, ANG, S, MORRIS, G. A and AND HARDING, S. E., 2009. A novel global hydrodynamic analysis of the molecular flexibility of the dietary fibre polysaccharide konjac glucomannan Food Hydrocolloids. 23, 1910-1917
MORRIS, G.A. and HARDING, S.E., 2009. Polysaccharides, Microbial. In: SCHAECHTER, M., ed., Encyclopedia of Microbiology 3rd ed. Amsterdam: Elsevier. 482-494
POHL, M., MORRIS, G.A., HARDING, S.E. and HEINZE, T., 2009. Studies on the molecular flexibility of novel dendronized carboxymethyl cellulose derivatives European Polymer Journal. 45, 1098-1110
WANDREY, C and BARTKOWIAK, A. AND HARDING, S.E., 2009. Materials for encapsulation. In: ZUIDAM, N.J. AND NEDOVIC, V., ed., Encapsulation Technologies for Active Food Ingredients and Food Processing Springer, New York. 31-100
DESSE, M, ANG, S, MORRIS, G.A, ABU-HARDAN, M, WOLF, B,, HILL, S.E, HARDING, S.E, BUDTOVA, T and MITCHELL, J.R., 2009. Analysis of the continuous phase of the modified waxy maize suspension Carbohydrate Polymers. 77, 320-325
HARDING, S.E., 2009. Combined hydrodynamic approach to size, heterogeneity, conformation and flexbility of bio-based macromolecules In: European Polymer Congress (EPF09) IL1-9. 107
MORRIS, G. A., ANG, S., HILL, S. E., LEWIS, S., SHAFER, B., NOBBMANN, U. and HARDING, S. E., 2008. Molar mass and solution conformation of branched α(1 → 4), α(1 → 6) Glucans. Part I: Glycogens in water Carbohydrate Polymers. 71, 101-108
KOSCHELLA, A., INNGJERDINGEN, K., PAULSEN, B.S., MORRIS, G.A., HARDING, S.E. and HEINZE, T., 2008. Unconventional methyl galactan synthesized via the thexyldimethylsilyl intermediate: preparation, characterization, and properties. Macromolecular Bioscience. 8, 96-105
PATEL,T.R., MORRIS, G.A., GARCIA DE LA TORRE, J., ORTEGA, A., MISCHNICK, P. and HARDING, S.E., 2008. Molecular flexibility of methylcelluloses of differing degree of substitution by combined sedimentation and viscosity analysis. Macromolecular Bioscience. 8, 1108-1115
MORRIS, G.A, GARCÍA DE LA TORRE, J, ORTEGA, A, CASTILE, J, SMITH, A and AND HARDING, S.E., 2008. Molecular flexibility of citrus pectins by combined sedimentation and viscosity analysis Food Hydrocolloids. 22, 1435-1442
BOWDEN, G.R., BALARESQUE, P., KING, T.E., HANSEN, Z., LEE, A.C., PERGL-WILSON, G., HURLEY, E., ROBERTS, S.J., WAITE, P., JESCH, J., JONES, A.L., THOMAS, M.G., HARDING, S.E. and JOBLING, M.A., 2008. Excavating past population structures by surname-based sampling: the genetic legacy of the Vikings in Northwest England Molecular Biology and Evolution. 25(2), 301-309
INNGJERDINGEN, M., INNGJERDINGEN, K.T., PATEL, T.R., ALLEN, S., CHEN, X., ROLSTAD, B., MORRIS, G.A., HARDING, S.E., MICHAELSEN, T.E., DIALLO, D. and PAULSEN, B., 2008. Pectic polysaccharides from Biophytum petersianum Klotzsch, and their activation of macrophages and dendritic cells. Glycobiology. 18, 1074-184
MORRIS, G. A., PATEL, T. R., PICOUT, D. R., ROSS-MURPHY, S. B., ORTEGA, A., GARCIA DE LA TORRE, J. and HARDING S. E., 2008. Global hydrodynamic analysis of the molecular flexibility of galactomannans Carbohydrate Polymers. 72, 356-360
LU, Y., HARDING, S.E., TURNER, A., SMITH, B., ATHWAL, D.S., GROSSMANN, J.G., DAVIS, K.G. and ROWE, A.J., 2008. Effect of PEGylation on the solution conformation of antibody fragments Journal of Pharmaceutical Science. 97(6), 2062-2079
GRIFFITHS, D, HARDING, S.E and JOBLING, M.A., 2008. Looking for Vikings in north-west England British Archaeology. 103, 18-25
ISRAILIDES, C, KLETSAS, D, ARAPOGLOU, D, PHILIPPOUSSIS, A, PRATSINIS, H, EBRINGEROVA, A, HRIBALOVA, V and HARDING, SE, 2008. In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes. Phytomedicine. 15, 512-519
PATEL, T.R., MORRIS, G.A., EBRINGEROVÁ, A., VODENICAROVÁ, M., VELEBNY, V., ORTEGA, A., GARCIA DE LA TORRE, J.G. and HARDING, S.E., 2008. Global conformation analysis of irradiated xyloglucans. Carbohydrate Polymers. 74, 845-851
LU, Y, HARDING, S.E, ROWE, A.J, DAVIS, K.G, FISH, B, VARLEY, P, GEE, C and MULOT, S., 2008. The effect of a point mutation on the stability of IgG4 as monitored by analytical ultracentrifugation Journal of Pharmaceutical Science. 97, 948-957
PATEL, T.R, HARDING, S.E, EBRINGEROVA, A., DESZCZYNSKI, M., HROMADKOVA, Z., TOGOLA, A., PAULSEN, B.S., MORRIS, G.A and ROWE, A.J., 2007. Weak self-association in a carbohydrate system Biophysical Journal. 93(3), 741-749
INNGJERDINGEN, K.T., PATEL, T., CHEN, X., KENNE, L., ALLEN, S., MORRIS, G.A., HARDING, S.E., MATSUMOTO, T., DIALLO, D., YAMADA, H., MICHAELSEN, T.E., INNGJERDINGEN, M. and PAULSEN, B.S., 2007. Immunological and structural properties of a pectic polymer from Glinus oppositifolius. Glycobiology. 17, 1299-1310
LU, Y, HARDING, S.E, MICHAELSEN, T.E, LONGMAN, E, DAVIS, K.G, ORTEGA, A and GROSSMANN, J.G. SANDLIE, I. AND GARCIA DE LA TORRE, J, 2007. Solution conformation of wild type and mutant IgG3 and IgG4 immunoglobulins using Crystallohydrodynamics: possible implications for complement activation Biophysical Journal. 93, 3733-3744
NOBBMANN, U, CONNAH, M, FISH, B, VARLEY, P, GEE, C, MULOT, S, CHEN, J, ZHOU, L, LU, Y, SHEN, F, YI, J and HARDING, S.E., 2007. Dynamic light scattering as a relative tool for assessing the molecular integrity and stability of monoclonal antibodies. Biotechnology and Genetic Engineering Reviews. 24, 117-128
HARDING, S.E., 2007. The Wirral carrs and holms Journal of the English Place-Name Society. 39, 45-57
WINZOR DJ, DESZCZYNSKI M, HARDING SE and WILLS PR, 2007. Nonequivalence of second virial coefficients from sedimentation equilibrium and static light scattering studies of protein solutions. Biophysical chemistry. 128(1), 46-55
LU, Y., LONGMAN, E., DAVIS, K.G, ORTEGA, A., GROSSMANN, J.G., MICHAELSEN, T.E, DE LA TORRE, J.G. and HARDING, S.E., 2006. Crystallohydrodynamics of protein assemblies: combining sedimentation, viscometry, and x-ray scattering Biophysical Journal. 91(5), 1688-1697
DESZCZYNSKI, M., HARDING, S.E and WINZOR, D.J., 2006. Negative second virial coefficients as predictors of protein crystal growth: evidence from sedimentation equilibrium studies that refutes the designation of those light scattering parameters as osmotic virial coefficients. Biophysical Chemistry. 120, 106-113
HARDING, S.E., 2006. Trends in mucoadhesive analysis Trends in Food Science and Technology. 17, 255-262
BEDFORD, C, CASS, A and FRANCOIS, I. AND HARDING, S.E., 2006. Glycomics: from glycobiology to diagnostics and therapeutics Drug News Perspect. 19, 163-172
HUGHES, R.K, BELFIELD, E.J, MUTHUSAMAY, M, KHAN, A, ROWE, A, HARDING, S.E, FAIRHURST, S.A, BORNEMANN, S, ASHTON, R, THORNELEY, R.N.F and CASEY, R., 2006. Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer-micelle association. Biochemical Journal. 395, 641-652
HARDING, S.E., 2006. Ingimund's Saga: Norwegian Wirral (2nd edition, hardback) Countyvise Limited.
PATEL, T. R, PICOUT, D. R and HARDING, S. E. AND ROSS-MURPHY, S. B., 2006. Pressure cell assisted solution characterization of galactomannans. 3. Application of analytical ultracentrifugation techniques. Biomacromolecules. 7, 3513-3520
LONGMAN, E., HARDING, S.E. and MARHEINEKE, N., 2006. Identifying differences in solution conformation of two chimeric IgG3 antibodies through triple detection SEC LCGC North America. 24, 64-72
KHAN, A, HUGHES, R.K, BELFIELD, E.J, CASEY, R. ROWE, A.J and HARDING, S.E., 2006. Oligomerization of hydroperoxide lyase, a novel P450 enzyme in plants Progress Colloid Polymer Sci.. 131, 116-120
TESTER, RICHARD F, PATEL, TRUSHAR and HARDING, STEPHEN E, 2006. Damaged starch characterisation by ultracentrifugation. Carbohydrate Research. 341, 130-137
HARDING, S.E., 2005. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides Carbohydrate Research. VOL 340(NUMBER 5), 811-826
HARDING, S.E., CÖLFEN, H. and AZIZ, Z., 2005. The ELLIPS suite of whole-body protein conformation algorithms for Microsoft Windows. In: SCOTT, D.J., HARDING, S.E. and ROWE, A.J., eds., Analytical Ultracentrifugation. Techniques and Methods Cambridge, UK: Royal Society of Chemistry. 460-483
LONGMAN, E., HARDING, S.E. and MARHEINEKE, N., 2005. Identifying differences in solution conformation of two chimeric IgG3 antibodies through triple detection SEC LCGC (Liquid Chromatography, Gas Chromatography). 18, 662-668
CHEBOTAREVA, N. A., KURGANOV, B. I., HARDING, S. E. and WINZOR, D. J., 2005. Effect of osmolytes on the interaction of flavin adenine dinucleotide with muscle glycogen phosphorylase b Biophysical Chemistry. VOL 113, 61-66
NEGARD, C.S, MATSUMOTO, T. INNGJERDINGEN, M, INNGJERDINGEN, K., HOKPUTSA, S, HARDING, S.E. DIALLO, D, KIYOHARA, H, SMESTAD PAULSEN, B and YAMADA, H., 2005. Structural and immunological studies of a pectin and a pectic arabinogalactan from Vernonia kotschyana Sch. Bip. ex Walp. (Asteraceae). Carbohydrate Research. 340, 115-130
HARDING, S.E., 2005. Analysis of polysaccharides by ultracentrifugation: size, conformation and interactions in solution Advances in Polymer Science (Special edition "Polysaccharides I: Structure, Characterisation and Use" edited by T. Heinze). 186, 211-254
HARDING, S.E., 2005. Analysis of polysaccharide size, shape and interactions. In: SCOTT, D.J., HARDING, S.E. and ROWE, A.J., eds., Analytical ultracentrifugation. Techniques and Methods. Cambridge, UK: Royal Society of Chemistry. 231-252
SCOTT, D.J, HARDING, S.E and ROWE, A.J., eds., 2005. Analytical ultracentrifugation. Techniques and Methods Royal Society of Chemistry, Cambridge.
