Robert Markus

Contact

• workRoom C55, C72 CBS
  University Park
  Nottingham
  NG7 2RD
  UK
• work1158232021
• Robert.Markus@nottingham.ac.uk

Biography

Work experience

• 2014 - present , Senior Imaging Technician, Super Resolution Microscopy, School of Life Sciences, Univ. of Nottingham
• 2011 - 2014 Postdoctoral researcher, Stockholm University, Sweden
• 2006 - 2010 Young Investigator Fellow, BRC-Szeged, Hungarian Academy of Science

Education

• 2006 PhD, University of Szeged, Hungary
• 2001 MSc Biology, Babes-Bolyai University, Cluj, Romania

Additional training & Workshops

• ATP - associate teachers program - completed, UoN

Microscopy related meetings workshops

• Frontiers of BioImaging Workshops, UK
• MMC of Royal Microscopical Society, UK
• Super Resolution Imaging Workshops, Leeds, UK
• Elyra Super Resolution Meetings, UK
• Advanced Super resolution Course, Jena Germany (2016)
• Microscopy Workshops & Demos
• Super-resolution workshop, Edinburgh, UK (2014)
• Super-resolution workshop, SciLifeLab, Sweden (2014)

Other

• awarded images at Nikon Small World, Royal Microscopical society, EMBO coverpage

Expertise Summary

Microscopy techniques: Super resolution SIM, STORM, PALM, Advanced fluorescence: FRAP, FRET, TIRF, Confocal microscopy, two photon microscopy, wide field fluorescence, live cell imaging, bright field: Polarisation, DIC, Phase Contrast, histology sections, macroscopy, low magnification reproduction techniques, micro studio lightning techniques, image processing and analysis

Other technical skills and experience: FACS (cell sorter), immunohistochemistry, immunofluorescence, Western Blot, Immune precipitation, Infection assays (bacterial, nematodes, parasitoid wasps), cell lab techniques, drosophila genetics, nematode maintenance, stem cells, plant histology.

Other: photography, videography, image and movie editing

Teaching Summary

One to one teaching : training users to use confocal, super resolution microscopes, image processing and analysis, figure preparation Courses& Seminars on microscopy for Undergraduate and… read more

Research Summary

2014-present

• Optimization of super resolution microscopy in a multi user environment
• Development and design of test samples for super resolution microscopy
• Development of high resolution mounting media in collaboration with Citifluor, in order to improve SIM and STORM microscopy

Recent Publications

• SHERMAN HG, JOVANOVIC C, ABUAWAD A, KIM DH, COLLINS H, DIXON JE, CAVANAGH R, MARKUS R, STOLNIK S and RAWSON FJ, 2019. Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types. Biochimica et biophysica acta. Bioenergetics. (In Press.)
• WOODHAMS, STEPHEN G., MARKUS, ROBERT, GOWLER, PETER R.W., SELF, TIMOTHY J. and CHAPMAN, VICTORIA, 2019. Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain: PAIN Pain. Articles in Press,
• DE MELO N, MCGINLAY S, MARKUS R, MACRI-PELLIZZERI L, SYMONDS ME, AHMED I and SOTTILE V, 2019. Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells. Biomimetics (Basel, Switzerland). 4(3),
• WILLSON BJ, KOVÁCS K, WILDING-STEELE T, MARKUS R, WINZER K and MINTON NP, 2016. Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels. 9, 109

• View all publications

• One to one teaching : training users to use confocal, super resolution microscopes, image processing and analysis, figure preparation
• Courses& Seminars on microscopy for Undergraduate and Doctoral Training Program students

Past Research

2011-2014

• Insect pathogens, naturally occurring infection systems (Entomopathogenic nematode infections of Drosophila)
• Analysis of RNA sequencing data of RAS induced salivary gland tumours in Drosophila and investigation of cellular immune response towards tumours (microscopy approach)
• Ecological immunology - design, develop methods to investigate cellular immune response of leaf beetles, across Sweden, towards parasitoid wasps.

2001-2011

• Immunology, Innate immunity, and blood cell development in Drosophila melanogaster and Drosophila ananassae (PhD years and Young investigator fellowship), Szeged, Hungary
• Discovery of new hematopoietic tissue and discovery of multinucleated giant blood cells in D ananassae.
• Discovery of new phagocytosis receptor family (Nimrod genes)

