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Robert Markus

Senior Imaging Technician, Faculty of Medicine & Health Sciences

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Biography

Work experience

  • 2014 - present , Senior Imaging Technician, Super Resolution Microscopy, School of Life Sciences, Univ. of Nottingham
  • Oct 2012 - Sept 2014 Postdoctoral researcher, Stockholm University, Neurochemistry, Sweden
  • Jan 2011 - Sept 2012 Postdoctoral researcher, Stockholm University, Molecular Biosciences WGC, Sweden
  • July 2006 - Dec 2010 Young Investigator Fellow, BRC-Szeged, Hungarian Academy of Sciences
  • Sept 2002 - Jun 2006 PhD studies at University of Szeged, Hungary
  • Oct 1997 - Jul 2001 MSc Biology, Babes-Bolyai University, Cluj, Romania
  • Sept 1993 - May 1997 Informatics - Báthory school, Cluj, Romania

Additional training & Workshops

ATP - associate teachers program - ongoing, UoN

Microscopy

  • 2016 Frontiers of BioImaging Workshop, London, UK
  • 2016 Advanced Super resolution Course, Jena Germany
  • 2015 Super Resolution Imaging Workshop, Leeds, UK
  • 2015 Microscopy Workshop and Demo, at Zeiss, Cambridge, UK
  • 2015 RMS Workshops and meeting, Manchester, UK
  • 2015 SIM imaging, OMX, Birmingham, UK
  • 2015 Interdisciplinary Microscopy Workshop, Nottingham, UK
  • 2015 Facility managers meeting, Cambridge, UK
  • 2014 Image Processing and Analysis, Leicester, UK
  • 2014 Super-resolution workshop, Edinburgh, UK
  • 2014 Super-resolution workshop, SciLifeLab, Sweden
  • 2013 Super resolution microscopy symposium, SciLifeLab, Sweden

Other

  • 2012 High throughput sequencing methods SciLifeLab, Sweden
  • 2011 Imaging and image analysis, Stockholm Univ., Sweden
  • 2010 Innovation management, Szeged University, Hungary
  • 2003 International Training Course in Bioinformatics, IBB, Poland
  • 2001 International Training Course in Modern Biology, BRC, Hungary

Expertise Summary

Microscopy techniques: Super resolution SIM, STORM, PALM, Advanced fluorescence: FRAP, FRET, TIRF, Confocal microscopy, two photon microscopy, wide field fluorescence, live cell imaging, bright field: Polarisation, DIC, Phase Contrast, histology sections, macroscopy, low magnification reproduction techniques, micro studio lightning techniques, image processing and analysis

Other technical skills and experience: FACS (cell sorter), immunohistochemistry, immunofluorescence, Western Blot, Immune precipitation, Infection assays (bacterial, nematodes, parasitoid wasps), cell lab techniques, drosophila genetics, nematode maintenance, stem cells, plant histology.

Other: photography, videography, image and movie editing

Teaching Summary

One to one teaching : training users to use confocal, super resolution microscopes, image processing and analysis, figure preparation Courses& Seminars on microscopy for Undergraduate and… read more

Research Summary

2014-present

  • Optimization of super resolution microscopy in a multi user environment
  • Development and design of test samples for super resolution microscopy
  • Development of high resolution mounting media in collaboration with Citifluor, in order to improve SIM and STORM microscopy

Recent Publications

  • One to one teaching : training users to use confocal, super resolution microscopes, image processing and analysis, figure preparation
  • Courses& Seminars on microscopy for Undergraduate and Doctoral Training Program students

Past Research

2011-2014

  • Insect pathogens, naturally occurring infection systems (Entomopathogenic nematode infections of Drosophila)
  • Analysis of RNA sequencing data of RAS induced salivary gland tumours in Drosophila and investigation of cellular immune response towards tumours (microscopy approach)
  • Ecological immunology - design, develop methods to investigate cellular immune response of leaf beetles, across Sweden, towards parasitoid wasps.

2001-2011

  • Immunology, Innate immunity, and blood cell development in Drosophila melanogaster and Drosophila ananassae (PhD years and Young investigator fellowship), Szeged, Hungary
  • Discovery of new hematopoietic tissue and discovery of multinucleated giant blood cells in D ananassae.
  • Discovery of new phagocytosis receptor family (Nimrod genes)

School of Life Sciences

University of Nottingham
Medical School
Queen's Medical Centre
Nottingham NG7 2UH

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