INNGJERDINGEN, KARI TVETE, DEBES, SYLVI C, INNGJERDINGEN, MARIT, HOKPUTSA, SANYA, HARDING, STEPHEN E, ROLSTAD, BENT, MICHAELSEN, TERJE E, DIALLO, DRISSA and PAULSEN, BERIT SMESTAD, 2005. Bioactive pectic polysaccharides from Glinus oppositifolius (L.) Aug. DC., a Malian medicinal plant, isolation and partial characterization. Journal of Ethnopharmacology. 101, 204-214
CAVILL, P., HARDING, S.E. and JESCH, J., 2004. Revisiting Dingesmere Journal of the English Place-Name Society. 36, 25-38
PAULETA, S. R., COOPER, A., NUTLEY, M., ERRINGTON, N., HARDING, S., GUERLESQUIN, F., GOODHEW, C. F., MOURA, I., MOURA, J. J. G. and PETTIGREW, G. W., 2004. A copper protein and a cytochrome bind at the same site on bacterial cytochrome c peroxidase Biochemistry. 43, 14566-14576
FOSSATI, G., CREMONESI, P., IZZO, G., RIZZI, E., SANDRONE, G., HARDING, S., ERRINGTON, N., WALTERS, C., HENDERSON, B. and ROBERTS, M. M., 2004. The Mycobacterium tuberculosis chaperonin 10 monomer exhibits structural plasticity Biopolymers. 75, 148-162
HOKPUTSA, S., HARDING, S.E., INNGJERDINGEN, S., JUMEL, K., MICHAELSEN, T.E., HEINZE, T., KOSCHELLA, T. and PAULSEN, B.S., 2004. Bioactive polysaccharides from the stems of the Thai medicinal plant Acanthus ebracteatus: their chamical and physical features Carbohydrate Research. 339, 753-762
WINZOR, D. J., JONES, S. and HARDING, S. E., 2004. Determination of protein charge by capillary zone electrophoresis Analytical Biochemistry. VOL 333, 225-229
WINZOR, D. J., CARRINGTON, L. E. and HARDING, S. E., 2004. Limitations of the ultracentrifugal approach for measuring the effective net charge of a macroion Analytical Biochemistry. VOL 333, 114-118
SEIFERT, A, RAWEL, H.M and HARDING, S.E. AND KROLL, J., 2004. Characterization of bovine serum albumin/chlorogenic acid solution mixtures by analytical ultracentrifugation Progress in Colloid and Polymer Science. 127, 83-88
FLINT, J., NURIZZO, D., HARDING, S. E., LONGMAN, E., DAVIES, G. J., GILBERT, H. J. and BOLAM, D. N., 2004. Ligand-mediated dimerization of a carbohydrate-binding module reveals a novel mechanism for protein-carbohydrate recognition Journal of Molecular Biology. VOL 337, 417-426
HARDING, S. E., LONGMAN, E., CARRASCO, B., ORTEGA, A. and DE LA TORRE, J. G., 2004. Studying antibody conformations by ultracentrifugation and hydrodynamic Modeling Methods in Molecular Biology. VOL 248, 93-116
HARDING, S. E., LONGMAN, E., ORTEGA, A., KREUSEL, K., TENDLER, S. B., KING, K. and GARCIA DE LA TORRE, J., 2004. Use of the sedimentation coefficient for modelling antibodies. Refinements to the Crystallohydrodynamics Approach Progress in Colloid and Polymer Science. 127, 113-118
HOKPUTSA, S, GERDDIT, W, PONGSAMART, S, INNGJERDINGEN, K, HEINZE, T, KOSCHELLA, A, HARDING, S.E and PAULSEN, B.S., 2004. Water-soluble polysaccharides with phamaceutical importance from Durian rinds (Durio zibethinus Murr.): isolation, fractionation, characterisation and bioactivity Carbohydrate Polymers. 56, 471-481
WINZOR, D. J., CARRINGTON, L. E., DESZCZYNSKI, M. and HARDING, S. E., 2004. Extent of charge screening in aqueous polysaccharide solutions Biomacromolecules. VOL 5, 2456-2460
LEE, J. K., MOVAHEDI, S., HARDING, S. E. and WAITES, W. M., 2003. The effect of acid shock on sporulating Bacillus subtilis cells Journal of Applied Microbiology. VOL 94, 184-190
FEE, M., ERRINGTON, N., JUMEL, K., ILLUM, L., SMITH, A. and HARDING, S. E., 2003. Correlation of SEC/MALLS with ultracentrifuge and viscometric data for chitosans European Biophysics Journal. VOL 32, 457-464
LONGMAN, E., KREUSEL, K., TENDLER, S.J.B., FIEBRIG, I., KING, K., ADAIR, J., O'SHEA, P., ORTEGA, A., GARCIA DE LA TORRE, J. and HARDING, S.E., 2003. Estimating domain orientation of two human antibody IgG4 chimeras by crystallohydrodynamics. European Biophysics Journal. 32, 503-10
BORGES-WALMSLEY, M. I., BEAUCHAMP, J., KELLY, S. M., JUMEL, K., CANDLISH, D., HARDING, S. E., PRICE, N. C. and WALMSLEY, A. R., 2003. Identification of oligomerization and drug-binding domains of the membrane fusion protein EmrA Journal of Biological Chemistry. VOL 278, 12903-12912
MAJZOOBI, M., ROWE, A. J., CONNOCK, M., HILL, S. E. and HARDING, S. E., 2003. Partial fractionation of wheat starch amylose and amylopectin using zonal ultracentrifugation Carbohydrate Polymers. VOL 52, 269-274
HOKPUTSA, A., JUMEL, K., ALEXANDER, K. and HARDING, S.E., 2003. A comparison of molecular mass determination of hyaluronic acid using SEC/MALLS and sedimentation equilibrium European Biophysics Journal. 32, 450-456
HOKPUTSA, S., JUMEL, K., ALEXANDER, C. and HARDING, S. E., 2003. Hydrodynamic characterisation of chemically degraded hyaluronic acid Carbohydrate Polymers. VOL 52, 111-117
HARDING, S.E. AND O'SHEA, P., 2003. Biochemical interactions in 2D and 3D. Introductory remarks Biochemical Society Transactions. 31, 971-972
HARDING, S.E and O'SHEA, P., eds., 2003. Biochemical Society Transactions, Volume 31, pages 971-1051: Intermolecular Associations in 2D and 3D The Biochemical Society.
PETTIGREW, GRAHAM W, GOODHEW, CELIA F, COOPER, ALAN, NUTLEY, MARGARET, JUMEL, KORNELIA and HARDING, STEPHEN E, 2003. The electron transfer complexes of cytochrome c peroxidase from Paracoccus denitrificans. Biochemistry. 42, 2046-2055
HOKPUTSA, S., HU, C., PAULSEN, B. S. and HARDING, S. E., 2003. A physico-chemical comparative study on extracellular carbohydrate polymers from five desert algae Carbohydrate Polymers. VOL 54, 27-32
PETTIGREW, GRAHAM W, PAULETA, SOFIA R, GOODHEW, CELIA F, COOPER, ALAN, NUTLEY, MARGARET, JUMEL, KORNELIA, HARDING, STEPHEN E, COSTA, CRISTINA, KRIPPAHL, LUDWIG, MOURA, ISABEL and MOURA, JOSE, 2003. Electron transfer complexes of cytochrome c peroxidase from Paracoccus denitrificans containing more than one cytochrome. Biochemistry. 42, 11968-11981
PICOUT, DAVID R, ROSS-MURPHY, SIMON B, ERRINGTON, NEIL and HARDING, STEPHEN E, 2003. Pressure cell assisted solubilization of xyloglucans: tamarind seed polysaccharide and detarium gum. Biomacromolecules. 4, 799-807
HARDING, S. E., 2003. Mucoadhesive interactions Biochemical Society Transactions. VOL 31, 1036-1041
PETTIGREW, G.W., GOODHEW, C.F., COOPER, A., NUTLEY, M., JUMEL, K. and HARDING, S.E., 2003. The Electron Transfer Complexes Of Cytochrome C Peroxidase From Paracoccus Denitrificans Biochemistry. 42(7), 2046-2055
TIMOTEO, CG, TAVARES, P, GOODHEW, CF, DUARTE, LC, JUMEL, K, GIRIO, FMF, HARDING, S, PETTIGREW, GW and MOURA, I, 2003. Ca2+ And The Bacterial Peroxidases: The Cytochrome C Peroxidase From Pseudomonas Stutzeri Journal of Biological Inorganic Chemistry. 8(1-2), 29-37
WALTERS, C., ERRINGTON, N., ROWE, A.J. and HARDING, S.E., 2002. Hydrolysable ATP is a requirement for the correct interaction of molecular chaperonins cpn60 and cpn10 Biochemical Journal. 364(3), 849-855
JUMEL, K., HARDING, S. E., SOBOL, E., OMEL'CHENKO, A., SVIRIDOV, A. and JONES, N., 2002. Aspects of the structural integrity of chondroitin sulphate after laser irradiation Carbohydrate Polymers. VOL 48, 241-245
HARDING, S.E., TOMBS, M.P. and HARDING, S.E., 2002. The analytical ultracentrifuge as a probe for interface transport phenomena Biotechnology & Genetic Engineering Reviews. 55-69
PICOUT, DAVID R, ROSS-MURPHY, SIMON B, JUMEL, KORNELIA and HARDING, STEPHEN E, 2002. Pressure cell assisted solution characterization of polysaccharides. 2. Locust bean gum and tara gum. Biomacromolecules. 3, 761-767
HARDING, S.E., 2002. Viking Mersey Countyvise Limited, Birkenhead.
