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Paul Dyer

Professor of Fungal Biology, Faculty of Medicine & Health Sciences

Contact

  • workRoom B73 Office or B85 Laboratory Life Science Building
    University Park
    Nottingham
    NG7 2RD
    UK
  • work0115 951 3203
  • fax0115 951 3251

Biography

BA University of Cambridge 1987; PhD University of Cambridge 1991; Research Associate, University of Nottingham 1991-1996; BBSRC David Phillips Research Fellow 1996-2001; Australian Antarctic Division Expeditioner 1999-2000; Lecturer, University of Nottingham 2002-present. ŒBerkeley Award¹ (1999) from British Mycological Society for outstanding contribution to mycology/pathology research.

Research Summary

Sexual Reproduction in Fungi

Investigation of molecular and physiological processes controlling sexual reproduction in ascomycete fungi. The overall aim is to understand the evolution of sexuality and asexuality in fungi, both from a fundamental and a practical perspective such that the sexual cycle can be manipulated as a valuble tool for species of biotechnological and medical significance. A variety of experimental techniques are being used, including those of classical genetics, molecular biology and genomics. Particular aims are (a) to identify mating-type (MAT) genes determining sexual compatibility from plant and human pathogenic fungi and species used in biotechnology, results will be of use in studies of mating-type distribution, design of diagnostic kits, and understanding of the role of MAT genes in sexuality ; (b) to identify downstream genes involved with sexual reproduction such as pheromone and signaling pathway genes, and assess their role, together with MAT genes, in the evolution of asexuality in fungi; (c) to investigate hormonal factors triggering and co-ordinating the sexual cycle and determine whether they may be useful as fungal growth regulators. Current studies are focused on species from the genus Aspergillus and Penicillium, with an especial interest in the role of the sexual cycle in the evolution of the opportunistic pathogen Aspergillus fumigatus.

Sexual reproductive apothecia of the cereal pathogen Tapesia

Sexual ascospores of Aspergillus nidulans (S. Champe)

Population Biology of Fungi including Lichens

Molecular markers such as randomly amplified polymorphic DNA (RAPDs), specific gene sequences, and ribosomal DNA sequences are being used to examine breeding systems and the extent of population variation within species of pathogenic fungi and lichens. DNA fingerprinting studies are in progress with temperate plant pathogens, medical pathogens, species used in the biotechnology, and lichens from both temperate regions and the Antarctic (in collaboration with Drs. P. Crittenden and M. Dickinson). Results are of signifcance in understanding biodiversity within fungal populations, with possible implications for the ability of species to withstand climate change.

The lichens Buellia frigida and Caloplaca saxicola from the Antarctic

Example of DNA fingerprinting of lichens using RAPD PCR

The importance of non-genotypic variation is being assessed for the survival of yeast populations subject to environmental stressors (in collaboration with Dr Simon Avery)

Fungicide Resistance and Molecular Diagnostics in the Cereal Eyespot Fungus Tapesia

Tapesia species are responsible for causing eyespot disease of cereals which can lead to a significant loss in yield. Studies are in progress to determine the molecular genetic basis of fungicide resistance in these fungi, to develop species-specific molecular diagnostic markers, and to study the molecular basis of evolution of Tapesia species.

Classic symptoms of eyespot disease on stem base of wheat plant

Phenotypic variation in sexual progeny of Tapesia species

Fungal Genomics

Newly available fungal genomic resources are being used for bioinformatic and post-genomic experimental work, in order to investigate the genetic basis of traits of interest such as genes controlling sexual developmental processes. In addition the USA Joint Genomes Institute has provide funding to allow the genome sequencing, for the first time, of a lichen forming fungus. The genome programme for Xanthoria parietina is being co-ordinated from Nottingham (in collaboration with Prof David Archer and Dr Peter Crittenden).

lichen

Thallus of the lichen Xanthoria parietina

Acknowledgements Work is supported by project grants from the Biotechnology and Biological Sciences Research Council (UK), the Natural Environment Research Council (UK), the Wellcome Trust, and the European Union.

Research Collaborators Dr Peter Crittenden, Dr Matt Dickinson, Prof. David Archer, Dr Simon Avery.

Selected Publications

Future Research

(1) Genomics of the lichen-forming fungus Xanthoria parietina. Assistance is welcomed in the annotation and exploitation of the genome sequence.

(2) Adaptation of Antarctic lichens. Collaboration over future work to understand genetic adaptions in polar lichens is welcomed.

(3) Genetic traits of medical importance in the opportunistic pathogen Aspergillus fumigatus. Collaborations involving the use of the sexual cycle to assess the nature of traits such as virulence and fungicide resistance are welcomed.

School of Life Sciences

University of Nottingham
Medical School
Queen's Medical Centre
Nottingham NG7 2UH

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