• SHERMAN HG, JOVANOVIC C, ABUAWAD A, KIM DH, COLLINS H, DIXON JE, CAVANAGH R, MARKUS R, STOLNIK S and RAWSON FJ, 2019. Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types. Biochimica et biophysica acta. Bioenergetics. (In Press.)
• WOODHAMS, STEPHEN G., MARKUS, ROBERT, GOWLER, PETER R.W., SELF, TIMOTHY J. and CHAPMAN, VICTORIA, 2019. Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain: PAIN Pain. Articles in Press,
• DE MELO N, MCGINLAY S, MARKUS R, MACRI-PELLIZZERI L, SYMONDS ME, AHMED I and SOTTILE V, 2019. Live Simultaneous Monitoring of Mineral Deposition and Lipid Accumulation in Differentiating Stem Cells. Biomimetics (Basel, Switzerland). 4(3),
• WILLSON BJ, KOVÁCS K, WILDING-STEELE T, MARKUS R, WINZER K and MINTON NP, 2016. Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. Biotechnology for Biofuels. 9, 109
• 2016. Geographic variation and trade-offs in parasitoid virulence. The Journal of animal ecology. (In Press.)
• DUNAJOVÁ L, CASH E, MARKUS R, ROCHETTE S, TOWNLEY AR and WHEATLEY SP, 2016. The N-terminus of survivin is a mitochondrial-targeting sequence and Src regulator. Journal of cell science. 129(14), 2707-12
• MÁRKUS R, LERNER Z, HONTI V, CSORDÁS G, ZSÁMBOKI J, CINEGE G, PÁRDUCZ Á, LUKACSOVICH T, KURUCZ É and ANDÓ I, 2015. Multinucleated Giant Hemocytes Are Effector Cells in Cell-Mediated Immune Responses of Drosophila. Journal of innate immunity. 7(4), 340-53
• FORS L, MARKUS R, THEOPOLD U and HAMBÄCK PA, 2014. Differences in cellular immune competence explain parasitoid resistance for two coleopteran species. PloS one. 9(9), e108795
• HAULING T, KRAUTZ R, MARKUS R, VOLKENHOFF A, KUCEROVA L and THEOPOLD U, 2014. A Drosophila immune response against Ras-induced overgrowth. Biology open. 3(4), 250-60
• KARI B, ZSÁMBOKI J, HONTI V, CSORDÁS G, MÁRKUS R, ANDÓ I and KURUCZ É, 2013. A novel method for the identification of factors involved in host-pathogen interactions in Drosophila melanogaster. Journal of immunological methods. 398-399, 76-82
• AREFIN B, KUCEROVA L, DOBES P, MARKUS R, STRNAD H, WANG Z, HYRSL P, ZUROVEC M and THEOPOLD U, 2013. Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins. Journal of innate immunity. 6(2), 192-204
• LIPINSZKI Z, KLEMENT E, HUNYADI-GULYAS E, MEDZIHRADSZKY KF, MÁRKUS R, PÁL M, DEÁK P and UDVARDY A, 2013. A novel interplay between the ubiquitin–proteasome system and serine proteases during Drosophila development. The Biochemical journal. 454(3), 571-83
• HONTI V, CSORDÁS G, KURUCZ É, MÁRKUS R and ANDÓ I, 2013. The cell-mediated immunity of Drosophila melanogaster: hemocyte lineages, immune compartments, microanatomy and regulation. Developmental and comparative immunology. 42(1), 47-56
• DOBES P, WANG Z, MARKUS R, THEOPOLD U and HYRSL P, 2012. An improved method for nematode infection assays in Drosophila larvae. Fly. 6(2), 75-9
• HONTI V, CSORDÁS G, MÁRKUS R, KURUCZ E, JANKOVICS F and ANDÓ I, 2010. Cell lineage tracing reveals the plasticity of the hemocyte lineages and of the hematopoietic compartments in Drosophila melanogaster. Molecular immunology. 47(11-12), 1997-2004
• HONTI V, KURUCZ E, CSORDÁS G, LAURINYECZ B, MÁRKUS R and ANDÓ I, 2009. In vivo detection of lamellocytes in Drosophila melanogaster. Immunology letters. 126(1-2), 83-4
• MÁRKUS R, LAURINYECZ B, KURUCZ E, HONTI V, BAJUSZ I, SIPOS B, SOMOGYI K, KRONHAMN J, HULTMARK D and ANDÓ I, 2009. Sessile hemocytes as a hematopoietic compartment in Drosophila melanogaster. Proceedings of the National Academy of Sciences of the United States of America. 106(12), 4805-9
• KURUCZ E, MÁRKUS R, ZSÁMBOKI J, FOLKL-MEDZIHRADSZKY K, DARULA Z, VILMOS P, UDVARDY A, KRAUSZ I, LUKACSOVICH T, GATEFF E, ZETTERVALL CJ, HULTMARK D and ANDÓ I, 2007. Nimrod, a putative phagocytosis receptor with EGF repeats in Drosophila plasmatocytes. Current biology : CB. 17(7), 649-54
• RUS F, KURUCZ E, MÁRKUS R, SINENKO SA, LAURINYECZ B, PATAKI C, GAUSZ J, HEGEDUS Z, UDVARDY A, HULTMARK D and ANDÓ I, 2006. Expression pattern of Filamin-240 in Drosophila blood cells. Gene expression patterns : GEP. 6(8), 928-34
• MÁRKUS R, KURUCZ E, RUS F and ANDÓ I, 2005. Sterile wounding is a minimal and sufficient trigger for a cellular immune response in Drosophila melanogaster. Immunology letters. 101(1), 108-11
• SOAVE M, GOULDING J, MARKUS R, HILL SJ and STODDART LA, Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy. Methods in molecular biology (Clifton, N.J.). 2041, 163-181