MORRIS, G.A., HROMADKOVA, Z., EBRINGEROVA, A., MALOVIKOVA, A., ALFOLDI, A. and HARDING, S.E., 2002. Modification of pectin with UV-absorbing substituents and its effect on the structural and hydrodynamic properties of the water-soluble derivatives Carbohydrate Polymers. 48, 351-359
CHEBOTAREVA, N. A., ANDREEVA, I. E., MAKEEVA, V. F., KURGANOV, B. I., LIVANOVA, N. B. and HARDING, S. E., 2002. Self-association of phosphorylase kinase from rabbit skeletal muscle in the presence of natural osmolyte, trimethylamine N-oxide Progress in Colloid and Polymer Science. VOL 119, 70-76
MORRIS, G. A., FOSTER, T. J. and HARDING, S. E., 2002. A hydrodynamic study of the depolymerisation of a high methoxy pectin at elevated temperatures Carbohydrate Polymers. VOL 48, 361-367
CARRASCO, B., GARCIA DE LA TORRE, J., DAVIS, K.G., JONES, S., ATHWAL, D., WALTERS, C., BURTON, D.R. and HARDING, S.E., 2001. Crystallohydrodynamics for solving the hydration problem for multi-domain proteins: open physiological conformations for human IgG Biophysical Chemistry. 93(2-3), 181-196
HUGHES, R. K., DESFORGES, N., SELWOOD, C., SMITH, R., SPEIRS, C. I., SINNAEVE, G., WISEMAN, J., JUMEL, K. and HARDING, S. E., 2001. Genes affecting starch biosynthesis exert pleiotropic effects on the protein content and composition of pea seeds Journal of The Science of Food and Agriculture. VOL 81, 877-882
LI, P., HARDING, S.E., LIU, Z. and HARDING, S.E., 2001. Cyanobacterial exopolysaccharides: their nature and potential biotechnological applications Biotechnology & Genetic Engineering Reviews. 18, 375-404
HARDING, S.E. and WINZOR, D.J., 2001. Sedimentation velocity analytical ultracentrifugation. In: HARDING, S.E. and CHOWDHRY, B.Z., eds., Protein-Ligand Interactions: Hydrodynamics and Calorimetry Oxford University Press. 75-103
MORRIS, G. A., LI, P., PUAUD, M., LIU, Z., MITCHELL, J. R. and HARDING, S. E., 2001. Hydrodynamic characterisation of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02: a comparison with xanthan Carbohydrate Polymers. VOL 44, 261-268
PAVLOV, G. M., ERRINGTON, N., HARDING, S. E., KORNEEVA, E. V. and ROY, R., 2001. Molecular and Structural Characteristics of Lactodendrimers Based on Poly(amidoamine) Polymer Science Series A. VOL 43, 118-123
PAVLOV, G. M., ERRINGTON, N., HARDING, S. E., KORNEEVA, E. V. and ROY, R., 2001. Dilute solution properties of lactosylated polyamidoamine dendrimers and their structural characteristics Polymer. VOL 42, 3671-3678
WINZOR, D.J. and HARDING, S.E., 2001. Sedimentation equilibrium in the analytical ultracentrifuge. In: HARDING, S.E. and CHOWDHRY, B.Z., eds., Protein-Ligand Interactions: Hydrodynamics and Calorimetry Oxford University Press. 105-135
CHEBOTAREVA, N. A., HARDING, S. E. and WINZOR, D. J., 2001. Ultracentrifugal studies of the effect of molecular crowding by trimethylamine N-oxide on the self-association of muscle glycogen phosphorylase b European Journal of Biochemistry. VOL 268, 506-513
HARDING, S.E and CHOWDHRY, B.Z., eds., 2001. Protein-Ligand Interactions: Structure and Spectroscopy Oxford University Press.
HARDING, S.E., ed., 2001. Biophysical Chemistry, Volume 93 (issues 2,3) pages 87-246: The Hydration Problem in Solution Biophysics Elsevier, Amsterdam.
WINZOR, D. J., CARRINGTON, L. E. and HARDING, S. E., 2001. Analysis of thermodynamic non-ideality in terms of protein solvation Biophysical Chemistry. VOL 93, 231-240
HARDING, S. E., 2001. The hydration problem in solution biophysics: an introduction Biophysical Chemistry. VOL 93, 87-91
PICOUT, D R, ROSS-MURPHY, S B, ERRINGTON, N and HARDING, S E, 2001. Pressure cell assisted solution characterization of polysaccharides. 1. Guar gum. Biomacromolecules. 2, 1301-1319
HARDING, S.E and CHOWDHRY, B.Z., 2001. Protein-ligand interactions and their analysis. In: HARDING, S.E and CHOWDHRY, B.Z., eds., Protein-Ligand Interactions: Hydrodynamics and Calorimetry Oxford University Press. 1-17
HARDING, S.E and CHOWDHRY, B.Z., eds., 2001. Protein-Ligand Interactions: Hydrodynamics and Calorimetry Oxford University Press.
AZHAKANANDAM, K., POWER, J. B., LOWE, K. C., COCKING, E. C., TONGDANG, T., JUMEL, K., BLIGH, H. F. J., HARDING, S. E. and DAVEY, M. R., 2000. Qualitative assessment of aromatic Indica rice (Oryza sativa L.): Proteins, lipids and starch in grain from somatic embryo- and seed-derived plants Journal of Plant Physiology. VOL 156, 783-789
CAVILL, P and HARDING, S.E. AND JESCH, J., 2000. Wirral and its Viking Heritage Nottingham: English Place-Name Society.
MORRIS, G. A., FOSTER, T. J. and HARDING, S. E., 2000. Further observations on the size, shape, and hydration of casein micelles from novel analytical ultracentrifuge and capillary viscometry approaches. Biomacromolecules. 1, 764-767
RAMESH, M., MITCHELL, J.R., JUMEL, K. and HARDING, S.E., 2000. What is the true amylose content of rice starch?. In: Gums and Stabilisers for the Food Industry 10 Royal Society of Chemistry, Cambridge, UK. 76-81
SOBOL, E., SVIRIDOV, A., OMEL'CHENKO, A., BAGRATASHVILI, V., KITAI, M., HARDING, S.E., JONES, N., JUMEL, K., MERTIG, M., POMPE, W., OVCHINNIKOV, Y., SHEKHTER, A. and SVISTUSHKIN, V., 2000. Laser reshaping of cartilage Biotechnology & Genetic Engineering Reviews. 17, 539-564
HARDING, S.E., 2000. Polysaccharides/Centrifugation. In: Encyclopaedia of Separation Science III Academic Press, Incorporated, Orlando, Florida, USA. 3921-3929
WALTERS, C., CLARKE, A., CLIFF, M. J., LUND, P. A. and HARDING, S. E., 2000. Trp203 mutation in GroEL promotes a self-association reaction: a hydrodynamic study European Biophysics Journal. VOL 29(PART 6), 420-428
DEACON, M.P, MCGURK, S, ROBERTS, C.J, WILLIAMS, P.M, TENDLER, S.J.B, DAVIES, M.C and DAVIS, S.S. AND HARDING, S.E., 2000. Atomic force microscopy of gastric mucin and chitosan mucoadhesive systems Biochemical Journal. 348, 557-563
THIESEN, A, JOHANN, C, DEACON, M.P and HARDING, S.E., 2000. Refractive Increment Data Book Nottingham University Press.
OMEL'CHENKO, A.I, SOBOL, E.N, SVIRIDOV, A.P, HARDING, S.E, JUMEL, K and WALKER, R. AND JONES, N., 2000. Optoacoustic monitoring of laser correction of the ear shape Kvantovaya Elektronika. 30, 1031-1033
MORRIS, G. A., FOSTER, T. J. and HARDING, S. E., 2000. The effect of the degree of esterification on the hydrodynamic properties of citrus pectin Food Hydrocolloids. VOL 14(NO 3), 227-236
HARDING, S.E., 2000. Locations and legends. In: CAVILL, P and HARDING, S.E. AND JESCH, J., eds., Wirral and its Viking Heritage English Place Name Society. 108-124
SOBOL, E.B, OMEL'CHENKO, A.I, SVIRIDOV, A.P, HARDING, S.E and JUMEL, K. AND JONES, N., 2000. Hydodynamic study of the behaviour of chondroitin sulphate under nondestructive laser irradiation of cartilage In: PROC. SPIE, 3914. 88-93
HARDING, S.E., 2000. Ingimund's Saga (1st edition, softback): Norwegian Wirral Countyvise Limited.
RAMESH, M., MITCHELL, J. R., JUMEL, K. and HARDING, S.E., 1999. Amylose Content of Rice Starch Starke (Starch). 51, 311-313
LAFITTE, D., HECK, A.J., HILL, T.J., JUMEL, K., HARDING, S.E. and DERRICK, P.J., 1999. Evidence of noncovalent dimerization of calmodulin European Journal of Biochemistry. 261, 337-344
TONGDANG, T., BLIGH, H. F. J., JUMEL, K. and HARDING, S. E., 1999. Combining sedimentation velocity with SEC-MALLS to probe the molecular structure of heterogeneous macromolecular systems that cannot be preparatively separated: application to three rice starches Progress in Colloid and Polymer Science. VOL 113, 185-191
PAVLOV, G. M., KORNEEVA, E. V., JUMEL, K., HARDING, S. E., MEIJER, E. W., PEERLINGS, H. W. I., STODDART, J. F. and NEPOGODIEV, S. A., 1999. Hydrodynamic properties of carbohydrate-coated dendrimers Carbohydrate Polymers. VOL 38, 195-202
DEACON, M.P., DAVIS, S.S., WHITE, R.J., NORDMAN, H., CARLSTEDT, I., ERRINGTON, N., ROWE, A.J. and HARDING, S.E., 1999. Are chitosan-mucin interactions specific to different regions of the stomach? Velocity ultracentrifugation offers a clue Carbohydrate Polymers. 38, 235-238
MORRIS, G. A., BUTLER, S. N. G., FOSTER, T. J., JUMEL, K. and HARDING, S. E., 1999. Elevated-temperature analytical ultracentrifugation of a low-methoxy polyuronide Progress in Colloid and Polymer Science. VOL 113, 205-208
HARDING, S.E., DAVIS, S.S., DEACON, M.P. and FIEBRIG, I., 1999. Biopolymer mucoadhesives Biotechnology and Genetic Engineering Reviews. 16, 41-86
HARDING, S.E., 1999. Protein hydrodynamics. In: ALLEN, G., ed., Protein: A Comprehensive Treatise 2. JAI Press Inc, Greenwich, USA. 271-305
HARDING, S.E., 1999. The Polysaccharide Biotechnology Royal Society of Chemistry Meeting 1997. Carbohydrate Polymers. 38, 193-194
HOLTHAM, C. A. M., JUMEL, K., MILLER, C. M., HARDING, S. E., BAUMBERG, S. and STOCKLEY, P. G., 1999. Probing Activation of the Prokaryotic Arginine Transcriptional Regulator Using Chimeric Proteins Journal of Molecular Biology. VOL 289, 707-727
HALL, D.R and HARDING, S.E. AND WINZOR, D.J., 1999. The correct analysis of low-speed sedimentation equilibrium distributions recorded by the Rayleigh interference optical system in a Beckman XL-I ultracentrifuge Progress in Colloid and Polymer Science. 113, 62-68
HARDING, S.E., 1999. Frictional coefficient, ratio. In: CREIGHTON, T.E., ed., Encyclopedia of Molecular Biology J. Wiley & Sons, New York. 942
AZIS, B. H., CHIN, B., DEACON, M. P., HARDING, S. E. and PAVLOV, G. M., 1999. Size and shape of inulin in dimethyl sulphoxide solution Carbohydrate Polymers. VOL 38, 231-234
PAVLOV, G.M., HARDING, S.E. and ROWE, A.J., 1999. Normalized scaling relations as a natural classification of linear macromolecules according to size Progress in Colloid and Polymer Science. 113, 76-80
CARRASCO, B., GARCIA DE LA TORRE, J., BYRON, O., KING, D., WALTERS, C., JONES, S. and HARDING, S. E., 1999. Novel Size-Independent Modeling of the Dilute Solution Conformation of the Immunoglobulin IgG Fab' Domain Using SOLPRO and ELLIPS Biophysical Journal. VOL 77, 2902-2910
JONES, S., THORNTON, J.M., WINZOR, D.J., HARDING, S.E. and HORTON, J.C., 1999. COVOL: An interactive program for evaluating second virial coefficients from the triaxial shape or dimensions of rigid macromolecules Biophysical Journal. 76, 2432-2438
GARCIA DE LA TORRE, J., HARDING, S. E. and CARRASCO, B., 1999. Calculation of NMR relaxation, covolume, and scattering-related properties of bead models using the SOLPRO computer program European Biophysics Journal. VOL 28, 119-132
HARDING, S.E., 1999. Diffusion. In: CREIGHTON, T.E., ed., Encyclopedia of Molecular Biology J. Wiley & Sons, New York. 674-678
SCHIFFMANN, D.A, WHITE, J.H.M, COOPER, A, NUTLEY, M.A, HARDING, S.E, JUMEL, K, SOLARI, R and RAY, K.P. AND GAY, N.J., 1999. Formation and biochemical characterisation of tube/pelle death domain complexes: Critical regulators of postreceptor signalling by the Drosophila Toll receptor Biochemistry. 38, 11722-11733
HARDING, S.E., ed., 1999. Carbohydrate Polymers, Volume 38 (issue 3), pages 193-286: Polysaccharide Biotechnology Elsevier.
