Assistant Professor, Faculty of Medicine & Health Sciences
B.Sc. University of Wales (1990); Ph.D. University of Edinburgh (1996); Postdoctoral Research Fellow, University of Nottingham (1996-1999); Research Fellow, The Natural History Museum, London (1999-2001); Lecturer, University of Nottingham (2001-present). Visiting Professor, Chulalongkorn University, Bangkok (2010-present)
The research in my lab focuses on using DNA and protein sequences to build molecular phylogenies in order to investigate evolutionary relationships among organisms and to answer questions in… read more
EVANS T, WADE CM, CHAPMAN FA, JOHNSON AD and LOOSE M, 2014. Acquisition of germ plasm accelerates vertebrate evolution. Science (New York, N.Y.). 344(6180), 200-3 NANTARAT, N., TONGKERD, P., SUTCHARIT, C., WADE, C. M., NAGGS, F. and PANHA, S., 2013. Phylogenetic relationships of the operculate land snail genus Cyclophorus Montfort, 1810 in Thailand Molecular Phylogenetics and Evolution. 70, 99-111 DARLING, K.F., THOMAS, E., KASEMANN, S.A., SEEARS, H.A., SMART, C.W. and WADE, C.M., 2009. Surviving mass extinction by bridging the benthic/planktic divide Proceedings of the National Academy of Sciences of the United States of America. 106(31), 12629-12633
DARLING,K.F., WADE,C.M., STEWART,I.A., KROON,D., DINGLE,R. and LEIGHBROWN,A.J., 2000. Molecular evidence for genetic mixing of Arctic and Antarctic subpolar populations of planktonic foraminifers Nature. 405(6782), 43-47
The research in my lab focuses on using DNA and protein sequences to build molecular phylogenies in order to investigate evolutionary relationships among organisms and to answer questions in evolutionary biology, taxonomy and biogeography.
Our research falls in 4 main areas:
1) Evolutionary relationships of the pulmonate land snails and slugs
2) Evolutionary relationships in the planktonic foraminifera
3) Snails as intermediate hosts for medically important parasites
1) Evolutionary Relationships in the Pulmonate Land Snails and Slugs (Pulmonata: Stylommatophora)
Land snails and slugs represent one of the largest invasions of the land, comprising some 30,000-35,000 species. They are major components of many ecosystems, and they include the vectors of many serious diseases. They have also become important models for studies on the mechanisms of evolution and are eminently suitable as subjects for biogeographic studies of early tectonic events.
Evolutionary Relationships and Taxonomy
Until recently, the origins and the deep-level evolutionary relationships of the major groupings of the land snails and slugs remained virtually unknown. Anatomical studies gave confusing and conflicting results, such that there appeared to be almost as many classifications as there were classifiers.
Working in the lab
Our recent molecular work has begun to unravel the evolutionary relationships within the land snails and slugs. We have sequenced part of the ribosomal RNA gene cluster for some 260 species of land snails and slugs in the Pulmonate suborder Stylommatophora. Although our molecular phylogeny largely agrees with the traditional taxonomy at the level of families, at deeper levels the molecular tree markedly disagrees with the traditional taxonomy. Remarkably, the Orthurethra (previously considered to be ancestral) appear to be relatively advanced, indicating that supposedly primitive features such as the orthurethran kidney are in fact derived. This finding is leading to a radical reinterpretation of early land snail evolution.
Adult Achatina achatina and baby
We are currently extending our molecular work on the Stylommatophora to include more taxa and to obtain new genes in order to provide independent confirmation of the relationships in the rDNA tree. In addition, we are expanding our phylogeny to include other non-stylommatophoran pulmonate taxa. We ar
Recent genetic studies of the foraminifera have provided new insights into the evolution of the group. Our comparisons of small subunit (SSU) ribosomal (r) RNA sequences of the foraminifera with those of other eukaryotes have shown that the foraminifera apparently form one of the earliest diverging eukaryote lineages in the 'tree of life'. More work is needed to confirm this placement as the fast rate of evolution observed in foraminiferal SSU rRNA genes renders the group difficult to place in evolutionary trees. Our genetic studies of the foraminifera have also provided a great deal of information about evolutionary relationships within the group. Interestingly, the evolutionary transition between a benthic and a planktonic way of life appears to have occurred several times. Furthermore, planktonic spinose species (foraminiferans with spines) cluster separately from the planktonic non-spinose species. We are currently undertaking new molecular studies of the foraminifera utilising both the SSU rRNA gene and other nuclear genes in order to further investigate their placement in the 'tree of life' and the evolutionary relationships within the group. One of the most interesting outcomes of genetic studies of the planktonic foraminifera concerns the extent of differentiation within individual morphospecies. We have shown that most mophospecies of planktonic foraminifera show an exceptionally high level of genetic diversity in their SSU rRNA genes, and that many include more than one genetically distinct entity. Indeed, some of these genetic types may warrant classification as separate 'cryptic' species. This finding is of interest because of the role of foraminiferal microfossils in reconstructing past climates. For climate reconstruction it has been assumed that each 'morphospecies' is a single entity with a specific ecological (and thus climatic) preference. If the distinct genetic types within morphospecies are in fact adapted to different habitats, and exhibit different ecological and climate preferences, then the assumption that each morphospecies is characteristic of a particular climate would be wrong. If this is so, there may be significant errors in current models of climate reconstruction. Recent work suggests that different genetic types are indeed associated with different environments. If it does become possible to distinguish these newly recognised genetic types in the fossil record, the role of the foraminifera as indicators of past climate could be greatly enhanced. Land snails and slugs represent one of the largest invasions of the land, comprising some 30,000-35,000 species. They are major components of many ecosystems, and they include the vectors of many serious diseases. They have also become important models for studies on the mechanisms of evolution and are eminently suitable as subjects for biogeographic studies of early tectonic events.