Within the group we are interested in different aspects of cellular immunology, immune modulation through surface engineering, Dendritic cell and macrophage biology and immune competent tissue models. We have a particular interest in monocytes and their dendritic cells and macrophage progeny and their cross-talk with environmental cues.
A wide range of techniques are used including primary cell cultures (including dendritic cells, macrophages, bronchial epithelial cells, keratinocytes and fibroblasts), multicolor flowcytometry, live cell sorting (magnetic and Fluorescence-activated), gene silencing (siRNA), primary cell transfection, PCR and Western Blotting. We also routinely use 3D synthetic scaffolds and hydrogels for creating biomimetic co-cultures of different cell types.
Ghaemmagahmi's group research spans three interdisciplinary themes:
1. Immune modulation and promotion of healing through surface engineering: Using 'Bio-Instructive' materials to modulate the functional properties and differentiation of immune cells (e.g. macrophages and dendritic cells) and other cell types (e.g. epithelial cells and fibroblasts) involved in inflammation, wound healing and fibrosis. Also see
2. Novel immune-competent biomimetic tissue models: Engineering of physiologically relevant tissues for disease modelling, testing drug leads and toxicity experiments.
3. Host - Environment interaction: Elucidating early events at the interface of antigen presenting cells and epithelial cells with airborne allergens/pathogens
Our work has received support from EPSRC, Asthma UK, BBSRC, MRC, Unilever, NC3Rs and European Commission with excess of £13M funding secured since 2006 as either PI or Co-I.
Main ongoing projects include:
- Local immune-modulation around implants by innovative auxiliary hydrogel-based systems
- Bio-instructive Materials for Immune Modulation
- Modulating macrophage function through surface modification
- Immune modulatory properties of nano-particles
- Construction of 3D immune-competent barrier tissue models (skin and lung)
- Novel 3D culture systems for studying innate-adaptive immune cells interaction
- The role of c-type lectin receptors in allergic sensitisation
- IDO activity in human Dendritic cells and epithelial cells
- In vitro models of human lung fibrosis
- Role of mechanosensors in lung fibrosis
- Perfusion bioreactors for epithelial cell culture
HTWE SS, CHA BH, YUE K, KHADEMHOSSEINI A, KNOX AJ and GHAEMMAGHAMI AM, 2017. Role of Rho-Associated Coiled-Coil Forming Kinase Isoforms in Regulation of Stiffness-Induced Myofibroblast Differentiation in Lung Fibrosis. American journal of respiratory cell and molecular biology. 56(6), 772-783
SALAZAR, FABIAN, SEWELL, HERB F, SHAKIB, FAROUK and GHAEMMAGHAMI, AMIR M, The role of lectins in allergic sensitization and allergic disease. The Journal of allergy and clinical immunology. 132(1), 27-36
CHA BH, SHIN SR, LEIJTEN J, LI YC, SINGH S, LIU JC, ANNABI N, ABDI R, DOKMECI MR, VRANA NE, GHAEMMAGHAMI AM and KHADEMHOSSEINI A, 2017. Integrin-Mediated Interactions Control Macrophage Polarization in 3D Hydrogels. Advanced healthcare materials.