WALTERS, C., CLIFF, M., CLARKE, A. and HARDING, S. E., 1999. Nonequilibrium self-association of a cpn60 chaperonin induced by tryptophan mutation Progress in Colloid and Polymer Science. VOL 113, 216-220
BYRON, O., HARDING, S.E., KING, D., JONES, S., WALTERS, C., CARRASCO, B. and GARCIA DE LA TORRE, J., 1999. Novel size-independent modelling of the dilute solution conformation of the immunoglobin IgG Fab' domain using SOLPRO and ELLIPS Biophysical Journal. 77, 2902-2910
MORRIS, G.A., EBRINGEROVA, A., HARDING, S.E. and HROMADKOVA, Z., 1999. UV tagging leaves the structure integrity of an arabino-(4-0-methylglucurono)xylan polysaccharide unaffected Progress in Colloid and Polymer Science. 113, 201-204
TAYLOR, A. M., BOULTER, J., HARDING, S. E., COELFEN, H. and WATTS, A., 1999. Hydrodynamic Properties of Human Erythrocyte Band 3 Solubilized in Reduced Triton X-100 Biophysical Journal. VOL 76, 2043-2055
KIRKITADZE, M.D, JUMEL, K, HARDING, S.E, DRYDEN, D.T.F, KRYCH, M and ATKINSON, J.P. AND BARLOW, P.N., 1999. Combining ultracentrifugation with fluorescence to follow the unfolding of modules 16-17 of complement receptor type 1 Progress in Colloid and Polymer Science. 113, 164-167
HARDING, S.E., 1999. Photon correlation spectroscopy. In: CREIGHTON, T.E., ed., Encyclopedia of Molecular Biology J. Wiley and Sons, New York. 1847-1849
JUMEL, K., WINZOR, D. J. and HARDING, S. E., 1998. Advances in Ultracentrifuge Analysis: Introductory remarks Biochemical Society Transactions. VOL 26, 715
PAVLOV, G. M., KORNEEVA, E. V., NEPOGOD EV, S. A., JUMEL, K. and HARDING, S. E., 1998. Translational and Rotational Friction of Lactodendrimer Molecules in Solution Polymer Science Series A. VOL 40, 1282-1289
JUMEL, K., HARDING, S.E. and WINZOR, D.J., 1998. Analytical ultracentrifugation at Nottingham, Leicester and the National Centre for Macromolecular Hydrodynamics Biochemist. October, 53-55
DEACON, M. P., DAVIS, S. S., WAITE, J. H. and HARDING, S. E., 1998. Structure and mucoadhesion of mussel glue protein in dilute solution Biochemistry. VOL 37, 14108-14112
HARDING, S.E., 1998. Dilute solution viscometry of food biopolymers. In: HILL, S.E, LEDWARD, D.A and MITCHELL, J.R., eds., Functional Properties of Food Macromolecules Aspen Publishers Inc, New York, USA. 1-49
HARDING, S.E., CARRASCO, B. and GARCIA DE LA TORRE, J., 1998. New strategies for modelling of antibody structure in dilute solution Glycobiology. 8, 114
PAVLOV, G. M., KORNEEVA, E. V., VIKHOREVA, G. A. and HARDING, S. E., 1998. Hydrodynamic and Molecular Characteristics of Carboxymethylchitin in Solution Polymer Science Series A. VOL 40, 1275-1281
GARCIA DE LA TORRE, J., HARDING, S. E. and CARRASCO, B., 1998. Advances in modelling solution properties of macromolecules and particles Biochemical Society Transactions. VOL 26, 716-720
CARRASCO, B., GARCIA DE LA TORRE, J. and HARDING, S. E., 1998. Models for the multisubunit conformation of oil-seed globulins Biochemical Society Transactions. VOL 26, 721-725
CARRASCO, B., HARDING, S.E. and GARCIA DE LA TORRE, J., 1998. Usefulness of the bead model algorithm SOLPRO for modelling the conformation of seed globulins. In: Plant Proteins from European Crops. Food and Non-Food Applications Springer-Verlag, Heidelberg. 153-155
DODD, S., PLACE, G. A., HALL, R. L. and HARDING, S. E., 1998. Hydrodynamic properties of mucins secreted by primary cultures of Guinea-pig tracheal epithelial cells: determination of diffusion coefficients by analytical ultracentrifugation and kinetic analysis of mucus gel hydration and dissolution European Biophysics Journal. VOL 28, 38-47
CARRASCO, B., HARDING, S. E. and GARCIA DE LA TORRE, J., 1998. Bead modeling using HYDRO and SOLPRO of the conformation of multisubunit proteins: sunflower and rape-seed 11S globulins Biophysical Chemistry. VOL 74, 127-133
HARDING, S.E, JUMEL, K and WINZOR, D.J., eds., 1998. Biochemical Society Transactions, Vol. 26, pages 715-769: Advances in Ultracentrifugation Analysis The Biochemical Society.
HARDING, S.E., 1998. Mucin-biopolymer interactions for mucoadhesion (Abstract 53) Glycobiology. 8(11),
HARDING, S.E. AND JUMEL, K., 1998. Light Scattering. In: COLIGAN, J.E, DUNN, B, PLOEGH, H.L and SPEICHER, D.W. AND WINGFIELD, P.T., eds., Current Protocols in Protein Science J. Wiley & Sons, New York. 7.8.1 - 7.8.14
CÖLFEN, H., BOULTER, J. M., HARDING, S. E. and WATTS, A., 1998. Ultracentrifugation studies on the transmembrane domain of the human erythrocyte anion transporter Band 3 in the detergent C~1~2E~8 European Biophysics Journal. VOL 27, 651-655
EDITORIAL (S. HARVEY), 1998. The Spin Doctors: article about the work of Stephen Harding and the NCMH University of Nottingham Newsletter.
GOLBIK, R., ZAHN, R., HARDING, S. E. and FERSHT, A. R., 1998. Thermodynamic Stability and Folding of GroEL Minichaperones Journal of Molecular Biology. VOL 276, 505-515
PAVLOV, G. M., KORNEEVA, E. V., HARDING, S. E. and VICHOREVA, G. A., 1998. Dilute solution properties of carboxymethylchitins in high ionic-strength solvent Polymer. VOL 39, 6951-6961
FIEBRIG, I., VÅRUM, K. M., HARDING, S. E., DAVIS, S. S. and STOKKE, B. T., 1997. Colloidal gold and colloidal gold labelled wheat germ agglutinin as molecular probes for identification in mucin/chitosan complexes Carbohydrate Polymers. VOL 33, 91-99
JUMEL, K., GEIDER, K. and HARDING, S. E., 1997. The solution molecular weight and shape of the bacterial exopolysaccharides amylovoran and stewartan International Journal of Biological Macromolecules. VOL 20, 251-258
JUMEL, K., FOGG, F. J. J., HUTTON, D. A., PEARSON, J. P., ALLEN, A. and HARDING, S. E., 1997. A polydisperse linear random coil model for the quaternary structure of pig colonic mucin European Biophysics Journal. VOL 25, 477-480
THOMAS, R.M., ZAMPIERI, A., JUMEL, K. and HARDING, S.E., 1997. A trimeric, alpha-helical, coiled coil peptide: association stoichiometry and interaction strength by analytical ultracentrifugation European Biophysics Journal. 25, 405-410
GARCIA DE LA TORRE, J., CARRASCO, B. and HARDING, S. E., 1997. SOLPRO: theory and computer program for the prediction of SOLution PROperties of rigid macromolecules and bioparticles European Biophysics Journal. VOL 25, 361-372
HARDING, S. E., HORTON, J. C. and WINZOR, D. J., 1997. COVOL: an answer to your thermodynamic non-ideality problems? Biochemical Society Transactions. VOL 26, 737-740
HARDING, S.E., 1997. Characterisation of chitosan-mucin complexes by sedimentation velocity analytical ultracentrifugation. In: MUZZARELLI, R.A.A and PETER, M.G., eds., Chitin Handbook European Chitin Society, Lyon, France. 457-466
CÖLFEN, H., HARDING, S.E., WILSON, E.K., SCRUTTON, N.S. and WINZOR, D.J., 1997. Low temperature solution behaviour of Methylophilus methylotrophus electron transferring flavoprotein: a study by analytical ultracentrifugation European Biophysics Journal. 25, 411-416
TOMBS, M.P and HARDING, S.E., 1997. An Introduction to Polysaccharide Biotechnology Taylor & Francis, London.
CÖLFEN, H. and HARDING, S. E., 1997. MSTARA and MSTARI: interactive PC algorithms for simple, model independent evaluation of sedimentation equilibrium data European Biophysics Journal. VOL 25, 333-346
BYRON, O. and HARDING, S. E., 1997. New developments in analytical ultracentrifugation and related macromolecular modelling techniques European Biophysics Journal. VOL 25(NUMBER 5/6), 305-306
SILKOWSKI, H., DAVIS, S.J., BARCLAY, A.N., ROWE, A.J., HARDING, S.E. and BYRON, O., 1997. Characterisation of the low affinity interaction between rat cell adhesion molecules CD2 and CD48 by analytical ultracentrifugation European Biophysics Journal. 25, 455-462
HARDING, S. E., DAY, K., DHAMI, R. and LOWE, P. M., 1997. Further observations on the size, shape and hydration of kappa-carrageenan in dilute solution Carbohydrate Polymers. VOL 32, 81-87
HARDING, S.E., 1997. Hydrodynamic properties of proteins (Chapter 9). In: CREIGHTON, T.E., ed., Protein Structure: A Practical Approach IRL Press, Oxford, UK. 219-251
DEACON, M. P., DAVIS, S. S., WHITE, R., WAITE, J. H. and HARDING, S. E., 1997. Monomeric behaviour of Mytilus edulin (mussel) glue protein in dilute solution Biochemical Society Transactions. VOL 25, 422S
MORGAN, P. J. and HARDING, S. E., 1997. Nitrite reductase from Achromobacter xylosoxidans forms stable trimers in dilute solution Biochemical Society Transactions. VOL 25, 40S
HARDING, S. E., HORTON, J. C. and COLFEN, H., 1997. The ELLIPS suite of macromolecular conformation algorithms European Biophysics Journal. VOL 25, 347-360
HARDING, S.E and BYRON, O., eds., 1997. European Biophysics Journal, Volume 25 (number 5-6), pages 305-487: New Developments in Analytical Ultracentrifugation and Related Macromolecular Modelling Techniques Springer-Verlag.
WILSON, E. K., SCRUTTON, N. S., COELFEN, H., HARDING, S. E., JACOBSEN, M. P. and WINZOR, D. J., 1997. An ultracentrifugal approach to quantitative characterization of the molecular assembly of a physiological electron-transfer complex - The interaction of electron-transferring flavoprotein with trimethylamine dehydrogenase European Journal of Biochemistry. VOL 243, 393-399
HARDING, S.E., 1997. Microbial laser light scattering Biotechnology and Genetic Engineering Reviews. 14, 145-164
DEAN, J. L. E., CÖLFEN, H., HARDING, S. E., RICE, D. W. and ENGEL, P. C., 1997. Alteration of the quaternary structure of glutamate dehydrogenase from Clostridium symbiosum by a single mutation distant from the subunit interfaces European Biophysics Journal. VOL 25, 417-422
HARDING, S.E., 1997. The intrinsic viscosity of biological macromolecules. Progress in measurement, interpretation and application to structure in dilute solution Progress in Biophysics and Molecular Biology (ed., Blundell, T.L.). 68 No. 2/3, 207-262
PAVLOV, G. M., ROWE, A. J. and HARDING, S. E., 1997. Conformation zoning of large molecules using the analytical ultracentrifuge Trends in Analytical Chemistry. VOL 16, 401-404
HAGAN, S. A., COOMBES, A. G. A., GARNETT, M. C., DUNN, S. E., DAVIES, M. C., ILLUM, L., DAVIS, S. S., HARDING, S. E., PURKISS, S. and GELLERT, P. R., 1996. Polylactide-Poly(ethylene glycol) Copolymers as Drug Delivery Systems. 1. Characterization of Water Dispersible Micelle-Forming Systems LANGMUIR. VOL 12(NUMBER 9), 2153-2161
MOLINA-GARCIA, A.D., DE PIERI, L.A., LUDLOW, I., WAITES, W.M., GARCIA DE LA TORRE, J. and HARDING, S.E., 1996. Comparison of quasi-elastic light scattering and laser diffractometry as nondestructive probes into the structure of Bacillus sphaericus spores produced at different temperatures Applied and Environmental Microbiology. 62, 1699-1704
EASA, A. M., ARMSTRONG, H. J., MITCHELL, J. R., HILL, S. E., HARDING, S. E. and TAYLOR, A. J., 1996. Maillard induced complexes of bovine serum albumin - a dilute solution study International Journal of Biological Macromolecules. VOL 18, 297-301
JUMEL, K., HARDING, S. E., MITCHELL, J. R., TO, K.-M., HAYTER, I., O'MULLANE, J. E. and WARD-SMITH, S., 1996. Molar mass and viscometric characterisation of hydroxypropylmethyl cellulose Carbohydrate Polymers. VOL 29, 105-109
JUMEL, K., HARDING, S.E. and MITCHELL, J.R., 1996. Effect of gamma irradiation on the macromolecular integrity of guar gum Carbohydrate Research. 282, 223-236
SOUSA, I. M. N., MORGAN, P. J., MITCHELL, J. R., HARDING, S. E. and HILL, S. E., 1996. Hydrodynamic characterization of lupin proteins: Solubility, intrinsic viscosity, and molar mass Journal of Agricultural and Food Chemistry. VOL 44, 3018-3021
JUMEL, K., FIEBRIG, I. and HARDING, S.E., 1996. Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromotography/multi angle laser light scattering International Journal of Biological Macromolecules. 18, 133-139
PINTAR, A., HENSMANN, M., JUMEL, K., PITKEATHLY, M., HARDING, S. E. and CAMPBELL, I. D., 1996. Solution studies of the SH2 domain from the fyn tyrosine kinase: secondary structure, backbone dynamics and protein association European Biophysics Journal. VOL 24, 371-380
HARDING, S.E., BERTH, G., HARTMANN, J., JUMEL, K., COLFEN, H. and CHRISTENSEN, B.E., 1996. Physicochemical studies on Xylinan (Acetan). III. Hydrodynamic characterization by analytical ultracentrifugation and dynamic light scattering Biopolymers. 39, 729-736
HOLLOWAY, D.E., HARDING, S.E. and MARSH, E.N., 1996. Adenosylcobalamin-dependent glutamate mutase: properties of a fusion protein in which the cobalmin-binding subunit is linked to the catalytic subunit Biochemical Journal. 320, 825-830
KORNEEVA, E.V., VICHOREVA, G.A., HARDING, S.E. and PAVLOV, G.M., 1996. Hydrodynamic study of carboxymethyl chitin Abstracts of Papers of the American Chemical Society. 212 (part 1), 75-cell
COLFEN, H., HARDING, S.E., WILSON, E.K., PACKMAN, L.C. and SCRUTTON, N.S., 1996. Homodimeric and expanded behaviour of trimethylamine dehydrogenase in solution at different temperatures European Biophysics Journal. 24, 159-164
CÖLFEN, H., HARDING, S. E., VÅRUM, K. M. and WINZOR, D. J., 1996. A study by analytical ultracentrifugation on the interaction between lysozyme and extensively deacetylated chitin (chitosan) Carbohydrate Polymers. VOL 30, 45-53
BERTH, G., DAUTZENBERG, H., CHRISTENSEN, B. E., HARDING, S. E., ROTHER, G. and SMIDSROED, O., 1996. Static Light Scattering Studies on Xanthan in Aqueous Solutions Macromolecules. VOL 29, 3491-3498
COELFEN, H., HARDING, S. E., BOULTER, J. M. and WATTS, A., 1996. Hydrodynamic examination of the dimeric cytoplasmic domain of the human erythrocyte anion transporter, band 3 Biophysical Journal. VOL 71, 1611-1615
FOGG, F.J.J, HUTTON, D.A, JUMEL, K, PEARSON, J.P and HARDING, S.E. AND ALLEN, A., 1996. Characterization of pig colonic mucins Biochemical Journal. 316, 937-942
CÖLFEN, H., HARDING, S. E. and VÅRUM, K. M., 1996. Investigation, using analytical ultracentrifugation, of the effect of the incorporation of the fluorophore 9-anthraldehyde on two chitosans of differing degrees of acetylation Carbohydrate Polymers. VOL 30, 55-60
GADHAVI, P., MORGAN, P. J., ALEFOUNDER, P. and HARDING, S. E., 1996. A physico-chemical investigation of the self-association of the DNA binding domain of the yeast transcriptional activator GAL 4 European Biophysics Journal. VOL 24, 405-412
PASQUO, A., BRITTON, K.L., STILLMAN, T.J., RICE, D.W., COLFEN, H., HARDING, S.E., SCANDURRA, R. and ENGEL, P.C., 1996. Construction of a dimeric form of glutamate dehydrgenase from clostridium symbiosum by site-directed mutagenesis Biochimica et Biophysica Acta. 1297, 149-158
SOUSA, I.M., MITCHELL, J.R., HILL, S.E. and HARDING, S.E., 1995. Intrinsic viscosity and Mark-Houwink parameter of lupin proteins in aqueous solutions. Proceedings of 30eme Colloque Annuel du groupe Francais de Rheologie, Bordeaux, 13-15 September 1995 Les Cahiers de Rheologie. 14, 139-148
ROBERTS, C. J., SHIVJI, A., DAVIES, M. C., DAVIS, S. S., FIEBRIG, I., HARDING, S. E., TENDLER, S. J. B. and WILLIAMS, P. M., 1995. A study of highly purified pig gastric mucin by scanning tunnelling microscopy Protein and Peptide Letters. VOL 2, 409-414
JUMEL, K., WILSON, S., SMITH, M. and HARDING, S.E., 1995. Investigations of the oligomeric state of the 42kDa repressor isoform from the Streptmyces temperate bacteriophage oC3 Progress in Colloid and Polymer Science. 99, Nov-16
DHAMI, R., HARDING, S. E., JONES, T. and HUGHES, T., 1995. Physico-chemical studies on a commercial food-grade xanthan - I. Characterisation by sedimentation velocity, sedimentation equilibrium and viscometry Carbohydrate Polymers. VOL 27, 93-99
HARDING, S.E. and ROWE, A.J., 1995. Active enzyme centrifugation (Chapter 2F). In: , ed., Enzymology Labfax Bios Scientific Publishers Ltd, Oxford, UK. 58-61
RIGHETTI, P.G., HEINEVETTER, L., COLFEN, H. and HARDING, S.E., 1995. Alternative Bioseparation Techniques (Chapter 10). In: , ed., High performance Liquid Chromotography Methods in Biotechnology John Wiley & Sons, Chichester, UK.
HARDING, S.E, HILL, S.E and MITCHELL, J.R., eds., 1995. Biopolymer Mixtures Nottingham University Press.
HARDING, S.E., 1995. Book review: "Rheology of Industrial Polysaccharides: Theory and Applications" (R. Lapasin and S. Pricl. eds.) Carbohydrate Research. 237, c1-c3
HARDING, S.E., 1995. Ultracentrifugation of food biopolymers. In: DICKINSON, E., ed., New physico-techniques for the charcterisation of complex food systems Blackie Academic & Professional, London, UK. 117-146
FIEBRIG, I., HARDING, S.E., ROWE, A.J., HYMAN, S.C. and DAVIS, S.S., 1995. Transmission electron microscopy studies on pig gastric mucin and its interactions with chitosan Carbohydrate Polymers. 28, 239-244
DHAMI, R., HARDING, S. E., ELIZABETH, N. J. and EBRINGEROVA, A., 1995. Hydrodynamic characterisation of the molar mass and gross conformation of corn cob heteroxylan AGX Carbohydrate Polymers. VOL 28, 113-119
KENDREW, S.G., HARDING, S.E., HOPWOOD, D.A., NEIL, E. and MARSH, G., 1995. Identification of a flavin:NADH oxidoreductase involved in the biosynthesis of actinorhodin (Purification and characterization of the recombinant enzyme) Journal of Biological Chemistry. 270, 17339-17343
HARDING, S. E. and CÖLFEN, H., 1995. Inversion formulae for ellipsoid of revolution macromolecular shape functions Analytical Biochemistry. VOL 228, 131-142
SEIFERT, A., HEINEVETTER, L., COLFEN, H. and HARDING, S.E., 1995. Characterisation of gliadin-galactomannan incubation mixtures by analytical ultracentrifugation. Part l: Sedimentation velocity Carbohydrate Polymers. 28, 325-332
FOSSATI, G., LUCIETTO, P., GIULIANI, P., COATES, A.R., HARDING, S.E., COLFEN, H., LEGNAME, G., CHAN, E., ZALIANI, A. and MASCAGNI, P., 1995. Mycobacterium tuberculosis chaperonin 10 forms stable tetrameric and heptameric structures. Implications for its diverse biological activities Journal of Biological Chemistry. 270, 26159-26167
SILKOWSKI, H., BYRON, O., DAVIS, S.J., BARCLAY, A.N., DAVIES, E.A., ROWE, A.J. and HARDING, S.E., 1995. Estimation of the dissacociation constant of the cell adhesion molecules srCD2 and srCD48 using analytical ultracentrifugation Biochemical Society Transactions. 23, 435S
HARDING, S. E., 1995. On the hydrodynamic analysis of macromolecular conformation Biophysical Chemistry. VOL 55, 69-93
HARDING, S.E., 1995. Some recent developments in the analytical ultracentrifugation of food proteins Nahrung. 39, 375-395
FIEBRIG, I., DAVIS, S.S. and HARDING, S.E., 1995. Methods used to develop mucoadhesive drug delivery systems: Bioadhesion in the gastrointestinal tract (Chapter 18). In: , ed., Biopolymer Mixtures Nottingham University Press, Nottingham.
COELFEN, H. and HARDING, S. E., 1995. A study on Schlieren patterns derived with the Beckman Optima XL-A UV-absorption optics Progress in Colloid and Polymer Science. VOL 99, 167-186
HARDING, S. E., 1995. Some recent developments in the size and shape analysis of industrial polysaccharides in solution using sedimentation analysis in the analytical ultracentrifuge Carbohydrate Polymers. VOL 28, 227-237
DHAMI, R., COLFEN, H. and HARDING, S.E., 1995. A comparitive "Schlieren" study of the sedimentation behaviour of three polysaccharides using the Beckman Optima XL-A and Model E analytical centrifuges Progress in Colloid and Polymer Science. 99, 188-193
COELFEN, H., HUSBANDS, P. and HARDING, S. E., 1995. Alternative light sources for the Schlieren optical system of analytical ultracentrifuges Progress in Colloid and Polymer Science. VOL 99, 193-198
SOUSA, I.M., BEIRAO DA COSTA, M.L., HILL, S.E., MITCHELL, J.R. and HARDING, S.E., 1994. Functional properties of Lupinus luteus proteins. In: YANO, T, MATSUNO, R and NAKAMURA, eds., Functional properties of Lupinus luteus proteins. Developments in Food Engineering. Proceedings of the 6th International Congress on Engineering and Food. Eds Yano,T, Matsuno,R and Nakamura,K. Pages 206-208: Proceedings of the 6th International Congress on Engineering and Food Blackie Academic and Professional. 206-208
ONG, M.H., JUMEL, K., TOKARCZUK, P.F., BLANSHARD, J.M. and HARDING, S.E., 1994. Simultaneous determinations of the molecular weight distributions of amyloses and the fine structures of amylopectins of native starches Carbohydrate Research. 260, 99-117
KELLY, R., GUDO, E. S., MITCHELL, J. R. and HARDING, S. E., 1994. Some observations on the nature of heated mixtures of bovine serum albumin with an alginate and a pectin Carbohydrate Polymers. VOL 23, 115-120
HARDING, S.E., 1994. Determination of absolute molecular weights using sedimentation equilibrium analytical ultracentrifugation. In: , ed., Methods in Molecular Biology 22. 75-84
FIEBRIG, I., HARDING, S.E. and DAVIS, S.S., 1994. Sedimentation analysis of potential interactions between mucins and a putative bioadhesive polymer Progress in Colloid and Polymer Science. 94, 66-73
HARDING, S.E., 1994. The sedimentation equilibrium analysis of polysaccharides and mucins: A guided tour of problem solving for difficult heterogeneous systems. In: Modern Analytical Ultracentrifugation 22-Microscopy, Opt... 315-341
FIEBRIG, I, HARDING, S.E, STOKKE, B.T, VARUM, K.M and JORDAN, D. AND DAVIS, S.S., 1994. The potential of chitosan as a mucoadhesive drug carrier: studies on its interaction with pig gastric mucin on a molecular level Eur. J. Pharmaceut. Sci.. 2, 185
FOGG, F.J., ALLEN, A., HARDING, S.E. and PEARSON, J.P., 1994. The structure of secreted mucins isolated from the adherent mucus gel: comparison with the gene products Biochemical Society Transactions. 22, 229S
HARDING, S.E., 1994. Determination of macromolecular heterogeneity, shape and interactions using sedimentation velocity analytical ultracentrifugation. In: Methods in Molecular Biology 22. 61-73
HARDING, S.E., 1994. Shapes and sizes of food polysaccharides by sedimentation analysis - recent developments Gums and Stabilisers for the Food Industry. 7, 55-65
REILLY, A., MORGAN, P.J., DAVIS, K., KELLY, S.M., GREENE, J., ROWE, A.J., HARDING, S.E., PRICE, N.C., COGGINS, J.R. and KLEANTHOUS, C., 1994. Product induced stabilisation of tertiary and quaternary structure in E.coli dehydroquinase Journal of Biological Chemistry. 269, 5523-5526
HARDING, S. E., 1994. Physical Techniques for the Study of Food Biopolymers (S.B. Ross-Murphy, ed.) Trends in Food Science and Technology. VOL 5, 126
ISRAILIDES, C, SCANLON, B, SMITH, A, HARDING, S.E and JUMEL, K., 1994. Characterization of pullulans produced from agro-industrial waste Carbohydrate Polymers. 25, 203-209
HARDING, S.E., 1994. Determination of diffusion coefficients of macromolecules by Dynamic Light Scattering (DLS). In: Methods in Molecular Biology 22. 97-108
HARDING, S. E., 1994. Sedimentation equilibrium analysis of glycopolymers Progress in Colloid and Polymer Science. 94, 54-65
HARDING, S. E., 1994. The analytical ultracentrifuge spins again Trends in Analytical Chemistry. VOL 13, 439-446
HARDING, S.E., 1994. Classical light scattering for the determination of absolute molecular weights and gross conformation of biological macromolecules. In: Methods in Molecular Biology 22. 85-95
HARDING, S.E., 1994. The sedimentation equilibrium analysis of polysaccharides and mucins: A guided tour of problem solving for difficult heterogeneous systems. In: SCHUSTER, T.M and LAUE, T.M., eds., Modern Analytical Ultracentrifugation Birkhäuser, Boston USA. 315-341
HARDING, S.E., JUMEL, K., KELLY, R., GUDO, E., HORTON, J.C. and MITCHELL, J.R., 1993. The structure and nature of protein-polysaccharide complexes. In: Food Proteins: Structure and Functionality 8. 216-226
ERRINGTON, N., HARDING, S. E., VARUM, K. M. and ILLUM, L., 1993. Hydrodynamic characterization of chitosans varying in degree of acetylation International Journal of Biological Macromolecules. VOL 15, 113-117
HARDING, S.E., 1993. Cinderella will go to the ball: Analytical ultracentrifugation has revived as a tool for protein characterisation Laboratory News. March, 20
HARDING, S.E. and BERTH, G., 1993. News and Views: Symposium on conformational studies of oligosaccharides, polysaccharides and glycoconjucates (Le Croisic, Loire Atlantique, France, 29 June - 2 July 1992) Carbohydrate Polymers. 20, 63-64
HARDING, S.E., 1993. Examining Cinderella (The 7th Harden discussion meeting on "Physical Methods for Glycopolymer Characterisation", Eynsham Hall, 11-13 September 1992) Biochemist. Jun/July, 30-31
MARSH, E.N. and HARDING, S.E., 1993. Methylmalonyl-CoA mutase from Propionibacterium shermanii: characterisation of the cobalmin-inhibited form and subunit-cofactor interactions studied by analytical ultracentrifugation Biochemical Journal. 290, 551-555
HARDING, S.E., 1993. Analytical ultracentrifugation and the genetic engineering of macromolecules Biotechnology and Genetic Engineering Reviews. 11, 317-356
DJABALLAH, H., ROWE, A. J., HARDING, S. E. and RIVETT, A. J., 1993. The multicatalytic proteinase complex (proteasome): structure and conformational changes associated with changes in proteolytic activity Biochemical Journal. VOL 292, 857-882
JUMEL, K, HARDING, S.E, MITCHELL, J.R and DICKINSON, E., 1993. Evidence for protein-polysaccharide complex formation as a result of dry-heating of mixtures. In: DICKINSON, E and WALSTRA, P, eds., Food Colloids and Polymers: Stability and Mechanical Properties Royal Society of Chemistry, Cambridge. 156-160
MARSH, N. AND HARDING, S.E., 1993. Methylmalonyl mutase from P. shermanii: characterisation of the cobalamin-inhibited form and subunit-cofactor interactions studied by analytical ultracentrifugation Biochemical Journal. 290, 551-555
KING, D.J., BYRON, O., MOUNTAIN, A., WEIR, N., HARVEY, A., LAWSON, A.D., PROUDFOOT, K.A., BALDOCK, D., HARDING, S.E., YARRANTON, G.T. and OWENS, R.J., 1993. Expression, purification and characterization of B72.3 Fv fragments Biochemical Journal. 290, 723-729
MANNION, R.O., MELIA, C.D., LAUNAY, B., CUVELIER, G., HILL, S.E., HARDING, S.E. and MITCHELL, J.R., 1992. Xanthan/locust bean gum interactions at room temperature Carbohydrate Polymers. 19, 91-97
ERRINGTON, N., HARDING, S.E., ILLUM, L. and SCHACT, E.H., 1992. Physico-chemical studies on di-iodotyrosine dextran Carbohydrate Polymers. 18, 611-613
ERRINGTON, N., HARDING, S.E. and ROWE, A.J., 1992. Use of scanning absorption optics for sedimentation equilibrium analysis of labelled polysaccharides: molecular weight of blue dextran Carbohydrate Polymers. 17, 151-154
ROWE, A.J, WYNNE-JONES, S and THOMAS, D.G. AND HARDING, S.E., 1992. Methods of off-line analysis of sedimentation velocity and sedimentation equilibrium patterns. In: HARDING, S.E and ROWE, A.J. AND HORTON, J.C., eds., Analytical Ultracentrifugation in Biochemistry and Polymer Science Royal Society of Chemistry, Cambridge. 49-62
HARDING, S.E., 1992. Sedimentation analysis of polysaccharides. In: HARDING, S.E and ROWE, A.J. AND HORTON, J.C., eds., Analytical Ultracentrifugation in Biochemistry and Polymer Science Royal Society of Chemistry, Cambridge. 495-516
HARDING, S.E., 1992. Total intensity and quasi-elastic light scattering applications in microbiology. In: HARDING, S.E and SATTELLE, D.B. AND BLOOMFIELD, V.A. EDS, eds., Laser Light Scattering in Biochemistry Royal Society of Chemistry, Cambridge. 365-386
HARDING, S.E., ROWE, A.J. and HORTON, J.C., eds., 1992. Analytical Ultracentrifugation in Biochemistry and Polymer Science Royal Society of Chemistry, Cambridge.
EDITORIAL, 1992. Chemistry in Britain Feature on P.J. Lillford and S.E. Harding Chemistry in Britain. 28, 370
HARDING, S.E and HORTON, J.C. AND MORGAN, P.J., 1992. MSTAR: A FORTRAN algorithm for the model independent molecular weight analysis of macromolecules using low speed or high speed sedimentation equilibrium. In: HARDING, S.E and ROWE, A.J. AND HORTON, J.C., eds., Analytical Ultracentrifugation in Biochemistry and Polymer Science Royal Society of Chemistry, Cambridge. 275-294
MOLINA-GARCIA, A. D., HARDING, S. E., GUILLERMO DIAZ, F., GARCIA DE LA TORRE, J., ROWITCH, D. and PERHAM, R.N., 1992. Effect of coat protein mutations in bacteriophage fd studied by sedimentation analysis Biophysical Journal. VOL 63, 1293-1298
MITCHELL, J.R, HILL, S.E and JUMEL, K. AND HARDING, S.E., 1992. The use of anti-oxidants to control viscosity and gel strength loss on heating of galactomannan systems Gums and Stabilisers for the Food Industry. 6, 303-309
MORGAN, P.J., BYRON, O. and HARDING, S.E., 1992. The solution conformation of novel antibody fragments studied using analytical ultracentrifugation Discovery. 1-4
KLEANTHOUS, C, DEKA, R, DAVIS, K, KELLY, S.M, COOPER, A, HARDING, S.E, PRICE, N.C and HAWKINS, A.R. AND COGGINS, J.R., 1992. A comparison of the enzymological and biophysical properties of two distinct classes of dehydrogenase enzymes Biochemical Journal. 282, 687-695
HARDING, S.E, SATTELLE, D.B and BLOOMFIELD, V.A., eds., 1992. Laser Light Scattering in Biochemistry Royal Society of Chemistry, Cambridge.
HARDING, S.E., 1992. News and Views: First Advanced Course on Alginates and their Applications Carbohydrate Polymers. 17, 155
MORGAN, P.J and BYRON, O. AND HARDING, S.E., 1992. Ultracentrifugation: The solution conformation of novel antibody fragments using analytical ultracentrifugation. In: , ed., Technical Information (Beckman Instruments, Palo Alto) DS-83.4
HORTON, J.C., HARDING, S.E. and MITCHELL, J.R., 1991. Gel permeation chromotography - multi angle laser light scattering characterization of the molecular mass distribution of "Pronova" sodium alginate Biochemical Society Transactions. 19, 510-511
HARDING, S.E., BERTH, G., BALL, A., MITCHELL, J.R. and GARCIA DE LA TORRE, J., 1991. The molecular weight distribution and conformation of citrus pectins in solution studied by hydrodynamics Carbohydrate Polymers. 16, 1-15
HORTON, J.C., HARDING, S.E., MITCHELL, J.R. and MORTON-HOLMES, D.F., 1991. Thermodynamic non-ideality of dilute solutions of sodium alginate studied by sedimentation equilibrium ultracentrifugation Food Hydrocolloids. 5, 125-127
MANNION, R.O., MELIA, C.D., MITCHELL, J.R. and HARDING, S.E., 1991. Effect of xanthan/locust bean gum synergy on ibuprofen release from hydrophilic matrix tablets Journal of Pharmacy and Pharmacology. 43, 79P
HARDING, S.E., SATTELLE, D.B. and BLOOMFIELD, V.A., 1991. Laser light scattering in Biochemistry: Introductory remarks Biochemical Society Transactions. 19, 477
HARDING, S.E., 1991. Total intensity and Quasi-elastic light scattering applications in microbiology Biochemical Society Transactions. 19, 499
HARDING, S.E., 1991. Books in Brief: An Introduction to Centrifugation, by T.C. Ford & J.N. Graham Trends. Biochem. Sci. 16, 392
HARDING, S.E., ed., 1991. Biochemical Society Transactions, Vol. 19, pages 477-516: Laser Light Scattering in Biochemistry The Biochemical Society.
HARDING, S.E., VÅRUM, K.M., STOKKE, B.T. and SMIDSRØD, O., 1991. Molecular weight determination of polysaccharides. In: , ed., Advances in Carbohydrate Analysis 63-144
SIMPKIN, N.J., HARDING, S.E. and TOMBS, M.P., 1991. Solution behaviour of Chromobacter viscosum and Pseudomonas sp. lipaces: no evidence of self-association Biochemical Journal. 273, 611-613
SMITH, C.J., WATSON, C.F., MORRIS, P.C., BIRD, C.R., SEYMOUR, G.B., HARDING, S.E., GRAY, J.E., ARNOLD, C., TUCKER, G.A., SCHUCH, W. and GRIERSON, D., 1990. Inheritance and effect on ripening of antisense polygalcturonase genes in transgenic tomatoes Plant Molecular Biology. 14, 369-379
MANNION, R.O., MELIA, C.D., MITCHELL, J.R., HARDING, S.E. and GREEN, A.P., 1990. An explanation of the rheological behaviour of mixed aqueous solutions of anionic and non-anionic cellulose ethers Journal of Pharmacy and Pharmacology. 42, 23P
HALL, L.R., HARDING, S.E. and WAITES, W.M., 1990. Use of bioluminescence to study heat resistance of spores of B. megaterium KM Journal of Applied Bacteriology. 69, xxiv
HARDING, S.E., MORGAN, P.J. and PETRAK, K., 1990. Low-speed flotation equilibrium of macromolecules in the analytical ultracentrifuge Journal of Physical Chemistry. 94, 978-980
MANNION, R.O, MITCHELL, J.R, HARDING, S.E and LEE, N.E. AND MELIA, C.D., 1990. A rheological investigation of some polysaccharide interactions Gums and Stabilisers for the Food Industry. 5, 451-457
BYRON, O and HARDING, S. AND RHIND, S., 1990. A comparison of the hydrodynamic properties of two novel monoclonal antibodies: B72.3 and chimeric B72.3c In: 10th International Biophysics Congress (Vancouver). P2.2.20
HALL, L.R and HARDING, S.E. AND WAITES, W.M., 1990. Use of bioluminescence to study heat resistance of spores of Bacillus megaterium KM Journal of Applied Bacteriology. 69, xxiv
MORGAN, P.J., HARDING, S.E. and PETRAK, K., 1990. Interactions of a model block copolymer drug delivery system with two serum proteins and myoglobin Biochemical Society Transactions. 18, 1021-1022
WELLS, C., MOLINA-GARCIA, A.D., HARDING, S.E. and ROWE, A.J., 1990. Self-interaction of dynein from tetrahyamena cilia Journal of Muscle Research and Cell Motility. 11, 344-350
BALL, A and HARDING, S.E. AND SIMPKIN, N.J., 1990. On the molecular weight distribution of dextran T-500 Gums and Stabilisers for the Food Industry. 5, 447-450
SIMPKIN, N.J., HARDING, S.E. and TOMBS, M.P., 1990. Hydrodynamic characterisation of Chromobacter viscosum lipase Biochemical Society Transactions. 18, 1031
DAVIS, K.G., GLENNIE, M., HARDING, S.E. and BURTON, D.R., 1990. A model for the solution conformation of rat Ige Biochemical Society Transactions. 18, 935-936
MOLINA GARCIA, A and FRASER, R.S.S. AND HARDING, S.E., 1990. A comparitive physical study of particles of tombus viruses in swollen and compact states In: 10th International Biophysics Congress (Vancouver). P2.9.1
MOLINA-GARCIA, A.D., HARDING, S.E. and FRASER, R.S., 1990. Physicochemical studies on Tomato Bushy Stunt Virus variants Biopolymers. 29, 1443-1552
BYRON, O., HARDING, S.E. and RHIND, S.K., 1990. A preliminary investigation of the hydrodynamic properties of two novel monoclonal antibodies Biochemical Society Transactions. 18, 1030-1031
MORGAN, P.J., HARDING, S.E. and PETRAK, K., 1990. Hydrodynamic properties of a polyisoprene/polyoxyethylene block polymer Macromolecules. 23, 4461-4464
HARDING, S.E, BERTH, G and BALL, A. AND MITCHELL, J.R., 1990. Hydrodynamic evidence for an extended conformation for citrus pectins in dilute solution Gums and Stabilisers for the Food Industry. 5, 257-271
DAVISON, C.J., SMITH, K.E., HUTCHINSON, L.E., O'MULLANE, J.E., HARDING, S.E., BROOKMAN, L. and PETRAK, K., 1990. Physical & biological properties of water soluble polyelectrolyte complexes Journal of Bioactive and Compatible Polymers. 5, 267-283
MANNION, R.O., MELIA, C.D., HARDING, S.E., MITCHELL, J.R. and GREEN, A.P., 1989. Predicting the viscosity of mixtures of polysaccharide thickening agents in aqueous solution Journal of Pharmacy and Pharmacology. 41, 15P
HARDING, S.E., 1989. The macrostructure of mucus glycoproteins in solution Advances in Carbohydrate Chemistry and Biochemistry. 47, 345-381
ANDERSON, M.T and HARDING, S.E. AND DAVIS, S.S., 1989. On the interaction in solution of a candidate mucoadhesive polymer, diethylaminoethyl-dextran, with pig gastric mucus glycoprotein Biochemical Society Transactions. 17, 1101-1102
HARDING, S.E. and ROWE, A.J., eds., 1989. Dynamic Properties of Biomolecular Assemblies Royal Society of Chemistry, Cambridge.
MARSH, E.N, HARDING, S.E and LEADLEY, P.F., 1989. Subunit interactions in Propionibacterium shermanii methylmalonyl-CoA mutase studied by analytical ultracentrifugation Biochemical Journal. 260, 353-358
HARDING, S.E., 1989. Modelling the gross conformation of assemblies using hydrodynamics. In: HARDING, S.E. AND ROWE, A.J., ed., Dynamic Properties of Biomolecular Assemblies Royal Society of Chemistry, Cambridge. 32-56
MOLINA-GARCIA, A.D, HARDING, S.E, DE PIERI, L and JAN, N. AND WAITES, W.M., 1989. Dynamic light scattering studies on the effect of heat and disinfectants on spores of Bacillus cereus Biochemical Journal. 263, 883-888
BRADLEY, T.D, BALL, A and HARDING, S.E. AND MITCHELL, J.R., 1989. The thermal degradation of guar gum Carbohydrate Polymers. 10, 205-214
BERTH, G and DAUTZENBERG, H. AND HARDING, S.E., 1989. The calibration of GPC for pectins In: Proc. EUROCARB 5: 5th European Symposium on Carbohydrates, Prague..
HARDING, S.E. AND TOMBS, M.P., 1989. Protein diffusion through interfaces. In: SCHWENKE, K.D. AND RAAB, B., ed., Interactions in Protein Systems Abhandlungen der Akademie der Wissenschaften der DDR, Akademie-Verlag, Berlin. 65-72
ROWE, A.J, WYNNE-JONES, S and THOMAS, D. AND HARDING, S.E., 1989. Analysis of solute concentration and concentration derivative distribution by means of frameshift Fourier and other algorithms applied to Rayleigh interferometric and Fresnel fringe patterns In: SPIE Vol 1163 Fringe Pattern Analysis. 138-148
CHIN, T.K and HARDING, S.E. AND EAGLES, P.A.M., 1989. Characterisation of two proteolytically derived soluble polypeptides from the neurofilament triplet components NFM and NFH Biochemical Journal. 264, 53-60
WAITES, W.M, HARDING, S.E, FOWLER, D.H, JONES, S.H and SHAW, D. AND MARTIN, M., 1988. Destruction of bacterial spores by hydrogen peroxide and ultraviolet light In: Proc. 10th Int. Spore Conf (Woods Hole, Mass.). 65
WAITES, W.M, HARDING, S.E, FOWLER, D.H, JONES, S.H and SHAW, D. AND MARTIN, M., 1988. The destruction of spores of Bacillus subtilis by the combined effects of hydrogen peroxide and ultraviolet light Letters in Applied Microbiology. 71, 139-140 (In Press.)
HARDING, S.E. AND WILLIAMS, K.P., 1988. Further evidence for structural homology of pyruvate ferrodoxin oxidoreductases: sedimentation velocity behaviour International Journal of Biological Macromolecules. 10, 318-319
TOMBS, M.P. AND HARDING, S.E., 1988. Protein transport processes in the water-water interface in incompatible two phase systems. In: FISHER, D. AND SUTHERLAND, I.A., ed., Advances in Separations using Aqueous Phase Systems in Cell Biology and Biotechnology Plenum Press, New York. 229-232
HARDING, S.E., 1988. Polysaccharide molecular weight determination: which technique? Gums and Stabilisers for the Food Industry. 4, 15-23
BALL, A and HARDING, S.E. AND MITCHELL, J.R., 1988. Combined low-speed sedimentation equilibrium/ gel permeation chromatography approach to molecular weight distribution analysis International Journal of Biological Macromolecules. 10, 259-264
HARDING, S.E and ROWE, A.J., 1988. Automatic data capture and analysis of Rayleigh Interference Displacements in Analytical Ultracentrifugation" Optics and Lasers in Engineering. 8, 15-23
JAN, N.A, HARDING, S.E and MOLINA-GARCIA, A. AND WAITES, W.M., 1988. A combined quasi-elastic light scattering and electron microscopic study on the stability of Bacillus cereus spores In: Proc. 10th Int. Spores Conf. (Woods Hole, Mass.). 69
SEYMOUR, G.B., HARDING, S.E., TAYLOR, A.J., HOBSON, G.E. and TUCKER, G.A., 1987. Polyuronide solubilization during ripening of normal and mutant tomato fruit Phytochemistry. 26, 1871-1875
HARDING, S.E., 1987. A general method for modelling macromolecular shape in solution. A graphical (Π - G) intersection procedure for triaxial ellipsoids Biophysical Journal. 51, 673-680
HARDING, S.E and ROWE, A.J. AND SHAW, W.V., 1987. The molecular mass and trimeric nature of chloramphenicol transacetylase Biochemical Society Transactions. 15, 513
MOLINA GARCIA, A and HARDING, S.E. AND FRASER, R.S.S., 1987. A comparative study on the physico-chemical properties of tomato bushy stunt virus In: Proc. 7th International Biophysics Conference, Jerusalem. 94
HARDING, S.E. AND ROWE, A.J., 1987. Rayleigh interferometric fringe patterns from sedimentation equilibrium experiments: use of an LKB Ultroscan for data capture and of a simple Fourier deconvolution algorithm for data analysis. In: TYRER, J., ed., Proceedings of the Automatic Fringe Analysis Symposium Open Tech. Press, Loughborough. 189-200
SEYMOUR, G.B. AND HARDING, S.E., 1987. Analysis of the molecular size of tomato (Lycopersicon esculentum Mill) fruit polyuronides by gel filtration and low-speed sedimentation equilibrium Biochemical Journal. 245, 463-466
HARDING, S.E, KYLES, P and WEST, G. AND NORTON, G., 1987. The relative molecular mass, heterogeneity and subunit composition of the 12S globulin from oil seed rape Biochemical Society Transactions. 15, 684
HARDING, S.E. AND ROWE, A.J., 1987. High precision automated off-line analysis of sedimentation equilibrium data Biochemical Society Transactions. 17, 1046-1047
HARDING, S.E., 1986. Applications of light scattering in microbiology Biotechnology & Appl. Biochem.. 8, 489-509
HARDING, S.E., 1986. A combined transient electric birefringence and excluded volume approach to macromolecular shape" Biochemical Society Transactions. 14, 857-858
GAINSFORD, S.E, HARDING, S.E and MITCHELL, J.R. AND BRADLEY, T.D., 1986. A comparison between the hot and cold water soluble fractions of two locust bean gum samples Carbohydrate Polymers. 6, 423-442
MITCHELL, J, BACK, H, GREGSON, K and HARDING, S.E. AND MATHER, S., 1986. Optimisation of products and processes Royal Society of Chemistry Special Publication. 56, 236-250
HARDING, S.E. AND BARDSLEY, R.G., 1986. The molecular weight of one of the largest known polypeptides: titin Macromolecular Preprints. 146
HARDING, S.E. AND JOHNSON, P., 1986. The Brownian diffuson of dormant and germinating spores of Bacillus megaterium J. Applied Bacteriology. 60, 227-232
HARDING, S.E., 1986. General ellipsoid modelling of macromolecular shape using light scattering and viscometry Macromolecular Preprints. 145
HARDING, S.E., 1985. The representation of equilibrium solute distributions for non-ideal polydisperse systems in the analytical ultracentrifuge Biophysical Journal. 47, 247-250
HARDING, S.E. AND JOHNSON, P., 1985. Physico-chemical studies on turnip yellow mosaic virus: homogeneity, molecular weights, hydrodynamic radii and concentration dependence of parameters Biochemical Journal. 231, 549-555
RALLISON J.M. AND HARDING, S.E., 1985. Excluded volumes for pairs of triaxial ellipsoids at dominant Brownian motion Journal of Colloid and Interface Science. 103, 284-289
HARDING, S.E. AND JOHNSON, P., 1985. The concentration dependence of macromolecular parameters Biochemical Journal. 231, 543-547
HARDING, S.E., 1984. An analysis of the heterogeneity of mucins Biochemical Journal. 219, 1061-1064
HARDING, S.E. AND JOHNSON, P., 1984. Quasi-elastic light scattering studies on dormant and germinating Bacillus subtilis spores Biochemical Journal. 220, 117-123
HALLETT, P and ROWE, A.J. AND HARDING, S.E., 1984. A highly expanded spheroidal model for a mucus glycoprotein from a cystic fibrosis patient: new evidence from electron microscopy Biochemical Society Transactions. 12, 878-879
HARDING, S.E. AND JOHNSON, P., 1984. Photon correlation spectroscopy as a probe for bacterial spore germination IRCS Med. Sci.. 12, 200-201
HARDING, S.E. AND ROWE, A.J., 1984. Erratum: Modeling biological macromolecules in solution II: the general triaxial ellipsoid Biopolymers. 23, 843
HARDING, S.E. AND ROWE, A.J., 1983. Modeling biological macromolecules in solution II:the general triaxial ellipsoid Biopolymers. 22, 1813-1829
HARDING, S.E and ROWE, A.J. AND CREETH, J.M., 1983. Further evidence for a flexible and highly expanded spheroidal model for mucus glycoproteins in solution Biochemical Journal. 209, 893-896
HARDING, S.E. AND UKABAM, S.O., 1983. Mathematical models for determining the intestinal permeability using polyethylene glycol Gut. 24, 456
HARDING, S.E and CREETH, J.M. AND ROWE, A.J., 1983. Modelling the conformation of mucus glycoproteins in solution In: Proceedings 7th International Glycoconjugates Conference (editors A. Chester, D. Heinegard, A. Lundblad and S. Svensson), Olsson Reklambyra, Sweden. 558-559
HARDING, S.E., 1983. Triaxial ellipsoids as models for macromolecules in solution: procedures for numerical inversion of the shape functions leading to a stable, unique solution Comput. Biol. Med. 13, 89-97
HARDING, S.E. AND CREETH, J.M., 1983. Polyelectrolyte behaviour in mucus glycoproteins Biochim. et Biophys. Acta. 746, 114-119
HARDING, S.E and DAMPIER, M.J. AND ROWE, A.J., 1982. The viscosity increment for ellipsoids of revolution: Some observations on the Simha formula Biophysical Chemistry. 15, 205-208
CREETH, J.M. AND HARDING, S.E., 1982. A simple test for macromolecular heterogeneity in the analytical ultracentrifuge Biochemical Journal. 205, 639-641
ROWE, A.J. AND HARDING, S.E., 1982. The conformation of C-protein in solution: use of new hydrodynamic shape functions J. Muscle Res. and Cell Motility. 3, 488
CREETH, J.M. AND HARDING, S.E., 1982. Some observations on a new type of point average molecular weight J. Biochem. Biophys. Methods. 7, 25-34
HARDING, S.E. AND CREETH, J.M., 1982. Self association, polydispersity and non-ideality in a cystic fibrotic glycoprotein IRCS Med. Sci.. 10, 474-475
HARDING, S.E. AND ROWE, A.J., 1982. Modelling biological macromolecules in solution III: the Λ - R intersection method for triaxial ellipsoids International Journal of Biological Macromolecules. 4, 357-361
HARDING, S.E., 1982. A computer program for evaluating the hydrodynamic parameters for a macromolecule for any given value of its axial dimensions Comput. Biol. Med.. 12, 75-80
HARDING, S.E. AND ROWE, A.J., 1982. Modelling biological macromolecules in solution I: the ellipsoid of revolution model International Journal of Biological Macromolecules. 4, 160-164
HARDING, S.E and DAMPIER, M.J. AND ROWE, A.J., 1981. The viscosity increment of a dispersion of triaxial ellipsoids under dominant Brownian motion Journal of Colloid and Interface Science. 79, 7-13
HARDING, S.E., 1981. A compound hydrodynamic shape function derived from viscosity and molecular covolume measurements International Journal of Biological Macromolecules. 3, 340-341
HARDING, S.E., 1981. Could egg albumin be egg shaped? International Journal of Biological Macromolecules. 3, 398-399
HARDING, S.E., 1980. Modelling Biological Macromolecules in Solution. The General Triaxial Ellipsoid: PhD Dissertation University of Leicester.
HARDING, S.E., 1980. Viscosity parameters for myoglobin IRCS Medical Science. 8, 610
HARDING, S.E., 1980. The combination of the viscosity increment with the harmonic mean relaxation time for determining the conformation of biological macromolecules in solution Biochemical Journal. 189, 359-361 (& corrigenda page to vol 189)
HARDING, S.E, DAMPIER, M.J and ROWE, A.J., 1979. Modelling biological macromolecules in solution: the general triaxial ellipsoid can now be employed IRCS Medical Science. 7, 33
HARDING, S.E., 1977. The improvement in Wear Resistance of Ion Implanted materials: MSc Dissertation AERE Harwell/ Brighton Polytechnic.

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